Click here to switch to the map view.

The map label for this gene is rfbB [C]

Identifier: 78043771

GI number: 78043771

Start: 866015

End: 866944

Strand: Direct

Name: rfbB [C]

Synonym: CHY_0979

Alternate gene names: 78043771

Gene position: 866015-866944 (Clockwise)

Preceding gene: 78044837

Following gene: 78043710

Centisome position: 36.06

GC content: 40.11

Gene sequence:

>930_bases
ATGAAGATCCTAGTAACCGGAGGAGCAGGCTTTATTGGCAGTGCCTTTGTAAGAAAGTATGCTTATGACCACGAGTTAAT
AATAGTGGACAAGCTTACCTATGCCGGAGATTTGAGAAGGATAGAAGAAGTAAGAGACCGGATAAAGTTCTATAAGGCAG
ATGTAGCAGATAAAACTGCCATAGAAGAAATCTTTGACAAAGAGAAACCAGAAGCGGTAGTCCACTTTGCAGCTGAATCC
CATGTAGACCGCAGCATCCAGGACCCAACACCTTTTATAGAAACCAACGTTAAAGGCACCCAGGTAATGCTTGATGCCAG
TAGAAAATACGGCATTGAAAAATTTGTCCATATCTCTACCGATGAAGTATACGGGGAATTAGGAAAAGAAGGACAATTTA
CCGAAGAATCACCCTTACGACCTAATTCTCCTTACTCCGTATCGAAGGCAGCAGCCGACATGTTAGCTCGGGCCTATCAC
CGGACTTATGGACTTCCGGTAATCGTAGCAAGACCGTGCAACAATTATGGGCCATGGCAGTATCCAGAAAAGCTAATCCC
TGTAGTAATCAAAAAAGCCTTAAACAATGAACCAATTCCCGTATACGGGCAAGGATTAAACGTCCGAGAATGGCTTTATG
TAGATGACTGTATAGAAGCGGTTTATCTCTTACTTCAGAAAGGCAAACCTGGAGAAGCCTACAACATCGGAAGTGGAGAA
GAAAAAGGAAATATTGAGGTGGTAAAAGAAATACTGAGAATTCTTGGAAAACCCGAGTCCTTAATTACTTTTGTTGAAGA
TAGGCCAGGACATGATTTTAGGTATTCTCTAAATTCAAAAAAGATAAAAATGAATTATGCTTGGAAACATAAAGTTAATT
TTAATGAGGGAATAAGATTTGTAATAGACTGGTATAAGAAGCATTTTTAG

Upstream 100 bases:

>100_bases
TCTTCCCGAAACCTGGCAGGAATCTACGGAAAAATATCTAAAAGAACTAAAAAAGATATAAAAAAAATAAAAAACTAAAT
AAACTTGAAAGGAGTCAAAA

Downstream 100 bases:

>100_bases
CTGTAAATTATGAATATAGAGCTATAGTTTCATGTAATAGAGGCTTTGTTAAATAAGGAGTGTAGTTAAGTTGAATAAAA
AAATATGTTTTATAACATGT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKILVTGGAGFIGSAFVRKYAYDHELIIVDKLTYAGDLRRIEEVRDRIKFYKADVADKTAIEEIFDKEKPEAVVHFAAES
HVDRSIQDPTPFIETNVKGTQVMLDASRKYGIEKFVHISTDEVYGELGKEGQFTEESPLRPNSPYSVSKAAADMLARAYH
RTYGLPVIVARPCNNYGPWQYPEKLIPVVIKKALNNEPIPVYGQGLNVREWLYVDDCIEAVYLLLQKGKPGEAYNIGSGE
EKGNIEVVKEILRILGKPESLITFVEDRPGHDFRYSLNSKKIKMNYAWKHKVNFNEGIRFVIDWYKKHF

Sequences:

>Translated_309_residues
MKILVTGGAGFIGSAFVRKYAYDHELIIVDKLTYAGDLRRIEEVRDRIKFYKADVADKTAIEEIFDKEKPEAVVHFAAES
HVDRSIQDPTPFIETNVKGTQVMLDASRKYGIEKFVHISTDEVYGELGKEGQFTEESPLRPNSPYSVSKAAADMLARAYH
RTYGLPVIVARPCNNYGPWQYPEKLIPVVIKKALNNEPIPVYGQGLNVREWLYVDDCIEAVYLLLQKGKPGEAYNIGSGE
EKGNIEVVKEILRILGKPESLITFVEDRPGHDFRYSLNSKKIKMNYAWKHKVNFNEGIRFVIDWYKKHF
>Mature_309_residues
MKILVTGGAGFIGSAFVRKYAYDHELIIVDKLTYAGDLRRIEEVRDRIKFYKADVADKTAIEEIFDKEKPEAVVHFAAES
HVDRSIQDPTPFIETNVKGTQVMLDASRKYGIEKFVHISTDEVYGELGKEGQFTEESPLRPNSPYSVSKAAADMLARAYH
RTYGLPVIVARPCNNYGPWQYPEKLIPVVIKKALNNEPIPVYGQGLNVREWLYVDDCIEAVYLLLQKGKPGEAYNIGSGE
EKGNIEVVKEILRILGKPESLITFVEDRPGHDFRYSLNSKKIKMNYAWKHKVNFNEGIRFVIDWYKKHF

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=320, Percent_Identity=40, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI42516563, Length=317, Percent_Identity=27.7602523659306, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI56237023, Length=291, Percent_Identity=28.1786941580756, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI56118217, Length=291, Percent_Identity=28.1786941580756, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI189083684, Length=291, Percent_Identity=28.1786941580756, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4504031, Length=285, Percent_Identity=24.5614035087719, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4507709, Length=327, Percent_Identity=22.3241590214067, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI48994969, Length=329, Percent_Identity=46.5045592705167, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1788353, Length=334, Percent_Identity=48.502994011976, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI1786974, Length=337, Percent_Identity=26.7062314540059, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788365, Length=329, Percent_Identity=23.1003039513678, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788366, Length=201, Percent_Identity=27.8606965174129, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=323, Percent_Identity=38.0804953560372, Blast_Score=206, Evalue=9e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=317, Percent_Identity=33.7539432176656, Blast_Score=169, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17539532, Length=314, Percent_Identity=24.5222929936306, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=345, Percent_Identity=24.3478260869565, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71982035, Length=343, Percent_Identity=24.7813411078717, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=224, Percent_Identity=24.5535714285714, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=224, Percent_Identity=24.5535714285714, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=199, Percent_Identity=25.6281407035176, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=200, Percent_Identity=25.5, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=200, Percent_Identity=25.5, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=199, Percent_Identity=25.6281407035176, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=288, Percent_Identity=28.4722222222222, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321437, Length=236, Percent_Identity=26.6949152542373, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356223, Length=321, Percent_Identity=27.1028037383178, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19923002, Length=302, Percent_Identity=29.4701986754967, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24158427, Length=268, Percent_Identity=27.6119402985075, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 35339; Mature: 35339

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAFVRKYAYDHELIIVDKLTYAGDLRRIEEVRDRIKFYKADVADKTA
CEEEEECCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IEEIFDKEKPEAVVHFAAESHVDRSIQDPTPFIETNVKGTQVMLDASRKYGIEKFVHIST
HHHHHCCCCCCCEEEEHHHHHCCCCCCCCCCCEECCCCCEEEEEECHHHCCCHHEEEECH
DEVYGELGKEGQFTEESPLRPNSPYSVSKAAADMLARAYHRTYGLPVIVARPCNNYGPWQ
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
YPEKLIPVVIKKALNNEPIPVYGQGLNVREWLYVDDCIEAVYLLLQKGKPGEAYNIGSGE
CHHHHHHHHHHHHHCCCCCEEEECCCCHHHEEEHHHHHHHHHHHHHCCCCCCEEECCCCC
EKGNIEVVKEILRILGKPESLITFVEDRPGHDFRYSLNSKKIKMNYAWKHKVNFNEGIRF
CCCCHHHHHHHHHHHCCCHHHEEEECCCCCCCEEEECCCCEEEEEEEEEEECCCCCCHHH
VIDWYKKHF
HHHHHHHCC
>Mature Secondary Structure
MKILVTGGAGFIGSAFVRKYAYDHELIIVDKLTYAGDLRRIEEVRDRIKFYKADVADKTA
CEEEEECCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IEEIFDKEKPEAVVHFAAESHVDRSIQDPTPFIETNVKGTQVMLDASRKYGIEKFVHIST
HHHHHCCCCCCCEEEEHHHHHCCCCCCCCCCCEECCCCCEEEEEECHHHCCCHHEEEECH
DEVYGELGKEGQFTEESPLRPNSPYSVSKAAADMLARAYHRTYGLPVIVARPCNNYGPWQ
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
YPEKLIPVVIKKALNNEPIPVYGQGLNVREWLYVDDCIEAVYLLLQKGKPGEAYNIGSGE
CHHHHHHHHHHHHHCCCCCEEEECCCCHHHEEEHHHHHHHHHHHHHCCCCCCEEECCCCC
EKGNIEVVKEILRILGKPESLITFVEDRPGHDFRYSLNSKKIKMNYAWKHKVNFNEGIRF
CCCCHHHHHHHHHHHCCCHHHEEEECCCCCCCEEEECCCCEEEEEEEEEEECCCCCCHHH
VIDWYKKHF
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]