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The map label for this gene is rfbD [H]

Identifier: 78044837

GI number: 78044837

Start: 865124

End: 865975

Strand: Direct

Name: rfbD [H]

Synonym: CHY_0978

Alternate gene names: 78044837

Gene position: 865124-865975 (Clockwise)

Preceding gene: 78043500

Following gene: 78043771

Centisome position: 36.02

GC content: 38.15

Gene sequence:

>852_bases
ATGAAAAAAATAGTGATAGCCGGAGCCGGAGGACAATTAGGAAAAGCCTTTACCAAGAAGCTACAATCCTTAGGTATAGA
ATGCTTACCCCTTACCCGGAAAGAATTAGATATCACTGATTTACACCTGCTAAAAGAGCTCTTTTTAAACTTTCGTCCTC
AGTACTTAATAAACTGTGCCGCCTATAACGAAGTAGATAAAGCCGAAGAAGAAAAAGAAAAAGCACTTTTGGTAAATGGA
ATAGCATTAAGATACATAGGGAATTTAGCTGCCCAATACCAAACCACCGTGATCCATTACAGCACCGATTATGTTTTTAA
TGGCGAAAAAGAAGAGCCATACCTTATATCCGATCAGCCAAAGCCAATTAACCACTACGGGCGAACCAAACTTTTGGGAG
AAAAAGAAATCCTGACTCATAATCCTAAAACGTATGTAATTCGGGTTAGCTGGGTCTTTGGCGATGGCGAACAAAACTTT
ATTTATAAGCTTCTAAACTGGGCCAGCAAAAATGAAAAATTAAAAATAGTAGCAGACCAGGTTAGTAGTCCCACCTATAC
TGAAGAAATCGTAAAAATAACCTTAGACCTAATTAAAACCGAACAGTATGGTTTATATCAACTGTCAGGGGAAGGCCAAG
CATCAAGGTACGACTGGGCAAAATTCATTTTAGATTACCTAAACTGGCAGGGAGAACTAATACCGGCAGCAAGTCATGAT
TTTCCTACCAAAGCCAAGAGACCTAGATATTCAAAACTAAGTAACCTTCCCTTAAAAAACCTGTTAGGTTATCTTCCCGA
AACCTGGCAGGAATCTACGGAAAAATATCTAAAAGAACTAAAAAAGATATAA

Upstream 100 bases:

>100_bases
AAGAGCAAGTCGATGAGGTGCGCTGGGAAAATAAAGAGAACTCTCCATTCATAATTGATTAAAAATAAACAGAATAAATC
AAATTAAAGGAAAGAAGAGC

Downstream 100 bases:

>100_bases
AAAAAATAAAAAACTAAATAAACTTGAAAGGAGTCAAAAATGAAGATCCTAGTAACCGGAGGAGCAGGCTTTATTGGCAG
TGCCTTTGTAAGAAAGTATG

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKKIVIAGAGGQLGKAFTKKLQSLGIECLPLTRKELDITDLHLLKELFLNFRPQYLINCAAYNEVDKAEEEKEKALLVNG
IALRYIGNLAAQYQTTVIHYSTDYVFNGEKEEPYLISDQPKPINHYGRTKLLGEKEILTHNPKTYVIRVSWVFGDGEQNF
IYKLLNWASKNEKLKIVADQVSSPTYTEEIVKITLDLIKTEQYGLYQLSGEGQASRYDWAKFILDYLNWQGELIPAASHD
FPTKAKRPRYSKLSNLPLKNLLGYLPETWQESTEKYLKELKKI

Sequences:

>Translated_283_residues
MKKIVIAGAGGQLGKAFTKKLQSLGIECLPLTRKELDITDLHLLKELFLNFRPQYLINCAAYNEVDKAEEEKEKALLVNG
IALRYIGNLAAQYQTTVIHYSTDYVFNGEKEEPYLISDQPKPINHYGRTKLLGEKEILTHNPKTYVIRVSWVFGDGEQNF
IYKLLNWASKNEKLKIVADQVSSPTYTEEIVKITLDLIKTEQYGLYQLSGEGQASRYDWAKFILDYLNWQGELIPAASHD
FPTKAKRPRYSKLSNLPLKNLLGYLPETWQESTEKYLKELKKI
>Mature_283_residues
MKKIVIAGAGGQLGKAFTKKLQSLGIECLPLTRKELDITDLHLLKELFLNFRPQYLINCAAYNEVDKAEEEKEKALLVNG
IALRYIGNLAAQYQTTVIHYSTDYVFNGEKEEPYLISDQPKPINHYGRTKLLGEKEILTHNPKTYVIRVSWVFGDGEQNF
IYKLLNWASKNEKLKIVADQVSSPTYTEEIVKITLDLIKTEQYGLYQLSGEGQASRYDWAKFILDYLNWQGELIPAASHD
FPTKAKRPRYSKLSNLPLKNLLGYLPETWQESTEKYLKELKKI

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=278, Percent_Identity=25.1798561151079, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI11034825, Length=278, Percent_Identity=25.1798561151079, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI1788352, Length=294, Percent_Identity=31.9727891156463, Blast_Score=147, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32557; Mature: 32557

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVIAGAGGQLGKAFTKKLQSLGIECLPLTRKELDITDLHLLKELFLNFRPQYLINCA
CCEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHEEEEH
AYNEVDKAEEEKEKALLVNGIALRYIGNLAAQYQTTVIHYSTDYVFNGEKEEPYLISDQP
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEECCCCCCCEEECCCC
KPINHYGRTKLLGEKEILTHNPKTYVIRVSWVFGDGEQNFIYKLLNWASKNEKLKIVADQ
CCCCCCCCEEECCCCHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEC
VSSPTYTEEIVKITLDLIKTEQYGLYQLSGEGQASRYDWAKFILDYLNWQGELIPAASHD
CCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCCC
FPTKAKRPRYSKLSNLPLKNLLGYLPETWQESTEKYLKELKKI
CCCCCCCCCHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIVIAGAGGQLGKAFTKKLQSLGIECLPLTRKELDITDLHLLKELFLNFRPQYLINCA
CCEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHEEEEH
AYNEVDKAEEEKEKALLVNGIALRYIGNLAAQYQTTVIHYSTDYVFNGEKEEPYLISDQP
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEECCCCCCCEEECCCC
KPINHYGRTKLLGEKEILTHNPKTYVIRVSWVFGDGEQNFIYKLLNWASKNEKLKIVADQ
CCCCCCCCEEECCCCHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEC
VSSPTYTEEIVKITLDLIKTEQYGLYQLSGEGQASRYDWAKFILDYLNWQGELIPAASHD
CCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCCC
FPTKAKRPRYSKLSNLPLKNLLGYLPETWQESTEKYLKELKKI
CCCCCCCCCHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]