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The map label for this gene is pyk [H]

Identifier: 78043581

GI number: 78043581

Start: 1018022

End: 1019773

Strand: Direct

Name: pyk [H]

Synonym: CHY_1144

Alternate gene names: 78043581

Gene position: 1018022-1019773 (Clockwise)

Preceding gene: 78042620

Following gene: 78042716

Centisome position: 42.39

GC content: 45.15

Gene sequence:

>1752_bases
ATGAAACGCACCAAGATTGTTTGTACAATTGGGCCGGCTTCAAACGACGTAGGTATCTTAAAAGAAATGATTATATCCGG
AATGAATGTGGCCAGAATTAATTTTGCTCATGGCAGTCACGAGGAGCACCGGGAGAGAATTGAAAAGGTTAGACGGGCGT
CTTTAGAAGTAGGAATCCCGGTGGCAATTTTAATTGATACAAAAGGTCCGGAAATTCGCATTGGTAAAGTTGAAAACGGA
AAAATTGTCTTAAAAGAAGGGGACTTAGTGGTATTTGATCCGGATATAGCAGAGGGTCAAGGTTTGAGAGTTCCAGTAAA
TTATCCGGGATTAGCCCGGGATGTTAATGTCGGAGGAACTATTCTTTTAGATGATGGGTTAATAGAATTGAAAATCGAAG
ATATTCAGGGTAATAAAGTTATTGCCCGGGTTATAACCGGTGGTGAGTTATCCAATAACAAAGGAGTAAATTTACCGGGA
GTTAAAGTTAATCTTCCGGCGTTAACCGAAAAGGACCGGAAGGATATTGATTTTGGTATTGAAATTGGAGCGGACTTTAT
TGCCCACTCTTTTGTCCGAAAAGCAGCGGATGTGTTAGCTTTACGGCGTTACCTGGAAGAAAAAGGGGCGGATATGGAAA
TAATTGCTAAAATCGAAAACCAAGAAGGGGTCGAAAACATCGATGAAATCATCAAAGTTGCGGATGGGATAATGGTTGCC
CGGGGGGATCTGGGAGTAGAAATTCCAACGGAAGATGTGCCTTTGGTGCAAAAGGAAATAATTGAAAAGTGTAATAAAAA
TGGTAAACCGGTAATCACCGCCACCCAGATGTTAGATTCAATGATTAGAAATAAACGTCCGACCCGGGCCGAAGCTACCG
ATGTGGCTAATGCTATTTTTGACGGCACCGATGCGGTAATGCTGTCGGGAGAAACGGCTGCCGGGAAATATCCGGTAGAA
GCGGTTAAAACAATGGCCCGGATTGCGGAAAAAGCGGAAGAAAAATTACTCACGTTACGAAAACTAAATAAACCCACCAC
AAAATCTTTTAAAACTGTAACCGATGCCATAAGCCACGCCAGTGTTACCACCGCTGAAGAATTGGATGCGGGGGCAATTA
TCACGCCTACTTCTTCGGGCTATACGGCGCGAATGGTTTCCCGGTACCGCCCGGCGGTTCCTATTATTGCTGCCACACCG
GATATGAAGGTACTTAGAAAGTTGACGCTGGTTTGGGGGGTATTTCCCCTTTTGGTTAAAACCTCCGATTCTACCGATGA
AATGTTAAGTAAAGCAATAGAAGCCTCTTTAGAAAGTGGGCTTTTAAAACCTGGCGATTTGGTGGTGCTTACCGCCGGAG
TTCCGGTGGGCGTAAAAGGGACAACCAACCTTTTAAAAGTCCATGTAGCGGGAAATGTTTTGGCCAAAGGTGTGGGCATT
GGAACAAGGCCTATAACCGGAACGGCTCGGGTCTGTCGAACCGCCAGGGAAGCAATCGAGCGGATTCAGCCGGGAGATAT
TTTGGTAACCGAAGCAACCGACAGAGATTTTGTTTCGGCTATTGAAAAGGCCGCGGGACTGGTAGTTGAAGCGGGAGGAC
TTACGTCGCATGCGGCTATTGCGGGGTTAGAGTTTGGGATACCTGTAATAGTTGGGGTCGATGGTGCTACTTCGATAATT
AAAGATGGGGAAAAGTTAACTCTAGATCCCCAGAGGGGTCTGGTATACCACGGTTCTGCTAAAGTTTTATAA

Upstream 100 bases:

>100_bases
AAGCCCCTGGAAACTGCTTTTTCGGGGAAAAAGCAAATTGACCTTGAATATTATCGTTTAGCCGATATTTTATCGATTTA
ATACCGGGGTGAGAATTAAA

Downstream 100 bases:

>100_bases
AAAATGTTTATAGGGGGAGAAGGTAATGCCGGTATTGCGTAATTATACCGAAGAAGTGGTGGATGAAATACTTCAGGAAA
TTTTGCCCACCCAAGACGTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MKRTKIVCTIGPASNDVGILKEMIISGMNVARINFAHGSHEEHRERIEKVRRASLEVGIPVAILIDTKGPEIRIGKVENG
KIVLKEGDLVVFDPDIAEGQGLRVPVNYPGLARDVNVGGTILLDDGLIELKIEDIQGNKVIARVITGGELSNNKGVNLPG
VKVNLPALTEKDRKDIDFGIEIGADFIAHSFVRKAADVLALRRYLEEKGADMEIIAKIENQEGVENIDEIIKVADGIMVA
RGDLGVEIPTEDVPLVQKEIIEKCNKNGKPVITATQMLDSMIRNKRPTRAEATDVANAIFDGTDAVMLSGETAAGKYPVE
AVKTMARIAEKAEEKLLTLRKLNKPTTKSFKTVTDAISHASVTTAEELDAGAIITPTSSGYTARMVSRYRPAVPIIAATP
DMKVLRKLTLVWGVFPLLVKTSDSTDEMLSKAIEASLESGLLKPGDLVVLTAGVPVGVKGTTNLLKVHVAGNVLAKGVGI
GTRPITGTARVCRTAREAIERIQPGDILVTEATDRDFVSAIEKAAGLVVEAGGLTSHAAIAGLEFGIPVIVGVDGATSII
KDGEKLTLDPQRGLVYHGSAKVL

Sequences:

>Translated_583_residues
MKRTKIVCTIGPASNDVGILKEMIISGMNVARINFAHGSHEEHRERIEKVRRASLEVGIPVAILIDTKGPEIRIGKVENG
KIVLKEGDLVVFDPDIAEGQGLRVPVNYPGLARDVNVGGTILLDDGLIELKIEDIQGNKVIARVITGGELSNNKGVNLPG
VKVNLPALTEKDRKDIDFGIEIGADFIAHSFVRKAADVLALRRYLEEKGADMEIIAKIENQEGVENIDEIIKVADGIMVA
RGDLGVEIPTEDVPLVQKEIIEKCNKNGKPVITATQMLDSMIRNKRPTRAEATDVANAIFDGTDAVMLSGETAAGKYPVE
AVKTMARIAEKAEEKLLTLRKLNKPTTKSFKTVTDAISHASVTTAEELDAGAIITPTSSGYTARMVSRYRPAVPIIAATP
DMKVLRKLTLVWGVFPLLVKTSDSTDEMLSKAIEASLESGLLKPGDLVVLTAGVPVGVKGTTNLLKVHVAGNVLAKGVGI
GTRPITGTARVCRTAREAIERIQPGDILVTEATDRDFVSAIEKAAGLVVEAGGLTSHAAIAGLEFGIPVIVGVDGATSII
KDGEKLTLDPQRGLVYHGSAKVL
>Mature_583_residues
MKRTKIVCTIGPASNDVGILKEMIISGMNVARINFAHGSHEEHRERIEKVRRASLEVGIPVAILIDTKGPEIRIGKVENG
KIVLKEGDLVVFDPDIAEGQGLRVPVNYPGLARDVNVGGTILLDDGLIELKIEDIQGNKVIARVITGGELSNNKGVNLPG
VKVNLPALTEKDRKDIDFGIEIGADFIAHSFVRKAADVLALRRYLEEKGADMEIIAKIENQEGVENIDEIIKVADGIMVA
RGDLGVEIPTEDVPLVQKEIIEKCNKNGKPVITATQMLDSMIRNKRPTRAEATDVANAIFDGTDAVMLSGETAAGKYPVE
AVKTMARIAEKAEEKLLTLRKLNKPTTKSFKTVTDAISHASVTTAEELDAGAIITPTSSGYTARMVSRYRPAVPIIAATP
DMKVLRKLTLVWGVFPLLVKTSDSTDEMLSKAIEASLESGLLKPGDLVVLTAGVPVGVKGTTNLLKVHVAGNVLAKGVGI
GTRPITGTARVCRTAREAIERIQPGDILVTEATDRDFVSAIEKAAGLVVEAGGLTSHAAIAGLEFGIPVIVGVDGATSII
KDGEKLTLDPQRGLVYHGSAKVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=487, Percent_Identity=46.8172484599589, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI10835121, Length=487, Percent_Identity=46.8172484599589, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=45.1745379876797, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=45.1745379876797, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=44.7852760736196, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=48.0836236933798, Blast_Score=252, Evalue=6e-67,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=48.0836236933798, Blast_Score=252, Evalue=6e-67,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=45.1063829787234, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=45.1063829787234, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=43.4579439252336, Blast_Score=166, Evalue=8e-41,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=51.271186440678, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1788160, Length=473, Percent_Identity=42.4947145877378, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1787994, Length=117, Percent_Identity=40.1709401709402, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17544584, Length=492, Percent_Identity=44.9186991869919, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=45.979381443299, Blast_Score=370, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=45.979381443299, Blast_Score=370, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=45.979381443299, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=45.979381443299, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17564524, Length=127, Percent_Identity=31.496062992126, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319279, Length=481, Percent_Identity=42.8274428274428, Blast_Score=358, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=40.041067761807, Blast_Score=334, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24648964, Length=484, Percent_Identity=44.4214876033058, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI28571814, Length=484, Percent_Identity=44.4214876033058, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24648966, Length=445, Percent_Identity=46.0674157303371, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24581235, Length=480, Percent_Identity=37.2916666666667, Blast_Score=288, Evalue=9e-78,
Organism=Drosophila melanogaster, GI24646914, Length=305, Percent_Identity=43.2786885245902, Blast_Score=213, Evalue=3e-55,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62160; Mature: 62160

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTKIVCTIGPASNDVGILKEMIISGMNVARINFAHGSHEEHRERIEKVRRASLEVGIP
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
VAILIDTKGPEIRIGKVENGKIVLKEGDLVVFDPDIAEGQGLRVPVNYPGLARDVNVGGT
EEEEEECCCCEEEEEEECCCEEEEEECCEEEECCCCCCCCCCEEEECCCCCCEECCCCCE
ILLDDGLIELKIEDIQGNKVIARVITGGELSNNKGVNLPGVKVNLPALTEKDRKDIDFGI
EEEECCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEE
EIGADFIAHSFVRKAADVLALRRYLEEKGADMEIIAKIENQEGVENIDEIIKVADGIMVA
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEE
RGDLGVEIPTEDVPLVQKEIIEKCNKNGKPVITATQMLDSMIRNKRPTRAEATDVANAIF
ECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHH
DGTDAVMLSGETAAGKYPVEAVKTMARIAEKAEEKLLTLRKLNKPTTKSFKTVTDAISHA
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
SVTTAEELDAGAIITPTSSGYTARMVSRYRPAVPIIAATPDMKVLRKLTLVWGVFPLLVK
CCCCHHHCCCCEEEECCCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHEEE
TSDSTDEMLSKAIEASLESGLLKPGDLVVLTAGVPVGVKGTTNLLKVHVAGNVLAKGVGI
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEEEEECCHHHHCCCC
GTRPITGTARVCRTAREAIERIQPGDILVTEATDRDFVSAIEKAAGLVVEAGGLTSHAAI
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCEEEECCCCCCHHHH
AGLEFGIPVIVGVDGATSIIKDGEKLTLDPQRGLVYHGSAKVL
HHHCCCCEEEEECCCHHHHHCCCCEEEECCCCCEEEECCCCCC
>Mature Secondary Structure
MKRTKIVCTIGPASNDVGILKEMIISGMNVARINFAHGSHEEHRERIEKVRRASLEVGIP
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
VAILIDTKGPEIRIGKVENGKIVLKEGDLVVFDPDIAEGQGLRVPVNYPGLARDVNVGGT
EEEEEECCCCEEEEEEECCCEEEEEECCEEEECCCCCCCCCCEEEECCCCCCEECCCCCE
ILLDDGLIELKIEDIQGNKVIARVITGGELSNNKGVNLPGVKVNLPALTEKDRKDIDFGI
EEEECCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEE
EIGADFIAHSFVRKAADVLALRRYLEEKGADMEIIAKIENQEGVENIDEIIKVADGIMVA
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEE
RGDLGVEIPTEDVPLVQKEIIEKCNKNGKPVITATQMLDSMIRNKRPTRAEATDVANAIF
ECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHH
DGTDAVMLSGETAAGKYPVEAVKTMARIAEKAEEKLLTLRKLNKPTTKSFKTVTDAISHA
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
SVTTAEELDAGAIITPTSSGYTARMVSRYRPAVPIIAATPDMKVLRKLTLVWGVFPLLVK
CCCCHHHCCCCEEEECCCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHEEE
TSDSTDEMLSKAIEASLESGLLKPGDLVVLTAGVPVGVKGTTNLLKVHVAGNVLAKGVGI
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEEEEECCHHHHCCCC
GTRPITGTARVCRTAREAIERIQPGDILVTEATDRDFVSAIEKAAGLVVEAGGLTSHAAI
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCEEEECCCCCCHHHH
AGLEFGIPVIVGVDGATSIIKDGEKLTLDPQRGLVYHGSAKVL
HHHCCCCEEEEECCCHHHHHCCCCEEEECCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7549104 [H]