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The map label for this gene is pfkA [H]

Identifier: 78042620

GI number: 78042620

Start: 1017037

End: 1018002

Strand: Direct

Name: pfkA [H]

Synonym: CHY_1143

Alternate gene names: 78042620

Gene position: 1017037-1018002 (Clockwise)

Preceding gene: 78044709

Following gene: 78043581

Centisome position: 42.35

GC content: 43.48

Gene sequence:

>966_bases
ATGAACTCCATTGGGGTTTTAACCAGCGGTGGCGATGCGCCAGGAATGAACGCAGCGGTTCGGGCGGTAGTTAGAAAAGC
CCTTTTTCACGGGATAAAAGTTTACGGAATTGAACGAGGTTATGCCGGCTTAATTAATAATGAGATAAAAGAAATGAGTT
TGGGCAGTGTTGGTGACATCATCCAAAGGGGCGGAACGGTCTTAAAAACTGCCCGCTCTTTAGAATTTAAAACTTATGAA
GGCAGGTTAAGAGCTTTTCAAAATTTAAAAAACCATGGCATCGAAGGTTTGGTGGTTATTGGAGGAGATGGCTCCTTCCA
GGGAGCCTTAAAACTTTATGAGGAATTTGGGGTAAAGGTGGTCGGTATACCCGCAACTATTGACAATGACATTTATGGTA
CCGATTATTCAATTGGTTTTGATACGACGGTAAATACGGTGGTAGATGCTATTAATAAAATACGGGATACTGCCTTTTCC
CATGAACGTACCTTCATTGTTGAGGTTATGGGAAGAAACGCCGGGTTTATAGCTTTAGAAGCAGGAATTGCTGGAGGAGC
TGAATCGATAATTATCCCGGAAATACCTTACTCTATCGAGGAAATTTGCCAAAAGTTAATTAAGAGCCATCAGCGGGGAA
AATTACATAGCATAATTATCATTGCTGAGGGCGCGGGAAGCGGCATTAAGCTGGGAGAAGTAATTAAGGAAAAGACCGGT
TTTGAGACCCGGGTAACGATTTTAGGCCACATCCAGCGGGGTGGAAGCCCTTCGGCTTTTGACCGAATTTTAGCAAGCCG
GATGGGTGCTATGGCGGTTGATGCCTTAAGAGAGGGGATTTCCCAAGGGGTGATGGTTGCCTTTGAGAAGGGCGAATATG
TTTTAAAGCCCCTGGAAACTGCTTTTTCGGGGAAAAAGCAAATTGACCTTGAATATTATCGTTTAGCCGATATTTTATCG
ATTTAA

Upstream 100 bases:

>100_bases
TGCAAATCTTAAAAGAAACTCCCGTAGAGGAGCTGTTAAGAAGACGGTATCAGCGGTATCGCTATATAGGGAGCGGCATT
GTAGAGGGAGGCGTAAGCTA

Downstream 100 bases:

>100_bases
TACCGGGGTGAGAATTAAAATGAAACGCACCAAGATTGTTTGTACAATTGGGCCGGCTTCAAACGACGTAGGTATCTTAA
AAGAAATGATTATATCCGGA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNSIGVLTSGGDAPGMNAAVRAVVRKALFHGIKVYGIERGYAGLINNEIKEMSLGSVGDIIQRGGTVLKTARSLEFKTYE
GRLRAFQNLKNHGIEGLVVIGGDGSFQGALKLYEEFGVKVVGIPATIDNDIYGTDYSIGFDTTVNTVVDAINKIRDTAFS
HERTFIVEVMGRNAGFIALEAGIAGGAESIIIPEIPYSIEEICQKLIKSHQRGKLHSIIIIAEGAGSGIKLGEVIKEKTG
FETRVTILGHIQRGGSPSAFDRILASRMGAMAVDALREGISQGVMVAFEKGEYVLKPLETAFSGKKQIDLEYYRLADILS
I

Sequences:

>Translated_321_residues
MNSIGVLTSGGDAPGMNAAVRAVVRKALFHGIKVYGIERGYAGLINNEIKEMSLGSVGDIIQRGGTVLKTARSLEFKTYE
GRLRAFQNLKNHGIEGLVVIGGDGSFQGALKLYEEFGVKVVGIPATIDNDIYGTDYSIGFDTTVNTVVDAINKIRDTAFS
HERTFIVEVMGRNAGFIALEAGIAGGAESIIIPEIPYSIEEICQKLIKSHQRGKLHSIIIIAEGAGSGIKLGEVIKEKTG
FETRVTILGHIQRGGSPSAFDRILASRMGAMAVDALREGISQGVMVAFEKGEYVLKPLETAFSGKKQIDLEYYRLADILS
I
>Mature_321_residues
MNSIGVLTSGGDAPGMNAAVRAVVRKALFHGIKVYGIERGYAGLINNEIKEMSLGSVGDIIQRGGTVLKTARSLEFKTYE
GRLRAFQNLKNHGIEGLVVIGGDGSFQGALKLYEEFGVKVVGIPATIDNDIYGTDYSIGFDTTVNTVVDAINKIRDTAFS
HERTFIVEVMGRNAGFIALEAGIAGGAESIIIPEIPYSIEEICQKLIKSHQRGKLHSIIIIAEGAGSGIKLGEVIKEKTG
FETRVTILGHIQRGGSPSAFDRILASRMGAMAVDALREGISQGVMVAFEKGEYVLKPLETAFSGKKQIDLEYYRLADILS
I

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=332, Percent_Identity=44.2771084337349, Blast_Score=251, Evalue=4e-67,
Organism=Homo sapiens, GI266453619, Length=332, Percent_Identity=43.0722891566265, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI266453768, Length=332, Percent_Identity=43.0722891566265, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI266453748, Length=332, Percent_Identity=43.0722891566265, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI4505749, Length=332, Percent_Identity=43.0722891566265, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI48762920, Length=342, Percent_Identity=42.9824561403509, Blast_Score=245, Evalue=3e-65,
Organism=Escherichia coli, GI1790350, Length=279, Percent_Identity=52.6881720430108, Blast_Score=283, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI25147584, Length=332, Percent_Identity=43.0722891566265, Blast_Score=248, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=40.2597402597403, Blast_Score=210, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI72003601, Length=289, Percent_Identity=33.5640138408304, Blast_Score=168, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6323861, Length=334, Percent_Identity=39.2215568862275, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6321679, Length=313, Percent_Identity=41.2140575079872, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI28573326, Length=331, Percent_Identity=44.1087613293051, Blast_Score=255, Evalue=2e-68,
Organism=Drosophila melanogaster, GI17647809, Length=331, Percent_Identity=43.202416918429, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24652337, Length=331, Percent_Identity=43.202416918429, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34511; Mature: 34511

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIGVLTSGGDAPGMNAAVRAVVRKALFHGIKVYGIERGYAGLINNEIKEMSLGSVGDI
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCHHHH
IQRGGTVLKTARSLEFKTYEGRLRAFQNLKNHGIEGLVVIGGDGSFQGALKLYEEFGVKV
HHCCCHHHHHHHHCEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCEE
VGIPATIDNDIYGTDYSIGFDTTVNTVVDAINKIRDTAFSHERTFIVEVMGRNAGFIALE
EEECEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEE
AGIAGGAESIIIPEIPYSIEEICQKLIKSHQRGKLHSIIIIAEGAGSGIKLGEVIKEKTG
CCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHCC
FETRVTILGHIQRGGSPSAFDRILASRMGAMAVDALREGISQGVMVAFEKGEYVLKPLET
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEHHHHH
AFSGKKQIDLEYYRLADILSI
HHCCCCEECHHHHHHHHHHCC
>Mature Secondary Structure
MNSIGVLTSGGDAPGMNAAVRAVVRKALFHGIKVYGIERGYAGLINNEIKEMSLGSVGDI
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCHHHH
IQRGGTVLKTARSLEFKTYEGRLRAFQNLKNHGIEGLVVIGGDGSFQGALKLYEEFGVKV
HHCCCHHHHHHHHCEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCEE
VGIPATIDNDIYGTDYSIGFDTTVNTVVDAINKIRDTAFSHERTFIVEVMGRNAGFIALE
EEECEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEE
AGIAGGAESIIIPEIPYSIEEICQKLIKSHQRGKLHSIIIIAEGAGSGIKLGEVIKEKTG
CCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHCC
FETRVTILGHIQRGGSPSAFDRILASRMGAMAVDALREGISQGVMVAFEKGEYVLKPLET
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEHHHHH
AFSGKKQIDLEYYRLADILSI
HHCCCCEECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA