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The map label for this gene is siaT [H]

Identifier: 77465453

GI number: 77465453

Start: 521046

End: 522347

Strand: Reverse

Name: siaT [H]

Synonym: RSP_3451

Alternate gene names: 77465453

Gene position: 522347-521046 (Counterclockwise)

Preceding gene: 77465454

Following gene: 77465452

Centisome position: 55.39

GC content: 66.97

Gene sequence:

>1302_bases
ATGGGAAAGATCATTGCCACCCTGCTCGGCCTTCTCACGCTGCTTCTGGGCAGCGGCCTCTGGATCGGCCTCGGGCTCAT
CGGCACCGGCGTGGGGCTGCTTACGCTCTTCCGGCCGAACATGCCGGGGCTCAAGCTTCTCGCGCAGCAGACCTACAACG
TCGTCGTCTCGCCCGAGCTGCTCGCGCTGCCGCTCTTCATCCTGATGGCCGAGATCCTCTTCCGCACGAAGATCTCCGAG
GCGCTGTTCACCGGCCTCTCGCCCTGGCTGCGCCGGGTGCCGGGGCGGCTGTCGCACCTGACCGTGCTCGGCTGCACCAT
GTTCGCCTCGGTCTGCGGCTCGTCGGCGGCGACCACGGCCACCGTGGGGCGCATCACCGCCAAGGAACTGATCGACCGCG
GCTACAACCGCGACCTCGTGACGGGCAGCCTTGCCGGGGCGGGGACGCTCGGCTTCCTGATCCCGCCCTCGACCATCATG
ATCATCTACGGGGTGATGGCCGAGGAATCGATCCTGCGGCTCTTCATCGCCGGCATCGTGCCGGGGCTGATGCTCGCGGC
CTCCTACATGGGGTATCTCGCGATCCGCTCGTGGCTCAACCCCGCGCTCGTGGGCGCGACTCCCCCGCGCACCAGCTTCC
GCGAGAAGATGGTGGCGTTGAAGGATCTGGGGCCGCTTCTTCTCCTCATCGCGGGCGTCATCGGCTCGATGTACGGCGGG
ATCGCCTCGCCGACCGAGGCCGCCACCGTGGGCGTGGTCGTCTCGATGATCATCGGCTTCGCGCAGCGCACGCTGACGCT
GGGGGGCCTTCTGAGCGCCGCGCGTCAGGCGGCCGAAAGCTGCGCCATGATCGGCCTCATCATGATCGGGGCGATGTTCC
TGTCGACGATGATCGGCTATCTCGGCGTGCCGCGCTACATCGCGACCGAGATCCAGAGCTGGGGCATGTCGCCCCTGATG
CTCGTGGTGGTGCTGCTGATCTTCTACGTCCTGCTCGGCACGGTGATGGAGGGGCTCGGCATCATCGTGATGACGCTGCC
GATCACGCTGCCGCTGGCGGTGGCCGCGGGCTACGACAAGGTCTGGTTCGGCATCTTCCTCGTGATCGTGGTCGAGATGG
CCCAGATCACGCCGCCGGTGGGCTTCAACCTCACGGTGATCCAGCGGCTGACGGGGGACAGCATGGGGCGCATCACGCGC
GCCACCATGCCCTTCTTCCTCATCATGGCGGCCTTCGTGCTGCTCATCGCGGTCTTCCCGGGCATCATCGGCTTCCTGCC
GGACCTGATACGCGGCAACTGA

Upstream 100 bases:

>100_bases
GGGCGCCACGATCCTCACCCTGCAGTGCCTCGCCCAGATCCTGCGCCTCTTGCGCGGCGAGGAACTCTCCGTCGGCCCCG
GTCTGGAATAGGAGCCGTCC

Downstream 100 bases:

>100_bases
CAGAGGTCCGCAGCCCTGACGGCTGCGGATCATCCCGAAATGAGTATGATCCCGTGACGATGACCCCCAGCCTTCTTCCC
TGCGGCGACAGCGCCCTCAC

Product: TRAP-T family transporter large inner membrane subunit

Products: NA

Alternate protein names: N-acetylneuraminic acid permease; N-acetylneuraminic acid transporter; Neu5Ac permease [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MGKIIATLLGLLTLLLGSGLWIGLGLIGTGVGLLTLFRPNMPGLKLLAQQTYNVVVSPELLALPLFILMAEILFRTKISE
ALFTGLSPWLRRVPGRLSHLTVLGCTMFASVCGSSAATTATVGRITAKELIDRGYNRDLVTGSLAGAGTLGFLIPPSTIM
IIYGVMAEESILRLFIAGIVPGLMLAASYMGYLAIRSWLNPALVGATPPRTSFREKMVALKDLGPLLLLIAGVIGSMYGG
IASPTEAATVGVVVSMIIGFAQRTLTLGGLLSAARQAAESCAMIGLIMIGAMFLSTMIGYLGVPRYIATEIQSWGMSPLM
LVVVLLIFYVLLGTVMEGLGIIVMTLPITLPLAVAAGYDKVWFGIFLVIVVEMAQITPPVGFNLTVIQRLTGDSMGRITR
ATMPFFLIMAAFVLLIAVFPGIIGFLPDLIRGN

Sequences:

>Translated_433_residues
MGKIIATLLGLLTLLLGSGLWIGLGLIGTGVGLLTLFRPNMPGLKLLAQQTYNVVVSPELLALPLFILMAEILFRTKISE
ALFTGLSPWLRRVPGRLSHLTVLGCTMFASVCGSSAATTATVGRITAKELIDRGYNRDLVTGSLAGAGTLGFLIPPSTIM
IIYGVMAEESILRLFIAGIVPGLMLAASYMGYLAIRSWLNPALVGATPPRTSFREKMVALKDLGPLLLLIAGVIGSMYGG
IASPTEAATVGVVVSMIIGFAQRTLTLGGLLSAARQAAESCAMIGLIMIGAMFLSTMIGYLGVPRYIATEIQSWGMSPLM
LVVVLLIFYVLLGTVMEGLGIIVMTLPITLPLAVAAGYDKVWFGIFLVIVVEMAQITPPVGFNLTVIQRLTGDSMGRITR
ATMPFFLIMAAFVLLIAVFPGIIGFLPDLIRGN
>Mature_432_residues
GKIIATLLGLLTLLLGSGLWIGLGLIGTGVGLLTLFRPNMPGLKLLAQQTYNVVVSPELLALPLFILMAEILFRTKISEA
LFTGLSPWLRRVPGRLSHLTVLGCTMFASVCGSSAATTATVGRITAKELIDRGYNRDLVTGSLAGAGTLGFLIPPSTIMI
IYGVMAEESILRLFIAGIVPGLMLAASYMGYLAIRSWLNPALVGATPPRTSFREKMVALKDLGPLLLLIAGVIGSMYGGI
ASPTEAATVGVVVSMIIGFAQRTLTLGGLLSAARQAAESCAMIGLIMIGAMFLSTMIGYLGVPRYIATEIQSWGMSPLML
VVVLLIFYVLLGTVMEGLGIIVMTLPITLPLAVAAGYDKVWFGIFLVIVVEMAQITPPVGFNLTVIQRLTGDSMGRITRA
TMPFFLIMAAFVLLIAVFPGIIGFLPDLIRGN

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system siaPT involved in the uptake of sialic acid [H]

COG id: COG1593

COG function: function code G; TRAP-type C4-dicarboxylate transport system, large permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the TRAP transporter large permease family [H]

Homologues:

Organism=Escherichia coli, GI48994950, Length=370, Percent_Identity=27.027027027027, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010656
- InterPro:   IPR007387
- InterPro:   IPR004681 [H]

Pfam domain/function: PF06808 DctM; PF04290 DctQ [H]

EC number: NA

Molecular weight: Translated: 45853; Mature: 45722

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIIATLLGLLTLLLGSGLWIGLGLIGTGVGLLTLFRPNMPGLKLLAQQTYNVVVSPEL
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCEEECCHH
LALPLFILMAEILFRTKISEALFTGLSPWLRRVPGRLSHLTVLGCTMFASVCGSSAATTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
TVGRITAKELIDRGYNRDLVTGSLAGAGTLGFLIPPSTIMIIYGVMAEESILRLFIAGIV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
PGLMLAASYMGYLAIRSWLNPALVGATPPRTSFREKMVALKDLGPLLLLIAGVIGSMYGG
HHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
IASPTEAATVGVVVSMIIGFAQRTLTLGGLLSAARQAAESCAMIGLIMIGAMFLSTMIGY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVPRYIATEIQSWGMSPLMLVVVLLIFYVLLGTVMEGLGIIVMTLPITLPLAVAAGYDK
HCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VWFGIFLVIVVEMAQITPPVGFNLTVIQRLTGDSMGRITRATMPFFLIMAAFVLLIAVFP
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIIGFLPDLIRGN
HHHHHHHHHHCCC
>Mature Secondary Structure 
GKIIATLLGLLTLLLGSGLWIGLGLIGTGVGLLTLFRPNMPGLKLLAQQTYNVVVSPEL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCEEECCHH
LALPLFILMAEILFRTKISEALFTGLSPWLRRVPGRLSHLTVLGCTMFASVCGSSAATTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
TVGRITAKELIDRGYNRDLVTGSLAGAGTLGFLIPPSTIMIIYGVMAEESILRLFIAGIV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHH
PGLMLAASYMGYLAIRSWLNPALVGATPPRTSFREKMVALKDLGPLLLLIAGVIGSMYGG
HHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
IASPTEAATVGVVVSMIIGFAQRTLTLGGLLSAARQAAESCAMIGLIMIGAMFLSTMIGY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVPRYIATEIQSWGMSPLMLVVVLLIFYVLLGTVMEGLGIIVMTLPITLPLAVAAGYDK
HCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VWFGIFLVIVVEMAQITPPVGFNLTVIQRLTGDSMGRITRATMPFFLIMAAFVLLIAVFP
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIIGFLPDLIRGN
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]