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The map label for this gene is kipI [H]

Identifier: 77465452

GI number: 77465452

Start: 520255

End: 520992

Strand: Reverse

Name: kipI [H]

Synonym: RSP_3450

Alternate gene names: 77465452

Gene position: 520992-520255 (Counterclockwise)

Preceding gene: 77465453

Following gene: 77465451

Centisome position: 55.25

GC content: 71.0

Gene sequence:

>738_bases
GTGACGATGACCCCCAGCCTTCTTCCCTGCGGCGACAGCGCCCTCACCGTCCAGATCGGCGAAAGCATCGATGCCGAGGC
CAATGCCCGCATCATCGCGCTTGCGGAGGCTGTCGAGGAGGCCGCGCGGTCCGGCGACCTCGCGGGCGTCCTCGACATCG
TGCCGACCTACCGCTCGCTTCTCGTGCGCTACGACCCCGAACGCATCCGCGCGAAGGACCTCGGCCCCCGGCTTCTCGAG
ATCGCGGCCCGGCCGCTCGCGGCGGCGGCGGGCCGGCTCTGGCGGATCCCCTGCCTCTACGGCGGCGAGGTGGGGCAGGA
TCTTCAGGAGATGGCCCGCATGAAGGGCCTCTCGCCCGAAGAGCTGATCGCACTCCATTCCGGGACCGACTACCGCGTCT
TCATGATCGGCTTCGCGCCGGGCTTCGCCTATCTCGGCGGCCAGCCCGAGATCCTGCACACGCCCCGCCTGCCGAAGCCG
CGCCAGAACATCCCGCGCGGCGCGGTCGGCATCGGCGGCCAGCAGGGCAACATCAATTCCGTCGCCGGGCCCTCGGGCTG
GCGCTTCCTCGGCTGGACGCCGGTGCGCAGCTTCGATCCCGCCCGCGCCGAGCCCTTCCTGTTCCGCGCAGGCGACCATA
TCCGCTTCCATCCTGTCGGACCCGAGGAGGCGCGCGCGCTCGAGGCGCGCAGTGCCGCAGGCGAGATGATCGTGACCCCC
GAAGAGATCCGCGCATGA

Upstream 100 bases:

>100_bases
TCCCGGGCATCATCGGCTTCCTGCCGGACCTGATACGCGGCAACTGACAGAGGTCCGCAGCCCTGACGGCTGCGGATCAT
CCCGAAATGAGTATGATCCC

Downstream 100 bases:

>100_bases
GCCTCGAGATCCTGACCGCAGGTCCCATGCTGACCGTGCAGGACGCGGGCCGCTTCGGCCTGCGCCACATGGGCGTCTCG
CCCGCGGGCCCCATCGACCG

Product: hypothetical protein

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTMTPSLLPCGDSALTVQIGESIDAEANARIIALAEAVEEAARSGDLAGVLDIVPTYRSLLVRYDPERIRAKDLGPRLLE
IAARPLAAAAGRLWRIPCLYGGEVGQDLQEMARMKGLSPEELIALHSGTDYRVFMIGFAPGFAYLGGQPEILHTPRLPKP
RQNIPRGAVGIGGQQGNINSVAGPSGWRFLGWTPVRSFDPARAEPFLFRAGDHIRFHPVGPEEARALEARSAAGEMIVTP
EEIRA

Sequences:

>Translated_245_residues
MTMTPSLLPCGDSALTVQIGESIDAEANARIIALAEAVEEAARSGDLAGVLDIVPTYRSLLVRYDPERIRAKDLGPRLLE
IAARPLAAAAGRLWRIPCLYGGEVGQDLQEMARMKGLSPEELIALHSGTDYRVFMIGFAPGFAYLGGQPEILHTPRLPKP
RQNIPRGAVGIGGQQGNINSVAGPSGWRFLGWTPVRSFDPARAEPFLFRAGDHIRFHPVGPEEARALEARSAAGEMIVTP
EEIRA
>Mature_244_residues
TMTPSLLPCGDSALTVQIGESIDAEANARIIALAEAVEEAARSGDLAGVLDIVPTYRSLLVRYDPERIRAKDLGPRLLEI
AARPLAAAAGRLWRIPCLYGGEVGQDLQEMARMKGLSPEELIALHSGTDYRVFMIGFAPGFAYLGGQPEILHTPRLPKPR
QNIPRGAVGIGGQQGNINSVAGPSGWRFLGWTPVRSFDPARAEPFLFRAGDHIRFHPVGPEEARALEARSAAGEMIVTPE
EIRA

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=210, Percent_Identity=38.0952380952381, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 26440; Mature: 26308

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTPSLLPCGDSALTVQIGESIDAEANARIIALAEAVEEAARSGDLAGVLDIVPTYRSL
CCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LVRYDPERIRAKDLGPRLLEIAARPLAAAAGRLWRIPCLYGGEVGQDLQEMARMKGLSPE
HEEECHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCEEECCCCCHHHHHHHHHCCCCHH
ELIALHSGTDYRVFMIGFAPGFAYLGGQPEILHTPRLPKPRQNIPRGAVGIGGQQGNINS
HEEEEECCCCEEEEEEEECCCHHEECCCCCEEECCCCCCCHHCCCCCCEECCCCCCCCCC
VAGPSGWRFLGWTPVRSFDPARAEPFLFRAGDHIRFHPVGPEEARALEARSAAGEMIVTP
CCCCCCCEEEECCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHCCCCEEECH
EEIRA
HHHCC
>Mature Secondary Structure 
TMTPSLLPCGDSALTVQIGESIDAEANARIIALAEAVEEAARSGDLAGVLDIVPTYRSL
CCCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LVRYDPERIRAKDLGPRLLEIAARPLAAAAGRLWRIPCLYGGEVGQDLQEMARMKGLSPE
HEEECHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCEEECCCCCHHHHHHHHHCCCCHH
ELIALHSGTDYRVFMIGFAPGFAYLGGQPEILHTPRLPKPRQNIPRGAVGIGGQQGNINS
HEEEEECCCCEEEEEEEECCCHHEECCCCCEEECCCCCCCHHCCCCCCEECCCCCCCCCC
VAGPSGWRFLGWTPVRSFDPARAEPFLFRAGDHIRFHPVGPEEARALEARSAAGEMIVTP
CCCCCCCEEEECCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHCCCCEEECH
EEIRA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]