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The map label for this gene is potB [H]

Identifier: 77465338

GI number: 77465338

Start: 399548

End: 400411

Strand: Reverse

Name: potB [H]

Synonym: RSP_3336

Alternate gene names: 77465338

Gene position: 400411-399548 (Counterclockwise)

Preceding gene: 77465339

Following gene: 77465337

Centisome position: 42.46

GC content: 62.15

Gene sequence:

>864_bases
ATGGAGCGCATTCTGAAATCGCCGAACGTGACGGGCTGGCTTCTGGCCGCGCCGCTGACGCTGGTGCTGGCGGCCTTCCT
CGTTCTGCCGATCGTGATGATCGTGATCGTGAGCTTCTGGACGGCCACCGAATTCTCGATCGTCCCGGCCTTCTCCTGGG
AGAATTACGAGTTCCTGTTCGGCTCGGAGGTCACCTACCGGGTGTTCCTCAATACCTTCAAATATGCCGCGATCACCTGG
GCGGTGACGCTGGTGCTGGGCTTCACCGTCGCCTATTTCCTCGCCTTCCATGTCCGCAGCCAGACCTGGCAGACGGCGCT
GTTCCTGCTCTGCACCATCCCGTTCTGGACCTCGAACATCATCCGCATGATCTCGTGGATCCCGTTCCTCGGTCGCAACG
GCATCGCCAATTCGACGCTGATCTCGTGGGGGGTGATCGACGCGCCGCTGGAGTGGCTGCTCTTCTCGGACTTCTCGGTG
ATCCTGGCCTTCGTGCATCTCTACACGCTGTTCATGGTGGTGCCGATCTTCAACACCATGATGCGGATCGACCGGAGCCT
CCTCGAGGCGGCCAGCGACGCCGGGGCCTCGGGCTTCCAGACGCTCTGGAACGTGATCCTGCCCCTGACCAAGCCCGGCA
TCATGATCGGCTCGATCTTCGTCATCACCCTCGTCATGGGCGACTTCATCACCGTGCGCTTCATGTCGGGCTCGCAGGCG
GCGAATGTGGGCCGGCTGATCTCGAACGACATCGCGCTGCTGCAATATCCCTCGGCTTCGGCCACGGCGGTGGTGCTGCT
CGCGACCGTGCTTCTCACCATCGGCATCCTGCTGCGCCTCGTCGATATCCGGAAGGAGCTCTGA

Upstream 100 bases:

>100_bases
AACAGCCGCGATCCGACCGGACCTGCCGCGCAGACCTCACTGGCCACCGGCCCCTGGCCTTTCCCGCGAGGGGAAGGGCC
GCCCGCCTCCCGGAGGAAAG

Downstream 100 bases:

>100_bases
GATGGAGCCGCGTCCGCGCTCGTTCTACATCCTCGCCGCCGTCTTCGGCCTGTTCCTCCTGTTCCTCTACGGGCCCACGA
TCACCATCGCGATCCTCTCC

Product: ABC spermidine/putrescine transporter, inner membrane subunit

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MERILKSPNVTGWLLAAPLTLVLAAFLVLPIVMIVIVSFWTATEFSIVPAFSWENYEFLFGSEVTYRVFLNTFKYAAITW
AVTLVLGFTVAYFLAFHVRSQTWQTALFLLCTIPFWTSNIIRMISWIPFLGRNGIANSTLISWGVIDAPLEWLLFSDFSV
ILAFVHLYTLFMVVPIFNTMMRIDRSLLEAASDAGASGFQTLWNVILPLTKPGIMIGSIFVITLVMGDFITVRFMSGSQA
ANVGRLISNDIALLQYPSASATAVVLLATVLLTIGILLRLVDIRKEL

Sequences:

>Translated_287_residues
MERILKSPNVTGWLLAAPLTLVLAAFLVLPIVMIVIVSFWTATEFSIVPAFSWENYEFLFGSEVTYRVFLNTFKYAAITW
AVTLVLGFTVAYFLAFHVRSQTWQTALFLLCTIPFWTSNIIRMISWIPFLGRNGIANSTLISWGVIDAPLEWLLFSDFSV
ILAFVHLYTLFMVVPIFNTMMRIDRSLLEAASDAGASGFQTLWNVILPLTKPGIMIGSIFVITLVMGDFITVRFMSGSQA
ANVGRLISNDIALLQYPSASATAVVLLATVLLTIGILLRLVDIRKEL
>Mature_287_residues
MERILKSPNVTGWLLAAPLTLVLAAFLVLPIVMIVIVSFWTATEFSIVPAFSWENYEFLFGSEVTYRVFLNTFKYAAITW
AVTLVLGFTVAYFLAFHVRSQTWQTALFLLCTIPFWTSNIIRMISWIPFLGRNGIANSTLISWGVIDAPLEWLLFSDFSV
ILAFVHLYTLFMVVPIFNTMMRIDRSLLEAASDAGASGFQTLWNVILPLTKPGIMIGSIFVITLVMGDFITVRFMSGSQA
ANVGRLISNDIALLQYPSASATAVVLLATVLLTIGILLRLVDIRKEL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=274, Percent_Identity=33.9416058394161, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787080, Length=236, Percent_Identity=33.8983050847458, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1787713, Length=258, Percent_Identity=27.906976744186, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32008; Mature: 32008

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERILKSPNVTGWLLAAPLTLVLAAFLVLPIVMIVIVSFWTATEFSIVPAFSWENYEFLF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEE
GSEVTYRVFLNTFKYAAITWAVTLVLGFTVAYFLAFHVRSQTWQTALFLLCTIPFWTSNI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IRMISWIPFLGRNGIANSTLISWGVIDAPLEWLLFSDFSVILAFVHLYTLFMVVPIFNTM
HHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRIDRSLLEAASDAGASGFQTLWNVILPLTKPGIMIGSIFVITLVMGDFITVRFMSGSQA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCH
ANVGRLISNDIALLQYPSASATAVVLLATVLLTIGILLRLVDIRKEL
HHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERILKSPNVTGWLLAAPLTLVLAAFLVLPIVMIVIVSFWTATEFSIVPAFSWENYEFLF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEE
GSEVTYRVFLNTFKYAAITWAVTLVLGFTVAYFLAFHVRSQTWQTALFLLCTIPFWTSNI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
IRMISWIPFLGRNGIANSTLISWGVIDAPLEWLLFSDFSVILAFVHLYTLFMVVPIFNTM
HHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRIDRSLLEAASDAGASGFQTLWNVILPLTKPGIMIGSIFVITLVMGDFITVRFMSGSQA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCH
ANVGRLISNDIALLQYPSASATAVVLLATVLLTIGILLRLVDIRKEL
HHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]