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The map label for this gene is acrA [H]

Identifier: 77465322

GI number: 77465322

Start: 381789

End: 382952

Strand: Reverse

Name: acrA [H]

Synonym: RSP_3321

Alternate gene names: 77465322

Gene position: 382952-381789 (Counterclockwise)

Preceding gene: 77465323

Following gene: 77465321

Centisome position: 40.61

GC content: 71.48

Gene sequence:

>1164_bases
GTGACCTTCCCCCGCCTTCCGCTCGTTCTTGCGGCCCTCATCCTCGCCGCCCCGGCCGTCCGTGCCGAAGGGCCTGCCGG
GGCAGAGCGTCCGCCGTCTCCGGTCACGGTGGTCACCCTGACCGCGAAGGACTATCCGATCATCTCGATCCTTCCGGGCC
GCGTGACGGCCTCGGAAGTGGCCGAGGTGCGGCCTCAGGTGAACGGCATCATCCGCGAGCGGCTGTTCGAGGAGGGGACC
GAGGTCGAGAAGGACCAGCCGCTCTACAAGATCGAGGACGACATGTATGCGGCCGCGGTCGCGGCGGCTCGCGCGGCCGT
CACGCAGGCCGAAGCCACGCTGCGCTCGGCCGAGAAGGACGCCCGCCGTGCCGAAGAGCTGTTCGGCAACAATGCGGGCA
GCGAGCAGCGGCTCGACACGGCCGTGGCCACCCGCGACGCGGCGGCGGCGGCGCTGCAGATGGCCCGGGCCGAGCTGAAG
ACGGCCGAGATCAACCTCAGCCGCACGACGGTGCGCGCGCCCATCGCGGGCACCATCGGCCTGTCGCAGACCACGACGGG
GGCGCTGGTGGCCGAAGGGCAGGAGGCGACGCTCACCACGATCCGCAGCCTCGACCGGGTGCATGTGGATGTGACGCAAT
CGGCGACCGACCTGCTGAAATGGCGCAGCCGGACGGGCAAGAACACCGTCGGACGCGTCGACGAGAATGTGGCGCTGATC
CTCGCCGACGGCAGCCGCTATCCGCTGCGCGGCCGCCTCGCCGCGGCCGAGCCGCAGGTGCAGCCCACCACGGGCATGGT
CACGCTGCGGATCCATTTCCCCAATCCGCAGAGGATCCTGCTGCCCGGCATGTATGTGCAGGTCGAGCTGCCGGTGGCGC
GCGCCGACAAGGCGGTGGCGGTGCCGCATCAGGCCGTCATCCGCGACCGGCTGGGGGTGCCCGCAGTCTGGCTCGTCACC
GAGCAGAATGTCATCGAGGCGCGCCCCGTCGAGATCGTGAAGTCCGACGGCGACCGCTGGATCGTGACCAAGGGGCTCGA
GACGGGCGACCGCGTGGTGGTTGCGGGCTTCCAGAAGGCGGCCGTGGGCGCGCCGGTGGCGCCCGAGGAACAGGGCGCGC
TGCCCGGGCCGGCCGTGAAGGCCGAAGCCGCCACCGCGAACTGA

Upstream 100 bases:

>100_bases
CTGAAAAGGCGGGCGTTCGCGGTCATCCTGCACGCAGGTGCTTGACTTTGGAAACTGGTTGGTTTCCTAAGCAGGCCACC
GCTGCCGATGGAGTCTGCCC

Downstream 100 bases:

>100_bases
AGGGCAGGGAGCCGCAATGGGACGTTTCTTCATCGACCGCCCGGTCTTCGCCTGGGTGATCGCCATCGTCATCATGGCGC
TCGGGGTCCTGTCGATCCTG

Product: cation/multidrug efflux pump, membrane-fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTFPRLPLVLAALILAAPAVRAEGPAGAERPPSPVTVVTLTAKDYPIISILPGRVTASEVAEVRPQVNGIIRERLFEEGT
EVEKDQPLYKIEDDMYAAAVAAARAAVTQAEATLRSAEKDARRAEELFGNNAGSEQRLDTAVATRDAAAAALQMARAELK
TAEINLSRTTVRAPIAGTIGLSQTTTGALVAEGQEATLTTIRSLDRVHVDVTQSATDLLKWRSRTGKNTVGRVDENVALI
LADGSRYPLRGRLAAAEPQVQPTTGMVTLRIHFPNPQRILLPGMYVQVELPVARADKAVAVPHQAVIRDRLGVPAVWLVT
EQNVIEARPVEIVKSDGDRWIVTKGLETGDRVVVAGFQKAAVGAPVAPEEQGALPGPAVKAEAATAN

Sequences:

>Translated_387_residues
MTFPRLPLVLAALILAAPAVRAEGPAGAERPPSPVTVVTLTAKDYPIISILPGRVTASEVAEVRPQVNGIIRERLFEEGT
EVEKDQPLYKIEDDMYAAAVAAARAAVTQAEATLRSAEKDARRAEELFGNNAGSEQRLDTAVATRDAAAAALQMARAELK
TAEINLSRTTVRAPIAGTIGLSQTTTGALVAEGQEATLTTIRSLDRVHVDVTQSATDLLKWRSRTGKNTVGRVDENVALI
LADGSRYPLRGRLAAAEPQVQPTTGMVTLRIHFPNPQRILLPGMYVQVELPVARADKAVAVPHQAVIRDRLGVPAVWLVT
EQNVIEARPVEIVKSDGDRWIVTKGLETGDRVVVAGFQKAAVGAPVAPEEQGALPGPAVKAEAATAN
>Mature_386_residues
TFPRLPLVLAALILAAPAVRAEGPAGAERPPSPVTVVTLTAKDYPIISILPGRVTASEVAEVRPQVNGIIRERLFEEGTE
VEKDQPLYKIEDDMYAAAVAAARAAVTQAEATLRSAEKDARRAEELFGNNAGSEQRLDTAVATRDAAAAALQMARAELKT
AEINLSRTTVRAPIAGTIGLSQTTTGALVAEGQEATLTTIRSLDRVHVDVTQSATDLLKWRSRTGKNTVGRVDENVALIL
ADGSRYPLRGRLAAAEPQVQPTTGMVTLRIHFPNPQRILLPGMYVQVELPVARADKAVAVPHQAVIRDRLGVPAVWLVTE
QNVIEARPVEIVKSDGDRWIVTKGLETGDRVVVAGFQKAAVGAPVAPEEQGALPGPAVKAEAATAN

Specific function: Probable periplasmic linker protein component of the sepABC efflux pump with unknown specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=366, Percent_Identity=38.2513661202186, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1786668, Length=348, Percent_Identity=40.2298850574713, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789929, Length=356, Percent_Identity=36.7977528089888, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI87082036, Length=366, Percent_Identity=25.4098360655738, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41124; Mature: 40993

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFPRLPLVLAALILAAPAVRAEGPAGAERPPSPVTVVTLTAKDYPIISILPGRVTASEV
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCHHHH
AEVRPQVNGIIRERLFEEGTEVEKDQPLYKIEDDMYAAAVAAARAAVTQAEATLRSAEKD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRAEELFGNNAGSEQRLDTAVATRDAAAAALQMARAELKTAEINLSRTTVRAPIAGTIG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHEEEECCCEEECC
LSQTTTGALVAEGQEATLTTIRSLDRVHVDVTQSATDLLKWRSRTGKNTVGRVDENVALI
CCCCCCCEEEECCCCHHHHHHHHHHHEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
LADGSRYPLRGRLAAAEPQVQPTTGMVTLRIHFPNPQRILLPGMYVQVELPVARADKAVA
EECCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCEEEECCEEEEEEECHHCCCCEEE
VPHQAVIRDRLGVPAVWLVTEQNVIEARPVEIVKSDGDRWIVTKGLETGDRVVVAGFQKA
CCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCEEEEEECHHH
AVGAPVAPEEQGALPGPAVKAEAATAN
HCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFPRLPLVLAALILAAPAVRAEGPAGAERPPSPVTVVTLTAKDYPIISILPGRVTASEV
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCHHHH
AEVRPQVNGIIRERLFEEGTEVEKDQPLYKIEDDMYAAAVAAARAAVTQAEATLRSAEKD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRAEELFGNNAGSEQRLDTAVATRDAAAAALQMARAELKTAEINLSRTTVRAPIAGTIG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHEEEECCCEEECC
LSQTTTGALVAEGQEATLTTIRSLDRVHVDVTQSATDLLKWRSRTGKNTVGRVDENVALI
CCCCCCCEEEECCCCHHHHHHHHHHHEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
LADGSRYPLRGRLAAAEPQVQPTTGMVTLRIHFPNPQRILLPGMYVQVELPVARADKAVA
EECCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCEEEECCEEEEEEECHHCCCCEEE
VPHQAVIRDRLGVPAVWLVTEQNVIEARPVEIVKSDGDRWIVTKGLETGDRVVVAGFQKA
CCHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCEEEEEECHHH
AVGAPVAPEEQGALPGPAVKAEAATAN
HCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9037074 [H]