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The map label for this gene is braD [H]

Identifier: 77465299

GI number: 77465299

Start: 353580

End: 354467

Strand: Reverse

Name: braD [H]

Synonym: RSP_3294

Alternate gene names: 77465299

Gene position: 354467-353580 (Counterclockwise)

Preceding gene: 77465300

Following gene: 77465298

Centisome position: 37.59

GC content: 65.2

Gene sequence:

>888_bases
ATGATGACGATCTTCGGCATTCCGACGGCGGCCCTCTTCGGCCAGCTGCTGGTGGGTCTCATCAACGGCTCGTTCTATGC
GCTCCTGTCGCTGGGGCTTGCGGTGATCTTCGGCCTTCTCCGGGTCATCAACTTCGCCCATGGCGCGCAATATATGGCCG
GCGCCTTCGCGGCGCTCCTCCTGCTCACCCAGACCGGGATCGGCTACTGGCCCGCGCTGATCCTCGCCCCGCTCATCGTG
GGTCTGGGCGGCGCGCTGATCGAGCGCACCATGCTCTCGCGGCTCTACAAGCTCGACCCGCTCTACGGGCTGCTCTTCAC
CTTCGGCCTTGCGCTCACGCTCGAAGGCACGTTCCGCTGGTTCTTCGGCACCTCGGGCCAGCCCTATTCGCCGCCGGCCG
CACTGACCGGAGCGGTCAATCTCGGCTTCATGTTCCTGCCGATCTATCGCGGCTGGGTGGTTGTGGCCTCGCTCGCCGTC
TGCCTCGGCGTCTGGCTTCTGATCGAAAAGACGAAGCTCGGCGCCTATCTGCGGGCCGCGACGGAAAATCCGGTGCTGGT
CCAGAGCTTCGGGGTCAATGTGCCGCTGCTTCTGACCTTCACCTACGGGCTGGGCGCGGCGCTCGCGGGCTTTGCGGGCG
TGCTGGCCGCGCCGGTCTATCAGGTCTCGCCGCTCATGGGGTCGAACCTCATCATCGTCGTCTTCGCGGTCGTCGTGGTG
GGAGGCATGGGCTCGATCCTCGGCGCGATCGTGACCGGCTATCTGCTCGGCATCGCCGAGGGGCTCACCAAGGTCTTCTA
TCCCGAGGCCTCGAACATCGTGATCTTCGTCATCATGGCGATCGTCCTGATCCTGCGGCCTGCGGGACTCTTCGGAAAGG
ATGCGTGA

Upstream 100 bases:

>100_bases
CGTTTCTACCTCATGGACCACGGACAGATGGTGGCGGATTTCCCGGTCGAGGAGCTGCCCGCGAACATGCCGATGCTGAA
TTCGGTGCTGGGGGTGTGAG

Downstream 100 bases:

>100_bases
CATGGCGCGCGGTTCTGCATCTCCGGCCGCCCCTGCGGTGGCCGCCCATCCCCGGGCCGGCACGCTGAAGATCGTGCTGC
TCCTGATCGCCGCCGCGGCG

Product: ABC branched-chain amino acid transporter, inner membrane subunit

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MMTIFGIPTAALFGQLLVGLINGSFYALLSLGLAVIFGLLRVINFAHGAQYMAGAFAALLLLTQTGIGYWPALILAPLIV
GLGGALIERTMLSRLYKLDPLYGLLFTFGLALTLEGTFRWFFGTSGQPYSPPAALTGAVNLGFMFLPIYRGWVVVASLAV
CLGVWLLIEKTKLGAYLRAATENPVLVQSFGVNVPLLLTFTYGLGAALAGFAGVLAAPVYQVSPLMGSNLIIVVFAVVVV
GGMGSILGAIVTGYLLGIAEGLTKVFYPEASNIVIFVIMAIVLILRPAGLFGKDA

Sequences:

>Translated_295_residues
MMTIFGIPTAALFGQLLVGLINGSFYALLSLGLAVIFGLLRVINFAHGAQYMAGAFAALLLLTQTGIGYWPALILAPLIV
GLGGALIERTMLSRLYKLDPLYGLLFTFGLALTLEGTFRWFFGTSGQPYSPPAALTGAVNLGFMFLPIYRGWVVVASLAV
CLGVWLLIEKTKLGAYLRAATENPVLVQSFGVNVPLLLTFTYGLGAALAGFAGVLAAPVYQVSPLMGSNLIIVVFAVVVV
GGMGSILGAIVTGYLLGIAEGLTKVFYPEASNIVIFVIMAIVLILRPAGLFGKDA
>Mature_295_residues
MMTIFGIPTAALFGQLLVGLINGSFYALLSLGLAVIFGLLRVINFAHGAQYMAGAFAALLLLTQTGIGYWPALILAPLIV
GLGGALIERTMLSRLYKLDPLYGLLFTFGLALTLEGTFRWFFGTSGQPYSPPAALTGAVNLGFMFLPIYRGWVVVASLAV
CLGVWLLIEKTKLGAYLRAATENPVLVQSFGVNVPLLLTFTYGLGAALAGFAGVLAAPVYQVSPLMGSNLIIVVFAVVVV
GGMGSILGAIVTGYLLGIAEGLTKVFYPEASNIVIFVIMAIVLILRPAGLFGKDA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=294, Percent_Identity=27.5510204081633, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31002; Mature: 31002

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTIFGIPTAALFGQLLVGLINGSFYALLSLGLAVIFGLLRVINFAHGAQYMAGAFAALL
CEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTQTGIGYWPALILAPLIVGLGGALIERTMLSRLYKLDPLYGLLFTFGLALTLEGTFRW
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCEEE
FFGTSGQPYSPPAALTGAVNLGFMFLPIYRGWVVVASLAVCLGVWLLIEKTKLGAYLRAA
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TENPVLVQSFGVNVPLLLTFTYGLGAALAGFAGVLAAPVYQVSPLMGSNLIIVVFAVVVV
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GGMGSILGAIVTGYLLGIAEGLTKVFYPEASNIVIFVIMAIVLILRPAGLFGKDA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MMTIFGIPTAALFGQLLVGLINGSFYALLSLGLAVIFGLLRVINFAHGAQYMAGAFAALL
CEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTQTGIGYWPALILAPLIVGLGGALIERTMLSRLYKLDPLYGLLFTFGLALTLEGTFRW
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCEEE
FFGTSGQPYSPPAALTGAVNLGFMFLPIYRGWVVVASLAVCLGVWLLIEKTKLGAYLRAA
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TENPVLVQSFGVNVPLLLTFTYGLGAALAGFAGVLAAPVYQVSPLMGSNLIIVVFAVVVV
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GGMGSILGAIVTGYLLGIAEGLTKVFYPEASNIVIFVIMAIVLILRPAGLFGKDA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]