Click here to switch to the map view.

The map label for this gene is livM [H]

Identifier: 77465298

GI number: 77465298

Start: 352580

End: 353578

Strand: Reverse

Name: livM [H]

Synonym: RSP_3293

Alternate gene names: 77465298

Gene position: 353578-352580 (Counterclockwise)

Preceding gene: 77465299

Following gene: 77465297

Centisome position: 37.49

GC content: 66.57

Gene sequence:

>999_bases
ATGGCGCGCGGTTCTGCATCTCCGGCCGCCCCTGCGGTGGCCGCCCATCCCCGGGCCGGCACGCTGAAGATCGTGCTGCT
CCTGATCGCCGCCGCGGCGCTGATCGCGGCCCCCTTCTTCCTCTATCCGATCTTCCTGATGAAGCTGCTGTGCTTCGCCC
TGTTCGCGGCCGCCTTCAACCTGCTTCTGGGCTACACGGGCCTTCTCTCCTTCGGCCATGCCACCTTCTTCGGCGGCTCG
GCCTATTTCACCGCCCATGCGGTGAAGGTGTGGGGCTGGCCGCCCGAGTTCGGCATCCTTCTGGGCGTGGCGGGCGCGGC
GGCTCTGGGGCTCCTGATGGGGGTCATCGCGATCCGGCGGCAGGGGATCTACTTCGCCATGATCACGCTCGCCCTCAGCC
AGATGTTCTTCTTCTTCTGCCTGCAGGCGGCCTTCACCCATGGCGAGGACGGGATCCAGTCGGTCCCGCGCGGCCATCTC
TTCGGCGTCATCGACCTCTCGAACGCGACCAACATGTATTTCTTCGTGCTGGGCACCTTCACGCTGGGCATGCTCGCGAT
CTGGCGCTTCGTGAATTCGCCCTTCGGCATGATCCTGAAGGCCATTCGCGAGAACGAGCCTCGGGCGATCTCGCTCGGCT
ATTCGGTCGCGGAATACAAGCTCGGCGCCTTCGTGATGTCTGCCGCGCTGGCGGGCCTCGCGGGCGGGGTCAAGGCGCTG
GTCTTCCAGTTCGCCACCCTCACCGATGTGGGCTGGCAGATGTCGGGCGAGGTCATCCTGATGACGCTCCTCGGCGGGAT
CGGCACGCTCGTCGGGCCGGTCGTGGGCTCGGCACTGGTGGTGACGCTGCAGAACTACCTCGCCACCTCGCAATTCCCGG
TCACGATCATCACCGGCCTCGTCTTCATGGCCTGCGTGCTGCTCTTCCGCCGCGGGATCGTGGGCGAGTTCTATGCCTCG
CGCCTCGGCCGCCGGCTCGGCTTCCGGTCGGAGAGCTGA

Upstream 100 bases:

>100_bases
AAGGTCTTCTATCCCGAGGCCTCGAACATCGTGATCTTCGTCATCATGGCGATCGTCCTGATCCTGCGGCCTGCGGGACT
CTTCGGAAAGGATGCGTGAC

Downstream 100 bases:

>100_bases
GATGGAGGCCGATTACATCATCGTGGGGGCGGGCAGCGCGGGCTGCGTACTGGCGAACCGCCTGTCCAAGGATCCCTCGA
ACCGCGTGCTGCTGATCGAG

Product: ABC branched-chain amino acid transporter, inner membrane subunit

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MARGSASPAAPAVAAHPRAGTLKIVLLLIAAAALIAAPFFLYPIFLMKLLCFALFAAAFNLLLGYTGLLSFGHATFFGGS
AYFTAHAVKVWGWPPEFGILLGVAGAAALGLLMGVIAIRRQGIYFAMITLALSQMFFFFCLQAAFTHGEDGIQSVPRGHL
FGVIDLSNATNMYFFVLGTFTLGMLAIWRFVNSPFGMILKAIRENEPRAISLGYSVAEYKLGAFVMSAALAGLAGGVKAL
VFQFATLTDVGWQMSGEVILMTLLGGIGTLVGPVVGSALVVTLQNYLATSQFPVTIITGLVFMACVLLFRRGIVGEFYAS
RLGRRLGFRSES

Sequences:

>Translated_332_residues
MARGSASPAAPAVAAHPRAGTLKIVLLLIAAAALIAAPFFLYPIFLMKLLCFALFAAAFNLLLGYTGLLSFGHATFFGGS
AYFTAHAVKVWGWPPEFGILLGVAGAAALGLLMGVIAIRRQGIYFAMITLALSQMFFFFCLQAAFTHGEDGIQSVPRGHL
FGVIDLSNATNMYFFVLGTFTLGMLAIWRFVNSPFGMILKAIRENEPRAISLGYSVAEYKLGAFVMSAALAGLAGGVKAL
VFQFATLTDVGWQMSGEVILMTLLGGIGTLVGPVVGSALVVTLQNYLATSQFPVTIITGLVFMACVLLFRRGIVGEFYAS
RLGRRLGFRSES
>Mature_331_residues
ARGSASPAAPAVAAHPRAGTLKIVLLLIAAAALIAAPFFLYPIFLMKLLCFALFAAAFNLLLGYTGLLSFGHATFFGGSA
YFTAHAVKVWGWPPEFGILLGVAGAAALGLLMGVIAIRRQGIYFAMITLALSQMFFFFCLQAAFTHGEDGIQSVPRGHLF
GVIDLSNATNMYFFVLGTFTLGMLAIWRFVNSPFGMILKAIRENEPRAISLGYSVAEYKLGAFVMSAALAGLAGGVKALV
FQFATLTDVGWQMSGEVILMTLLGGIGTLVGPVVGSALVVTLQNYLATSQFPVTIITGLVFMACVLLFRRGIVGEFYASR
LGRRLGFRSES

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=287, Percent_Identity=26.4808362369338, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35341; Mature: 35209

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGSASPAAPAVAAHPRAGTLKIVLLLIAAAALIAAPFFLYPIFLMKLLCFALFAAAFN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLGYTGLLSFGHATFFGGSAYFTAHAVKVWGWPPEFGILLGVAGAAALGLLMGVIAIRR
HHHHHHHHHHHCCHHHCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QGIYFAMITLALSQMFFFFCLQAAFTHGEDGIQSVPRGHLFGVIDLSNATNMYFFVLGTF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
TLGMLAIWRFVNSPFGMILKAIRENEPRAISLGYSVAEYKLGAFVMSAALAGLAGGVKAL
HHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
VFQFATLTDVGWQMSGEVILMTLLGGIGTLVGPVVGSALVVTLQNYLATSQFPVTIITGL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VFMACVLLFRRGIVGEFYASRLGRRLGFRSES
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARGSASPAAPAVAAHPRAGTLKIVLLLIAAAALIAAPFFLYPIFLMKLLCFALFAAAFN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLGYTGLLSFGHATFFGGSAYFTAHAVKVWGWPPEFGILLGVAGAAALGLLMGVIAIRR
HHHHHHHHHHHCCHHHCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QGIYFAMITLALSQMFFFFCLQAAFTHGEDGIQSVPRGHLFGVIDLSNATNMYFFVLGTF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
TLGMLAIWRFVNSPFGMILKAIRENEPRAISLGYSVAEYKLGAFVMSAALAGLAGGVKAL
HHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
VFQFATLTDVGWQMSGEVILMTLLGGIGTLVGPVVGSALVVTLQNYLATSQFPVTIITGL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VFMACVLLFRRGIVGEFYASRLGRRLGFRSES
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]