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The map label for this gene is gsiB [H]

Identifier: 77465255

GI number: 77465255

Start: 304951

End: 306462

Strand: Reverse

Name: gsiB [H]

Synonym: RSP_3248

Alternate gene names: 77465255

Gene position: 306462-304951 (Counterclockwise)

Preceding gene: 77465256

Following gene: 77465251

Centisome position: 32.5

GC content: 62.96

Gene sequence:

>1512_bases
ATGACAGGAAAATGGGCCCGCGTCGGCCTCATCGCGCTGAGCGTGACCCTCGGCCTCGGCAGCATCGCCGAAGCCGCGGG
CGTGCTGACCATCGGCCGGCGCGAGGACGGCACCACCTTCGACCCGATCGCCACGGCGCAGAACGTGGATTTCTGGGTCT
TCTCGAACGTCTATGACGTGCTCGTGCGCGTGGACAAGACCGGGACCAAGCTCGAGCCCGGTCTGGCCGAAAGCTGGGAG
ATCTCGGAGGACGGGCTCACCTACACGTTCCATCTGCGCGACGCGAACTTCTCCGACGGCTCGCCCATCACCGCCGAGGA
CGCGGCCTTCACGCTGCTGCGCATCCGCGACAGCGAACTGTCGCTCTGGTCGGACAGCTATGCGGTGATCGAAACTGCCG
AGGCGACCGATCCGAAGACCCTCGTCGTCAAGCTGAAGACCCCCTCGGCGCCCTTCCTGTCCACCATGGCGATGCCCGCC
GTCTCGATCCTGTCGAAGGCGGGCGTCGAAGCCATGGGCGAGGAGGCCTATGCCGAGAAGCCCGTGGCCTCGGGCGCCTT
TACCGTCAAGGAATGGCGCCGCGGCGACCGGGTGATCCTGAAGAAGAACCCGGAATTCTGGCAAGCCGACCGGGTGAGCC
TCGACGGGGTCGAATGGATCTCGATCCCCGACGACAACAGCCGGATGCTGAGCGTGCAGGCGGGCGAACTCGACGCCGCG
ATCTTCGTGCCCTTCTCGCGGGTGGCGGAACTGAAGAAGGATACCAACCTCAAGGTCATGGTCGAGCCCTCGACGCGCGA
GGACCATCTGCTCCTCAACCACGAGCACGAGCCACTGAACGATCCCAAGGTGCGCGAGGCGATCGATCTCGCCATCGACA
AGCAGGCGATCGTCGACACGGTGACCTTCGGGCAGGCGCAGATCGCCAATTCCTACATCCCCGCCGGCGCGCTCTATCAC
AACGACAACAACCTGCTGCGGCCCCACGACCCCGAGAAGGCCAAGGCGCTTCTGGCCGAGGCGGGTGTGTCCGACGTGAG
CCTCGATTATGTGGTGAATGCGGGCAACGAGGTGGACGAGCAGATCGCCGTCCTCCTCCAGCAGCAGCTGGGTCAGGCCG
GGATCACGGTCAATCTGCAGAAGATGGACCCAAGCATGACCTGGGACATGCTGGTGAATGGCGAATACGACCTGTCGGTC
ATGTATTGGACGAACGACATCCTCGATCCCGACCAGAAGACCACCTTCGTTCTGGGTCACGACGTCAACATGAACTACAT
GACGCGCTACGAAAACGACACGGTGAAGCAGCTCGTGGCCGATGCCCGGCTCGAGATGGACCCGGCCAAGCGCGAGGCGA
TGTATACCGAGATCCAGGAACTGTCGAAGGCCGATACCCACTGGATCGACCTCTATTACAGCCCCTTCATCAACGTGACG
CGTGCCAATATCGAGAACTTCAACCAGAACCCGCTGGGCCGCTTCTTCCTCGAGGATACGGTGAAGAACTGA

Upstream 100 bases:

>100_bases
CGGCGCTGATCGCGGCCATCCCGAGCATCGACCCCGACCAGCCGCTGGCGGCCGCGATGCCCGACCCCAGAAGCCAGACC
CAACAGGAAGGACTGCTCAC

Downstream 100 bases:

>100_bases
GGCTCCGCCTCTGGCGGACCAAGGGGGTCGGCGCGGTGCGCCGGCCCCTTTTTTGGCTCAGACCCGGGCGTAGTCGATGC
GCCGCGCGAGCCAGCAGCCG

Product: ABC peptide transporter, periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTGKWARVGLIALSVTLGLGSIAEAAGVLTIGRREDGTTFDPIATAQNVDFWVFSNVYDVLVRVDKTGTKLEPGLAESWE
ISEDGLTYTFHLRDANFSDGSPITAEDAAFTLLRIRDSELSLWSDSYAVIETAEATDPKTLVVKLKTPSAPFLSTMAMPA
VSILSKAGVEAMGEEAYAEKPVASGAFTVKEWRRGDRVILKKNPEFWQADRVSLDGVEWISIPDDNSRMLSVQAGELDAA
IFVPFSRVAELKKDTNLKVMVEPSTREDHLLLNHEHEPLNDPKVREAIDLAIDKQAIVDTVTFGQAQIANSYIPAGALYH
NDNNLLRPHDPEKAKALLAEAGVSDVSLDYVVNAGNEVDEQIAVLLQQQLGQAGITVNLQKMDPSMTWDMLVNGEYDLSV
MYWTNDILDPDQKTTFVLGHDVNMNYMTRYENDTVKQLVADARLEMDPAKREAMYTEIQELSKADTHWIDLYYSPFINVT
RANIENFNQNPLGRFFLEDTVKN

Sequences:

>Translated_503_residues
MTGKWARVGLIALSVTLGLGSIAEAAGVLTIGRREDGTTFDPIATAQNVDFWVFSNVYDVLVRVDKTGTKLEPGLAESWE
ISEDGLTYTFHLRDANFSDGSPITAEDAAFTLLRIRDSELSLWSDSYAVIETAEATDPKTLVVKLKTPSAPFLSTMAMPA
VSILSKAGVEAMGEEAYAEKPVASGAFTVKEWRRGDRVILKKNPEFWQADRVSLDGVEWISIPDDNSRMLSVQAGELDAA
IFVPFSRVAELKKDTNLKVMVEPSTREDHLLLNHEHEPLNDPKVREAIDLAIDKQAIVDTVTFGQAQIANSYIPAGALYH
NDNNLLRPHDPEKAKALLAEAGVSDVSLDYVVNAGNEVDEQIAVLLQQQLGQAGITVNLQKMDPSMTWDMLVNGEYDLSV
MYWTNDILDPDQKTTFVLGHDVNMNYMTRYENDTVKQLVADARLEMDPAKREAMYTEIQELSKADTHWIDLYYSPFINVT
RANIENFNQNPLGRFFLEDTVKN
>Mature_502_residues
TGKWARVGLIALSVTLGLGSIAEAAGVLTIGRREDGTTFDPIATAQNVDFWVFSNVYDVLVRVDKTGTKLEPGLAESWEI
SEDGLTYTFHLRDANFSDGSPITAEDAAFTLLRIRDSELSLWSDSYAVIETAEATDPKTLVVKLKTPSAPFLSTMAMPAV
SILSKAGVEAMGEEAYAEKPVASGAFTVKEWRRGDRVILKKNPEFWQADRVSLDGVEWISIPDDNSRMLSVQAGELDAAI
FVPFSRVAELKKDTNLKVMVEPSTREDHLLLNHEHEPLNDPKVREAIDLAIDKQAIVDTVTFGQAQIANSYIPAGALYHN
DNNLLRPHDPEKAKALLAEAGVSDVSLDYVVNAGNEVDEQIAVLLQQQLGQAGITVNLQKMDPSMTWDMLVNGEYDLSVM
YWTNDILDPDQKTTFVLGHDVNMNYMTRYENDTVKQLVADARLEMDPAKREAMYTEIQELSKADTHWIDLYYSPFINVTR
ANIENFNQNPLGRFFLEDTVKN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=506, Percent_Identity=29.4466403162055, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787762, Length=496, Percent_Identity=28.4274193548387, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789966, Length=526, Percent_Identity=26.615969581749, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1787495, Length=434, Percent_Identity=26.4976958525346, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1787551, Length=473, Percent_Identity=24.7357293868922, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1789397, Length=491, Percent_Identity=25.8655804480652, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1789887, Length=343, Percent_Identity=26.2390670553936, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI87081878, Length=488, Percent_Identity=23.155737704918, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87082063, Length=374, Percent_Identity=21.9251336898396, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55958; Mature: 55826

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKWARVGLIALSVTLGLGSIAEAAGVLTIGRREDGTTFDPIATAQNVDFWVFSNVYDV
CCCCCHHHEEEEEEEHHCCCHHHHHCCEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHEE
LVRVDKTGTKLEPGLAESWEISEDGLTYTFHLRDANFSDGSPITAEDAAFTLLRIRDSEL
EEEECCCCCCCCCCCCCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCC
SLWSDSYAVIETAEATDPKTLVVKLKTPSAPFLSTMAMPAVSILSKAGVEAMGEEAYAEK
EEECCCEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHCC
PVASGAFTVKEWRRGDRVILKKNPEFWQADRVSLDGVEWISIPDDNSRMLSVQAGELDAA
CCCCCCEEHHHHCCCCEEEEECCCCCCCCCCEEECCEEEEECCCCCCEEEEEECCCCCEE
IFVPFSRVAELKKDTNLKVMVEPSTREDHLLLNHEHEPLNDPKVREAIDLAIDKQAIVDT
EECCHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHEE
VTFGQAQIANSYIPAGALYHNDNNLLRPHDPEKAKALLAEAGVSDVSLDYVVNAGNEVDE
EECCCHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCHHH
QIAVLLQQQLGQAGITVNLQKMDPSMTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGH
HHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCEEEEEEEEECCCCCCCCCEEEEEEE
DVNMNYMTRYENDTVKQLVADARLEMDPAKREAMYTEIQELSKADTHWIDLYYSPFINVT
CCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEE
RANIENFNQNPLGRFFLEDTVKN
ECCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TGKWARVGLIALSVTLGLGSIAEAAGVLTIGRREDGTTFDPIATAQNVDFWVFSNVYDV
CCCCHHHEEEEEEEHHCCCHHHHHCCEEEEECCCCCCCCCCCCCCCCCCEEEHHHHHEE
LVRVDKTGTKLEPGLAESWEISEDGLTYTFHLRDANFSDGSPITAEDAAFTLLRIRDSEL
EEEECCCCCCCCCCCCCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCC
SLWSDSYAVIETAEATDPKTLVVKLKTPSAPFLSTMAMPAVSILSKAGVEAMGEEAYAEK
EEECCCEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHCC
PVASGAFTVKEWRRGDRVILKKNPEFWQADRVSLDGVEWISIPDDNSRMLSVQAGELDAA
CCCCCCEEHHHHCCCCEEEEECCCCCCCCCCEEECCEEEEECCCCCCEEEEEECCCCCEE
IFVPFSRVAELKKDTNLKVMVEPSTREDHLLLNHEHEPLNDPKVREAIDLAIDKQAIVDT
EECCHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHEE
VTFGQAQIANSYIPAGALYHNDNNLLRPHDPEKAKALLAEAGVSDVSLDYVVNAGNEVDE
EECCCHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCHHH
QIAVLLQQQLGQAGITVNLQKMDPSMTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGH
HHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCEEEEEEEEECCCCCCCCCEEEEEEE
DVNMNYMTRYENDTVKQLVADARLEMDPAKREAMYTEIQELSKADTHWIDLYYSPFINVT
CCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEE
RANIENFNQNPLGRFFLEDTVKN
ECCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA