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The map label for this gene is nodW [H]

Identifier: 77461928

GI number: 77461928

Start: 6397369

End: 6398034

Strand: Reverse

Name: nodW [H]

Synonym: Pfl01_5708

Alternate gene names: 77461928

Gene position: 6398034-6397369 (Counterclockwise)

Preceding gene: 77461930

Following gene: 77461924

Centisome position: 99.37

GC content: 57.81

Gene sequence:

>666_bases
ATGCATTCAACACACCTGATCGTCCCTGCACGCACAGCCGCGCCCATGACCGAACCCCTGCCGATCGTATTCGTTGTCGA
CGATGATGTTTCCGTACGCGAATCCCTAGAGCTGCTGATACGGTTTTCGCAGTGGCAGCCTCAACTGTTCGAATCCGCCC
AGGCCTTTCTCGGACAGCCGCGCAGCCCCGTGCCCAGCTGCCTGGTGCTGGACATCAACCTGCCCGACCTAAATGGCCTG
GATTTGCAGGCTACCATCGCCCGAAGATATCCCGACACGCCGATCATCTTCATCACCGGCTATGGTGACATCCCGCTGAC
GGTGCGCGCGATGAAAGCTGGGGCCGTGGAGTTCTTGACCAAGCCCTTCAATGACGACGTGCTTCTCGCCGCCATTGCCA
ATGCACTGGAAAAAAGCCGTTCCTTGCTGGGCCAGGAAGATCAACTGCGTACTCTGCGAACCGCCTACGCAACCCTGACG
CCAAGGGAACAGGAGGTGATGACCAAGGTGGTTGCGGGACAAATGAACAAGATGATTGCCTGCGACCTGGCCATCAGCGA
GATCACTGTCAAAGCCCATCGCGGAAAAGTGATGCGCAAAATGAAGGCCCGCTCTTTGGCGGGCCTAGTCAAGATGGCGG
CGTTACTAGCAATGCCGCTGCATTGA

Upstream 100 bases:

>100_bases
GGAATGCCTGATGAAACCGTTCAGCGAAGCCCAGTTGCTCACAGCGCTGAGCGCGGCGCTTCCGGTGGACTGAGCGGGCT
AATTTGAAGGAACTATTTTG

Downstream 100 bases:

>100_bases
GTCACTGCGAAGCTTTCGCCTCGTTCAACAGAGTCTGGATCATCTGCTCCTGCACCTTATAACTACCTTCGCCAAAGTGG
GTGTAACGTATCTGGCCCTT

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MHSTHLIVPARTAAPMTEPLPIVFVVDDDVSVRESLELLIRFSQWQPQLFESAQAFLGQPRSPVPSCLVLDINLPDLNGL
DLQATIARRYPDTPIIFITGYGDIPLTVRAMKAGAVEFLTKPFNDDVLLAAIANALEKSRSLLGQEDQLRTLRTAYATLT
PREQEVMTKVVAGQMNKMIACDLAISEITVKAHRGKVMRKMKARSLAGLVKMAALLAMPLH

Sequences:

>Translated_221_residues
MHSTHLIVPARTAAPMTEPLPIVFVVDDDVSVRESLELLIRFSQWQPQLFESAQAFLGQPRSPVPSCLVLDINLPDLNGL
DLQATIARRYPDTPIIFITGYGDIPLTVRAMKAGAVEFLTKPFNDDVLLAAIANALEKSRSLLGQEDQLRTLRTAYATLT
PREQEVMTKVVAGQMNKMIACDLAISEITVKAHRGKVMRKMKARSLAGLVKMAALLAMPLH
>Mature_221_residues
MHSTHLIVPARTAAPMTEPLPIVFVVDDDVSVRESLELLIRFSQWQPQLFESAQAFLGQPRSPVPSCLVLDINLPDLNGL
DLQATIARRYPDTPIIFITGYGDIPLTVRAMKAGAVEFLTKPFNDDVLLAAIANALEKSRSLLGQEDQLRTLRTAYATLT
PREQEVMTKVVAGQMNKMIACDLAISEITVKAHRGKVMRKMKARSLAGLVKMAALLAMPLH

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=35.6521739130435, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790437, Length=179, Percent_Identity=25.6983240223464, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790102, Length=182, Percent_Identity=28.021978021978, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24342; Mature: 24342

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSTHLIVPARTAAPMTEPLPIVFVVDDDVSVRESLELLIRFSQWQPQLFESAQAFLGQP
CCCCEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
RSPVPSCLVLDINLPDLNGLDLQATIARRYPDTPIIFITGYGDIPLTVRAMKAGAVEFLT
CCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEHHCCHHHHHH
KPFNDDVLLAAIANALEKSRSLLGQEDQLRTLRTAYATLTPREQEVMTKVVAGQMNKMIA
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEE
CDLAISEITVKAHRGKVMRKMKARSLAGLVKMAALLAMPLH
EEEEHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHSTHLIVPARTAAPMTEPLPIVFVVDDDVSVRESLELLIRFSQWQPQLFESAQAFLGQP
CCCCEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
RSPVPSCLVLDINLPDLNGLDLQATIARRYPDTPIIFITGYGDIPLTVRAMKAGAVEFLT
CCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEHHCCHHHHHH
KPFNDDVLLAAIANALEKSRSLLGQEDQLRTLRTAYATLTPREQEVMTKVVAGQMNKMIA
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEE
CDLAISEITVKAHRGKVMRKMKARSLAGLVKMAALLAMPLH
EEEEHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]