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The map label for this gene is nodV [H]

Identifier: 77461930

GI number: 77461930

Start: 6398433

End: 6399980

Strand: Reverse

Name: nodV [H]

Synonym: Pfl01_5710

Alternate gene names: 77461930

Gene position: 6399980-6398433 (Counterclockwise)

Preceding gene: 77461931

Following gene: 77461928

Centisome position: 99.4

GC content: 61.89

Gene sequence:

>1548_bases
ATGTCGAATGAATTCGATCGCGTCGTCGATGCGTTGCCTGGCCTGGCGTTCACCACCCGGGAGGACGGCAGCGTCGACTG
CGTCAACCACGCCTGGTGCGCCTACACAGGGCACAGCGCACACCAGGTTGCCGATGCCGGCTGGCTGTCGGCTGTGCATC
CGGAAGACCTGTCCGGTACGATCAACGCCTGGAAAAACGCGGTGCATAGTGGTCAGCCCTATCGCAAAGAACTGCGCCTG
CAACGAGCGGACGGCACTTACCGCTGGTTCGTCAGCGACGGCAAACCGATGCGTGATGACCAGGGCATGATTCTGCGCTG
GTGGGTGATCAGCGTGGACATCGACGAGCAGAAGCGCGACAAGGCACTGATCGCACGCACGCTGGCCGAAGTAAGCGCCT
CGCAGGATCGTCTGAACACCATCATCAACGCCATCCCCGGGTTCGTGTGGAGCGCCACGCCCGATGGCAGCGTCAGTTTC
GTCAACCAGCGCTGGTGCGATTTCACCGGCATGAGCCTGGACGAGGCGCGCGGAGACGGCTGGGTGCGCGCGGTGCACCC
CGACGACGCCGGCCCCCTGGCCCAGCGCTGGGGTGCGTTGCTGCAAACCGGTGAAGCCGGAGAATACGAAACCCGGTTGC
GCCGCTTCGACGGCGACTATCGCTGGTTTATCGTAAGGGCGGTGCCGCAGCGTGATGCTTGGGGCGCGGTTACGTGCTGG
TACGGTGAGAACACCGATATCGAAGACCGCAAGCGTGCCGAAATGCTCGCCGCCAGCAGGGCCATCGAGCATCGATTGTC
GGAAGAAACCCTGAGCAAGGTGCGCTCGGAACTGGCGCACTTGGCGCGAGTGGCTAGCCTTAGCGCGGTGACCGCCTCGA
TTGCACATGAAGTCAATCAGCCCTTGGCCGGCATTATCACCAATGCCAGCACTTGCCTGCGCATGCTGGGCGCCGACCCG
CCGAATGTCACAGGCGCCCTGGAAACCGCGCGGCGCACCATTCGCGATGGTAACCGTGCCGCCGATGTGATCTACCGGCT
GCGCGCGTTGTTTTCGAAAAAAGATATCACCCTTGAAAGCATCGATCTGAACGAAGCCGTGCGGGAAGTCATCGCCATGC
TGCTCAGTGAACTGCAGCGCAATGGCGTGACCTTGCACCCGGAACTCGCCGACCACCTGCCGCAAGTCAAGGCCGACCGC
GTGCACGTGCAACAAGTCATCCTCAACCTAATTCTGAATGCCATCGAGGCGATGAAAACCATGACTGGCCGCACGCGACA
CCTGCACGTCACCACCGGCCTGGACGAACACCGGCAAGTCTGCTTTGCCGTAGAGGACAATGGCTGCGGTTTCGACCCGC
TGGACGCCGAGCGCCTGTTCACGGCGTTCTACACCACCAAGACCACCGGCATGGGCATTGGATTGTCCATTAGCCGCTCG
ATCATCGAGCAGCATGACGGGGTTCTGTGGGCCTGTCTCAATAACGAGGCTGGCGCCACGTTTCAATTCACCCTGCCCCC
GGTGACCGATACAAAGGAGGGTATCTGA

Upstream 100 bases:

>100_bases
TGATGGCCGCCGCGTGTGACATGTGACACCTAAGTGTGATGGGCGTACTCCGGCCCACAGGGCATCCTGACCCCACGACG
CCTGTAGATAGAGAAGCGGA

Downstream 100 bases:

>100_bases
TGGAAAAGCCGTTGCTTGTAGCAGTCATCGATGATGATGAGTCGGTACGCGAGTCCCTGCCGGACCTACTCAATGAGTTC
GGGTTTGCGGTGCAGGCGTT

Product: PAS/PAC sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSNEFDRVVDALPGLAFTTREDGSVDCVNHAWCAYTGHSAHQVADAGWLSAVHPEDLSGTINAWKNAVHSGQPYRKELRL
QRADGTYRWFVSDGKPMRDDQGMILRWWVISVDIDEQKRDKALIARTLAEVSASQDRLNTIINAIPGFVWSATPDGSVSF
VNQRWCDFTGMSLDEARGDGWVRAVHPDDAGPLAQRWGALLQTGEAGEYETRLRRFDGDYRWFIVRAVPQRDAWGAVTCW
YGENTDIEDRKRAEMLAASRAIEHRLSEETLSKVRSELAHLARVASLSAVTASIAHEVNQPLAGIITNASTCLRMLGADP
PNVTGALETARRTIRDGNRAADVIYRLRALFSKKDITLESIDLNEAVREVIAMLLSELQRNGVTLHPELADHLPQVKADR
VHVQQVILNLILNAIEAMKTMTGRTRHLHVTTGLDEHRQVCFAVEDNGCGFDPLDAERLFTAFYTTKTTGMGIGLSISRS
IIEQHDGVLWACLNNEAGATFQFTLPPVTDTKEGI

Sequences:

>Translated_515_residues
MSNEFDRVVDALPGLAFTTREDGSVDCVNHAWCAYTGHSAHQVADAGWLSAVHPEDLSGTINAWKNAVHSGQPYRKELRL
QRADGTYRWFVSDGKPMRDDQGMILRWWVISVDIDEQKRDKALIARTLAEVSASQDRLNTIINAIPGFVWSATPDGSVSF
VNQRWCDFTGMSLDEARGDGWVRAVHPDDAGPLAQRWGALLQTGEAGEYETRLRRFDGDYRWFIVRAVPQRDAWGAVTCW
YGENTDIEDRKRAEMLAASRAIEHRLSEETLSKVRSELAHLARVASLSAVTASIAHEVNQPLAGIITNASTCLRMLGADP
PNVTGALETARRTIRDGNRAADVIYRLRALFSKKDITLESIDLNEAVREVIAMLLSELQRNGVTLHPELADHLPQVKADR
VHVQQVILNLILNAIEAMKTMTGRTRHLHVTTGLDEHRQVCFAVEDNGCGFDPLDAERLFTAFYTTKTTGMGIGLSISRS
IIEQHDGVLWACLNNEAGATFQFTLPPVTDTKEGI
>Mature_514_residues
SNEFDRVVDALPGLAFTTREDGSVDCVNHAWCAYTGHSAHQVADAGWLSAVHPEDLSGTINAWKNAVHSGQPYRKELRLQ
RADGTYRWFVSDGKPMRDDQGMILRWWVISVDIDEQKRDKALIARTLAEVSASQDRLNTIINAIPGFVWSATPDGSVSFV
NQRWCDFTGMSLDEARGDGWVRAVHPDDAGPLAQRWGALLQTGEAGEYETRLRRFDGDYRWFIVRAVPQRDAWGAVTCWY
GENTDIEDRKRAEMLAASRAIEHRLSEETLSKVRSELAHLARVASLSAVTASIAHEVNQPLAGIITNASTCLRMLGADPP
NVTGALETARRTIRDGNRAADVIYRLRALFSKKDITLESIDLNEAVREVIAMLLSELQRNGVTLHPELADHLPQVKADRV
HVQQVILNLILNAIEAMKTMTGRTRHLHVTTGLDEHRQVCFAVEDNGCGFDPLDAERLFTAFYTTKTTGMGIGLSISRSI
IEQHDGVLWACLNNEAGATFQFTLPPVTDTKEGI

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process. NodV may function as a membrane-associated protein kinase that phosphorylates nodW in response to

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=229, Percent_Identity=30.1310043668122, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790436, Length=235, Percent_Identity=28.5106382978723, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI48994928, Length=379, Percent_Identity=23.7467018469657, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790300, Length=231, Percent_Identity=30.3030303030303, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081816, Length=345, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57238; Mature: 57107

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEFDRVVDALPGLAFTTREDGSVDCVNHAWCAYTGHSAHQVADAGWLSAVHPEDLSGT
CCCHHHHHHHHCCCCEEEECCCCCEEECCCCEEEECCCCHHHHHCCCCCCCCCCCCCCCH
INAWKNAVHSGQPYRKELRLQRADGTYRWFVSDGKPMRDDQGMILRWWVISVDIDEQKRD
HHHHHHHHCCCCCHHHHHHEEECCCEEEEEECCCCCCCCCCCCEEEEEEEEEECCHHHHH
KALIARTLAEVSASQDRLNTIINAIPGFVWSATPDGSVSFVNQRWCDFTGMSLDEARGDG
HHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCEEEHHHHHCCCCCCCHHHCCCCC
WVRAVHPDDAGPLAQRWGALLQTGEAGEYETRLRRFDGDYRWFIVRAVPQRDAWGAVTCW
EEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEE
YGENTDIEDRKRAEMLAASRAIEHRLSEETLSKVRSELAHLARVASLSAVTASIAHEVNQ
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLAGIITNASTCLRMLGADPPNVTGALETARRTIRDGNRAADVIYRLRALFSKKDITLES
CHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
IDLNEAVREVIAMLLSELQRNGVTLHPELADHLPQVKADRVHVQQVILNLILNAIEAMKT
CCHHHHHHHHHHHHHHHHHHCCCEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MTGRTRHLHVTTGLDEHRQVCFAVEDNGCGFDPLDAERLFTAFYTTKTTGMGIGLSISRS
HCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
IIEQHDGVLWACLNNEAGATFQFTLPPVTDTKEGI
HHHHCCCEEEEEECCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SNEFDRVVDALPGLAFTTREDGSVDCVNHAWCAYTGHSAHQVADAGWLSAVHPEDLSGT
CCHHHHHHHHCCCCEEEECCCCCEEECCCCEEEECCCCHHHHHCCCCCCCCCCCCCCCH
INAWKNAVHSGQPYRKELRLQRADGTYRWFVSDGKPMRDDQGMILRWWVISVDIDEQKRD
HHHHHHHHCCCCCHHHHHHEEECCCEEEEEECCCCCCCCCCCCEEEEEEEEEECCHHHHH
KALIARTLAEVSASQDRLNTIINAIPGFVWSATPDGSVSFVNQRWCDFTGMSLDEARGDG
HHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCEEEHHHHHCCCCCCCHHHCCCCC
WVRAVHPDDAGPLAQRWGALLQTGEAGEYETRLRRFDGDYRWFIVRAVPQRDAWGAVTCW
EEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEE
YGENTDIEDRKRAEMLAASRAIEHRLSEETLSKVRSELAHLARVASLSAVTASIAHEVNQ
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLAGIITNASTCLRMLGADPPNVTGALETARRTIRDGNRAADVIYRLRALFSKKDITLES
CHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEE
IDLNEAVREVIAMLLSELQRNGVTLHPELADHLPQVKADRVHVQQVILNLILNAIEAMKT
CCHHHHHHHHHHHHHHHHHHCCCEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MTGRTRHLHVTTGLDEHRQVCFAVEDNGCGFDPLDAERLFTAFYTTKTTGMGIGLSISRS
HCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
IIEQHDGVLWACLNNEAGATFQFTLPPVTDTKEGI
HHHHCCCEEEEEECCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]