Click here to switch to the map view.

The map label for this gene is amiC [H]

Identifier: 77461877

GI number: 77461877

Start: 6337841

End: 6339013

Strand: Reverse

Name: amiC [H]

Synonym: Pfl01_5657

Alternate gene names: 77461877

Gene position: 6339013-6337841 (Counterclockwise)

Preceding gene: 77461884

Following gene: 77461875

Centisome position: 98.46

GC content: 57.2

Gene sequence:

>1173_bases
ATGCTGGCCAGCGCGGCCTTTGTTTTGCCTTTTTCGGTGTCCGCTACGCAGATACGCAATGCGCGGCTTTGGCGTTCGGA
TGACAAGCTTCGGCTGGTGTTCGATCTGAGTGGTTCCGTGAGCTACAAGACGTTTACCTTGAGTGCACCAGAGCGGTTGA
TCATCGATTTGAACGGCGCCAGCCTGATTGGGGACTTCAGCCAATTGACGTTATCCGGCACCCCTATTCATTCGATCCGC
TCCGGCCATTTTGGCCAAGGGAACACACGGATCGTGCTGGATTTAAGCAGTCCGGTTCAGCTCAATTCATTCTTGTTGGC
GCCTCAGGATGGGCAAGGACATCGCCTGGTTTTGGACTTGATGAGCGCATCAGCAGGAGTCCCGGCGAGCGTTCCACGTG
AAACACCCCAGGAAAAATCTCACCCCAAGCGCGACATCATGGTGGTGGTCGACCCAGGTCATGGCGGGAAAGATCCGGGT
GCAATCGGCGCCAAAGGCGAGCGAGAAAAGGATGTGGTGCTGTCCATCGCGCAACTGCTCGCACGTCGGCTTAAACGTGA
GAAAGGCTTTGATGTGAAGCTGGTTCGTAACGACGATTTCTTCGTCCCGTTACGCAAGCGTGTGGAAATCGCGCGTCAGC
ACAAGGCTGACATGTTCATCTCGGTGCACGCCGATGCTGCGCCGCGTCTGACCGCTTCCGGGGCGTCGGTGTATTGCCTG
TCGGAAGGCGGCGCGACGTCCGCCACCGCGCGGTTCATGGCGCAGCGTGAGAACGGTGTGGATCTGCTCGGTGCAACCAG
TCTGCTCAATCTCAAAGACAAGGATCCGATGCTCGCTGGCGTGATCCTCGACATGTCGATGAACGCAACCATCGCCGCCA
GCTTGCAACTCGGAAACACAGTGCTGAGCAGTCTGGCTGGCATTACCACGCTGCACCAAAAGCGCGTGGAACAGGCTGGA
TTTGCCGTTCTGAAATCGCCGGATGTGCCGTCAATCCTGGTGGAAACCGGGTTTATTTCCAACGCACGGGACAGTCAGCG
CTTGGTGACCGCGCGTCATCAGCAGGCCGTGGCGGACGGTTTGTTTGAGGGCTTGCAGCGTTACTTCCAGAAAAACCCGC
CTGTCGACAGCTATATGGCCTGGCAACAAGAGCAAGAAGGATATCGGGTTTAG

Upstream 100 bases:

>100_bases
TCATGGTAATTGAATTTGATGTTACATTATAACAATTCAAACCACGATGCCTTTTTCATGAACAGGCTTTCATATGCACA
GACGTCACTTGCTCAACCTG

Downstream 100 bases:

>100_bases
CAGCCGGTGATCCGGCTGCAGGTGAACTTTGCACTGCTGCCGCCTGAGCTGGAGAACCGATTGATCGTCGTCCAGCCCAC
ACGGCGGTTGTAGCCAACCC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLASAAFVLPFSVSATQIRNARLWRSDDKLRLVFDLSGSVSYKTFTLSAPERLIIDLNGASLIGDFSQLTLSGTPIHSIR
SGHFGQGNTRIVLDLSSPVQLNSFLLAPQDGQGHRLVLDLMSASAGVPASVPRETPQEKSHPKRDIMVVVDPGHGGKDPG
AIGAKGEREKDVVLSIAQLLARRLKREKGFDVKLVRNDDFFVPLRKRVEIARQHKADMFISVHADAAPRLTASGASVYCL
SEGGATSATARFMAQRENGVDLLGATSLLNLKDKDPMLAGVILDMSMNATIAASLQLGNTVLSSLAGITTLHQKRVEQAG
FAVLKSPDVPSILVETGFISNARDSQRLVTARHQQAVADGLFEGLQRYFQKNPPVDSYMAWQQEQEGYRV

Sequences:

>Translated_390_residues
MLASAAFVLPFSVSATQIRNARLWRSDDKLRLVFDLSGSVSYKTFTLSAPERLIIDLNGASLIGDFSQLTLSGTPIHSIR
SGHFGQGNTRIVLDLSSPVQLNSFLLAPQDGQGHRLVLDLMSASAGVPASVPRETPQEKSHPKRDIMVVVDPGHGGKDPG
AIGAKGEREKDVVLSIAQLLARRLKREKGFDVKLVRNDDFFVPLRKRVEIARQHKADMFISVHADAAPRLTASGASVYCL
SEGGATSATARFMAQRENGVDLLGATSLLNLKDKDPMLAGVILDMSMNATIAASLQLGNTVLSSLAGITTLHQKRVEQAG
FAVLKSPDVPSILVETGFISNARDSQRLVTARHQQAVADGLFEGLQRYFQKNPPVDSYMAWQQEQEGYRV
>Mature_390_residues
MLASAAFVLPFSVSATQIRNARLWRSDDKLRLVFDLSGSVSYKTFTLSAPERLIIDLNGASLIGDFSQLTLSGTPIHSIR
SGHFGQGNTRIVLDLSSPVQLNSFLLAPQDGQGHRLVLDLMSASAGVPASVPRETPQEKSHPKRDIMVVVDPGHGGKDPG
AIGAKGEREKDVVLSIAQLLARRLKREKGFDVKLVRNDDFFVPLRKRVEIARQHKADMFISVHADAAPRLTASGASVYCL
SEGGATSATARFMAQRENGVDLLGATSLLNLKDKDPMLAGVILDMSMNATIAASLQLGNTVLSSLAGITTLHQKRVEQAG
FAVLKSPDVPSILVETGFISNARDSQRLVTARHQQAVADGLFEGLQRYFQKNPPVDSYMAWQQEQEGYRV

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=380, Percent_Identity=38.9473684210526, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1788776, Length=233, Percent_Identity=45.9227467811159, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1790611, Length=245, Percent_Identity=37.9591836734694, Blast_Score=182, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 42281; Mature: 42281

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLASAAFVLPFSVSATQIRNARLWRSDDKLRLVFDLSGSVSYKTFTLSAPERLIIDLNGA
CCCCCEEEEEECCCHHHHHCCCEECCCCCEEEEEEECCCEEEEEEEECCCCEEEEEECCC
SLIGDFSQLTLSGTPIHSIRSGHFGQGNTRIVLDLSSPVQLNSFLLAPQDGQGHRLVLDL
HHHCCCHHEEECCCCCHHHCCCCCCCCCCEEEEECCCCCEECEEEEECCCCCCCEEEEEE
MSASAGVPASVPRETPQEKSHPKRDIMVVVDPGHGGKDPGAIGAKGEREKDVVLSIAQLL
HHCCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ARRLKREKGFDVKLVRNDDFFVPLRKRVEIARQHKADMFISVHADAAPRLTASGASVYCL
HHHHHHHCCCEEEEEECCCEEECHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCEEEEE
SEGGATSATARFMAQRENGVDLLGATSLLNLKDKDPMLAGVILDMSMNATIAASLQLGNT
ECCCCCHHHHHHHHHCCCCCEEEECEEEECCCCCCCEEEEEEEEECCCCEEEEEEHHHHH
VLSSLAGITTLHQKRVEQAGFAVLKSPDVPSILVETGFISNARDSQRLVTARHQQAVADG
HHHHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
LFEGLQRYFQKNPPVDSYMAWQQEQEGYRV
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLASAAFVLPFSVSATQIRNARLWRSDDKLRLVFDLSGSVSYKTFTLSAPERLIIDLNGA
CCCCCEEEEEECCCHHHHHCCCEECCCCCEEEEEEECCCEEEEEEEECCCCEEEEEECCC
SLIGDFSQLTLSGTPIHSIRSGHFGQGNTRIVLDLSSPVQLNSFLLAPQDGQGHRLVLDL
HHHCCCHHEEECCCCCHHHCCCCCCCCCCEEEEECCCCCEECEEEEECCCCCCCEEEEEE
MSASAGVPASVPRETPQEKSHPKRDIMVVVDPGHGGKDPGAIGAKGEREKDVVLSIAQLL
HHCCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ARRLKREKGFDVKLVRNDDFFVPLRKRVEIARQHKADMFISVHADAAPRLTASGASVYCL
HHHHHHHCCCEEEEEECCCEEECHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCEEEEE
SEGGATSATARFMAQRENGVDLLGATSLLNLKDKDPMLAGVILDMSMNATIAASLQLGNT
ECCCCCHHHHHHHHHCCCCCEEEECEEEECCCCCCCEEEEEEEEECCCCEEEEEEHHHHH
VLSSLAGITTLHQKRVEQAGFAVLKSPDVPSILVETGFISNARDSQRLVTARHQQAVADG
HHHHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
LFEGLQRYFQKNPPVDSYMAWQQEQEGYRV
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]