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The map label for this gene is yeiR [C]

Identifier: 77461875

GI number: 77461875

Start: 6336041

End: 6337246

Strand: Reverse

Name: yeiR [C]

Synonym: Pfl01_5655

Alternate gene names: 77461875

Gene position: 6337246-6336041 (Counterclockwise)

Preceding gene: 77461877

Following gene: 77461874

Centisome position: 98.43

GC content: 57.13

Gene sequence:

>1206_bases
ATGTCTTCCCGACTTCCCGTGACCGTTTTGTCCGGCTTTCTCGGTGCCGGAAAAAGTACACTTTTGAATTACGTACTACG
TAACAGAGATGATTTGCGCGTCGCCGTTATCGTCAACGATATGAGCGAGATCAACATCGATGGCAGCGAAGTCCAGCGTG
ATGTCAGCCTGAACCGTGCCGAAGAGAAGCTCGTGGAAATGAGCAACGGCTGTATCTGCTGTACGTTACGCGAAGACCTG
CTTGAAGAGGTCAGTCAGTTGGCCCGCGAAGGCCGCTTCGATTACTTGCTGATCGAATCCACGGGAATCTCCGAGCCGTT
GCCAGTCGCCGAGACGTTCACTTTCCGTGATGAACAGGGGCAAAGCCTTACCGACATCGCTCGCCTCGACACCATGGTCA
CTGTAGTCGACGGTATGAATTTCCTGCCGGACTACCAGGCCGCCGAAAACCTCGCCTCCCGTGGCGAATTTCTTGGTGAA
GAAGATGAGCGCTCGATCACCGATCTGCTGATCGAGCAGATCGAATTCGCCGACGTCCTGTTGATCAGCAAGATCGACCT
GATCAGCAGCCGCGAGCGGGAAGAGCTGATGGCCATTCTCAGACGCCTCAACGCTCAGGCCGAGATCATCCCGATGGTCA
TGGGTGAGGTACCGCTGGAGAAAATTCTCAACACCGGTCGTTTCGACTTCGAAAAAGCCGCCCAGGCCCCGGGCTGGTTG
CAGGAATTGCGTGGCGAGCATGTGCCGGAAACCGAGGAATACGGCATCGCCTCCACGGCCTATCGGGCGCGCCGGCCCTT
TCATCCACAGCGTTTCTTCGACTTCATCGATCGTCCTTGGGTAAACGGCAAATTGCTGCGCTCCAAAGGCTTTTTCTGGC
TGGCCAGCAAACCCACCGACGCCGGTAGCTGGTCCCAGGCAGGTGGCCTGATGCGCCACGGTTTCGCCGGGCGCTGGTGG
CGCTTCGTGCCAAAGGAACAGTGGCCGCAGGATCAGGAAAGTACCGCCGCGATCATGGAAAACTGGATCGCCAGCGTAGG
CGATTGTCGCCAGGAGCTGGTGTTTATCGGACAGAACATCGATTTCGCACAGCTGTCGTCGGAACTGGATGATTGCCTGC
TGACCGATGACGAAATGGCGCTCGGTACTCAAGGCTGGCTACAACTACGCGATCCGTTTGGCTCATGGCACGACGAGGTC
GCCTGA

Upstream 100 bases:

>100_bases
TTTCAAGCGTCCGTTTCCCTGGTGACCCCATCGAAGCCCAAGAACGCTCGCCTCGAGAGCCTCCTGCGCTGTTTAGCGAC
CGTCTGATGAGGTTTATCCA

Downstream 100 bases:

>100_bases
TGCTCGCCCTGACATCGCTGCCAAGCAGCATTCGTCATCAGTCTTTCGGCAAGACCCCGCAGGCACTGGCGAACATTCTC
GACGACGAGACCAACCTCGC

Product: cobalamin synthesis protein/P47K

Products: NA

Alternate protein names: P47K [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSSRLPVTVLSGFLGAGKSTLLNYVLRNRDDLRVAVIVNDMSEINIDGSEVQRDVSLNRAEEKLVEMSNGCICCTLREDL
LEEVSQLAREGRFDYLLIESTGISEPLPVAETFTFRDEQGQSLTDIARLDTMVTVVDGMNFLPDYQAAENLASRGEFLGE
EDERSITDLLIEQIEFADVLLISKIDLISSREREELMAILRRLNAQAEIIPMVMGEVPLEKILNTGRFDFEKAAQAPGWL
QELRGEHVPETEEYGIASTAYRARRPFHPQRFFDFIDRPWVNGKLLRSKGFFWLASKPTDAGSWSQAGGLMRHGFAGRWW
RFVPKEQWPQDQESTAAIMENWIASVGDCRQELVFIGQNIDFAQLSSELDDCLLTDDEMALGTQGWLQLRDPFGSWHDEV
A

Sequences:

>Translated_401_residues
MSSRLPVTVLSGFLGAGKSTLLNYVLRNRDDLRVAVIVNDMSEINIDGSEVQRDVSLNRAEEKLVEMSNGCICCTLREDL
LEEVSQLAREGRFDYLLIESTGISEPLPVAETFTFRDEQGQSLTDIARLDTMVTVVDGMNFLPDYQAAENLASRGEFLGE
EDERSITDLLIEQIEFADVLLISKIDLISSREREELMAILRRLNAQAEIIPMVMGEVPLEKILNTGRFDFEKAAQAPGWL
QELRGEHVPETEEYGIASTAYRARRPFHPQRFFDFIDRPWVNGKLLRSKGFFWLASKPTDAGSWSQAGGLMRHGFAGRWW
RFVPKEQWPQDQESTAAIMENWIASVGDCRQELVFIGQNIDFAQLSSELDDCLLTDDEMALGTQGWLQLRDPFGSWHDEV
A
>Mature_400_residues
SSRLPVTVLSGFLGAGKSTLLNYVLRNRDDLRVAVIVNDMSEINIDGSEVQRDVSLNRAEEKLVEMSNGCICCTLREDLL
EEVSQLAREGRFDYLLIESTGISEPLPVAETFTFRDEQGQSLTDIARLDTMVTVVDGMNFLPDYQAAENLASRGEFLGEE
DERSITDLLIEQIEFADVLLISKIDLISSREREELMAILRRLNAQAEIIPMVMGEVPLEKILNTGRFDFEKAAQAPGWLQ
ELRGEHVPETEEYGIASTAYRARRPFHPQRFFDFIDRPWVNGKLLRSKGFFWLASKPTDAGSWSQAGGLMRHGFAGRWWR
FVPKEQWPQDQESTAAIMENWIASVGDCRQELVFIGQNIDFAQLSSELDDCLLTDDEMALGTQGWLQLRDPFGSWHDEVA

Specific function: The presence of P47K is critical for the expression of the nitrile hydratase genes. May stabilize or activate the nitrile hydratase proteins [H]

COG id: COG0523

COG function: function code R; Putative GTPases (G3E family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cobW C-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI33469141, Length=258, Percent_Identity=31.0077519379845, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI126722884, Length=258, Percent_Identity=30.6201550387597, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI146231952, Length=258, Percent_Identity=30.6201550387597, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI148727351, Length=258, Percent_Identity=30.2325581395349, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI223941779, Length=258, Percent_Identity=30.2325581395349, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI223941776, Length=244, Percent_Identity=30.327868852459, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI119120938, Length=179, Percent_Identity=33.5195530726257, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI87082430, Length=304, Percent_Identity=26.9736842105263, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788499, Length=109, Percent_Identity=33.0275229357798, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324356, Length=234, Percent_Identity=27.3504273504274, Blast_Score=100, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR011629 [H]

Pfam domain/function: PF02492 cobW; PF07683 CobW_C [H]

EC number: NA

Molecular weight: Translated: 45438; Mature: 45307

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRLPVTVLSGFLGAGKSTLLNYVLRNRDDLRVAVIVNDMSEINIDGSEVQRDVSLNRA
CCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEECHHHCCCCHHHHHHHHHHHHH
EEKLVEMSNGCICCTLREDLLEEVSQLAREGRFDYLLIESTGISEPLPVAETFTFRDEQG
HHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHEEEECCCCC
QSLTDIARLDTMVTVVDGMNFLPDYQAAENLASRGEFLGEEDERSITDLLIEQIEFADVL
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LISKIDLISSREREELMAILRRLNAQAEIIPMVMGEVPLEKILNTGRFDFEKAAQAPGWL
HHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCCCCHHHHHCCCHHH
QELRGEHVPETEEYGIASTAYRARRPFHPQRFFDFIDRPWVNGKLLRSKGFFWLASKPTD
HHHCCCCCCCCHHCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEECCCCEEEECCCCC
AGSWSQAGGLMRHGFAGRWWRFVPKEQWPQDQESTAAIMENWIASVGDCRQELVFIGQNI
CCCCHHHCCHHHCCCCCCHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DFAQLSSELDDCLLTDDEMALGTQGWLQLRDPFGSWHDEVA
CHHHHHHHHHHHCCCCCCHHCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure 
SSRLPVTVLSGFLGAGKSTLLNYVLRNRDDLRVAVIVNDMSEINIDGSEVQRDVSLNRA
CCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEECHHHCCCCHHHHHHHHHHHHH
EEKLVEMSNGCICCTLREDLLEEVSQLAREGRFDYLLIESTGISEPLPVAETFTFRDEQG
HHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHEEEECCCCC
QSLTDIARLDTMVTVVDGMNFLPDYQAAENLASRGEFLGEEDERSITDLLIEQIEFADVL
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LISKIDLISSREREELMAILRRLNAQAEIIPMVMGEVPLEKILNTGRFDFEKAAQAPGWL
HHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCCCCHHHHHCCCHHH
QELRGEHVPETEEYGIASTAYRARRPFHPQRFFDFIDRPWVNGKLLRSKGFFWLASKPTD
HHHCCCCCCCCHHCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEECCCCEEEECCCCC
AGSWSQAGGLMRHGFAGRWWRFVPKEQWPQDQESTAAIMENWIASVGDCRQELVFIGQNI
CCCCHHHCCHHHCCCCCCHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DFAQLSSELDDCLLTDDEMALGTQGWLQLRDPFGSWHDEVA
CHHHHHHHHHHHCCCCCCHHCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2013568 [H]