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The map label for this gene is qseD [H]

Identifier: 77461845

GI number: 77461845

Start: 6297582

End: 6298493

Strand: Reverse

Name: qseD [H]

Synonym: Pfl01_5624

Alternate gene names: 77461845

Gene position: 6298493-6297582 (Counterclockwise)

Preceding gene: 77461849

Following gene: 77461843

Centisome position: 97.83

GC content: 63.82

Gene sequence:

>912_bases
ATGAATCTGGAAAGCAAATGGCTCGAGGACTTCAGTGCTCTGGCTGCCACCCGCAGCTTCTCGCAGGCGGCCGAACGGCG
CTTCGTGACCCAGCCGGCGTTCAGTCGGCGGATCCGCAGCCTGGAAGCGGCTCTCGGACTGACGCTGGTCAACCGCTCGC
GTACGCCGATCGAGCTGACGGCGGCGGGACAACTGTTCCTGGTGACCGCGCGCACCGTGGTCGAACAGCTCGGTGAGGTG
TTGCGCCATTTGCATCACCTCGAAGGTGGGCAAGGCGAAGTGATCCAGGTCGCAGCGGCGCATTCGTTGGCGCTCGGATT
CTTCCCGCGCTGGATCGCGCAACTGCGCAACGAAGGCCTGAACATCGCTACCCGGCTGGTCGCGACCAACGTTGGCGACG
CGGTGCACGCGCTGCGTGAAGGTGGTTGCGATCTGATGCTGGCGTTCTACGACCCGGACGCCGCCATGCAGATGGACCCG
GAAATCTTCCCGTCGCTGCACCTGGGCCAGACCGAAATGCTTCCTGTGTGTGCGGCAGATGCCAATGGCAAACCGTTGTT
CGATCTGGAAGGCGAGGGCAGTGTGCCGTTGCTGGCGTACAGCGCCGGGGCGTTTCTCGGGCGTTCGGTGAATGGCTTGC
TGCGCCAGCGTCAGCTGCGCTTCACCACAATCTATGAAACCGCCATGGCCGACAGCTTGAAAAGCATGGCGCTGGAAGGT
CTCGGCATTGCCTGGGTGCCGCAACTCAGCGTGCGGGCTGAACTGGCCCGCGGTGAACTGGTGGTTTGCGGCGGCTCGCA
ATGGCATGTGCCGCTGGAGATTCGCCTGTACCGCTGCGCGCTGGTGCGCAAGGCCAACGTGCGGTTGTTGTGGCGCAAGC
TTGAAGGTGGTGCGGCGGGCTCCTCATCTTGA

Upstream 100 bases:

>100_bases
CTGTTAAACACTGCCCTATGCACATTCGGCATAATGCCCGTGTGACGCCGCGTGCGCGGCAGACGTGTGACCTGTTTTGG
TGCGTGTCCGGGAGGACGTG

Downstream 100 bases:

>100_bases
TGGAGCACTGATAGCGCGTAACGGAAAGCCGGGAATGAAGCAGGCGTGTATCTGCTTCAGCCCTCTTCATCATCATATTC
GGGGCGCGGGACTGCTCGTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Quorum-sensing regulator protein D [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNLESKWLEDFSALAATRSFSQAAERRFVTQPAFSRRIRSLEAALGLTLVNRSRTPIELTAAGQLFLVTARTVVEQLGEV
LRHLHHLEGGQGEVIQVAAAHSLALGFFPRWIAQLRNEGLNIATRLVATNVGDAVHALREGGCDLMLAFYDPDAAMQMDP
EIFPSLHLGQTEMLPVCAADANGKPLFDLEGEGSVPLLAYSAGAFLGRSVNGLLRQRQLRFTTIYETAMADSLKSMALEG
LGIAWVPQLSVRAELARGELVVCGGSQWHVPLEIRLYRCALVRKANVRLLWRKLEGGAAGSSS

Sequences:

>Translated_303_residues
MNLESKWLEDFSALAATRSFSQAAERRFVTQPAFSRRIRSLEAALGLTLVNRSRTPIELTAAGQLFLVTARTVVEQLGEV
LRHLHHLEGGQGEVIQVAAAHSLALGFFPRWIAQLRNEGLNIATRLVATNVGDAVHALREGGCDLMLAFYDPDAAMQMDP
EIFPSLHLGQTEMLPVCAADANGKPLFDLEGEGSVPLLAYSAGAFLGRSVNGLLRQRQLRFTTIYETAMADSLKSMALEG
LGIAWVPQLSVRAELARGELVVCGGSQWHVPLEIRLYRCALVRKANVRLLWRKLEGGAAGSSS
>Mature_303_residues
MNLESKWLEDFSALAATRSFSQAAERRFVTQPAFSRRIRSLEAALGLTLVNRSRTPIELTAAGQLFLVTARTVVEQLGEV
LRHLHHLEGGQGEVIQVAAAHSLALGFFPRWIAQLRNEGLNIATRLVATNVGDAVHALREGGCDLMLAFYDPDAAMQMDP
EIFPSLHLGQTEMLPVCAADANGKPLFDLEGEGSVPLLAYSAGAFLGRSVNGLLRQRQLRFTTIYETAMADSLKSMALEG
LGIAWVPQLSVRAELARGELVVCGGSQWHVPLEIRLYRCALVRKANVRLLWRKLEGGAAGSSS

Specific function: Down-regulates expression of the flagellar regulon, decreasing motility [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790783, Length=297, Percent_Identity=38.3838383838384, Blast_Score=200, Evalue=7e-53,
Organism=Escherichia coli, GI145693105, Length=256, Percent_Identity=23.4375, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081904, Length=159, Percent_Identity=27.6729559748428, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790399, Length=270, Percent_Identity=27.037037037037, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33016; Mature: 33016

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS50931 HTH_LYSR ; PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLESKWLEDFSALAATRSFSQAAERRFVTQPAFSRRIRSLEAALGLTLVNRSRTPIELT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCCCEEEE
AAGQLFLVTARTVVEQLGEVLRHLHHLEGGQGEVIQVAAAHSLALGFFPRWIAQLRNEGL
ECCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCC
NIATRLVATNVGDAVHALREGGCDLMLAFYDPDAAMQMDPEIFPSLHLGQTEMLPVCAAD
CHHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCCCCCCCCEEEECC
ANGKPLFDLEGEGSVPLLAYSAGAFLGRSVNGLLRQRQLRFTTIYETAMADSLKSMALEG
CCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LGIAWVPQLSVRAELARGELVVCGGSQWHVPLEIRLYRCALVRKANVRLLWRKLEGGAAG
CCEEECCCHHHHHHHCCCCEEEECCCCEEEEHEEEEEHHHHHHHCCCEEEEEECCCCCCC
SSS
CCC
>Mature Secondary Structure
MNLESKWLEDFSALAATRSFSQAAERRFVTQPAFSRRIRSLEAALGLTLVNRSRTPIELT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCCCEEEE
AAGQLFLVTARTVVEQLGEVLRHLHHLEGGQGEVIQVAAAHSLALGFFPRWIAQLRNEGL
ECCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCC
NIATRLVATNVGDAVHALREGGCDLMLAFYDPDAAMQMDPEIFPSLHLGQTEMLPVCAAD
CHHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCCCCCCCCEEEECC
ANGKPLFDLEGEGSVPLLAYSAGAFLGRSVNGLLRQRQLRFTTIYETAMADSLKSMALEG
CCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LGIAWVPQLSVRAELARGELVVCGGSQWHVPLEIRLYRCALVRKANVRLLWRKLEGGAAG
CCEEECCCHHHHHHHCCCCEEEECCCCEEEEHEEEEEHHHHHHHCCCEEEEEECCCCCCC
SSS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7876157 [H]