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The map label for this gene is 77461800

Identifier: 77461800

GI number: 77461800

Start: 6250779

End: 6252110

Strand: Reverse

Name: 77461800

Synonym: Pfl01_5579

Alternate gene names: NA

Gene position: 6252110-6250779 (Counterclockwise)

Preceding gene: 77461801

Following gene: 77461799

Centisome position: 97.11

GC content: 61.71

Gene sequence:

>1332_bases
ATGAATACCCATAAAGTCATCTGGCAGGAAGGCATGCTGCTGCGCCCGCAGCACTTCCAGCACAACGACCGCTACTACGA
TCACCAGATGAAGACCCGCACCCAGTTGCTGGGCGGCTACACCTGGGGTTTCCTGAATCTGGAGATCGACCTGCAGTTCC
TCAACATGGGCAAACTGGTGATCAGTGAAGCCTCGGGGATCCTGCCGGACGGCAGCCTGTTCGAACTCGGTGGCAACACC
GAGCCGCTGGCGCTGGACGTACCGCCGAACACCGGCAACATGCCGATCTACCTGGCGCTGCCGCTGGTCACCGGTAACCA
CATCGAGGCCCGGCGCCCGGAGCAGTCCGATGTGCTGGCGCGCTACACCGCATATGACGCCGAAGTCGCCGACTCCAACG
CCGGTGATGATTCCGCCAGCCAGGTCAGTTGCGGCCGCCCGGACTTCAAGCTGTTGCTCGGCGAGCAGCAGAGTGACCAG
GCTTACGTGAAGCTGAAGATCTGCGATGTGCTCGACACCACGCCGGACGGCGTGATCAGCCTCGATCCGGATTTCGTGCC
GACCTACATTCAGGCCCACGCCTCCAGCTACCTGCTGTCGTGCCTGAAAGAAGTGATCAGCATGCTCGGCCACCGGGGCG
ACACCATTGCCGAGCGCATTCGTTCGAACGGCAAGGTCGGCGGCGCGGAAATCGGCGACTTCATGATGCTGCAACTGATA
AACCGCACCGAACTGCTGCTGCGCCACTACCTGGGCCTGGAGCAGGTTCACCCGGAAGAGTTGTATCGTACGCTGCTGAC
CATGCTCGGCGATCTGGCGACGTTCTCCAGCGACAGCAAACGCCCGCGTCTGGACAGCCGCTACTCCCACGCCGATCAGG
GCGCGAGCTTCCGCAAACTGATGGAGTCGATCCGTCAGGTGCTGTCGATGGTGCTGGAACAGCACGCCATCGAACTGATC
CTGCAAGCGCGTCAGTACGGGATCATCGTGTCGCCGCTGCACGACCACAAACTGCTGGGCTCGGCGTCGTTCGTGCTGGC
AGCCAGTGCCAACTGCGACTCCGAAGAACTGCGCAACCGCTTGCCTGCGCACCTCAAGGTCGGCCCGGTGGAGCGCATCC
GCCAACTGGTCAACCTGCACCTGCCGGGGATCAAGGTCAAACCGTTGCCGGTGGCCCCGCGGCAGATCGCGTTCCACTCC
AACAAAACCTATTTCATCCTCGAACTCAGTTCCGAAGACCTGGCACAACTCGAGCGCTCCGGCGGCTTCGCGTTCCATGT
GTCCGGCGAATTCGCCGAGCTTGAACTGAAATTCTGGGCCATCAGGAACTGA

Upstream 100 bases:

>100_bases
TCCCGATCACACCGCTGGAAGTCACCGAAGCCGATCTGACCCTGGATCAGGCCGGTATCCGCAAGACCAACGAAGTGCTC
GCCAAGGCGGATGACTGACC

Downstream 100 bases:

>100_bases
CCGACATGATCAAGGACATGGAACACAACCAGGACGACAAAACCGTCCTGCTCGACCGTCAGGGCCATGGCCCTGCGGCG
AGTCCGCTGACCGACTTCGC

Product: hypothetical protein

Products: NA

Alternate protein names: Type VI Secretion Protein Family; Protein ImpJ/VasE; Conserved Protein; Replication/Virulence Associated Protein; Protein ImpJ; Type VI Secretion System Protein; Type VI Secretion System Protein ImpJ; Type VI Secretion; Type VI Secretion System Core Protein; T6ss M; Type VI Secretion Protein VasE; Temperature-Dependent Protein Secretion ImpJ

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MNTHKVIWQEGMLLRPQHFQHNDRYYDHQMKTRTQLLGGYTWGFLNLEIDLQFLNMGKLVISEASGILPDGSLFELGGNT
EPLALDVPPNTGNMPIYLALPLVTGNHIEARRPEQSDVLARYTAYDAEVADSNAGDDSASQVSCGRPDFKLLLGEQQSDQ
AYVKLKICDVLDTTPDGVISLDPDFVPTYIQAHASSYLLSCLKEVISMLGHRGDTIAERIRSNGKVGGAEIGDFMMLQLI
NRTELLLRHYLGLEQVHPEELYRTLLTMLGDLATFSSDSKRPRLDSRYSHADQGASFRKLMESIRQVLSMVLEQHAIELI
LQARQYGIIVSPLHDHKLLGSASFVLAASANCDSEELRNRLPAHLKVGPVERIRQLVNLHLPGIKVKPLPVAPRQIAFHS
NKTYFILELSSEDLAQLERSGGFAFHVSGEFAELELKFWAIRN

Sequences:

>Translated_443_residues
MNTHKVIWQEGMLLRPQHFQHNDRYYDHQMKTRTQLLGGYTWGFLNLEIDLQFLNMGKLVISEASGILPDGSLFELGGNT
EPLALDVPPNTGNMPIYLALPLVTGNHIEARRPEQSDVLARYTAYDAEVADSNAGDDSASQVSCGRPDFKLLLGEQQSDQ
AYVKLKICDVLDTTPDGVISLDPDFVPTYIQAHASSYLLSCLKEVISMLGHRGDTIAERIRSNGKVGGAEIGDFMMLQLI
NRTELLLRHYLGLEQVHPEELYRTLLTMLGDLATFSSDSKRPRLDSRYSHADQGASFRKLMESIRQVLSMVLEQHAIELI
LQARQYGIIVSPLHDHKLLGSASFVLAASANCDSEELRNRLPAHLKVGPVERIRQLVNLHLPGIKVKPLPVAPRQIAFHS
NKTYFILELSSEDLAQLERSGGFAFHVSGEFAELELKFWAIRN
>Mature_443_residues
MNTHKVIWQEGMLLRPQHFQHNDRYYDHQMKTRTQLLGGYTWGFLNLEIDLQFLNMGKLVISEASGILPDGSLFELGGNT
EPLALDVPPNTGNMPIYLALPLVTGNHIEARRPEQSDVLARYTAYDAEVADSNAGDDSASQVSCGRPDFKLLLGEQQSDQ
AYVKLKICDVLDTTPDGVISLDPDFVPTYIQAHASSYLLSCLKEVISMLGHRGDTIAERIRSNGKVGGAEIGDFMMLQLI
NRTELLLRHYLGLEQVHPEELYRTLLTMLGDLATFSSDSKRPRLDSRYSHADQGASFRKLMESIRQVLSMVLEQHAIELI
LQARQYGIIVSPLHDHKLLGSASFVLAASANCDSEELRNRLPAHLKVGPVERIRQLVNLHLPGIKVKPLPVAPRQIAFHS
NKTYFILELSSEDLAQLERSGGFAFHVSGEFAELELKFWAIRN

Specific function: Unknown

COG id: COG3522

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 49595; Mature: 49595

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTHKVIWQEGMLLRPQHFQHNDRYYDHQMKTRTQLLGGYTWGFLNLEIDLQFLNMGKLV
CCCCCEEECCCCEECCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECCCCEE
ISEASGILPDGSLFELGGNTEPLALDVPPNTGNMPIYLALPLVTGNHIEARRPEQSDVLA
EECCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHH
RYTAYDAEVADSNAGDDSASQVSCGRPDFKLLLGEQQSDQAYVKLKICDVLDTTPDGVIS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEHHCCCCCCEEE
LDPDFVPTYIQAHASSYLLSCLKEVISMLGHRGDTIAERIRSNGKVGGAEIGDFMMLQLI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHH
NRTELLLRHYLGLEQVHPEELYRTLLTMLGDLATFSSDSKRPRLDSRYSHADQGASFRKL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCHHHHHH
MESIRQVLSMVLEQHAIELILQARQYGIIVSPLHDHKLLGSASFVLAASANCDSEELRNR
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHCCCEEEEEECCCCCHHHHHHH
LPAHLKVGPVERIRQLVNLHLPGIKVKPLPVAPRQIAFHSNKTYFILELSSEDLAQLERS
CCCCEEECHHHHHHHHHHCCCCCEEEECCCCCCHHEEEECCCEEEEEEECCHHHHHHHHC
GGFAFHVSGEFAELELKFWAIRN
CCEEEEECCCEEEEEEEEEEECC
>Mature Secondary Structure
MNTHKVIWQEGMLLRPQHFQHNDRYYDHQMKTRTQLLGGYTWGFLNLEIDLQFLNMGKLV
CCCCCEEECCCCEECCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECCCCEE
ISEASGILPDGSLFELGGNTEPLALDVPPNTGNMPIYLALPLVTGNHIEARRPEQSDVLA
EECCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHH
RYTAYDAEVADSNAGDDSASQVSCGRPDFKLLLGEQQSDQAYVKLKICDVLDTTPDGVIS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEHHCCCCCCEEE
LDPDFVPTYIQAHASSYLLSCLKEVISMLGHRGDTIAERIRSNGKVGGAEIGDFMMLQLI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHH
NRTELLLRHYLGLEQVHPEELYRTLLTMLGDLATFSSDSKRPRLDSRYSHADQGASFRKL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCHHHHHH
MESIRQVLSMVLEQHAIELILQARQYGIIVSPLHDHKLLGSASFVLAASANCDSEELRNR
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHCCCEEEEEECCCCCHHHHHHH
LPAHLKVGPVERIRQLVNLHLPGIKVKPLPVAPRQIAFHSNKTYFILELSSEDLAQLERS
CCCCEEECHHHHHHHHHHCCCCCEEEECCCCCCHHEEEECCCEEEEEEECCHHHHHHHHC
GGFAFHVSGEFAELELKFWAIRN
CCEEEEECCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA