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The map label for this gene is gcvA [H]

Identifier: 77461737

GI number: 77461737

Start: 6183110

End: 6184030

Strand: Reverse

Name: gcvA [H]

Synonym: Pfl01_5516

Alternate gene names: 77461737

Gene position: 6184030-6183110 (Counterclockwise)

Preceding gene: 77461739

Following gene: 77461736

Centisome position: 96.05

GC content: 64.6

Gene sequence:

>921_bases
ATGTCCCGTCGTCTTCCACCCTTGTATGCCCTGCGCGCATTCGAAGCGGCGGCCCGCCACAGTTCGTTCACCCGGGCGGC
TGAAGAATTGTCGATTACCCAGAGTGCGGTGAGCCGGCACATCCGTACGCTCGAAGAGCACTTCGCCTGCCGCTTGTTCC
ACCGCAGCGGGCGCAACCTGCAACTGACCGAGTCGGCGCGCCTGCTGCTGCCGGGCATCCGCGAAGGCTTCGCCGCGCTG
GAGCGAGCCTGCAATACCTTGCGAGCCGAAGATGACATCCTGCGCATGAAGGCCCCGTCGACCCTGACCATGCGCTGGTT
GCTGGCGCGGCTCAGTCGCTTCCGCCATTTGCAGCCCGGCAATGAGGTGCAACTGACCAGTGCGTGGATGGATGTGGATT
CGGTGGACTTCAACAACGAGCCCTTCGATTGCGCAGTATTGCTGAGCGACGGGCATTTTCCGCTGGACTGGGAAGCCAGT
CTGCTGTTTCCCGAAGAGCTGATTCCGGTCGGCGCGCCGAACCTGCTCAACGATCAGCCCTGGGATGTGGCGCGACTGGC
CAGCGCCGAGTTGCTGCACCCGACGCCGGATCGCCGGGACTGGCGTAGCTGGCTGGAGCACATGGGGCTGTCGGATCAGG
TGTCGCTCAAGGGCGGGCAGGTGTTCGATACGCTGGAACTGGGCATGATCGCGGCGGCCCGGGGCTATGGGGTCTCCATG
GGCGATCTGCTGATGGTGGCCGAGGATGTCGCGCAAGGGCGCCTGAGTCTGCCTTGGCCGACGGCGGTCGCCAGCGGACT
GAATTATTACCTGGTGTGGCCAAAGACCCGTCCGGGAGGTGAACGTTTGCGCCGCCTCAGCGACTTCCTGCAAAACGAAG
TGCGCGCCATGGCGCTGCCGGCGGTCACGCGCTTGAGCTGA

Upstream 100 bases:

>100_bases
CAGGTTGCACAAAGGGATAAAAAGGATGGGTGGCATGATTAAAACTCATCCTGCCGCGATTCTGCTAAGTTGACGCAATC
CCGCCATTGGAGCGTTGTGC

Downstream 100 bases:

>100_bases
AACGTTCGAGCCTGCACAATGCCGGCCTTTCGCTATACCCCTGCTGATCGCTCAAGTATTCGGTTTCACCGCCGAATACG
TGCATGTGGGACCGTCGTGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSRRLPPLYALRAFEAAARHSSFTRAAEELSITQSAVSRHIRTLEEHFACRLFHRSGRNLQLTESARLLLPGIREGFAAL
ERACNTLRAEDDILRMKAPSTLTMRWLLARLSRFRHLQPGNEVQLTSAWMDVDSVDFNNEPFDCAVLLSDGHFPLDWEAS
LLFPEELIPVGAPNLLNDQPWDVARLASAELLHPTPDRRDWRSWLEHMGLSDQVSLKGGQVFDTLELGMIAAARGYGVSM
GDLLMVAEDVAQGRLSLPWPTAVASGLNYYLVWPKTRPGGERLRRLSDFLQNEVRAMALPAVTRLS

Sequences:

>Translated_306_residues
MSRRLPPLYALRAFEAAARHSSFTRAAEELSITQSAVSRHIRTLEEHFACRLFHRSGRNLQLTESARLLLPGIREGFAAL
ERACNTLRAEDDILRMKAPSTLTMRWLLARLSRFRHLQPGNEVQLTSAWMDVDSVDFNNEPFDCAVLLSDGHFPLDWEAS
LLFPEELIPVGAPNLLNDQPWDVARLASAELLHPTPDRRDWRSWLEHMGLSDQVSLKGGQVFDTLELGMIAAARGYGVSM
GDLLMVAEDVAQGRLSLPWPTAVASGLNYYLVWPKTRPGGERLRRLSDFLQNEVRAMALPAVTRLS
>Mature_305_residues
SRRLPPLYALRAFEAAARHSSFTRAAEELSITQSAVSRHIRTLEEHFACRLFHRSGRNLQLTESARLLLPGIREGFAALE
RACNTLRAEDDILRMKAPSTLTMRWLLARLSRFRHLQPGNEVQLTSAWMDVDSVDFNNEPFDCAVLLSDGHFPLDWEASL
LFPEELIPVGAPNLLNDQPWDVARLASAELLHPTPDRRDWRSWLEHMGLSDQVSLKGGQVFDTLELGMIAAARGYGVSMG
DLLMVAEDVAQGRLSLPWPTAVASGLNYYLVWPKTRPGGERLRRLSDFLQNEVRAMALPAVTRLS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=276, Percent_Identity=35.8695652173913, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=30.0341296928328, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1788706, Length=302, Percent_Identity=30.4635761589404, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693193, Length=306, Percent_Identity=26.4705882352941, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787589, Length=294, Percent_Identity=26.8707482993197, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787128, Length=141, Percent_Identity=31.2056737588652, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI157672245, Length=226, Percent_Identity=27.4336283185841, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34424; Mature: 34293

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLPPLYALRAFEAAARHSSFTRAAEELSITQSAVSRHIRTLEEHFACRLFHRSGRNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
QLTESARLLLPGIREGFAALERACNTLRAEDDILRMKAPSTLTMRWLLARLSRFRHLQPG
EEECCCHHHCCHHHHHHHHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHHHHHCCCCC
NEVQLTSAWMDVDSVDFNNEPFDCAVLLSDGHFPLDWEASLLFPEELIPVGAPNLLNDQP
CCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECHHHHCCCCCCCCCCCCC
WDVARLASAELLHPTPDRRDWRSWLEHMGLSDQVSLKGGQVFDTLELGMIAAARGYGVSM
CHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCH
GDLLMVAEDVAQGRLSLPWPTAVASGLNYYLVWPKTRPGGERLRRLSDFLQNEVRAMALP
HHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
AVTRLS
HHHHCC
>Mature Secondary Structure 
SRRLPPLYALRAFEAAARHSSFTRAAEELSITQSAVSRHIRTLEEHFACRLFHRSGRNL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
QLTESARLLLPGIREGFAALERACNTLRAEDDILRMKAPSTLTMRWLLARLSRFRHLQPG
EEECCCHHHCCHHHHHHHHHHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHHHHHCCCCC
NEVQLTSAWMDVDSVDFNNEPFDCAVLLSDGHFPLDWEASLLFPEELIPVGAPNLLNDQP
CCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECHHHHCCCCCCCCCCCCC
WDVARLASAELLHPTPDRRDWRSWLEHMGLSDQVSLKGGQVFDTLELGMIAAARGYGVSM
CHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCH
GDLLMVAEDVAQGRLSLPWPTAVASGLNYYLVWPKTRPGGERLRRLSDFLQNEVRAMALP
HHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
AVTRLS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]