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The map label for this gene is pcpB [H]

Identifier: 77459972

GI number: 77459972

Start: 4237892

End: 4239427

Strand: Direct

Name: pcpB [H]

Synonym: Pfl01_3750

Alternate gene names: 77459972

Gene position: 4237892-4239427 (Clockwise)

Preceding gene: 77459970

Following gene: 77459975

Centisome position: 65.82

GC content: 63.35

Gene sequence:

>1536_bases
ATGAACCGCAGTGATGTTCTGATCATCGGCGCGGGCCCCACCGGGCTGGTGCTGGCCCTGTGGCTGAGCAAACTGGGCGT
GCGGGTGCGCATCATCGACAAGGCTTCCGCGCCGGGCACCACTTCAAGGGCGCTGGCGGTGCAGGCGCGCACCCTTGAGC
TGTACCGGCAACTGGATCTGGCCGACGCGATAGTGCGTAACGGTCACCGGGTGGCGGCGGCCAATTTCTGGGTCAAGGGC
AAACCTGTGGCACAACTGCCGCTGAACCGGATCGGCGAAGGCCTGACGCCCTATGCCTTTCTGGAAATGTACCCGCAGGA
CGAACACGAGCGACTGTTGATCGACCGCCTCGAAGACTACGACGTGACCGTCGAGCGCAACACGACGCTGGAGAGTTTCA
CCGAAAACGGCGACGGCCTCACCGCGCATTTGCGCCTGCCGGATGGCGAGCAGGAAATCTGCCAGGCCTGCTATCTGGCC
GGGTGTGACGGCGCCCGTTCGGTCGTGCGCAAAACCCTCGACACCGGTTTTCCCGGCGGCACTTATCAGCAGATTTTCTA
TGTCGCGGATGTGCAGGCCAGCGGCCCGGCGCTCAACGGCGAGTTGCATCTGGATCTGGATGAGGCGGACTTTCTCGCGG
TGTTCCCGCTGGCCGGCGAAGGCCGTGCGCGGTTGATCGGCACGGTGCGTGACGAACGTGCGGATCGCGCCGAAACCCTC
GAGTTTTCCGATGTCAGCAATCGCGCCATCGAACATCTGAACGTGCACATCGAGGACGTCAACTGGTTCTCCACCTACCG
CGTGCATCATCGGGTGGCGGATCACTTTCGCCAGGGCCGGGCGTTTCTGCTCGGCGATGCGGCGCATGTGCACAGCCCGG
CCGGCGGTCAGGGCATGAACACCGGCATCGGCGATGCGATCAATCTGGCGTGGAAACTCGCGGCAGTGCTCGGCGGCGCA
GCCACGCCCCAACTGCTCGACAGCTACGAAACCGAACGCATCGCCTTCGCCCGGCGACTGGTGTCGACCACCGACAAGGT
CTTCAGCTTCGTCACCGCCGAAGGCCGAATGGCCGACCTGTTGCGCACGCGGCTGGCACCGTTCCTGATTCCGAAAATGG
CCTCGTTCGAAACCAGCCGCGAATTCCTGTTTCGCACGGTGTCGCAGATCACCCTCAACTATCGGGGCATGGCGTTGAGT
GAAGGCGTGGCCGGGCACGTCCACGGCGGTGACCGCTTGCCGTGGGCTCATGATGGCGAGGGGGACAATTACGAACCGTT
GCGCCATCCGTGCTGGCAGGTGCATGTGTACGGCGACACCAGCGACGAGATGATCGCCTGGTGCAATGAACATCACTTGC
CGCTGCACGTGTTCGACTGGCGCCCGGCGTTTGAAACGGCGGGACTGGGGCGCAACGGCTTTTACCTGCTGCGCCCGGAT
ACCTACGTGGCGATTGCCGACAACAGCGCGGATCCGAAGGTGATCGAACGGTATTTCCGTGATCACGGGATCCGGCCGTT
TTTCAATTGCCCCTGA

Upstream 100 bases:

>100_bases
CGTTTGGCAGGCCGACTGGCCGCCCTCACCGTCTGATCCATACTTGCTGCTGCACACTCAGTGGCATGCCGCAGGTCAGG
ACACGCGAGGAGGACAGACC

Downstream 100 bases:

>100_bases
AGGATCAGATCCCGGCCAGGGCCAGGTCCATCGCAAAGTAGGTGAAGATCAGATCCGCACCCGCGCGTTTGATCGCACCG
AGGCTTTCGCGCACCACGCG

Product: monooxygenase

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MNRSDVLIIGAGPTGLVLALWLSKLGVRVRIIDKASAPGTTSRALAVQARTLELYRQLDLADAIVRNGHRVAAANFWVKG
KPVAQLPLNRIGEGLTPYAFLEMYPQDEHERLLIDRLEDYDVTVERNTTLESFTENGDGLTAHLRLPDGEQEICQACYLA
GCDGARSVVRKTLDTGFPGGTYQQIFYVADVQASGPALNGELHLDLDEADFLAVFPLAGEGRARLIGTVRDERADRAETL
EFSDVSNRAIEHLNVHIEDVNWFSTYRVHHRVADHFRQGRAFLLGDAAHVHSPAGGQGMNTGIGDAINLAWKLAAVLGGA
ATPQLLDSYETERIAFARRLVSTTDKVFSFVTAEGRMADLLRTRLAPFLIPKMASFETSREFLFRTVSQITLNYRGMALS
EGVAGHVHGGDRLPWAHDGEGDNYEPLRHPCWQVHVYGDTSDEMIAWCNEHHLPLHVFDWRPAFETAGLGRNGFYLLRPD
TYVAIADNSADPKVIERYFRDHGIRPFFNCP

Sequences:

>Translated_511_residues
MNRSDVLIIGAGPTGLVLALWLSKLGVRVRIIDKASAPGTTSRALAVQARTLELYRQLDLADAIVRNGHRVAAANFWVKG
KPVAQLPLNRIGEGLTPYAFLEMYPQDEHERLLIDRLEDYDVTVERNTTLESFTENGDGLTAHLRLPDGEQEICQACYLA
GCDGARSVVRKTLDTGFPGGTYQQIFYVADVQASGPALNGELHLDLDEADFLAVFPLAGEGRARLIGTVRDERADRAETL
EFSDVSNRAIEHLNVHIEDVNWFSTYRVHHRVADHFRQGRAFLLGDAAHVHSPAGGQGMNTGIGDAINLAWKLAAVLGGA
ATPQLLDSYETERIAFARRLVSTTDKVFSFVTAEGRMADLLRTRLAPFLIPKMASFETSREFLFRTVSQITLNYRGMALS
EGVAGHVHGGDRLPWAHDGEGDNYEPLRHPCWQVHVYGDTSDEMIAWCNEHHLPLHVFDWRPAFETAGLGRNGFYLLRPD
TYVAIADNSADPKVIERYFRDHGIRPFFNCP
>Mature_511_residues
MNRSDVLIIGAGPTGLVLALWLSKLGVRVRIIDKASAPGTTSRALAVQARTLELYRQLDLADAIVRNGHRVAAANFWVKG
KPVAQLPLNRIGEGLTPYAFLEMYPQDEHERLLIDRLEDYDVTVERNTTLESFTENGDGLTAHLRLPDGEQEICQACYLA
GCDGARSVVRKTLDTGFPGGTYQQIFYVADVQASGPALNGELHLDLDEADFLAVFPLAGEGRARLIGTVRDERADRAETL
EFSDVSNRAIEHLNVHIEDVNWFSTYRVHHRVADHFRQGRAFLLGDAAHVHSPAGGQGMNTGIGDAINLAWKLAAVLGGA
ATPQLLDSYETERIAFARRLVSTTDKVFSFVTAEGRMADLLRTRLAPFLIPKMASFETSREFLFRTVSQITLNYRGMALS
EGVAGHVHGGDRLPWAHDGEGDNYEPLRHPCWQVHVYGDTSDEMIAWCNEHHLPLHVFDWRPAFETAGLGRNGFYLLRPD
TYVAIADNSADPKVIERYFRDHGIRPFFNCP

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=409, Percent_Identity=28.8508557457213, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 56801; Mature: 56801

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSDVLIIGAGPTGLVLALWLSKLGVRVRIIDKASAPGTTSRALAVQARTLELYRQLDL
CCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHH
ADAIVRNGHRVAAANFWVKGKPVAQLPLNRIGEGLTPYAFLEMYPQDEHERLLIDRLEDY
HHHHHHCCCEEEEEEEEECCCCHHCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCC
DVTVERNTTLESFTENGDGLTAHLRLPDGEQEICQACYLAGCDGARSVVRKTLDTGFPGG
EEEEECCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
TYQQIFYVADVQASGPALNGELHLDLDEADFLAVFPLAGEGRARLIGTVRDERADRAETL
CCEEEEEEEEECCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEEEEEHHHHCCCHHHEE
EFSDVSNRAIEHLNVHIEDVNWFSTYRVHHRVADHFRQGRAFLLGDAAHVHSPAGGQGMN
ECCHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCCCCCCCC
TGIGDAINLAWKLAAVLGGAATPQLLDSYETERIAFARRLVSTTDKVFSFVTAEGRMADL
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LRTRLAPFLIPKMASFETSREFLFRTVSQITLNYRGMALSEGVAGHVHGGDRLPWAHDGE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECCEEECCCCEEECCCCCCCCCCCCCC
GDNYEPLRHPCWQVHVYGDTSDEMIAWCNEHHLPLHVFDWRPAFETAGLGRNGFYLLRPD
CCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCEEEECCC
TYVAIADNSADPKVIERYFRDHGIRPFFNCP
EEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNRSDVLIIGAGPTGLVLALWLSKLGVRVRIIDKASAPGTTSRALAVQARTLELYRQLDL
CCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHH
ADAIVRNGHRVAAANFWVKGKPVAQLPLNRIGEGLTPYAFLEMYPQDEHERLLIDRLEDY
HHHHHHCCCEEEEEEEEECCCCHHCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCC
DVTVERNTTLESFTENGDGLTAHLRLPDGEQEICQACYLAGCDGARSVVRKTLDTGFPGG
EEEEECCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
TYQQIFYVADVQASGPALNGELHLDLDEADFLAVFPLAGEGRARLIGTVRDERADRAETL
CCEEEEEEEEECCCCCCCCCEEEEEECCCCEEEEEEECCCCCEEEEEEHHHHCCCHHHEE
EFSDVSNRAIEHLNVHIEDVNWFSTYRVHHRVADHFRQGRAFLLGDAAHVHSPAGGQGMN
ECCHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCCCCCCCC
TGIGDAINLAWKLAAVLGGAATPQLLDSYETERIAFARRLVSTTDKVFSFVTAEGRMADL
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LRTRLAPFLIPKMASFETSREFLFRTVSQITLNYRGMALSEGVAGHVHGGDRLPWAHDGE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECCEEECCCCEEECCCCCCCCCCCCCC
GDNYEPLRHPCWQVHVYGDTSDEMIAWCNEHHLPLHVFDWRPAFETAGLGRNGFYLLRPD
CCCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCEEEECCC
TYVAIADNSADPKVIERYFRDHGIRPFFNCP
EEEEEECCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]