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The map label for this gene is tig [H]

Identifier: 77459920

GI number: 77459920

Start: 4186496

End: 4187806

Strand: Reverse

Name: tig [H]

Synonym: Pfl01_3698

Alternate gene names: 77459920

Gene position: 4187806-4186496 (Counterclockwise)

Preceding gene: 77459923

Following gene: 77459919

Centisome position: 65.04

GC content: 56.29

Gene sequence:

>1311_bases
ATGCAAGTTTCTGTTGAAAATACTACTGCTCTTGAGCGCCGCATGAGCATCACCGTGCCGGCTGAGCGCATCGAGACTCA
GGTCAACAAGCGTCTGCAGCAGACTGCCCAAAAGGCCAAGATTGCTGGCTTCCGTCCAGGCAAAGTGCCAATGAGCGAAA
TCAAGCGCCGTTTCGGTGCTGATGCGCGCCAGGAAGCGATCGGCGACGTGATCCAGTCCTCCTTCTACGAAGCTGTGGTT
GAGCAGAAGCTGAACCCGGCCGGTTCGCCATCGATCGAGCCTAAGTCGCTCGAAGCTGGCAAGGACCTGGAATACGTAGC
CGTATTCGAAGTGTTCCCTGAGTTCACCGTTGCCGGCTTCGAAGGTATCACCGTCGAGCGTCTGAGCGCTGACGTGGCTG
ACGCCGATCTGGATAAGATGCTGGACATCCTGCGCAAGCAGAACACCCGTTTCGAAGTGGCCGATCGCGCTGCCCAGAAC
GAAGACCAGCTGAATATCGATTTCGTTGGCAAGGTTGATGGCGAAGTATTCGCTGGCGGTTCCGCCAAGGGCACTCAGCT
GGTGCTGGGTTCCGGCCGCATGATCCCAGGCTTCGAAGAAGGCCTGGTCGGCGCTAAAGCCGGCGAAGAGCGCGTTCTGA
ACCTGACCTTCCCAGAGGACTATCAGAACCTGGACCTGGCTGGCAAAACCGCCGAGTTCACCGTGACTGTAAACACCGTT
TCCGAGCCAAAACTGCCTGAGCTGAACGAAGAGTTCTTCGCTCAATTCGGCATCAAGGAAAGCGGCATCGACGGTTTCCG
CACCGAAGTTCGCAAGAACATGGAGCGTGAACTGCGTCAGGCGATCAAATCCAAGGTCAAGAATCAGGTAATGGACGGTC
TGCTGGCCACCAACCCGATCGAAGTGCCGAAGGCTCTGCTGTCCAACGAAGTTGACCGTCTGCGCGTGCAGGCTGTTCAG
CAGTTCGGTGGCAACATCAAGCCTGACCAACTGCCGGCCGAGCTGTTCGAAGAGCAAGCCAAGCGTCGCGTTGTCCTGGG
TCTGATCGTGGCTGAAGTGGTCAAGCAGTTCGACCTGAAGCCAGACGAAGCCCGCGTTCGCGAAATGATTCAGGAAATGG
CTTCGGCTTACCAAGAGCCTGAGCAGGTCGTGTCCTGGTACTACAAGAACGAGCAGCAACTGAACGAAGTTCGTTCGGTT
GTGCTGGAAGAACAAGTTGTGGATACTGTTCTGCAGAAAGCTAGCGTGACCGACAAATCGGTCTCTTACGAAGAAGCGGT
CAAGCCGGTAGAAGCTCCAAAAGCCGACTGA

Upstream 100 bases:

>100_bases
TTTCAGTAGGGTGACTTCTTGAGTTTGACCTACTAGAATGCTTGCCCTTGATTCTTGGGTCGGAAACGGCCGGCTAACGT
CTGTGCAACGAGGAATATCC

Downstream 100 bases:

>100_bases
TTGTTTTTGCGTTAGAAGCACACACCATAAGCCAGCCTTCGAGCTGGCTTATGCGTATTCAAGACATGACTATTTGGGAG
TGACTGCAGAGCATGTTCCG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MQVSVENTTALERRMSITVPAERIETQVNKRLQQTAQKAKIAGFRPGKVPMSEIKRRFGADARQEAIGDVIQSSFYEAVV
EQKLNPAGSPSIEPKSLEAGKDLEYVAVFEVFPEFTVAGFEGITVERLSADVADADLDKMLDILRKQNTRFEVADRAAQN
EDQLNIDFVGKVDGEVFAGGSAKGTQLVLGSGRMIPGFEEGLVGAKAGEERVLNLTFPEDYQNLDLAGKTAEFTVTVNTV
SEPKLPELNEEFFAQFGIKESGIDGFRTEVRKNMERELRQAIKSKVKNQVMDGLLATNPIEVPKALLSNEVDRLRVQAVQ
QFGGNIKPDQLPAELFEEQAKRRVVLGLIVAEVVKQFDLKPDEARVREMIQEMASAYQEPEQVVSWYYKNEQQLNEVRSV
VLEEQVVDTVLQKASVTDKSVSYEEAVKPVEAPKAD

Sequences:

>Translated_436_residues
MQVSVENTTALERRMSITVPAERIETQVNKRLQQTAQKAKIAGFRPGKVPMSEIKRRFGADARQEAIGDVIQSSFYEAVV
EQKLNPAGSPSIEPKSLEAGKDLEYVAVFEVFPEFTVAGFEGITVERLSADVADADLDKMLDILRKQNTRFEVADRAAQN
EDQLNIDFVGKVDGEVFAGGSAKGTQLVLGSGRMIPGFEEGLVGAKAGEERVLNLTFPEDYQNLDLAGKTAEFTVTVNTV
SEPKLPELNEEFFAQFGIKESGIDGFRTEVRKNMERELRQAIKSKVKNQVMDGLLATNPIEVPKALLSNEVDRLRVQAVQ
QFGGNIKPDQLPAELFEEQAKRRVVLGLIVAEVVKQFDLKPDEARVREMIQEMASAYQEPEQVVSWYYKNEQQLNEVRSV
VLEEQVVDTVLQKASVTDKSVSYEEAVKPVEAPKAD
>Mature_436_residues
MQVSVENTTALERRMSITVPAERIETQVNKRLQQTAQKAKIAGFRPGKVPMSEIKRRFGADARQEAIGDVIQSSFYEAVV
EQKLNPAGSPSIEPKSLEAGKDLEYVAVFEVFPEFTVAGFEGITVERLSADVADADLDKMLDILRKQNTRFEVADRAAQN
EDQLNIDFVGKVDGEVFAGGSAKGTQLVLGSGRMIPGFEEGLVGAKAGEERVLNLTFPEDYQNLDLAGKTAEFTVTVNTV
SEPKLPELNEEFFAQFGIKESGIDGFRTEVRKNMERELRQAIKSKVKNQVMDGLLATNPIEVPKALLSNEVDRLRVQAVQ
QFGGNIKPDQLPAELFEEQAKRRVVLGLIVAEVVKQFDLKPDEARVREMIQEMASAYQEPEQVVSWYYKNEQQLNEVRSV
VLEEQVVDTVLQKASVTDKSVSYEEAVKPVEAPKAD

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=426, Percent_Identity=49.5305164319249, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 48485; Mature: 48485

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSVENTTALERRMSITVPAERIETQVNKRLQQTAQKAKIAGFRPGKVPMSEIKRRFGA
CEECCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
DARQEAIGDVIQSSFYEAVVEQKLNPAGSPSIEPKSLEAGKDLEYVAVFEVFPEFTVAGF
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
EGITVERLSADVADADLDKMLDILRKQNTRFEVADRAAQNEDQLNIDFVGKVDGEVFAGG
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEEECCCCEEECC
SAKGTQLVLGSGRMIPGFEEGLVGAKAGEERVLNLTFPEDYQNLDLAGKTAEFTVTVNTV
CCCCCEEEEECCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEC
SEPKLPELNEEFFAQFGIKESGIDGFRTEVRKNMERELRQAIKSKVKNQVMDGLLATNPI
CCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EVPKALLSNEVDRLRVQAVQQFGGNIKPDQLPAELFEEQAKRRVVLGLIVAEVVKQFDLK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDEARVREMIQEMASAYQEPEQVVSWYYKNEQQLNEVRSVVLEEQVVDTVLQKASVTDKS
CCHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VSYEEAVKPVEAPKAD
CCHHHHCCCCCCCCCC
>Mature Secondary Structure
MQVSVENTTALERRMSITVPAERIETQVNKRLQQTAQKAKIAGFRPGKVPMSEIKRRFGA
CEECCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
DARQEAIGDVIQSSFYEAVVEQKLNPAGSPSIEPKSLEAGKDLEYVAVFEVFPEFTVAGF
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
EGITVERLSADVADADLDKMLDILRKQNTRFEVADRAAQNEDQLNIDFVGKVDGEVFAGG
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEEECCCCEEECC
SAKGTQLVLGSGRMIPGFEEGLVGAKAGEERVLNLTFPEDYQNLDLAGKTAEFTVTVNTV
CCCCCEEEEECCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEC
SEPKLPELNEEFFAQFGIKESGIDGFRTEVRKNMERELRQAIKSKVKNQVMDGLLATNPI
CCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EVPKALLSNEVDRLRVQAVQQFGGNIKPDQLPAELFEEQAKRRVVLGLIVAEVVKQFDLK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDEARVREMIQEMASAYQEPEQVVSWYYKNEQQLNEVRSVVLEEQVVDTVLQKASVTDKS
CCHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VSYEEAVKPVEAPKAD
CCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA