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The map label for this gene is rlmI [H]

Identifier: 77459752

GI number: 77459752

Start: 4007960

End: 4008901

Strand: Direct

Name: rlmI [H]

Synonym: Pfl01_3530

Alternate gene names: 77459752

Gene position: 4007960-4008901 (Clockwise)

Preceding gene: 77459749

Following gene: 77459754

Centisome position: 62.25

GC content: 62.74

Gene sequence:

>942_bases
ATGAACCCTCAAGCCCTCGCCGTTCTCCATGCCCACCTGCTCACCGCGCTGACGTCGGCACCGGCCGAAACCCGGCGCCT
GTTCCATGGGCGCGGGCGTTGCTGGCTGGGGCTGGAGCAAGTCACCGTGGACTGGCTGCAAGGCGTCGTACTGGTGTCGC
TGTTCAAGGAGCCCGAGGCTTCGCAGCTGGACGACCTCAAGCGCCTGCTGCTGGAAATCACCGGTTCGGAGCCATGGCAG
CAGTCCGGCGCCCACACTTTGTTGATCCAGCACCGTTACCTGCCGCAAAGCACCGCCGAATGGCTATTGGGTGAAGAGAT
CGACGAAATGACCATCGTCGAGGGCGGGCTGAAATATCGGGTGGATCTGGGCCGCAAGCAGAACACCGGGCTGTTCCTCG
ACATGCGCTACGGCCGCAACTGGGTGCGCGAACAGGCGGCGGGCAAACGGGTGCTGAACCTGTTCGCCTACACCTGCGGC
TTTTCGGTGGCGGCCATCGAGGGCGGCGCCAGCCATGTGGTCAACCTCGACATGTCCCGCGCCGCGCTGAGCCGGGGCCG
CGACAATCACCGGTTGAACGGGCATGACCTGAGCAAGGTGAGTTTCCTTGGCCATGACCTGTTCAAGTCCTGGGGCAAAG
TGATCAACAGCGGCCCGTACGATCTGGTGATCATCGACCCGCCGTCGTTCCAGAAAGGCAGTTTTCTGCTGACCAAGGAT
TATCAGCGGGTGCTGCGTCGTCTGCCGGAATTGCTGACTGCCCAAGGCAGCGTGCTGGCGTGCATGAACGACCCTTCGTT
CGGCTCCGACTTCCTGATCGACGGCGTGACCCAGGAAGCACCGAGCCTGCGCTTCGAGCAGCGGCTGGAAAATCCGCCGG
AGTTTCCGGATATCGACCCGGAAAGCGGTCTGAAGGCGCTACTGTTCAAGCAGATCGGCTGA

Upstream 100 bases:

>100_bases
TTTTTGATACAGGTCTTTTCGATAACACTCGGTTGATGGGCAGTCGCCGGTACGAAATTGCGGTTAAAATGCGCGCCCTT
TTGCTGGCCGATCCCGATTG

Downstream 100 bases:

>100_bases
TTCCGGTCAGCGCGGGCGCAGCACCAACGCAAACAGCTCACCATGCCCGGTGACGTTGAGCTTGTGGTAGAGATTGCGCC
GGTGCACCTTCACGGTCTCC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNPQALAVLHAHLLTALTSAPAETRRLFHGRGRCWLGLEQVTVDWLQGVVLVSLFKEPEASQLDDLKRLLLEITGSEPWQ
QSGAHTLLIQHRYLPQSTAEWLLGEEIDEMTIVEGGLKYRVDLGRKQNTGLFLDMRYGRNWVREQAAGKRVLNLFAYTCG
FSVAAIEGGASHVVNLDMSRAALSRGRDNHRLNGHDLSKVSFLGHDLFKSWGKVINSGPYDLVIIDPPSFQKGSFLLTKD
YQRVLRRLPELLTAQGSVLACMNDPSFGSDFLIDGVTQEAPSLRFEQRLENPPEFPDIDPESGLKALLFKQIG

Sequences:

>Translated_313_residues
MNPQALAVLHAHLLTALTSAPAETRRLFHGRGRCWLGLEQVTVDWLQGVVLVSLFKEPEASQLDDLKRLLLEITGSEPWQ
QSGAHTLLIQHRYLPQSTAEWLLGEEIDEMTIVEGGLKYRVDLGRKQNTGLFLDMRYGRNWVREQAAGKRVLNLFAYTCG
FSVAAIEGGASHVVNLDMSRAALSRGRDNHRLNGHDLSKVSFLGHDLFKSWGKVINSGPYDLVIIDPPSFQKGSFLLTKD
YQRVLRRLPELLTAQGSVLACMNDPSFGSDFLIDGVTQEAPSLRFEQRLENPPEFPDIDPESGLKALLFKQIG
>Mature_313_residues
MNPQALAVLHAHLLTALTSAPAETRRLFHGRGRCWLGLEQVTVDWLQGVVLVSLFKEPEASQLDDLKRLLLEITGSEPWQ
QSGAHTLLIQHRYLPQSTAEWLLGEEIDEMTIVEGGLKYRVDLGRKQNTGLFLDMRYGRNWVREQAAGKRVLNLFAYTCG
FSVAAIEGGASHVVNLDMSRAALSRGRDNHRLNGHDLSKVSFLGHDLFKSWGKVINSGPYDLVIIDPPSFQKGSFLLTKD
YQRVLRRLPELLTAQGSVLACMNDPSFGSDFLIDGVTQEAPSLRFEQRLENPPEFPDIDPESGLKALLFKQIG

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=122, Percent_Identity=42.6229508196721, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787181, Length=181, Percent_Identity=32.5966850828729, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 34988; Mature: 34988

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPQALAVLHAHLLTALTSAPAETRRLFHGRGRCWLGLEQVTVDWLQGVVLVSLFKEPEA
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHCCCCH
SQLDDLKRLLLEITGSEPWQQSGAHTLLIQHRYLPQSTAEWLLGEEIDEMTIVEGGLKYR
HHHHHHHHHHHHHCCCCCHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEECCCEEEE
VDLGRKQNTGLFLDMRYGRNWVREQAAGKRVLNLFAYTCGFSVAAIEGGASHVVNLDMSR
EECCCCCCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCHHH
AALSRGRDNHRLNGHDLSKVSFLGHDLFKSWGKVINSGPYDLVIIDPPSFQKGSFLLTKD
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEHH
YQRVLRRLPELLTAQGSVLACMNDPSFGSDFLIDGVTQEAPSLRFEQRLENPPEFPDIDP
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEECCCCCCCCCCHHHHHCCCCCCCCCCCH
ESGLKALLFKQIG
HHHHHHHHHHHCC
>Mature Secondary Structure
MNPQALAVLHAHLLTALTSAPAETRRLFHGRGRCWLGLEQVTVDWLQGVVLVSLFKEPEA
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHCCCCH
SQLDDLKRLLLEITGSEPWQQSGAHTLLIQHRYLPQSTAEWLLGEEIDEMTIVEGGLKYR
HHHHHHHHHHHHHCCCCCHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEECCCEEEE
VDLGRKQNTGLFLDMRYGRNWVREQAAGKRVLNLFAYTCGFSVAAIEGGASHVVNLDMSR
EECCCCCCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCHHH
AALSRGRDNHRLNGHDLSKVSFLGHDLFKSWGKVINSGPYDLVIIDPPSFQKGSFLLTKD
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEHH
YQRVLRRLPELLTAQGSVLACMNDPSFGSDFLIDGVTQEAPSLRFEQRLENPPEFPDIDP
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEECCCCCCCCCCHHHHHCCCCCCCCCCCH
ESGLKALLFKQIG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA