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The map label for this gene is potF [H]

Identifier: 77459754

GI number: 77459754

Start: 4009794

End: 4010897

Strand: Direct

Name: potF [H]

Synonym: Pfl01_3532

Alternate gene names: 77459754

Gene position: 4009794-4010897 (Clockwise)

Preceding gene: 77459752

Following gene: 77459755

Centisome position: 62.28

GC content: 62.86

Gene sequence:

>1104_bases
ATGCGTGGTCATCGTGGGTATATCAACCTGGGTCTGATGGGCGTTTTGTCCGCTGCGCTGAGTGCGCAGGCGGCCGATGC
GCCGAGCGTGCATGTCTATAACTGGTACGACTACATCGGCCTTAACACCCTGCATGACTTCAAGCGTGACAGCGGTATCG
AACCGGTCTACGACACCTTCGACAGCGCCGAAGTGCTGGAAGGCAAACTGATGACCAGCCGCAGCGGCTACGACGTGGTG
GTGGCGAGCAACTTCAGCCTGCCGACCCTGATCAAGGCCGGCGCCCTCGCCCCGCTGCCCCGCGACCAACTGCCGGGCTG
GAAGAACCTCGACAACGATCTGCTGAGCAAGCTGGCCAACAACGACCCCGGCAACCAATACGCCGTGCCTTACCTGTGGG
GCACCAACGGCATCGGCTACAACGTCGACAAGGTGCGCGCCGTGCTGGGCGAAAAGGCCCCGGTGGATTCCTGGGATCTG
GTGTTCAAGGAAGAGAACCTGGCCAAACTCGGCGAATGCGGCGTGGCGATGCTCGATTCGCCGTCGGAAATGCTCCCGGT
CGCGCTGCATTATCTCGGCCTGCCGCCCAACAGCACCAACGCCGAGGACTATCAGAAAGCCGAAGCACTGCTGCTGAAAC
TGCGCCCGCACATTGCCTACTTCAATTCCTCGAAATTCATCAGCGATCTGTCCAACGGCAACATTTGCGTGGCGGTCGGC
TGGTCCGGCGCGATGCTCGAAGCCAAGTCCACCGCCGAGCAGGCCGGCAACGGCGTGAAGATCCGGTACAGCCTGCCCAA
GGAAGGCGCGCCAGTGTGGTTCGACACGCTGGTCTTGCTCAAGGACGCACCGCATCCGGCCCAAGGCCTGGCGTTCATCG
ACTACCTGCTGCGGCCCGATGTGATTGCGCCGGTCAGCGATCACTTGTCCTACCCCAACGGCAATCGAGCCGCGACGGCG
CTGGTCGCCCAGACCACCCGCGACAACCCGGCGGTGTACCCGTCAGCCACGGCGATGGCCACGTTGTACACCCTCGAGCC
CTTGCCCAAAGCCACCGAACGGGTGCGCACGCGGGTGTGGAGCAAGGTCAAGAACGGCCAGTAA

Upstream 100 bases:

>100_bases
TCGAGTAACCCATGATGGGAATGGGTGCGGCGCGGCCCGCCCGATAGGCTGACCACTCCTGTTCATCACCGGCCTGCCAA
CAGGCCAGGAGTGCCGCATC

Downstream 100 bases:

>100_bases
CACCCCTTCAAATCACTGCCTTCAACAGAGAGAACATCAATGAAACCACGTGCCCGCGATCTCCACATCCGCATCGGTCA
ATTGCAGCCTGGCCCGCTCA

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MRGHRGYINLGLMGVLSAALSAQAADAPSVHVYNWYDYIGLNTLHDFKRDSGIEPVYDTFDSAEVLEGKLMTSRSGYDVV
VASNFSLPTLIKAGALAPLPRDQLPGWKNLDNDLLSKLANNDPGNQYAVPYLWGTNGIGYNVDKVRAVLGEKAPVDSWDL
VFKEENLAKLGECGVAMLDSPSEMLPVALHYLGLPPNSTNAEDYQKAEALLLKLRPHIAYFNSSKFISDLSNGNICVAVG
WSGAMLEAKSTAEQAGNGVKIRYSLPKEGAPVWFDTLVLLKDAPHPAQGLAFIDYLLRPDVIAPVSDHLSYPNGNRAATA
LVAQTTRDNPAVYPSATAMATLYTLEPLPKATERVRTRVWSKVKNGQ

Sequences:

>Translated_367_residues
MRGHRGYINLGLMGVLSAALSAQAADAPSVHVYNWYDYIGLNTLHDFKRDSGIEPVYDTFDSAEVLEGKLMTSRSGYDVV
VASNFSLPTLIKAGALAPLPRDQLPGWKNLDNDLLSKLANNDPGNQYAVPYLWGTNGIGYNVDKVRAVLGEKAPVDSWDL
VFKEENLAKLGECGVAMLDSPSEMLPVALHYLGLPPNSTNAEDYQKAEALLLKLRPHIAYFNSSKFISDLSNGNICVAVG
WSGAMLEAKSTAEQAGNGVKIRYSLPKEGAPVWFDTLVLLKDAPHPAQGLAFIDYLLRPDVIAPVSDHLSYPNGNRAATA
LVAQTTRDNPAVYPSATAMATLYTLEPLPKATERVRTRVWSKVKNGQ
>Mature_367_residues
MRGHRGYINLGLMGVLSAALSAQAADAPSVHVYNWYDYIGLNTLHDFKRDSGIEPVYDTFDSAEVLEGKLMTSRSGYDVV
VASNFSLPTLIKAGALAPLPRDQLPGWKNLDNDLLSKLANNDPGNQYAVPYLWGTNGIGYNVDKVRAVLGEKAPVDSWDL
VFKEENLAKLGECGVAMLDSPSEMLPVALHYLGLPPNSTNAEDYQKAEALLLKLRPHIAYFNSSKFISDLSNGNICVAVG
WSGAMLEAKSTAEQAGNGVKIRYSLPKEGAPVWFDTLVLLKDAPHPAQGLAFIDYLLRPDVIAPVSDHLSYPNGNRAATA
LVAQTTRDNPAVYPSATAMATLYTLEPLPKATERVRTRVWSKVKNGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=362, Percent_Identity=51.6574585635359, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1787367, Length=320, Percent_Identity=31.875, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1787711, Length=309, Percent_Identity=26.8608414239482, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39889; Mature: 39889

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGHRGYINLGLMGVLSAALSAQAADAPSVHVYNWYDYIGLNTLHDFKRDSGIEPVYDTF
CCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEEECHHHCCCCHHHHHHHCCCCCHHHHCC
DSAEVLEGKLMTSRSGYDVVVASNFSLPTLIKAGALAPLPRDQLPGWKNLDNDLLSKLAN
CCHHHHHCHHEECCCCCEEEEECCCCCHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHC
NDPGNQYAVPYLWGTNGIGYNVDKVRAVLGEKAPVDSWDLVFKEENLAKLGECGVAMLDS
CCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHEEEECCCHHHHHHCCEEEECC
PSEMLPVALHYLGLPPNSTNAEDYQKAEALLLKLRPHIAYFNSSKFISDLSNGNICVAVG
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCEEEEEC
WSGAMLEAKSTAEQAGNGVKIRYSLPKEGAPVWFDTLVLLKDAPHPAQGLAFIDYLLRPD
CCCCEEECHHHHHHCCCCEEEEEECCCCCCCHHHHHEEEEECCCCCHHHHHHHHHHHCCC
VIAPVSDHLSYPNGNRAATALVAQTTRDNPAVYPSATAMATLYTLEPLPKATERVRTRVW
CCCCHHHHCCCCCCCHHEEEEEEECCCCCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHH
SKVKNGQ
HHHCCCC
>Mature Secondary Structure
MRGHRGYINLGLMGVLSAALSAQAADAPSVHVYNWYDYIGLNTLHDFKRDSGIEPVYDTF
CCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEEECHHHCCCCHHHHHHHCCCCCHHHHCC
DSAEVLEGKLMTSRSGYDVVVASNFSLPTLIKAGALAPLPRDQLPGWKNLDNDLLSKLAN
CCHHHHHCHHEECCCCCEEEEECCCCCHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHC
NDPGNQYAVPYLWGTNGIGYNVDKVRAVLGEKAPVDSWDLVFKEENLAKLGECGVAMLDS
CCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHEEEECCCHHHHHHCCEEEECC
PSEMLPVALHYLGLPPNSTNAEDYQKAEALLLKLRPHIAYFNSSKFISDLSNGNICVAVG
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCEEEEEC
WSGAMLEAKSTAEQAGNGVKIRYSLPKEGAPVWFDTLVLLKDAPHPAQGLAFIDYLLRPD
CCCCEEECHHHHHHCCCCEEEEEECCCCCCCHHHHHEEEEECCCCCHHHHHHHHHHHCCC
VIAPVSDHLSYPNGNRAATALVAQTTRDNPAVYPSATAMATLYTLEPLPKATERVRTRVW
CCCCHHHHCCCCCCCHHEEEEEEECCCCCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHH
SKVKNGQ
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]