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The map label for this gene is irlS [H]

Identifier: 77459742

GI number: 77459742

Start: 3999367

End: 4000761

Strand: Direct

Name: irlS [H]

Synonym: Pfl01_3520

Alternate gene names: 77459742

Gene position: 3999367-4000761 (Clockwise)

Preceding gene: 77459741

Following gene: 77459746

Centisome position: 62.12

GC content: 62.15

Gene sequence:

>1395_bases
ATGTCATTGCCGAACCCGTCTGACGGTTGGCGCTCTTCCAGCAGCCGCTTGCTGGCGCTGTACAGTTCGTTGTTCGTGGC
CTGGAGCGCGATCCTCATGGGGGTCATGTACTTCGAGGTTTCCGGTTATCTCGACAACCTGGCCAAGCATTCGCTGATGC
AACGTCAGCACCTGTTCAAGCATTTTCGCGGCGATCAACTGGAAGAGGCCCTCGCCGCCAGCATGACCTTCGACATTCGC
GGCATCGACGCCTACGGCCTGTTTGACGCCCAGCATCGCTACCTCGGCGGCGCGTTGCAGAAGATCCCCGAGGGCCTGCC
GCTGGACGGCAAGATCCACATGCTCAGCGAATGCGCCGACTCCGACGACCCGACCCTGCCCGCCGACAGCTGCGACGCTG
TCGCCACCCCGACCCGAGACGGCCGCTGGCTGGTGTTGTTGCGTGACAACGGTTCGCTGTTCGCGGTGACCAGGATCATT
CTGCATGCGTTGCTCTGGGGCGTATCGCTGACGATTGTGCCGGGCATCATCGGCTGGCATTTGCTGCGTCGCCGGCCGTT
GCGGCGGATCCGGGCGATCCAGGCCAGCGCCCAGTCGATTGTCGCCGGGGACCTGACCCACCGTTTGCCGCTGTCCAATC
GCCGCGATGAGCTGGACATGCTCGCCGCCATCGTCAACGCCATGCTCGATCGCATCGAGCGCTTGATGAATGAAGTCAAA
GGCGTGTGCGACAACATTGCCCATGACCTGCGCACCCCGCTGACCCGATTGCGCGCGCAGTTGTATCGCATGCAGCAGCA
GGCTGGCGAAGGTTCGCCGGAAGCGGCGCAACTGGATCTGGTGCTGGCCGAGGCCGATACGTTGATGGCGCGGTTTCGGG
GTTTATTGAGGATTTCCGAACTGGAGGATCGTCAGCGCCGCTCGGGGTTTGTCGAGCTCGATCCGGTGTTGCTGCTGCAG
GAATTGCACGAGTTCTATCTGCCGCTGGCCGAAGACGACGAGTTGCGCTTCGAACTGAACATGCCGGAAACCCTGCCCAT
GCTGAACGGTGATCGAGCGTTGCTGTTTGAAGCGCTGGCGAACCTGTTGAGTAACTCGATCAAGTTCACCCCGGCCGGTG
GCACGGTGGTGTTGCGCGGGGTCAATGATGCGGGGCATACGCGGATCGAGGTGCACGACTCCGGGCCCGGCATTCCAGAA
TCCGAACGGGAGGCGGTGTTCCAGCGCTTCTATCGCGCCGAGGGCGGGCAACCGCAGAATGGTTTTGGCCTGGGACTGTC
GATCGTCGCGGCGATCGTCAATCTGCACGGTTTCAGTCTTGAGGTGGGACGCAGCGATCTGGGCGGGGCGAAGCTGGTGC
TGGATTGTCGGCAGAGTCTGATTTCACAGTCTTGA

Upstream 100 bases:

>100_bases
CCCGGCACCAACCTGATCGACGTGCACATCGGCCGTCTGCGCAAGAAGATCGACCCGCCGGGCAATGTCCCGCTGATCCG
TACGGTGCGAGGCTCGGGTT

Downstream 100 bases:

>100_bases
AGAGATCGTTCCCACGCCGCGTGGGAACGATCAGCGATCAACTTAAGCGGGGTAGTTGGCGCGCAGTGCCTCGAGCCCAC
CCTGATAGATCCCGCTGAAC

Product: periplasmic sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSLPNPSDGWRSSSSRLLALYSSLFVAWSAILMGVMYFEVSGYLDNLAKHSLMQRQHLFKHFRGDQLEEALAASMTFDIR
GIDAYGLFDAQHRYLGGALQKIPEGLPLDGKIHMLSECADSDDPTLPADSCDAVATPTRDGRWLVLLRDNGSLFAVTRII
LHALLWGVSLTIVPGIIGWHLLRRRPLRRIRAIQASAQSIVAGDLTHRLPLSNRRDELDMLAAIVNAMLDRIERLMNEVK
GVCDNIAHDLRTPLTRLRAQLYRMQQQAGEGSPEAAQLDLVLAEADTLMARFRGLLRISELEDRQRRSGFVELDPVLLLQ
ELHEFYLPLAEDDELRFELNMPETLPMLNGDRALLFEALANLLSNSIKFTPAGGTVVLRGVNDAGHTRIEVHDSGPGIPE
SEREAVFQRFYRAEGGQPQNGFGLGLSIVAAIVNLHGFSLEVGRSDLGGAKLVLDCRQSLISQS

Sequences:

>Translated_464_residues
MSLPNPSDGWRSSSSRLLALYSSLFVAWSAILMGVMYFEVSGYLDNLAKHSLMQRQHLFKHFRGDQLEEALAASMTFDIR
GIDAYGLFDAQHRYLGGALQKIPEGLPLDGKIHMLSECADSDDPTLPADSCDAVATPTRDGRWLVLLRDNGSLFAVTRII
LHALLWGVSLTIVPGIIGWHLLRRRPLRRIRAIQASAQSIVAGDLTHRLPLSNRRDELDMLAAIVNAMLDRIERLMNEVK
GVCDNIAHDLRTPLTRLRAQLYRMQQQAGEGSPEAAQLDLVLAEADTLMARFRGLLRISELEDRQRRSGFVELDPVLLLQ
ELHEFYLPLAEDDELRFELNMPETLPMLNGDRALLFEALANLLSNSIKFTPAGGTVVLRGVNDAGHTRIEVHDSGPGIPE
SEREAVFQRFYRAEGGQPQNGFGLGLSIVAAIVNLHGFSLEVGRSDLGGAKLVLDCRQSLISQS
>Mature_463_residues
SLPNPSDGWRSSSSRLLALYSSLFVAWSAILMGVMYFEVSGYLDNLAKHSLMQRQHLFKHFRGDQLEEALAASMTFDIRG
IDAYGLFDAQHRYLGGALQKIPEGLPLDGKIHMLSECADSDDPTLPADSCDAVATPTRDGRWLVLLRDNGSLFAVTRIIL
HALLWGVSLTIVPGIIGWHLLRRRPLRRIRAIQASAQSIVAGDLTHRLPLSNRRDELDMLAAIVNAMLDRIERLMNEVKG
VCDNIAHDLRTPLTRLRAQLYRMQQQAGEGSPEAAQLDLVLAEADTLMARFRGLLRISELEDRQRRSGFVELDPVLLLQE
LHEFYLPLAEDDELRFELNMPETLPMLNGDRALLFEALANLLSNSIKFTPAGGTVVLRGVNDAGHTRIEVHDSGPGIPES
EREAVFQRFYRAEGGQPQNGFGLGLSIVAAIVNLHGFSLEVGRSDLGGAKLVLDCRQSLISQS

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=268, Percent_Identity=26.865671641791, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786783, Length=273, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790861, Length=393, Percent_Identity=27.4809160305344, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790346, Length=268, Percent_Identity=28.3582089552239, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786912, Length=207, Percent_Identity=29.951690821256, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787894, Length=298, Percent_Identity=27.1812080536913, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788279, Length=269, Percent_Identity=26.0223048327138, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI48994928, Length=231, Percent_Identity=27.7056277056277, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI145693157, Length=195, Percent_Identity=30.2564102564103, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=26.1261261261261, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790551, Length=309, Percent_Identity=28.8025889967638, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789808, Length=224, Percent_Identity=26.3392857142857, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=25.9911894273128, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788713, Length=205, Percent_Identity=25.8536585365854, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789403, Length=238, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789149, Length=203, Percent_Identity=29.5566502463054, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788549, Length=210, Percent_Identity=26.1904761904762, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51459; Mature: 51328

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPNPSDGWRSSSSRLLALYSSLFVAWSAILMGVMYFEVSGYLDNLAKHSLMQRQHLFK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFRGDQLEEALAASMTFDIRGIDAYGLFDAQHRYLGGALQKIPEGLPLDGKIHMLSECAD
HHCCCHHHHHHHHHHEEEEECCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
SDDPTLPADSCDAVATPTRDGRWLVLLRDNGSLFAVTRIILHALLWGVSLTIVPGIIGWH
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLRRRPLRRIRAIQASAQSIVAGDLTHRLPLSNRRDELDMLAAIVNAMLDRIERLMNEVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GVCDNIAHDLRTPLTRLRAQLYRMQQQAGEGSPEAAQLDLVLAEADTLMARFRGLLRISE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHH
LEDRQRRSGFVELDPVLLLQELHEFYLPLAEDDELRFELNMPETLPMLNGDRALLFEALA
HHHHHHHCCCEEECHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
NLLSNSIKFTPAGGTVVLRGVNDAGHTRIEVHDSGPGIPESEREAVFQRFYRAEGGQPQN
HHHHCCCEEECCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCC
GFGLGLSIVAAIVNLHGFSLEVGRSDLGGAKLVLDCRQSLISQS
CCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLPNPSDGWRSSSSRLLALYSSLFVAWSAILMGVMYFEVSGYLDNLAKHSLMQRQHLFK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFRGDQLEEALAASMTFDIRGIDAYGLFDAQHRYLGGALQKIPEGLPLDGKIHMLSECAD
HHCCCHHHHHHHHHHEEEEECCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
SDDPTLPADSCDAVATPTRDGRWLVLLRDNGSLFAVTRIILHALLWGVSLTIVPGIIGWH
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLRRRPLRRIRAIQASAQSIVAGDLTHRLPLSNRRDELDMLAAIVNAMLDRIERLMNEVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GVCDNIAHDLRTPLTRLRAQLYRMQQQAGEGSPEAAQLDLVLAEADTLMARFRGLLRISE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHH
LEDRQRRSGFVELDPVLLLQELHEFYLPLAEDDELRFELNMPETLPMLNGDRALLFEALA
HHHHHHHCCCEEECHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
NLLSNSIKFTPAGGTVVLRGVNDAGHTRIEVHDSGPGIPESEREAVFQRFYRAEGGQPQN
HHHHCCCEEECCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCC
GFGLGLSIVAAIVNLHGFSLEVGRSDLGGAKLVLDCRQSLISQS
CCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]