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The map label for this gene is alsR [H]

Identifier: 77459746

GI number: 77459746

Start: 4003169

End: 4004065

Strand: Direct

Name: alsR [H]

Synonym: Pfl01_3524

Alternate gene names: 77459746

Gene position: 4003169-4004065 (Clockwise)

Preceding gene: 77459742

Following gene: 77459747

Centisome position: 62.18

GC content: 64.77

Gene sequence:

>897_bases
ATGATCGACATCCGCCAATTGCGCTACTTCGTCGCCGTCGCCGAGGAAGAACACGTCGGCCGCGCCGCCGAGCGCCTGCA
CATTTCCCAGTCGCCCCTGAGCCGGCAGATCGCCCAGCTCGAAGAGCGCCTGGGCCTGACTCTGTTCGAACGCAGCCAGC
AACGCATCCGCCTGACCCGCGACGGCCAGACCTTTCTCGCCGAAACCCGCGCCCTGCTGACCCACGCCAACCGCTTGGAA
TCCCTCGGCAAACGCCTGGGCCGTGGCGAAGAAGGTGGCCTGTGCATCGGCTACATCGAGAACGCGATGCACGCCGGGGT
CTTGCCCAACGCCTTGCGCGTGCTGCGGGTGGATCGGCCGAACGTGCATGTCGCGCTGTACAACCTGAGTTCCGCCGAAC
AACTGGAAGGCCTGCGTCAGCGTAGCCTCGATATCGCGCTGGTCAGTGAACCGCCGACGATCGACGACCCTGACCTGCTG
GGCTTTCAGGTGCTGGACGACCCGATGCTGCTGGCGCTGCCCGAACATCACCCGCTGAATCAATTGCCATTGCTGACCCC
GGCGGATCTGGCCGATCAGGAATGGATTGGCGTGCAACCGCGCCAAGGGGCCGACGATGAGTTCGTCAGCGCCTGCATCC
GCGCAGGTTTCACCCCGGATGTGCGGATGCAGGCCACGGAACCGTTTACCGCGTTGGGGCTGGTGGCCTCGGGGCTGGGG
ATCGCGATGATCCAGAAAGGCCTGAGCCATAACGCACCGCCGGGCGTGGTGCTGCGGGAAGTGCCGTGGCTGGCGTTTAC
CACGCCGTTGTGGGCGGCTTGGCACCGAGTCAATTTACGGCCGTTGGTGGAGACGTTTCGTAAAGTGTTGACCGGGTCCG
ATATCTCCGCCGCCTGA

Upstream 100 bases:

>100_bases
GTAATTCGTGGTCAGTGTTGACCGTTGAGCAGATCCTAGATTGCGCCACTCAGACCGTCCAAGACATAATCCGCAGCACT
TGAGTCCCTGAAGGTCTGAC

Downstream 100 bases:

>100_bases
AAATCCGCAGGTCCTCACCCCAGCCCTCTCGAAACGTCGGACCGCCCGTAGGGAGAGGGAACCTATCGAGGGATATTGAC
GTGATAAATCGACCTGAAAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MIDIRQLRYFVAVAEEEHVGRAAERLHISQSPLSRQIAQLEERLGLTLFERSQQRIRLTRDGQTFLAETRALLTHANRLE
SLGKRLGRGEEGGLCIGYIENAMHAGVLPNALRVLRVDRPNVHVALYNLSSAEQLEGLRQRSLDIALVSEPPTIDDPDLL
GFQVLDDPMLLALPEHHPLNQLPLLTPADLADQEWIGVQPRQGADDEFVSACIRAGFTPDVRMQATEPFTALGLVASGLG
IAMIQKGLSHNAPPGVVLREVPWLAFTTPLWAAWHRVNLRPLVETFRKVLTGSDISAA

Sequences:

>Translated_298_residues
MIDIRQLRYFVAVAEEEHVGRAAERLHISQSPLSRQIAQLEERLGLTLFERSQQRIRLTRDGQTFLAETRALLTHANRLE
SLGKRLGRGEEGGLCIGYIENAMHAGVLPNALRVLRVDRPNVHVALYNLSSAEQLEGLRQRSLDIALVSEPPTIDDPDLL
GFQVLDDPMLLALPEHHPLNQLPLLTPADLADQEWIGVQPRQGADDEFVSACIRAGFTPDVRMQATEPFTALGLVASGLG
IAMIQKGLSHNAPPGVVLREVPWLAFTTPLWAAWHRVNLRPLVETFRKVLTGSDISAA
>Mature_298_residues
MIDIRQLRYFVAVAEEEHVGRAAERLHISQSPLSRQIAQLEERLGLTLFERSQQRIRLTRDGQTFLAETRALLTHANRLE
SLGKRLGRGEEGGLCIGYIENAMHAGVLPNALRVLRVDRPNVHVALYNLSSAEQLEGLRQRSLDIALVSEPPTIDDPDLL
GFQVLDDPMLLALPEHHPLNQLPLLTPADLADQEWIGVQPRQGADDEFVSACIRAGFTPDVRMQATEPFTALGLVASGLG
IAMIQKGLSHNAPPGVVLREVPWLAFTTPLWAAWHRVNLRPLVETFRKVLTGSDISAA

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=281, Percent_Identity=33.4519572953737, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI2367136, Length=263, Percent_Identity=36.5019011406844, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788887, Length=290, Percent_Identity=28.9655172413793, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=30.8943089430894, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI157672245, Length=235, Percent_Identity=28.936170212766, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI145693105, Length=261, Percent_Identity=24.904214559387, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790208, Length=304, Percent_Identity=27.6315789473684, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787806, Length=298, Percent_Identity=25.503355704698, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788748, Length=252, Percent_Identity=24.6031746031746, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788296, Length=251, Percent_Identity=27.4900398406374, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87081904, Length=263, Percent_Identity=29.6577946768061, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787530, Length=245, Percent_Identity=24.8979591836735, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788297, Length=149, Percent_Identity=28.1879194630872, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789204, Length=265, Percent_Identity=27.5471698113208, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790262, Length=249, Percent_Identity=25.7028112449799, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32989; Mature: 32989

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDIRQLRYFVAVAEEEHVGRAAERLHISQSPLSRQIAQLEERLGLTLFERSQQRIRLTR
CCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHCCHHHEEEEC
DGQTFLAETRALLTHANRLESLGKRLGRGEEGGLCIGYIENAMHAGVLPNALRVLRVDRP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHCCCCHHHHHEEEECCC
NVHVALYNLSSAEQLEGLRQRSLDIALVSEPPTIDDPDLLGFQVLDDPMLLALPEHHPLN
CEEEEEEECCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCCCC
QLPLLTPADLADQEWIGVQPRQGADDEFVSACIRAGFTPDVRMQATEPFTALGLVASGLG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHCHH
IAMIQKGLSHNAPPGVVLREVPWLAFTTPLWAAWHRVNLRPLVETFRKVLTGSDISAA
HHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIDIRQLRYFVAVAEEEHVGRAAERLHISQSPLSRQIAQLEERLGLTLFERSQQRIRLTR
CCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHCCHHHEEEEC
DGQTFLAETRALLTHANRLESLGKRLGRGEEGGLCIGYIENAMHAGVLPNALRVLRVDRP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHCCCCHHHHHEEEECCC
NVHVALYNLSSAEQLEGLRQRSLDIALVSEPPTIDDPDLLGFQVLDDPMLLALPEHHPLN
CEEEEEEECCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCCCC
QLPLLTPADLADQEWIGVQPRQGADDEFVSACIRAGFTPDVRMQATEPFTALGLVASGLG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHCHH
IAMIQKGLSHNAPPGVVLREVPWLAFTTPLWAAWHRVNLRPLVETFRKVLTGSDISAA
HHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]