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The map label for this gene is citH [H]

Identifier: 77459732

GI number: 77459732

Start: 3988699

End: 3989991

Strand: Direct

Name: citH [H]

Synonym: Pfl01_3510

Alternate gene names: 77459732

Gene position: 3988699-3989991 (Clockwise)

Preceding gene: 77459730

Following gene: 77459733

Centisome position: 61.95

GC content: 60.94

Gene sequence:

>1293_bases
ATGACAGCCTCAATCCCACACGGCGGCTCGCGGGCCGGCGCCATTTTCCGGGTGACCTCGGGTAACTTCCTCGAACAGTT
CGACTTCTTTCTGTTCGGCTTCTACGCCACGCAGATCGCGGCGGTGTTCTTTCCGGCGAGCAGTGAGTTCGCGTCCCTGA
TGATGACTTTCGCAGTGTTCGGCGCAGGCTTTTTGATGCGTCCGCTGGGGGCTATCGTGCTCGGCGCGTACATTGATGAC
GTCGGTCGGCGCAAAGGGTTGATTGTCACCCTGTCGATCATGGCCAGCGGCACGATCTTGATTGTGCTGGTGCCCGGATA
CGAAACCATTGGCCTGTTCGCCCCGGCGCTGGTGTTGATCGGAAGGCTGTTGCAAGGTTTCTCCGCGGGTGCGGAATTGG
GCGGGGTTTCGGTGTATCTCTCGGAGATCGCTACACCCGGTCGCAAAGGCTTTTTCACCGCCTGGCAGTCGGCCAGTCAG
CAGGTGGCCATCGTCGTCGCCGCCGCGCTCGGTTACGGTTTGAACCAGTGGATGGCGCCGGAAGCGGTCGCCGATTGGGG
CTGGCGGATTCCGTTTTTCGTCGGCTGCATGATCGTTCCTTTCATCTTCTTTCTGCGGCGCAATCTGGCGGAAACCGAAG
AGTTCGCCGCTCGCAAACATCGTCCAAGCATGAAGGAAGTGTTCCGCACCCTCGGTCAGAACTTGGGCGTGGTGCTGGGC
GGGATGCTGATGGTTGCCCTGACTACCACTGCGTTCTACCTGATCACCGTGTACGCACCGACCTTCGGCAAAACCGTGCT
GCACCTGAGCACATCCGATGCGTTGCTGGTGACGTTGCTGGTGGGCGTTTCCAACTTCTTCTGGCTGCCGATTGGCGGTG
CATTGTCCGATCGCGTCGGCCGCCGTCCAGTGCTGATCGCCATGGCGCTGCTGGCTCTGGCCACCACGTATCCAGCGCTG
TCGTACCTGGTGCAGGCGCCGAGCTTCAGTCACATGCTGCTGTCGCTGTTGTGGCTGTCGTTTATCTACGGCCTGTACAA
CGGCGCGATGATTCCCGCGCTGACCGAGATCATGCCGGTGGAAGTGCGGGTGGCCGGTTTCTCCCTGGCCTATAGCCTCG
CCACTGCCGTGTTCGGCGGATTCACTCCAGCGATGTCGACCTTCCTGATCCAGTACACCGGCGACAAAGCCGCACCAGGT
TACTGGATGAGCATCGGCGCGCTCTGCGCATTGGGCGCGACGCTTTATCTGTATCGCCGGGCCGGCGGTCGTCTGCAACC
TGTTACTGCCTGA

Upstream 100 bases:

>100_bases
CAATTAACAAATCGATCCCTGCGCGCCACTATCCGCCCCAATAAGAACCGTGCGCCGCCTCCCCGTCGCGCACGTCATAA
AAGCGGTGGAGTACTTTTCT

Downstream 100 bases:

>100_bases
GAACATCACCATGAAAAAACTGTTTACCGTTACCGCCCTGCTCGCCGGCCTCGCATTCAACGTTGCGGCCCAGGCCGAAG
AACTGCGGGTCATGACTTCC

Product: citrate-proton symport

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTASIPHGGSRAGAIFRVTSGNFLEQFDFFLFGFYATQIAAVFFPASSEFASLMMTFAVFGAGFLMRPLGAIVLGAYIDD
VGRRKGLIVTLSIMASGTILIVLVPGYETIGLFAPALVLIGRLLQGFSAGAELGGVSVYLSEIATPGRKGFFTAWQSASQ
QVAIVVAAALGYGLNQWMAPEAVADWGWRIPFFVGCMIVPFIFFLRRNLAETEEFAARKHRPSMKEVFRTLGQNLGVVLG
GMLMVALTTTAFYLITVYAPTFGKTVLHLSTSDALLVTLLVGVSNFFWLPIGGALSDRVGRRPVLIAMALLALATTYPAL
SYLVQAPSFSHMLLSLLWLSFIYGLYNGAMIPALTEIMPVEVRVAGFSLAYSLATAVFGGFTPAMSTFLIQYTGDKAAPG
YWMSIGALCALGATLYLYRRAGGRLQPVTA

Sequences:

>Translated_430_residues
MTASIPHGGSRAGAIFRVTSGNFLEQFDFFLFGFYATQIAAVFFPASSEFASLMMTFAVFGAGFLMRPLGAIVLGAYIDD
VGRRKGLIVTLSIMASGTILIVLVPGYETIGLFAPALVLIGRLLQGFSAGAELGGVSVYLSEIATPGRKGFFTAWQSASQ
QVAIVVAAALGYGLNQWMAPEAVADWGWRIPFFVGCMIVPFIFFLRRNLAETEEFAARKHRPSMKEVFRTLGQNLGVVLG
GMLMVALTTTAFYLITVYAPTFGKTVLHLSTSDALLVTLLVGVSNFFWLPIGGALSDRVGRRPVLIAMALLALATTYPAL
SYLVQAPSFSHMLLSLLWLSFIYGLYNGAMIPALTEIMPVEVRVAGFSLAYSLATAVFGGFTPAMSTFLIQYTGDKAAPG
YWMSIGALCALGATLYLYRRAGGRLQPVTA
>Mature_429_residues
TASIPHGGSRAGAIFRVTSGNFLEQFDFFLFGFYATQIAAVFFPASSEFASLMMTFAVFGAGFLMRPLGAIVLGAYIDDV
GRRKGLIVTLSIMASGTILIVLVPGYETIGLFAPALVLIGRLLQGFSAGAELGGVSVYLSEIATPGRKGFFTAWQSASQQ
VAIVVAAALGYGLNQWMAPEAVADWGWRIPFFVGCMIVPFIFFLRRNLAETEEFAARKHRPSMKEVFRTLGQNLGVVLGG
MLMVALTTTAFYLITVYAPTFGKTVLHLSTSDALLVTLLVGVSNFFWLPIGGALSDRVGRRPVLIAMALLALATTYPALS
YLVQAPSFSHMLLSLLWLSFIYGLYNGAMIPALTEIMPVEVRVAGFSLAYSLATAVFGGFTPAMSTFLIQYTGDKAAPGY
WMSIGALCALGATLYLYRRAGGRLQPVTA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=426, Percent_Identity=30.0469483568075, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1788942, Length=426, Percent_Identity=34.5070422535211, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1788292, Length=395, Percent_Identity=29.3670886075949, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789941, Length=395, Percent_Identity=29.1139240506329, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87082231, Length=211, Percent_Identity=28.9099526066351, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46157; Mature: 46026

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASIPHGGSRAGAIFRVTSGNFLEQFDFFLFGFYATQIAAVFFPASSEFASLMMTFAVF
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GAGFLMRPLGAIVLGAYIDDVGRRKGLIVTLSIMASGTILIVLVPGYETIGLFAPALVLI
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHH
GRLLQGFSAGAELGGVSVYLSEIATPGRKGFFTAWQSASQQVAIVVAAALGYGLNQWMAP
HHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCHHHCCCC
EAVADWGWRIPFFVGCMIVPFIFFLRRNLAETEEFAARKHRPSMKEVFRTLGQNLGVVLG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHH
GMLMVALTTTAFYLITVYAPTFGKTVLHLSTSDALLVTLLVGVSNFFWLPIGGALSDRVG
HHHHHHHHHHHHHHHHEECCCCCCEEEEECCCHHHHHHHHHHHHHEEEEECCCHHHHHCC
RRPVLIAMALLALATTYPALSYLVQAPSFSHMLLSLLWLSFIYGLYNGAMIPALTEIMPV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCE
EVRVAGFSLAYSLATAVFGGFTPAMSTFLIQYTGDKAAPGYWMSIGALCALGATLYLYRR
EEEEEHHHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
AGGRLQPVTA
CCCCCCCCCC
>Mature Secondary Structure 
TASIPHGGSRAGAIFRVTSGNFLEQFDFFLFGFYATQIAAVFFPASSEFASLMMTFAVF
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GAGFLMRPLGAIVLGAYIDDVGRRKGLIVTLSIMASGTILIVLVPGYETIGLFAPALVLI
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHH
GRLLQGFSAGAELGGVSVYLSEIATPGRKGFFTAWQSASQQVAIVVAAALGYGLNQWMAP
HHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCHHHCCCC
EAVADWGWRIPFFVGCMIVPFIFFLRRNLAETEEFAARKHRPSMKEVFRTLGQNLGVVLG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHH
GMLMVALTTTAFYLITVYAPTFGKTVLHLSTSDALLVTLLVGVSNFFWLPIGGALSDRVG
HHHHHHHHHHHHHHHHEECCCCCCEEEEECCCHHHHHHHHHHHHHEEEEECCCHHHHHCC
RRPVLIAMALLALATTYPALSYLVQAPSFSHMLLSLLWLSFIYGLYNGAMIPALTEIMPV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCE
EVRVAGFSLAYSLATAVFGGFTPAMSTFLIQYTGDKAAPGYWMSIGALCALGATLYLYRR
EEEEEHHHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
AGGRLQPVTA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]