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The map label for this gene is nitA [H]

Identifier: 77458796

GI number: 77458796

Start: 2947716

End: 2948639

Strand: Direct

Name: nitA [H]

Synonym: Pfl01_2571

Alternate gene names: 77458796

Gene position: 2947716-2948639 (Clockwise)

Preceding gene: 77458794

Following gene: 77458800

Centisome position: 45.78

GC content: 64.5

Gene sequence:

>924_bases
ATGCCCAAATCAATCGTTGCCGCCCTGCAGATCGGCGCCTTGCCCGGTGGCAAGGCTGAAACCCTGGAACAGATCCTGAG
CTGGGAAACCGCGATCATCGAATCCGGCGCCGCGCTGGTGGTAATGCCCGAAGCCTTGCTCGGTGGTTACCCGAAAGGCG
AGGGCTTCGGCACGCAGCTGGGTTATCGCTTGCCGGAAGGCCGCGAGGCCTACGCCCGTTACTTCGCCAACGCTATCGAC
GTGCCGGGGGCGGAAACCGAAGCGCTGGCCGGTCTGTCGGCACGTACCGGGGCCAATCTGGTGATCGGCGTGATCGAACG
GGCCGGCAGCACTTTGCATTGCACCGCGCTGTATTTCGACCCGCAGGCCGGCCTAGTGGCCAAGCACCGCAAGTTGATGC
CGACCGGCACCGAGCGGCTGATCTGGGGCAAGGGCGACGGTTCAACGTTGCCGGTGCTCGACACGCAGGTCGGCAAGCTC
GGCGCAGTGATCTGCTGGGAAAACATGATGCCGCTGCTGCGCACGGCGATGTACGCCAAGGGCATTGAAGTGTGGTGTGC
GCCGACTGTGGATGAGCGCGAGATGTGGCAGGTCAGCATGCGTCACATCGCCCATGAAGGGCGCTGCTTCGTGGTCAGCG
CCTGTCAGGTGCAGGCCTCGCCGAATGATCTGGGCGTGGAAATTGACAACTGGCCGGGAGATCGACCGCTGATCGCCGGC
GGCAGCGTGATCGTCGGGCCGATGGGCGACGTATTGGCCGGGCCGTTGCGCGGCGAGGCCGGCTTGCTCACCGCCGAGAT
CGACACGGAAGAACTGGTGCGCGCCCGCTACGACTACGACGTGGTCGGCCACTATGCACGGCCCGATGTGTTCGAGCTGA
GCGTCGATGAGCGGGCTAAACCCGGTGTACGTTTCACCACTTGA

Upstream 100 bases:

>100_bases
TATTCATTTGGCTCATATCATTCAGCAGTGGATCGGTCTTTATTCATGATCCCGTGGCGTCGGAGAATCAGCAACACCTG
AACTTCACGGAGTGACCGCC

Downstream 100 bases:

>100_bases
CGACGGTGCGGTTTACCAGGTGGCAGCACTGGCCCTGGGCAGACTGAGCTTGCCGGTATCGACAAAGCGCATCGTGCCAA
ACAACCCGCCCGCCAGTTTG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPKSIVAALQIGALPGGKAETLEQILSWETAIIESGAALVVMPEALLGGYPKGEGFGTQLGYRLPEGREAYARYFANAID
VPGAETEALAGLSARTGANLVIGVIERAGSTLHCTALYFDPQAGLVAKHRKLMPTGTERLIWGKGDGSTLPVLDTQVGKL
GAVICWENMMPLLRTAMYAKGIEVWCAPTVDEREMWQVSMRHIAHEGRCFVVSACQVQASPNDLGVEIDNWPGDRPLIAG
GSVIVGPMGDVLAGPLRGEAGLLTAEIDTEELVRARYDYDVVGHYARPDVFELSVDERAKPGVRFTT

Sequences:

>Translated_307_residues
MPKSIVAALQIGALPGGKAETLEQILSWETAIIESGAALVVMPEALLGGYPKGEGFGTQLGYRLPEGREAYARYFANAID
VPGAETEALAGLSARTGANLVIGVIERAGSTLHCTALYFDPQAGLVAKHRKLMPTGTERLIWGKGDGSTLPVLDTQVGKL
GAVICWENMMPLLRTAMYAKGIEVWCAPTVDEREMWQVSMRHIAHEGRCFVVSACQVQASPNDLGVEIDNWPGDRPLIAG
GSVIVGPMGDVLAGPLRGEAGLLTAEIDTEELVRARYDYDVVGHYARPDVFELSVDERAKPGVRFTT
>Mature_306_residues
PKSIVAALQIGALPGGKAETLEQILSWETAIIESGAALVVMPEALLGGYPKGEGFGTQLGYRLPEGREAYARYFANAIDV
PGAETEALAGLSARTGANLVIGVIERAGSTLHCTALYFDPQAGLVAKHRKLMPTGTERLIWGKGDGSTLPVLDTQVGKLG
AVICWENMMPLLRTAMYAKGIEVWCAPTVDEREMWQVSMRHIAHEGRCFVVSACQVQASPNDLGVEIDNWPGDRPLIAGG
SVIVGPMGDVLAGPLRGEAGLLTAEIDTEELVRARYDYDVVGHYARPDVFELSVDERAKPGVRFTT

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=305, Percent_Identity=46.8852459016393, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6322027, Length=197, Percent_Identity=63.9593908629442, Blast_Score=258, Evalue=8e-70,
Organism=Saccharomyces cerevisiae, GI6322026, Length=119, Percent_Identity=46.218487394958, Blast_Score=103, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 32889; Mature: 32758

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSIVAALQIGALPGGKAETLEQILSWETAIIESGAALVVMPEALLGGYPKGEGFGTQL
CCCHHEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCCCCCC
GYRLPEGREAYARYFANAIDVPGAETEALAGLSARTGANLVIGVIERAGSTLHCTALYFD
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEEEEHHHCCCEEEEEEEEEC
PQAGLVAKHRKLMPTGTERLIWGKGDGSTLPVLDTQVGKLGAVICWENMMPLLRTAMYAK
CCCCHHHHHHCCCCCCCCEEEEECCCCCCCCEECCCCCHHEEEEEHHHHHHHHHHHHHHC
GIEVWCAPTVDEREMWQVSMRHIAHEGRCFVVSACQVQASPNDLGVEIDNWPGDRPLIAG
CCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEECCCCCCCCEEEC
GSVIVGPMGDVLAGPLRGEAGLLTAEIDTEELVRARYDYDVVGHYARPDVFELSVDERAK
CCEEECCCHHHHCCCCCCCCCEEEEECCHHHHHHHHCCHHHCCCCCCCCEEEECCCCCCC
PGVRFTT
CCCEECC
>Mature Secondary Structure 
PKSIVAALQIGALPGGKAETLEQILSWETAIIESGAALVVMPEALLGGYPKGEGFGTQL
CCHHEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCCCCCC
GYRLPEGREAYARYFANAIDVPGAETEALAGLSARTGANLVIGVIERAGSTLHCTALYFD
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCEEEEEHHHCCCEEEEEEEEEC
PQAGLVAKHRKLMPTGTERLIWGKGDGSTLPVLDTQVGKLGAVICWENMMPLLRTAMYAK
CCCCHHHHHHCCCCCCCCEEEEECCCCCCCCEECCCCCHHEEEEEHHHHHHHHHHHHHHC
GIEVWCAPTVDEREMWQVSMRHIAHEGRCFVVSACQVQASPNDLGVEIDNWPGDRPLIAG
CCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEECCCCCCCCEEEC
GSVIVGPMGDVLAGPLRGEAGLLTAEIDTEELVRARYDYDVVGHYARPDVFELSVDERAK
CCEEECCCHHHHCCCCCCCCCEEEEECCHHHHHHHHCCHHHCCCCCCCCEEEECCCCCCC
PGVRFTT
CCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]