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The map label for this gene is potF [H]

Identifier: 77458800

GI number: 77458800

Start: 2952473

End: 2953564

Strand: Direct

Name: potF [H]

Synonym: Pfl01_2575

Alternate gene names: 77458800

Gene position: 2952473-2953564 (Clockwise)

Preceding gene: 77458796

Following gene: 77458801

Centisome position: 45.86

GC content: 62.09

Gene sequence:

>1092_bases
ATGGCTCCCGTCTTCAAGCTGTGTTTGCCCGCTTTATTACTTACCGTCTCCATCGCCGCCCAGGCCGAAGAGAAAACCCT
CAATCTCTACAGCTGGGCTGATTACGTGGCGCCTGACACCTTGCAGCGCTTCGAAAAGGAAACCGGCATTCACGTGCGCT
ACGACACCTTCGATACGTCCGAGGTGCTGGAAACCAAGCTGCTCACCGGCGGCAGCGGTTACGACGTAGTGGTGCCTTCG
TCCAGCGTGCTGGCCCGTGGCCTGGCCGCAGGAGCGCTCAAGGAAATTCCCCACGAAGGGCTCAAGGGTTACGCCAATCT
CGATCCCGATCTGCTGGAAAAACTGGCGGCGGTCGATCCCGGCAACCGCTACGGCGTGCCGTACACCTGGGGCACCCTCG
GCCTGGGCATGAACGTCGAAGCGGTCAAACAGCGGCTGCCGGATGTGCCGCTCAACAGCCTTGATCTGCTGTTCAGGCCT
GAATACGCCAGCAAACTCAAGGATTGCGGGATCGCCATTCTGGACTCGCCGCAGGAAGTGATCGGCCTGGCGCTGCACTA
CCTCGGTAAGGATCCCTACAGCACCGACAAGAGCGATCTGGCCGCCGCCGAAGCCCTGTTGCAGAAGTTGCAGCCCTCGG
TGCTGTACGTCGCCACCGGCCGGCAGATCAACGATCTGGCCAACGGCAGCGTGTGTCTGGCGCTGACCTACAACGGTGAC
GCGAGCATGGCCGCCGACCAGGCGCGCAAGGCCAACAAGCCGTTTGAGGTCGCGTACCGGATTCCCAAGGAAGGCACGCT
GGTGTGGCAGGACAATCTCGCCATTCCTAAAGACGCGCCGCACCCGGAAGCTGCACGCACCTTCATCGAGTTCATGTTGC
GCCCCGAATCCGTGGCGGCGCTGACCAATACGCTGTTCTTCGCCACCGCCAACCAGGCCGCCACGCCGCTGGTGGATGAA
GCGGTGCGCAACGATCCGGACATTTTCCCGAAACCCGAAGTGCGCGAGCGGTTGTACGCCGACCGCAGCATGAGCCTCAA
GGACATGCGCCAGCGCACGCGCTTGTGGACCACTTTCCGTAGCCGCCAATAG

Upstream 100 bases:

>100_bases
TCACATTAATCCGGGATAAAGTCAGGTTAACAAACTTGATATTTCTCGAATGTATCCGACGGCGCATCCTGCCACCACTT
CCTTTAGTCAGGTGTTCGTC

Downstream 100 bases:

>100_bases
CCCTTCATCACAACAGGAACACACCAATGGACGTGCCCATGCAAAACGATCAGGCCCTGACCCGTGACAGCCTTTTTGGC
ACCGCTGCTGAAAGCACCTA

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAPVFKLCLPALLLTVSIAAQAEEKTLNLYSWADYVAPDTLQRFEKETGIHVRYDTFDTSEVLETKLLTGGSGYDVVVPS
SSVLARGLAAGALKEIPHEGLKGYANLDPDLLEKLAAVDPGNRYGVPYTWGTLGLGMNVEAVKQRLPDVPLNSLDLLFRP
EYASKLKDCGIAILDSPQEVIGLALHYLGKDPYSTDKSDLAAAEALLQKLQPSVLYVATGRQINDLANGSVCLALTYNGD
ASMAADQARKANKPFEVAYRIPKEGTLVWQDNLAIPKDAPHPEAARTFIEFMLRPESVAALTNTLFFATANQAATPLVDE
AVRNDPDIFPKPEVRERLYADRSMSLKDMRQRTRLWTTFRSRQ

Sequences:

>Translated_363_residues
MAPVFKLCLPALLLTVSIAAQAEEKTLNLYSWADYVAPDTLQRFEKETGIHVRYDTFDTSEVLETKLLTGGSGYDVVVPS
SSVLARGLAAGALKEIPHEGLKGYANLDPDLLEKLAAVDPGNRYGVPYTWGTLGLGMNVEAVKQRLPDVPLNSLDLLFRP
EYASKLKDCGIAILDSPQEVIGLALHYLGKDPYSTDKSDLAAAEALLQKLQPSVLYVATGRQINDLANGSVCLALTYNGD
ASMAADQARKANKPFEVAYRIPKEGTLVWQDNLAIPKDAPHPEAARTFIEFMLRPESVAALTNTLFFATANQAATPLVDE
AVRNDPDIFPKPEVRERLYADRSMSLKDMRQRTRLWTTFRSRQ
>Mature_362_residues
APVFKLCLPALLLTVSIAAQAEEKTLNLYSWADYVAPDTLQRFEKETGIHVRYDTFDTSEVLETKLLTGGSGYDVVVPSS
SVLARGLAAGALKEIPHEGLKGYANLDPDLLEKLAAVDPGNRYGVPYTWGTLGLGMNVEAVKQRLPDVPLNSLDLLFRPE
YASKLKDCGIAILDSPQEVIGLALHYLGKDPYSTDKSDLAAAEALLQKLQPSVLYVATGRQINDLANGSVCLALTYNGDA
SMAADQARKANKPFEVAYRIPKEGTLVWQDNLAIPKDAPHPEAARTFIEFMLRPESVAALTNTLFFATANQAATPLVDEA
VRNDPDIFPKPEVRERLYADRSMSLKDMRQRTRLWTTFRSRQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=352, Percent_Identity=45.4545454545455, Blast_Score=327, Evalue=9e-91,
Organism=Escherichia coli, GI1787367, Length=320, Percent_Identity=35.9375, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787711, Length=349, Percent_Identity=27.2206303724928, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39943; Mature: 39812

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPVFKLCLPALLLTVSIAAQAEEKTLNLYSWADYVAPDTLQRFEKETGIHVRYDTFDTS
CCHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHCCHHHHHHHHHHCCCEEEECCCCHH
EVLETKLLTGGSGYDVVVPSSSVLARGLAAGALKEIPHEGLKGYANLDPDLLEKLAAVDP
HHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHCCC
GNRYGVPYTWGTLGLGMNVEAVKQRLPDVPLNSLDLLFRPEYASKLKDCGIAILDSPQEV
CCCCCCCEEHHHHCCCCCHHHHHHHCCCCCCCCCCEEECCHHHHHHHHCCEEEECCHHHH
IGLALHYLGKDPYSTDKSDLAAAEALLQKLQPSVLYVATGRQINDLANGSVCLALTYNGD
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCEEEEEEECCC
ASMAADQARKANKPFEVAYRIPKEGTLVWQDNLAIPKDAPHPEAARTFIEFMLRPESVAA
CHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
LTNTLFFATANQAATPLVDEAVRNDPDIFPKPEVRERLYADRSMSLKDMRQRTRLWTTFR
HHHHHEEEECCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SRQ
CCC
>Mature Secondary Structure 
APVFKLCLPALLLTVSIAAQAEEKTLNLYSWADYVAPDTLQRFEKETGIHVRYDTFDTS
CHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHCCHHHHHHHHHHCCCEEEECCCCHH
EVLETKLLTGGSGYDVVVPSSSVLARGLAAGALKEIPHEGLKGYANLDPDLLEKLAAVDP
HHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHCCC
GNRYGVPYTWGTLGLGMNVEAVKQRLPDVPLNSLDLLFRPEYASKLKDCGIAILDSPQEV
CCCCCCCEEHHHHCCCCCHHHHHHHCCCCCCCCCCEEECCHHHHHHHHCCEEEECCHHHH
IGLALHYLGKDPYSTDKSDLAAAEALLQKLQPSVLYVATGRQINDLANGSVCLALTYNGD
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCEEEEEEECCC
ASMAADQARKANKPFEVAYRIPKEGTLVWQDNLAIPKDAPHPEAARTFIEFMLRPESVAA
CHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
LTNTLFFATANQAATPLVDEAVRNDPDIFPKPEVRERLYADRSMSLKDMRQRTRLWTTFR
HHHHHEEEECCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SRQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]