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The map label for this gene is yafC [C]

Identifier: 77458675

GI number: 77458675

Start: 2803643

End: 2804560

Strand: Direct

Name: yafC [C]

Synonym: Pfl01_2450

Alternate gene names: 77458675

Gene position: 2803643-2804560 (Clockwise)

Preceding gene: 77458674

Following gene: 77458679

Centisome position: 43.55

GC content: 61.33

Gene sequence:

>918_bases
ATGGACAAGTTGCTGGCACTGAAGATGTTCGTTGAAACCGTCCGTTGCGGTGGTTATTCCTCGGCGGCGCGCAAACTCGG
CATCTCCACCTCTTCGGTGACACGCCAGGTGGCAGGGCTGGAAAACGAATTGGGCGCCAGCCTGCTCAACCGCACGACAC
GCAACACCAGCGTGACGGTCGCCGGCCAAACCTATTTCGAGAAAGCGGTGGCCATTCTCGATGCCATCGATGAGGCCGAC
GCGGTTGTCGCCGACCGTGGCAGCGATGCGCAGGGACGCCTGCGCATCAGTGTGCCGGTGGAATTCGGCCGACAGATCAT
TGCACCGCAACTGGGGGAATTGCTCGACCGCCATCCGGGGCTTGAAATCAGTGTGTCGTTGAGCGATCAGGTCAGCGATC
TGCTGAGTGAGCAGATCGATGTCTCGGTGCGCCTGGGATCATCGGTGGTCAGTGACGATATCGTCAGCAAGCGCGTCGGC
GGATTCCAGCGCTGGATTGTGGCGAGTCCGGATTACCTGAGCGGTCACGCTGTGCCGGCGCATCCGCGAGACTTGCTCGA
GCATCAATGCCTGCGCTTCGACTACGCTGGTTCGCACCAGCACTGGACGTTCCAGGGCGACGAGGAAACCATCCAGCTCA
ATGTGCATGGCCGCCTGCAAAGCAACAACGCCGATATCCTGCGCGAAGCGGCGGTGGCGGGGCGCGGGGTGGCCCTGCTG
GCGGACTGGCTGGTGCGCGCCGATGTCGAGGCCGGTCGGTTGACGCGGCTGCTGGAGGGGTATGAAGTCAACCCCGGTAA
TGCGAGCTGCTGCATCAATGCGTTGTATCTGCCCAATCACCGGGGCTCCAGTCGTATCAATGTGTTCATCGACTTTCTGG
AAGACATTCTGGCAGCGGAGCCATCGACTCAACGCTAG

Upstream 100 bases:

>100_bases
ACCGCAGGCACCGCTGAGTCCGATGAAGCACGGTCCTACAAGACGATCGGCGAACGGTGATGCACAATCATCACCGTTTC
CTTTCGATCGGGCTCCACTT

Downstream 100 bases:

>100_bases
AGTGCCGTTTATTGCCGCCCGACGCTTTGTGAGTTCTGCATAAAAAAGCGCCCGCCAAAAAAACCGTGGCGGGCGCCTGG
GAATGCGCAGCGATAGGGAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MDKLLALKMFVETVRCGGYSSAARKLGISTSSVTRQVAGLENELGASLLNRTTRNTSVTVAGQTYFEKAVAILDAIDEAD
AVVADRGSDAQGRLRISVPVEFGRQIIAPQLGELLDRHPGLEISVSLSDQVSDLLSEQIDVSVRLGSSVVSDDIVSKRVG
GFQRWIVASPDYLSGHAVPAHPRDLLEHQCLRFDYAGSHQHWTFQGDEETIQLNVHGRLQSNNADILREAAVAGRGVALL
ADWLVRADVEAGRLTRLLEGYEVNPGNASCCINALYLPNHRGSSRINVFIDFLEDILAAEPSTQR

Sequences:

>Translated_305_residues
MDKLLALKMFVETVRCGGYSSAARKLGISTSSVTRQVAGLENELGASLLNRTTRNTSVTVAGQTYFEKAVAILDAIDEAD
AVVADRGSDAQGRLRISVPVEFGRQIIAPQLGELLDRHPGLEISVSLSDQVSDLLSEQIDVSVRLGSSVVSDDIVSKRVG
GFQRWIVASPDYLSGHAVPAHPRDLLEHQCLRFDYAGSHQHWTFQGDEETIQLNVHGRLQSNNADILREAAVAGRGVALL
ADWLVRADVEAGRLTRLLEGYEVNPGNASCCINALYLPNHRGSSRINVFIDFLEDILAAEPSTQR
>Mature_305_residues
MDKLLALKMFVETVRCGGYSSAARKLGISTSSVTRQVAGLENELGASLLNRTTRNTSVTVAGQTYFEKAVAILDAIDEAD
AVVADRGSDAQGRLRISVPVEFGRQIIAPQLGELLDRHPGLEISVSLSDQVSDLLSEQIDVSVRLGSSVVSDDIVSKRVG
GFQRWIVASPDYLSGHAVPAHPRDLLEHQCLRFDYAGSHQHWTFQGDEETIQLNVHGRLQSNNADILREAAVAGRGVALL
ADWLVRADVEAGRLTRLLEGYEVNPGNASCCINALYLPNHRGSSRINVFIDFLEDILAAEPSTQR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=30.8474576271186, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=30.6666666666667, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1789440, Length=288, Percent_Identity=30.5555555555556, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=29.5918367346939, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1789639, Length=295, Percent_Identity=26.4406779661017, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=29.3515358361775, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=27.681660899654, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789173, Length=307, Percent_Identity=28.3387622149837, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33172; Mature: 33172

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLLALKMFVETVRCGGYSSAARKLGISTSSVTRQVAGLENELGASLLNRTTRNTSVTV
CCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEE
AGQTYFEKAVAILDAIDEADAVVADRGSDAQGRLRISVPVEFGRQIIAPQLGELLDRHPG
ECHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCC
LEISVSLSDQVSDLLSEQIDVSVRLGSSVVSDDIVSKRVGGFQRWIVASPDYLSGHAVPA
CEEEEECHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCC
HPRDLLEHQCLRFDYAGSHQHWTFQGDEETIQLNVHGRLQSNNADILREAAVAGRGVALL
CHHHHHHHHHHEECCCCCCCEEEEECCCEEEEEEEEEEEECCCHHHHHHHHHCCCCHHHH
ADWLVRADVEAGRLTRLLEGYEVNPGNASCCINALYLPNHRGSSRINVFIDFLEDILAAE
HHHHHHCCCCHHHHHHHHCCEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCC
PSTQR
CCCCC
>Mature Secondary Structure
MDKLLALKMFVETVRCGGYSSAARKLGISTSSVTRQVAGLENELGASLLNRTTRNTSVTV
CCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEE
AGQTYFEKAVAILDAIDEADAVVADRGSDAQGRLRISVPVEFGRQIIAPQLGELLDRHPG
ECHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCC
LEISVSLSDQVSDLLSEQIDVSVRLGSSVVSDDIVSKRVGGFQRWIVASPDYLSGHAVPA
CEEEEECHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCC
HPRDLLEHQCLRFDYAGSHQHWTFQGDEETIQLNVHGRLQSNNADILREAAVAGRGVALL
CHHHHHHHHHHEECCCCCCCEEEEECCCEEEEEEEEEEEECCCHHHHHHHHHCCCCHHHH
ADWLVRADVEAGRLTRLLEGYEVNPGNASCCINALYLPNHRGSSRINVFIDFLEDILAAE
HHHHHHCCCCHHHHHHHHCCEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCC
PSTQR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]