Click here to switch to the map view.

The map label for this gene is eptA [H]

Identifier: 77457614

GI number: 77457614

Start: 1560187

End: 1561836

Strand: Direct

Name: eptA [H]

Synonym: Pfl01_1387

Alternate gene names: 77457614

Gene position: 1560187-1561836 (Clockwise)

Preceding gene: 77457611

Following gene: 77457615

Centisome position: 24.23

GC content: 57.21

Gene sequence:

>1650_bases
ATGCTCAAGTTAAAAGCCGTGCGCCCGGAATGGGTGACGTTGTTTGCCAGCGCCTTTCTTTTGGCCGGATTCAATTTCGT
GCTATGGCAACACCTGTTCGACATTACTACCGCGGACGGCAAAGGCATTGCCATGCGCATTGCGTTCGGGGTGATGATCT
TCGCGGCGTATAACATCGTGCTGACGTTGCTGGCGTTTCGACCCGTGTTGAAGCCGGTGTTGACGTTGTTGTTCATGGTC
AGTGCCGGCGTGGCTTATTTCATGAGCCAATACGGGGTCATGATCGATGCCGGCATGTTTCGCAACTTTGCCGAAACCAA
TGCCACGGAAGTTCGCGACCTGCTGTCTTTGAAGTTGCTCGCTTATATCTTTTTTCTGGGTGTCTTGCCGTCCTGGTTGT
TGTGGAAAGTTCCAGTCAACTATCGTCGTTGGCATCGCGAGTTATTAAGTAAAGTTGTGGTGAGTGTCGCGTCGGTTGCA
GTCATTGGCGGCGTGGCGCTGGCCAATTATCAAGGGCTGTCGTCGCTGTTTCGCAATCACCACGAAATTCGCTTGATGCT
GGTGCCGAGCAACTACATCGGCGCCTCGGCCGGTTATCTGCGTGAGCAGGTGGCCTCGGTGCATCAACCGTTCGTCACAA
TCGGCGAAGATGCCCAGCGCAACGCCGCCGTGCAGAACCATCCGCGCAAATCCCTGACGGTGCTGGTGGTGGGGGAGAGT
GCCCGGGCGGAAAACTTCGGCATCCTTGGTTATGGCCGCGACACCACACCGAAACTGGACAAGGAAGCCGGCCTGATCGC
TTTCACCGACGTGCATTCCTGCGGCACGGAAACCGCCGTGTCGGTGCCGTGCATGTTCTCCAACATGGGCCGCAAGAATT
ACGACGCCAGCAAGGCAAAGAATGAGGAAGGGTTGCTGGACGTGCTTAAACGGGCCGGGATCGACGTGATCTGGCGCGAC
AACCAGTCGGGCTGCAAAGGCACCTGCGATCGCGTCACCCTGCAGGATGTCAGCAATCTGAAAGACCCGGCGCTGTGCGC
CAACAGCGAATGCCGCGACGAAATCCTGTTGCAGGGCCTGCAAAGCTTCATCGATCACCTGGACAAGGACACCGTGCTGG
TCCTGCATCAGATGGGCAGCCACGGCCCGGAGTATTTCAAGCGTTATCCGAAGGAATACGAACACTTCACACCGGTGTGT
GAGAGCAACGCACTGAACAATTGCAGCCGCGAGAGCATCGTCAACGGTTACGACAATACGCTGGTGTACACCGACCATGT
GCTGTCGAGCCTGATCGATGTGCTGCGCAGCAATCAGGACAAGGTCGATACCGCCATGCTGTACCTGTCCGACCACGGCG
AGTCGTTGGGTGAGTACAACCTGTTCCTCCACGGCACGCCGTACATGCTGGCGCCGGAGCAGCAGAAACACGTGGCGATG
CTGGCCTGGTTCTCCGACAGCTATCAGAAGTCCTACTCGGTCGACACTCATTGCCTGCAAATGAGCCGCGACAAGCCATT
GAGTCAGGACAACCTGTTTCACTCGATGCTCGGTCTGCTGGAAGTCCAGAGCAAGGTCTACCAGCCGGATCTGGACATGT
TTGCCGGCTGCCGGGGCGCGGTGATCGACGGGGTGTTGGCCAAGGACTGA

Upstream 100 bases:

>100_bases
CAAAAAATTGATGGTTGCGTGCTTAAAGTTTGTGCGGTCTGAGTCAATGAAATTTTCACAATTGACGACGGCACTTCTAT
CAAGGAAATCGCCCCTCGCC

Downstream 100 bases:

>100_bases
GCCGGCAGACTCACTCTGTAAGACTATTCTTTCCCACAGGCAGGAGATTTCATCAAGCTCTTGCCCTGTAGAGGCGCGGA
AAGCGCCGGTGGCGATATAT

Product: phosphatidylethanolamine:Kdo2-lipid A phosphoethanolamine transferase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MLKLKAVRPEWVTLFASAFLLAGFNFVLWQHLFDITTADGKGIAMRIAFGVMIFAAYNIVLTLLAFRPVLKPVLTLLFMV
SAGVAYFMSQYGVMIDAGMFRNFAETNATEVRDLLSLKLLAYIFFLGVLPSWLLWKVPVNYRRWHRELLSKVVVSVASVA
VIGGVALANYQGLSSLFRNHHEIRLMLVPSNYIGASAGYLREQVASVHQPFVTIGEDAQRNAAVQNHPRKSLTVLVVGES
ARAENFGILGYGRDTTPKLDKEAGLIAFTDVHSCGTETAVSVPCMFSNMGRKNYDASKAKNEEGLLDVLKRAGIDVIWRD
NQSGCKGTCDRVTLQDVSNLKDPALCANSECRDEILLQGLQSFIDHLDKDTVLVLHQMGSHGPEYFKRYPKEYEHFTPVC
ESNALNNCSRESIVNGYDNTLVYTDHVLSSLIDVLRSNQDKVDTAMLYLSDHGESLGEYNLFLHGTPYMLAPEQQKHVAM
LAWFSDSYQKSYSVDTHCLQMSRDKPLSQDNLFHSMLGLLEVQSKVYQPDLDMFAGCRGAVIDGVLAKD

Sequences:

>Translated_549_residues
MLKLKAVRPEWVTLFASAFLLAGFNFVLWQHLFDITTADGKGIAMRIAFGVMIFAAYNIVLTLLAFRPVLKPVLTLLFMV
SAGVAYFMSQYGVMIDAGMFRNFAETNATEVRDLLSLKLLAYIFFLGVLPSWLLWKVPVNYRRWHRELLSKVVVSVASVA
VIGGVALANYQGLSSLFRNHHEIRLMLVPSNYIGASAGYLREQVASVHQPFVTIGEDAQRNAAVQNHPRKSLTVLVVGES
ARAENFGILGYGRDTTPKLDKEAGLIAFTDVHSCGTETAVSVPCMFSNMGRKNYDASKAKNEEGLLDVLKRAGIDVIWRD
NQSGCKGTCDRVTLQDVSNLKDPALCANSECRDEILLQGLQSFIDHLDKDTVLVLHQMGSHGPEYFKRYPKEYEHFTPVC
ESNALNNCSRESIVNGYDNTLVYTDHVLSSLIDVLRSNQDKVDTAMLYLSDHGESLGEYNLFLHGTPYMLAPEQQKHVAM
LAWFSDSYQKSYSVDTHCLQMSRDKPLSQDNLFHSMLGLLEVQSKVYQPDLDMFAGCRGAVIDGVLAKD
>Mature_549_residues
MLKLKAVRPEWVTLFASAFLLAGFNFVLWQHLFDITTADGKGIAMRIAFGVMIFAAYNIVLTLLAFRPVLKPVLTLLFMV
SAGVAYFMSQYGVMIDAGMFRNFAETNATEVRDLLSLKLLAYIFFLGVLPSWLLWKVPVNYRRWHRELLSKVVVSVASVA
VIGGVALANYQGLSSLFRNHHEIRLMLVPSNYIGASAGYLREQVASVHQPFVTIGEDAQRNAAVQNHPRKSLTVLVVGES
ARAENFGILGYGRDTTPKLDKEAGLIAFTDVHSCGTETAVSVPCMFSNMGRKNYDASKAKNEEGLLDVLKRAGIDVIWRD
NQSGCKGTCDRVTLQDVSNLKDPALCANSECRDEILLQGLQSFIDHLDKDTVLVLHQMGSHGPEYFKRYPKEYEHFTPVC
ESNALNNCSRESIVNGYDNTLVYTDHVLSSLIDVLRSNQDKVDTAMLYLSDHGESLGEYNLFLHGTPYMLAPEQQKHVAM
LAWFSDSYQKSYSVDTHCLQMSRDKPLSQDNLFHSMLGLLEVQSKVYQPDLDMFAGCRGAVIDGVLAKD

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=498, Percent_Identity=45.7831325301205, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI87082286, Length=483, Percent_Identity=26.5010351966874, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI87082223, Length=256, Percent_Identity=28.125, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1790392, Length=317, Percent_Identity=25.5520504731861, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787035, Length=238, Percent_Identity=28.1512605042017, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61242; Mature: 61242

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLKAVRPEWVTLFASAFLLAGFNFVLWQHLFDITTADGKGIAMRIAFGVMIFAAYNIV
CCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHH
LTLLAFRPVLKPVLTLLFMVSAGVAYFMSQYGVMIDAGMFRNFAETNATEVRDLLSLKLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCHHHHHHHHHHHHH
AYIFFLGVLPSWLLWKVPVNYRRWHRELLSKVVVSVASVAVIGGVALANYQGLSSLFRNH
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HEIRLMLVPSNYIGASAGYLREQVASVHQPFVTIGEDAQRNAAVQNHPRKSLTVLVVGES
CEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEECCCHHHCCHHHCCCCCCEEEEEECCC
ARAENFGILGYGRDTTPKLDKEAGLIAFTDVHSCGTETAVSVPCMFSNMGRKNYDASKAK
CCCCCCEEEEECCCCCCCCCCCCCEEEEECCHHCCCCCCEECCHHHHHCCCCCCCCHHCC
NEEGLLDVLKRAGIDVIWRDNQSGCKGTCDRVTLQDVSNLKDPALCANSECRDEILLQGL
CCCHHHHHHHHCCCEEEEECCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCHHHHHHHHHH
QSFIDHLDKDTVLVLHQMGSHGPEYFKRYPKEYEHFTPVCESNALNNCSRESIVNGYDNT
HHHHHHCCCCCEEEEHHHCCCCHHHHHHCCHHHHHHCCCHHCCCCCCCCHHHHHCCCCCC
LVYTDHVLSSLIDVLRSNQDKVDTAMLYLSDHGESLGEYNLFLHGTPYMLAPEQQKHVAM
EEEHHHHHHHHHHHHHCCCCCHHHEEEEEECCCCCCCCEEEEEECCCCEECCCCCCCEEE
LAWFSDSYQKSYSVDTHCLQMSRDKPLSQDNLFHSMLGLLEVQSKVYQPDLDMFAGCRGA
EEEHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHH
VIDGVLAKD
HHCCHHCCC
>Mature Secondary Structure
MLKLKAVRPEWVTLFASAFLLAGFNFVLWQHLFDITTADGKGIAMRIAFGVMIFAAYNIV
CCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHH
LTLLAFRPVLKPVLTLLFMVSAGVAYFMSQYGVMIDAGMFRNFAETNATEVRDLLSLKLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCHHHHHHHHHHHHH
AYIFFLGVLPSWLLWKVPVNYRRWHRELLSKVVVSVASVAVIGGVALANYQGLSSLFRNH
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HEIRLMLVPSNYIGASAGYLREQVASVHQPFVTIGEDAQRNAAVQNHPRKSLTVLVVGES
CEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEECCCHHHCCHHHCCCCCCEEEEEECCC
ARAENFGILGYGRDTTPKLDKEAGLIAFTDVHSCGTETAVSVPCMFSNMGRKNYDASKAK
CCCCCCEEEEECCCCCCCCCCCCCEEEEECCHHCCCCCCEECCHHHHHCCCCCCCCHHCC
NEEGLLDVLKRAGIDVIWRDNQSGCKGTCDRVTLQDVSNLKDPALCANSECRDEILLQGL
CCCHHHHHHHHCCCEEEEECCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCHHHHHHHHHH
QSFIDHLDKDTVLVLHQMGSHGPEYFKRYPKEYEHFTPVCESNALNNCSRESIVNGYDNT
HHHHHHCCCCCEEEEHHHCCCCHHHHHHCCHHHHHHCCCHHCCCCCCCCHHHHHCCCCCC
LVYTDHVLSSLIDVLRSNQDKVDTAMLYLSDHGESLGEYNLFLHGTPYMLAPEQQKHVAM
EEEHHHHHHHHHHHHHCCCCCHHHEEEEEECCCCCCCCEEEEEECCCCEECCCCCCCEEE
LAWFSDSYQKSYSVDTHCLQMSRDKPLSQDNLFHSMLGLLEVQSKVYQPDLDMFAGCRGA
EEEHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHH
VIDGVLAKD
HHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]