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The map label for this gene is hipO [H]

Identifier: 77457518

GI number: 77457518

Start: 1465978

End: 1467153

Strand: Direct

Name: hipO [H]

Synonym: Pfl01_1291

Alternate gene names: 77457518

Gene position: 1465978-1467153 (Clockwise)

Preceding gene: 77457517

Following gene: 77457519

Centisome position: 22.77

GC content: 65.73

Gene sequence:

>1176_bases
ATGCCCCGACATCAGCACATTCTGGCCTGGCTCAACGATGTGGCCAGCGACCTGCACGCCACCCGCCAGGACATTCACGC
CCACCCTGAACTCGGTTTCGAAGAAAGCCGCACCTCGGCGCTGGTCGCCAGGTCGCTCGAAGAGTGGGGCTATGAAGTCC
ACACCGGCGTCGGCAAGACCGGCGTGGTCGGCGTGCTGCGCAACGGCAGCAGCACGCGCAAACTCGGGCTGCGGGCCGAC
ATGGATGCGCTGCCGATCATCGAAAATACCGGTGTCGCCTACAGCAGCCGGCATCAAGGCTGCATGCACGCCTGCGGCCA
CGACGGGCACACCGCGATGTTGCTCGGTGCGGCGCGTTATCTGGCCGCGACCCGTCAGTTCGACGGCACCCTGACGCTGA
TTTTTCAGCCCGCCGAAGAGGGCCAGGGCGGGGCGGAAGCGATGCTCGCCGATGGCCTGCTCGAACGTTTTCCGTGCGAT
GCGCTGTTCGGCATGCACAACATGCCGGGCCTGCCAGCGGGGCACCTGGGCTTTCGCGAAGGGCCGATGATGGCTTCGCA
GGATCTGCTCACGGTGACCATCGAAGGTGTCGGCGGCCACGGCTCGATGCCGCATCTGGCGGTGGATCCGCTGGTTGCCG
CAGCCAGTGTGGTGATGGCTTTGCAAACCGTGGTCGCGCGCAACATCGATGCGCAGCAGGCGGCGGTGGTAACGGTCGGC
GCATTGCAGGCCGGCGAAGCGGCGAACGTGATTCCGCAACAGGCGATCCTGCGCCTGAGCTTGCGTGCGTTGAATGCCGA
AGTCCGTGTTCAGACACTGGACCGCGTACGCTCGATCATCGAAGCGCAGGCCGAGAGCTTCGGCTGCACCTCGACCATCG
AACACCGCCCGGCCTACCCGGTGCTGGTCAACCACGCCGCCGAAACCGAGTTCGCCCGTCAGGTCGGCGTCGAACTGGTC
GGTACCGACGCCGTCGATGGCAACACTCCGAAACTGATGGGCAGCGAAGACTTCGCCTGGATGCTCCAGCGCTGCCCCGG
CGCCTATCTGTTCATCGGCAACGGCGTGGCGCGGCCGATGGTGCATAACCCGGCCTACGACTTCAACGATGACATCCTGC
TGACCGGCGCGGCGTATTGGGGCGCGCTCACCGAGAGCTGGCTCAAGCCGGCCTGA

Upstream 100 bases:

>100_bases
TGTCTCGCGGTTATCTGGCGGTGAGCACGACCTGCCGTTCACGTGTCGTGAAACAACACCGAACAACAAGACCGGGAACA
CCCGCGCGAGGAGATCCGTC

Downstream 100 bases:

>100_bases
TTTCTTCACTTTTTGCTGCCGCTGGAACGCAGGTGCGCACGCGCCTGCGTGGTCAACCCACAGCTTCTGGAGAATCCCCA
TGCAGACTTCGAACGCAGGC

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MPRHQHILAWLNDVASDLHATRQDIHAHPELGFEESRTSALVARSLEEWGYEVHTGVGKTGVVGVLRNGSSTRKLGLRAD
MDALPIIENTGVAYSSRHQGCMHACGHDGHTAMLLGAARYLAATRQFDGTLTLIFQPAEEGQGGAEAMLADGLLERFPCD
ALFGMHNMPGLPAGHLGFREGPMMASQDLLTVTIEGVGGHGSMPHLAVDPLVAAASVVMALQTVVARNIDAQQAAVVTVG
ALQAGEAANVIPQQAILRLSLRALNAEVRVQTLDRVRSIIEAQAESFGCTSTIEHRPAYPVLVNHAAETEFARQVGVELV
GTDAVDGNTPKLMGSEDFAWMLQRCPGAYLFIGNGVARPMVHNPAYDFNDDILLTGAAYWGALTESWLKPA

Sequences:

>Translated_391_residues
MPRHQHILAWLNDVASDLHATRQDIHAHPELGFEESRTSALVARSLEEWGYEVHTGVGKTGVVGVLRNGSSTRKLGLRAD
MDALPIIENTGVAYSSRHQGCMHACGHDGHTAMLLGAARYLAATRQFDGTLTLIFQPAEEGQGGAEAMLADGLLERFPCD
ALFGMHNMPGLPAGHLGFREGPMMASQDLLTVTIEGVGGHGSMPHLAVDPLVAAASVVMALQTVVARNIDAQQAAVVTVG
ALQAGEAANVIPQQAILRLSLRALNAEVRVQTLDRVRSIIEAQAESFGCTSTIEHRPAYPVLVNHAAETEFARQVGVELV
GTDAVDGNTPKLMGSEDFAWMLQRCPGAYLFIGNGVARPMVHNPAYDFNDDILLTGAAYWGALTESWLKPA
>Mature_390_residues
PRHQHILAWLNDVASDLHATRQDIHAHPELGFEESRTSALVARSLEEWGYEVHTGVGKTGVVGVLRNGSSTRKLGLRADM
DALPIIENTGVAYSSRHQGCMHACGHDGHTAMLLGAARYLAATRQFDGTLTLIFQPAEEGQGGAEAMLADGLLERFPCDA
LFGMHNMPGLPAGHLGFREGPMMASQDLLTVTIEGVGGHGSMPHLAVDPLVAAASVVMALQTVVARNIDAQQAAVVTVGA
LQAGEAANVIPQQAILRLSLRALNAEVRVQTLDRVRSIIEAQAESFGCTSTIEHRPAYPVLVNHAAETEFARQVGVELVG
TDAVDGNTPKLMGSEDFAWMLQRCPGAYLFIGNGVARPMVHNPAYDFNDDILLTGAAYWGALTESWLKPA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=420, Percent_Identity=28.0952380952381, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41789; Mature: 41658

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRHQHILAWLNDVASDLHATRQDIHAHPELGFEESRTSALVARSLEEWGYEVHTGVGKT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCC
GVVGVLRNGSSTRKLGLRADMDALPIIENTGVAYSSRHQGCMHACGHDGHTAMLLGAARY
CEEEEEECCCCCCEECCCCCCCCCCEEECCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHH
LAATRQFDGTLTLIFQPAEEGQGGAEAMLADGLLERFPCDALFGMHNMPGLPAGHLGFRE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCC
GPMMASQDLLTVTIEGVGGHGSMPHLAVDPLVAAASVVMALQTVVARNIDAQQAAVVTVG
CCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEE
ALQAGEAANVIPQQAILRLSLRALNAEVRVQTLDRVRSIIEAQAESFGCTSTIEHRPAYP
CCCCCCHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VLVNHAAETEFARQVGVELVGTDAVDGNTPKLMGSEDFAWMLQRCPGAYLFIGNGVARPM
EEECCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCC
VHNPAYDFNDDILLTGAAYWGALTESWLKPA
CCCCCCCCCCCEEEECHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PRHQHILAWLNDVASDLHATRQDIHAHPELGFEESRTSALVARSLEEWGYEVHTGVGKT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCC
GVVGVLRNGSSTRKLGLRADMDALPIIENTGVAYSSRHQGCMHACGHDGHTAMLLGAARY
CEEEEEECCCCCCEECCCCCCCCCCEEECCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHH
LAATRQFDGTLTLIFQPAEEGQGGAEAMLADGLLERFPCDALFGMHNMPGLPAGHLGFRE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCC
GPMMASQDLLTVTIEGVGGHGSMPHLAVDPLVAAASVVMALQTVVARNIDAQQAAVVTVG
CCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEE
ALQAGEAANVIPQQAILRLSLRALNAEVRVQTLDRVRSIIEAQAESFGCTSTIEHRPAYP
CCCCCCHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VLVNHAAETEFARQVGVELVGTDAVDGNTPKLMGSEDFAWMLQRCPGAYLFIGNGVARPM
EEECCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCC
VHNPAYDFNDDILLTGAAYWGALTESWLKPA
CCCCCCCCCCCEEEECHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]