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The map label for this gene is citH [H]

Identifier: 77457519

GI number: 77457519

Start: 1467233

End: 1468543

Strand: Direct

Name: citH [H]

Synonym: Pfl01_1292

Alternate gene names: 77457519

Gene position: 1467233-1468543 (Clockwise)

Preceding gene: 77457518

Following gene: 77457520

Centisome position: 22.79

GC content: 63.08

Gene sequence:

>1311_bases
ATGCAGACTTCGAACGCAGGCGTTTCGCGCACCCGGCAAGTGGTCGCCGCCGTCATCGGCAACGCCCTGGAATGGTACGA
CTTCATCGTTTATGGCTTTCTGGCGAGCATCATCGCCCGGCAATTTTTCCCGTCCGACGACGACTACGCTTCGCTGCTGA
TGGCGCTGGCGACTTTCGGCGTCGGCTTTTTCATGCGGCCGGTGGGCGGCATCCTGCTCGGCATCTACTCCGACCGCAAA
GGTCGCAAAGCCGCGATGCAAATGATCATCCGCCTGATGACCGTGTCCATCGCCCTGATCGCGTTCGCGCCGAACTACGC
CGCCATCGGCATGGGCGCGCCGCTGCTGATCGTGGTGGCGCGGATGCTTCAGGGTTTTGCCACGGGCGGTGAATACGCCA
GCGCCACGGCGTTTCTGGTGGAGAGCGCGCCGGCCCATCGCAAGGGCTTGTACGGTTCGTGGCAACTGGTCGGGCAATGC
CTGGCGGTGTTCAGCGGCGCGGCGATGGTGGCGCTGGTCACGCACCTGTTTTCCCCCGAGGCGCTGGATAGTTGGGGCTG
GCGGATTCCGTTTGTGCTCGGCCTGTTGATCGGCCCGGTGGGCTTGTGGATTCGCAAGCACATGGAGGAACCGGAAGAGT
TTCTCGAAGCGCGCAAGAAAGCCAAAGGCGCCGCGCCGGGCCTGTGGCAGGTACTGCGCGAACATCGCCGCAGCCTGTTG
GTGTCGATGGGCCTGGCGTGCGGGGCGACGGTGTCGTTTTACGTGGTGCTGGTGAACATGCCGACCTTCGCTCACAAGAA
CCTCGGCCTGCCGCTGGATCAGGTGTTGCTGGTGCAGATGCTCGCGGTGGGGTTGATGACCGTGGTGATTCCGTTCTCCG
GCGCACTGTCGGATCGCCTCGGTCGGCGGCCGGTGCTGATGGCTTTCACCCTGGCGTTTTTCGTCATGGTCTATCCGCTT
TACGTGTGGGTCGCGACGGCGCCGTCGATCGAGCGTTTGCTGGTGATGCAACTGCTGCTGTGCACGGCGATTGGGGGGGT
CTTCGGCCCGGCCCCGACGGCATTGGCCGAGCAGTTTCCGATTGAGGTGCGCTCCACCGGTGTGTCGGTGGCCTACAACG
TAGCGGTGATGGTGTTCGGTGGTTTCGCGCCGCTGATCGTCACCTGGCTGAGCAAAGTTCTGAACACCCCGGTGGCGCCG
TCGTTCTATGTCTTGTTCGCTTGCCTGCTGACCCTGCTCGGTACTTACTGTTTGCAGGAGGCACCACGCGCGAAGAAATC
CGTTGCGCTCAACCTGGGAGTGAAACCGTGA

Upstream 100 bases:

>100_bases
AGCTGGCTCAAGCCGGCCTGATTTCTTCACTTTTTGCTGCCGCTGGAACGCAGGTGCGCACGCGCCTGCGTGGTCAACCC
ACAGCTTCTGGAGAATCCCC

Downstream 100 bases:

>100_bases
GTCCGTTAGCCATTGATCCGATTGTCGCCCTCGACGCCGATGCGCTGTCCCGGGCGATCCATGCCCGCAAGGTGTCGTGC
CGCGAGGTGATGCAGGCCTA

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MQTSNAGVSRTRQVVAAVIGNALEWYDFIVYGFLASIIARQFFPSDDDYASLLMALATFGVGFFMRPVGGILLGIYSDRK
GRKAAMQMIIRLMTVSIALIAFAPNYAAIGMGAPLLIVVARMLQGFATGGEYASATAFLVESAPAHRKGLYGSWQLVGQC
LAVFSGAAMVALVTHLFSPEALDSWGWRIPFVLGLLIGPVGLWIRKHMEEPEEFLEARKKAKGAAPGLWQVLREHRRSLL
VSMGLACGATVSFYVVLVNMPTFAHKNLGLPLDQVLLVQMLAVGLMTVVIPFSGALSDRLGRRPVLMAFTLAFFVMVYPL
YVWVATAPSIERLLVMQLLLCTAIGGVFGPAPTALAEQFPIEVRSTGVSVAYNVAVMVFGGFAPLIVTWLSKVLNTPVAP
SFYVLFACLLTLLGTYCLQEAPRAKKSVALNLGVKP

Sequences:

>Translated_436_residues
MQTSNAGVSRTRQVVAAVIGNALEWYDFIVYGFLASIIARQFFPSDDDYASLLMALATFGVGFFMRPVGGILLGIYSDRK
GRKAAMQMIIRLMTVSIALIAFAPNYAAIGMGAPLLIVVARMLQGFATGGEYASATAFLVESAPAHRKGLYGSWQLVGQC
LAVFSGAAMVALVTHLFSPEALDSWGWRIPFVLGLLIGPVGLWIRKHMEEPEEFLEARKKAKGAAPGLWQVLREHRRSLL
VSMGLACGATVSFYVVLVNMPTFAHKNLGLPLDQVLLVQMLAVGLMTVVIPFSGALSDRLGRRPVLMAFTLAFFVMVYPL
YVWVATAPSIERLLVMQLLLCTAIGGVFGPAPTALAEQFPIEVRSTGVSVAYNVAVMVFGGFAPLIVTWLSKVLNTPVAP
SFYVLFACLLTLLGTYCLQEAPRAKKSVALNLGVKP
>Mature_436_residues
MQTSNAGVSRTRQVVAAVIGNALEWYDFIVYGFLASIIARQFFPSDDDYASLLMALATFGVGFFMRPVGGILLGIYSDRK
GRKAAMQMIIRLMTVSIALIAFAPNYAAIGMGAPLLIVVARMLQGFATGGEYASATAFLVESAPAHRKGLYGSWQLVGQC
LAVFSGAAMVALVTHLFSPEALDSWGWRIPFVLGLLIGPVGLWIRKHMEEPEEFLEARKKAKGAAPGLWQVLREHRRSLL
VSMGLACGATVSFYVVLVNMPTFAHKNLGLPLDQVLLVQMLAVGLMTVVIPFSGALSDRLGRRPVLMAFTLAFFVMVYPL
YVWVATAPSIERLLVMQLLLCTAIGGVFGPAPTALAEQFPIEVRSTGVSVAYNVAVMVFGGFAPLIVTWLSKVLNTPVAP
SFYVLFACLLTLLGTYCLQEAPRAKKSVALNLGVKP

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=420, Percent_Identity=33.0952380952381, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1788292, Length=424, Percent_Identity=33.0188679245283, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1788942, Length=430, Percent_Identity=31.6279069767442, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1789941, Length=391, Percent_Identity=34.7826086956522, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI87082404, Length=389, Percent_Identity=24.6786632390745, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082231, Length=467, Percent_Identity=25.4817987152034, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47091; Mature: 47091

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHEEEECCCCC
>Mature Secondary Structure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CCCHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]