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The map label for this gene is nylB' [H]

Identifier: 77457516

GI number: 77457516

Start: 1464456

End: 1465535

Strand: Direct

Name: nylB' [H]

Synonym: Pfl01_1289

Alternate gene names: 77457516

Gene position: 1464456-1465535 (Clockwise)

Preceding gene: 77457515

Following gene: 77457517

Centisome position: 22.75

GC content: 64.26

Gene sequence:

>1080_bases
ATGTTCAAAGGCCTGTCCCTGTTTCTGCTGCTGATCAGCTTCACGGTTCAGGCCGAACAATGGCCGGGCGAGCAATGGCC
GACCGGCCCGAAACTCACCAGCCCGGCCGTCGATGCATTGGAGGCCTACGCCTTCCCGACCCGCGATGACAGCACCCGAA
AAGGCATTCGCACCGACGCCTTGCTGGTGATCCGTGACGGCCAGCTCATCTACGAACGCTACGCCGCCGCGACCACCGCC
GACACCCCGCACCTGACCTGGTCGATCAGCAAAAGCCTGATGGCCACGGTGCTCGGCGTGGCGTACGGCGAAGGCCTGTT
CAAGCTCGATGACGCGGCGGCAACGTTCTACCCGGCGCTGGAAAAGCACCCGGCCATCACCCTCAAGGATCTGCTGCACT
GGGCCTCGGGCATCGACTGGCAGGAAGACTACGAATACGCCCCATTGAAATCCTCGGTGGTGGCGATGCTTTACACCCGT
GGTCACCGCGACATGGCCGCCTTCACCGCTAATCACGACAGCTACGCGCCAGCGGGGCAGGTGTTCCGTTATTCCAGCGG
CGACAGCAATCTGTTGGCCGCCGCACTGAAAACCATCGTCGGCCCACAGCGTTATCCGGACTATCCGTGGACTGCGCTTT
TCGATCCCTTGGGCATCCGCCATGTCGCCTGGGAAACCGACGCGACGGGCACCTTCGTCGCCTCGTCTTACGTTTATATG
AGCGCCCGGGATCTGGCGCGAATCGGCCTGCTGATGGAACGGGACGGACGCTGGAACGACCGGCAGTTGCTGCCCAAGGA
CTGGGTCGCCTTCAACCGCCAACCCTTCGCCGGCTACAAGGCCCATCAGGACGAAGCCGTGCCCGGCGGCCACTGGTGGC
TCAATCGTCCGGCAGACGGCGCGCCTTTGCCGTGGCCCGACGTACCGCCCGACACCTTCGCCGCCCTCGGCCACTGGGGC
CAGGCGCTGTACGTGATCCCGAGCGAAAAACTGGTGATCGTGCGCTACGCCGATGACCGCGACGGCAGCTATCGCCACAA
CGAATTGCTCAAGCGCGTGCTCGGGGCGGTGCGGCCATGA

Upstream 100 bases:

>100_bases
GCGCGGGCCAAACGCCTGGCCAAGGCGTTGGCGGCAAACCAAAACACATCTGTCATGAACGCCGGCTAGTCTTCTCAGGT
TCATTCCCAGAGCCCTCGCC

Downstream 100 bases:

>100_bases
AACGGTTCAGACGCATCCTGCTGGTGATGTTGGTCGTGTTGCTGGGCTGGGGTTGGCACGAGCGCGAAAACCTGTGGGCC
TTCCCCGACATCATCAGCGC

Product: beta-lactamase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII' [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MFKGLSLFLLLISFTVQAEQWPGEQWPTGPKLTSPAVDALEAYAFPTRDDSTRKGIRTDALLVIRDGQLIYERYAAATTA
DTPHLTWSISKSLMATVLGVAYGEGLFKLDDAAATFYPALEKHPAITLKDLLHWASGIDWQEDYEYAPLKSSVVAMLYTR
GHRDMAAFTANHDSYAPAGQVFRYSSGDSNLLAAALKTIVGPQRYPDYPWTALFDPLGIRHVAWETDATGTFVASSYVYM
SARDLARIGLLMERDGRWNDRQLLPKDWVAFNRQPFAGYKAHQDEAVPGGHWWLNRPADGAPLPWPDVPPDTFAALGHWG
QALYVIPSEKLVIVRYADDRDGSYRHNELLKRVLGAVRP

Sequences:

>Translated_359_residues
MFKGLSLFLLLISFTVQAEQWPGEQWPTGPKLTSPAVDALEAYAFPTRDDSTRKGIRTDALLVIRDGQLIYERYAAATTA
DTPHLTWSISKSLMATVLGVAYGEGLFKLDDAAATFYPALEKHPAITLKDLLHWASGIDWQEDYEYAPLKSSVVAMLYTR
GHRDMAAFTANHDSYAPAGQVFRYSSGDSNLLAAALKTIVGPQRYPDYPWTALFDPLGIRHVAWETDATGTFVASSYVYM
SARDLARIGLLMERDGRWNDRQLLPKDWVAFNRQPFAGYKAHQDEAVPGGHWWLNRPADGAPLPWPDVPPDTFAALGHWG
QALYVIPSEKLVIVRYADDRDGSYRHNELLKRVLGAVRP
>Mature_359_residues
MFKGLSLFLLLISFTVQAEQWPGEQWPTGPKLTSPAVDALEAYAFPTRDDSTRKGIRTDALLVIRDGQLIYERYAAATTA
DTPHLTWSISKSLMATVLGVAYGEGLFKLDDAAATFYPALEKHPAITLKDLLHWASGIDWQEDYEYAPLKSSVVAMLYTR
GHRDMAAFTANHDSYAPAGQVFRYSSGDSNLLAAALKTIVGPQRYPDYPWTALFDPLGIRHVAWETDATGTFVASSYVYM
SARDLARIGLLMERDGRWNDRQLLPKDWVAFNRQPFAGYKAHQDEAVPGGHWWLNRPADGAPLPWPDVPPDTFAALGHWG
QALYVIPSEKLVIVRYADDRDGSYRHNELLKRVLGAVRP

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 40130; Mature: 40130

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGLSLFLLLISFTVQAEQWPGEQWPTGPKLTSPAVDALEAYAFPTRDDSTRKGIRTDA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCCE
LLVIRDGQLIYERYAAATTADTPHLTWSISKSLMATVLGVAYGEGLFKLDDAAATFYPAL
EEEEECCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHH
EKHPAITLKDLLHWASGIDWQEDYEYAPLKSSVVAMLYTRGHRDMAAFTANHDSYAPAGQ
HCCCCEEHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
VFRYSSGDSNLLAAALKTIVGPQRYPDYPWTALFDPLGIRHVAWETDATGTFVASSYVYM
EEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEECCCCCCEEEEHHEEEE
SARDLARIGLLMERDGRWNDRQLLPKDWVAFNRQPFAGYKAHQDEAVPGGHWWLNRPADG
CHHHHHHHHHHEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCC
APLPWPDVPPDTFAALGHWGQALYVIPSEKLVIVRYADDRDGSYRHNELLKRVLGAVRP
CCCCCCCCCHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKGLSLFLLLISFTVQAEQWPGEQWPTGPKLTSPAVDALEAYAFPTRDDSTRKGIRTDA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCCE
LLVIRDGQLIYERYAAATTADTPHLTWSISKSLMATVLGVAYGEGLFKLDDAAATFYPAL
EEEEECCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHH
EKHPAITLKDLLHWASGIDWQEDYEYAPLKSSVVAMLYTRGHRDMAAFTANHDSYAPAGQ
HCCCCEEHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
VFRYSSGDSNLLAAALKTIVGPQRYPDYPWTALFDPLGIRHVAWETDATGTFVASSYVYM
EEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEECCCCCCEEEEHHEEEE
SARDLARIGLLMERDGRWNDRQLLPKDWVAFNRQPFAGYKAHQDEAVPGGHWWLNRPADG
CHHHHHHHHHHEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCC
APLPWPDVPPDTFAALGHWGQALYVIPSEKLVIVRYADDRDGSYRHNELLKRVLGAVRP
CCCCCCCCCHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 1879421 [H]