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The map label for this gene is pcaF [H]

Identifier: 77457496

GI number: 77457496

Start: 1442399

End: 1443604

Strand: Direct

Name: pcaF [H]

Synonym: Pfl01_1269

Alternate gene names: 77457496

Gene position: 1442399-1443604 (Clockwise)

Preceding gene: 77457495

Following gene: 77457497

Centisome position: 22.4

GC content: 64.43

Gene sequence:

>1206_bases
ATGATGCGCGACGTTTATATCTGCGATGCGATTCGTACCCCCATCGGTCGTTTTGGTGGCGGTCTGGCGGCGGTTCGCGC
CGACGACCTGGCCGCCGTGCCGATCAAGGCCCTGATGGAACGCAATCCGTCGGTGGACTGGAGCGCCATCGACGAGGTAT
TCCTCGGCTGCGCCAACCAGGCTGGCGAAGACAACCGTAACGTGGCGCGCATGGCGTTGCTGCTGGCCGGTCTGCCGGAG
AGCGTGCCGGGCGTCACTCTCAATCGCCTCTGCGCGTCGGGCATGGATGCCATCGGCACCGCGTTCCGCGCCATCGCCAG
CGGTGAAATGGAGCTGGCGATTGCCGGCGGTGTCGAGTCGATGTCCCGCGCACCGTTCGTGATGGGCAAGGCCGACGCGG
CGTTTTCGCGCAACATGAAACTGGAAGACACCACCATCGGCTGGCGTTTCATCAACCCGTTGATGAAGGCCCAGTACGGC
GTCGACGCGATGCCGCAGACCGCCGACAATGTTGCCGACGATTACGAAATTTCCCGCGAGGATCAGGACGCTTTCGCCCT
GCGCAGTCAGCAACGTACTGCCGCTGCCCAGGCTGCCGGCTACTTCGCCGAGGAAATCGTCGAAGTGCGGATCGCCCACA
AGAAGGGCGAAACCGTGGTCAGCCAGGACGAACACCCTCGTGCCGACACCACTATTGAAGCCCTGACCAAACTGAAACCG
GTCAACGGCCCGGAAAAGACCGTCACCGCCGGCAACGCGTCGGGCGTCAACGACGGTGCCGCCGCGCTGATTCTGGCCTC
GGCGGAAGCGGTGAAAAAACACGGTTTGACTGCCCGCGCCAAAGTGCTGGGCATGGCCAGCGCCGGGGTCGCGCCACGGG
TGATGGGCATCGGCCCGGTGCCGGCGGTGCGCAAACTCACCGAACGCCTCGGCGTTGCGGTCAGCGATTTCGATGTGATC
GAACTCAACGAAGCGTTTGCCAGCCAGGGTCTGGCGGTATTACGCGAGTTGGGGCTGGCGGACGACGCGGCCCAGGTCAA
CCCGAACGGTGGCGCGATTGCCTTGGGCCACCCGTTGGGCATGAGCGGTGCGCGACTGGTGCTGACCGCGCTGCATCAGC
TGGAAAAATCCGGTGGCAAGAAAGGTCTGGCGACCATGTGCGTCGGTGTCGGCCAGGGTCTGGCCCTTGCCATCGAACGC
GTCTGA

Upstream 100 bases:

>100_bases
ACGTTGACACCACCGCCGAACCGACCGAAGTCGAGCTGACCGCCCTGCGTGATCTCGAAGCCCGCACCGCAGCGGCCCAC
GGCCAGGCACCGGGAGAAGC

Downstream 100 bases:

>100_bases
CGCAAGCCGTGACGAAAAAGAACAGAGGAAAGCGAAATGACTGACAAGCCTGGTTACCGCCGCCCGCAGGAAGGCACCCA
GCCGCCGTACCTGCACCCGA

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MMRDVYICDAIRTPIGRFGGGLAAVRADDLAAVPIKALMERNPSVDWSAIDEVFLGCANQAGEDNRNVARMALLLAGLPE
SVPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVESMSRAPFVMGKADAAFSRNMKLEDTTIGWRFINPLMKAQYG
VDAMPQTADNVADDYEISREDQDAFALRSQQRTAAAQAAGYFAEEIVEVRIAHKKGETVVSQDEHPRADTTIEALTKLKP
VNGPEKTVTAGNASGVNDGAAALILASAEAVKKHGLTARAKVLGMASAGVAPRVMGIGPVPAVRKLTERLGVAVSDFDVI
ELNEAFASQGLAVLRELGLADDAAQVNPNGGAIALGHPLGMSGARLVLTALHQLEKSGGKKGLATMCVGVGQGLALAIER
V

Sequences:

>Translated_401_residues
MMRDVYICDAIRTPIGRFGGGLAAVRADDLAAVPIKALMERNPSVDWSAIDEVFLGCANQAGEDNRNVARMALLLAGLPE
SVPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVESMSRAPFVMGKADAAFSRNMKLEDTTIGWRFINPLMKAQYG
VDAMPQTADNVADDYEISREDQDAFALRSQQRTAAAQAAGYFAEEIVEVRIAHKKGETVVSQDEHPRADTTIEALTKLKP
VNGPEKTVTAGNASGVNDGAAALILASAEAVKKHGLTARAKVLGMASAGVAPRVMGIGPVPAVRKLTERLGVAVSDFDVI
ELNEAFASQGLAVLRELGLADDAAQVNPNGGAIALGHPLGMSGARLVLTALHQLEKSGGKKGLATMCVGVGQGLALAIER
V
>Mature_401_residues
MMRDVYICDAIRTPIGRFGGGLAAVRADDLAAVPIKALMERNPSVDWSAIDEVFLGCANQAGEDNRNVARMALLLAGLPE
SVPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVESMSRAPFVMGKADAAFSRNMKLEDTTIGWRFINPLMKAQYG
VDAMPQTADNVADDYEISREDQDAFALRSQQRTAAAQAAGYFAEEIVEVRIAHKKGETVVSQDEHPRADTTIEALTKLKP
VNGPEKTVTAGNASGVNDGAAALILASAEAVKKHGLTARAKVLGMASAGVAPRVMGIGPVPAVRKLTERLGVAVSDFDVI
ELNEAFASQGLAVLRELGLADDAAQVNPNGGAIALGHPLGMSGARLVLTALHQLEKSGGKKGLATMCVGVGQGLALAIER
V

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=401, Percent_Identity=42.3940149625935, Blast_Score=305, Evalue=4e-83,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=41.2060301507538, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=37.9652605459057, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI4501853, Length=403, Percent_Identity=38.9578163771712, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=30.6791569086651, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI194353979, Length=403, Percent_Identity=30.5210918114144, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=65.3366583541147, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1788554, Length=402, Percent_Identity=46.7661691542289, Blast_Score=310, Evalue=8e-86,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=45.1371571072319, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI48994986, Length=407, Percent_Identity=42.5061425061425, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=29.7560975609756, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI133906874, Length=399, Percent_Identity=41.6040100250627, Blast_Score=290, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI25147385, Length=405, Percent_Identity=37.7777777777778, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI17535921, Length=402, Percent_Identity=35.8208955223881, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17535917, Length=403, Percent_Identity=33.9950372208437, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17551802, Length=428, Percent_Identity=30.1401869158879, Blast_Score=161, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6325229, Length=408, Percent_Identity=38.2352941176471, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6322031, Length=408, Percent_Identity=35.2941176470588, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24655093, Length=400, Percent_Identity=45.75, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI17648125, Length=405, Percent_Identity=39.0123456790123, Blast_Score=254, Evalue=9e-68,
Organism=Drosophila melanogaster, GI24640423, Length=403, Percent_Identity=37.2208436724566, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=30.6791569086651, Blast_Score=168, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41797; Mature: 41797

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRDVYICDAIRTPIGRFGGGLAAVRADDLAAVPIKALMERNPSVDWSAIDEVFLGCANQ
CCCCEEEHHHHHCCHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
AGEDNRNVARMALLLAGLPESVPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVES
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCHHH
MSRAPFVMGKADAAFSRNMKLEDTTIGWRFINPLMKAQYGVDAMPQTADNVADDYEISRE
HHCCCEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCC
DQDAFALRSQQRTAAAQAAGYFAEEIVEVRIAHKKGETVVSQDEHPRADTTIEALTKLKP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCC
VNGPEKTVTAGNASGVNDGAAALILASAEAVKKHGLTARAKVLGMASAGVAPRVMGIGPV
CCCCCCEEECCCCCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCC
PAVRKLTERLGVAVSDFDVIELNEAFASQGLAVLRELGLADDAAQVNPNGGAIALGHPLG
HHHHHHHHHHCCEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCC
MSGARLVLTALHQLEKSGGKKGLATMCVGVGQGLALAIERV
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure
MMRDVYICDAIRTPIGRFGGGLAAVRADDLAAVPIKALMERNPSVDWSAIDEVFLGCANQ
CCCCEEEHHHHHCCHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
AGEDNRNVARMALLLAGLPESVPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVES
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCHHH
MSRAPFVMGKADAAFSRNMKLEDTTIGWRFINPLMKAQYGVDAMPQTADNVADDYEISRE
HHCCCEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCC
DQDAFALRSQQRTAAAQAAGYFAEEIVEVRIAHKKGETVVSQDEHPRADTTIEALTKLKP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCC
VNGPEKTVTAGNASGVNDGAAALILASAEAVKKHGLTARAKVLGMASAGVAPRVMGIGPV
CCCCCCEEECCCCCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCC
PAVRKLTERLGVAVSDFDVIELNEAFASQGLAVLRELGLADDAAQVNPNGGAIALGHPLG
HHHHHHHHHHCCEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCC
MSGARLVLTALHQLEKSGGKKGLATMCVGVGQGLALAIERV
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]