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The map label for this gene is cheB1

Identifier: 77457284

GI number: 77457284

Start: 1225571

End: 1226581

Strand: Direct

Name: cheB1

Synonym: Pfl01_1057

Alternate gene names: 77457284

Gene position: 1225571-1226581 (Clockwise)

Preceding gene: 77457283

Following gene: 77457285

Centisome position: 19.04

GC content: 64.59

Gene sequence:

>1011_bases
ATGAAGATCGCAATCGTCAATGACATGCCTCTGGCGGTGGAAGCCCTGCGTCGTGCGCTGGCCTTCGAGCCGGCGCATCA
GGTGGTGTGGGTCGCCAGCAACGGCGCCGAAGCGGTGCGCCTGTGCGCGGAAAACACCCCGGACCTGATCCTGATGGACC
TGATCATGCCGGTGATGGATGGCGTCGAGGCCACCCGCCGGATCATGGCCGAGAGCCCGTGCGCGATCGTCATCGTCACC
GTGGACCGCCAGCAGAACGTGCACCGGGTGTTCGAAGCCATGGGCCACGGCGCGCTGGACGTGGTCGACACCCCGGCCAT
CGGCGGTGGCAACCCTCAGGAAGCGGCGGCGCCACTGCTGCGTAAAATCCTTAATATCGGCTGGCTGATCGGCGAGAAAA
CCGCCCGCTCGCGCCCCGCACCTGCGGCACCGCGCAGTACCGCCTCGCGCCAGCGGCTGGTGGCGATCGGCTCTTCGGCC
GGTGGTCCGGCGGCGCTGGAAGTCTTGCTCAAGGGCCTGCCACGCCACTTTTCGGCGGCCATCGTGCTGGTGCAGCATGT
TGATCAGGTGTTCGCCGCCGGCATGGCCGAATGGCTGGCCAGCGCCAGCGGTCTGGATGTGCGTCTGGCCCGCGAAGGCG
AACCGCCGCAGGCCGGCGCGGTGTTGCTGGCCGGCACCAACCACCATATTCGTTTGCTCAAGAACGGCACGCTGGCCTAC
ACCGCCGAGCCGGTCAACGAGATCTACCGCCCCTCGATCGACGTGTTTTTCGAAAGCGTCGCCAACTACTGGAATGGTGA
CGCAGTAGGGGTTTTATTGACAGGGATGGGACGCGACGGGGCTCAAGGGCTTAAGCTCATGCGTCAACAGGGCTATCTGA
CCATCGCGCAGGATCAGCAAAGCAGTGCGGTGTACGGCATGCCCAAGGCTGCCGCAGCCATCGACGCGGCCGTAGAAATC
CGTCCACTGGAAAAGATAGCGCCACGATTGCTGGAGATATTCCCCAAATGA

Upstream 100 bases:

>100_bases
GTGGACTGGACGCCGGCGCCGACTATTATCTGGCCAAGGCCAGTTTTCATGACGACGCCCTGCTCGACGCGGTGGTTGAG
CTCATCGGAGGAGCGCGGGC

Downstream 100 bases:

>100_bases
ACGTGTTCCTCCGCATTCCTGGCCCCCGCAACACTCAGGTGACCGCCCATGAATGACTTACAGATCGATGACATCAAAAC
CGACGAGAACGCCGCCATGG

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKIAIVNDMPLAVEALRRALAFEPAHQVVWVASNGAEAVRLCAENTPDLILMDLIMPVMDGVEATRRIMAESPCAIVIVT
VDRQQNVHRVFEAMGHGALDVVDTPAIGGGNPQEAAAPLLRKILNIGWLIGEKTARSRPAPAAPRSTASRQRLVAIGSSA
GGPAALEVLLKGLPRHFSAAIVLVQHVDQVFAAGMAEWLASASGLDVRLAREGEPPQAGAVLLAGTNHHIRLLKNGTLAY
TAEPVNEIYRPSIDVFFESVANYWNGDAVGVLLTGMGRDGAQGLKLMRQQGYLTIAQDQQSSAVYGMPKAAAAIDAAVEI
RPLEKIAPRLLEIFPK

Sequences:

>Translated_336_residues
MKIAIVNDMPLAVEALRRALAFEPAHQVVWVASNGAEAVRLCAENTPDLILMDLIMPVMDGVEATRRIMAESPCAIVIVT
VDRQQNVHRVFEAMGHGALDVVDTPAIGGGNPQEAAAPLLRKILNIGWLIGEKTARSRPAPAAPRSTASRQRLVAIGSSA
GGPAALEVLLKGLPRHFSAAIVLVQHVDQVFAAGMAEWLASASGLDVRLAREGEPPQAGAVLLAGTNHHIRLLKNGTLAY
TAEPVNEIYRPSIDVFFESVANYWNGDAVGVLLTGMGRDGAQGLKLMRQQGYLTIAQDQQSSAVYGMPKAAAAIDAAVEI
RPLEKIAPRLLEIFPK
>Mature_336_residues
MKIAIVNDMPLAVEALRRALAFEPAHQVVWVASNGAEAVRLCAENTPDLILMDLIMPVMDGVEATRRIMAESPCAIVIVT
VDRQQNVHRVFEAMGHGALDVVDTPAIGGGNPQEAAAPLLRKILNIGWLIGEKTARSRPAPAAPRSTASRQRLVAIGSSA
GGPAALEVLLKGLPRHFSAAIVLVQHVDQVFAAGMAEWLASASGLDVRLAREGEPPQAGAVLLAGTNHHIRLLKNGTLAY
TAEPVNEIYRPSIDVFFESVANYWNGDAVGVLLTGMGRDGAQGLKLMRQQGYLTIAQDQQSSAVYGMPKAAAAIDAAVEI
RPLEKIAPRLLEIFPK

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=346, Percent_Identity=29.1907514450867, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_PSEPF (Q3KHF6)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346789.1
- ProteinModelPortal:   Q3KHF6
- SMR:   Q3KHF6
- STRING:   Q3KHF6
- GeneID:   3716206
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_1057
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   FFESVVE
- ProtClustDB:   PRK12555
- BioCyc:   PFLU205922:PFL_1057-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 35798; Mature: 35798

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 159-159 ACT_SITE 186-186 ACT_SITE 279-279

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIVNDMPLAVEALRRALAFEPAHQVVWVASNGAEAVRLCAENTPDLILMDLIMPVMD
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCEEHHHHHHHHHC
GVEATRRIMAESPCAIVIVTVDRQQNVHRVFEAMGHGALDVVDTPAIGGGNPQEAAAPLL
CHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHH
RKILNIGWLIGEKTARSRPAPAAPRSTASRQRLVAIGSSAGGPAALEVLLKGLPRHFSAA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHH
IVLVQHVDQVFAAGMAEWLASASGLDVRLAREGEPPQAGAVLLAGTNHHIRLLKNGTLAY
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEEECCCEEE
TAEPVNEIYRPSIDVFFESVANYWNGDAVGVLLTGMGRDGAQGLKLMRQQGYLTIAQDQQ
ECCHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCC
SSAVYGMPKAAAAIDAAVEIRPLEKIAPRLLEIFPK
CCCCCCCCHHHHHHHHHHEECCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIAIVNDMPLAVEALRRALAFEPAHQVVWVASNGAEAVRLCAENTPDLILMDLIMPVMD
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCEEHHHHHHHHHC
GVEATRRIMAESPCAIVIVTVDRQQNVHRVFEAMGHGALDVVDTPAIGGGNPQEAAAPLL
CHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHH
RKILNIGWLIGEKTARSRPAPAAPRSTASRQRLVAIGSSAGGPAALEVLLKGLPRHFSAA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHH
IVLVQHVDQVFAAGMAEWLASASGLDVRLAREGEPPQAGAVLLAGTNHHIRLLKNGTLAY
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEEECCCEEE
TAEPVNEIYRPSIDVFFESVANYWNGDAVGVLLTGMGRDGAQGLKLMRQQGYLTIAQDQQ
ECCHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCC
SSAVYGMPKAAAAIDAAVEIRPLEKIAPRLLEIFPK
CCCCCCCCHHHHHHHHHHEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA