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The map label for this gene is trmD [H]

Identifier: 77457248

GI number: 77457248

Start: 1185244

End: 1185996

Strand: Direct

Name: trmD [H]

Synonym: Pfl01_1021

Alternate gene names: 77457248

Gene position: 1185244-1185996 (Clockwise)

Preceding gene: 77457247

Following gene: 77457249

Centisome position: 18.41

GC content: 57.5

Gene sequence:

>753_bases
ATGGCCAGCCTGCGCGTAGACGTCATTACGCTGTTCCCCGAGATGTTCTCGGCCATCAGTGAGTACGGCATTACCAGTCG
TGCGGTCAAACAGGGGCTCTTGCAGCTGACCTGTTGGAATCCGCGGGATTACACGACGGATCGACATCACACTGTGGACG
ATCGCCCGTTTGGCGGTGGTCCGGGCATGGTGATGAAGATCAAGCCCCTGGAAGATGCTCTGGTTCAGGCCAAGGCAGCA
GCCGGGGAGGCGGCGAAGGTGATTTACCTGTCCCCCCAAGGCCGTCAACTGACTCAGTCGGCGGTACGCGAACTGGCGAA
ATCGGATGCATTGATCCTGATTGCCGGTCGCTATGAAGGCATTGACGAGCGCTTTATTGAGGCTCATGTCGATGAAGAGT
GGTCGATTGGTGACTATGTGTTGTCTGGCGGCGAGCTGCCGGCCATGGTCATGATCGATGCGGTTACACGACTGCTGCCC
GGAGCTTTAGGGCATGCGGATTCCGCTGAGGAAGATTCCTTTACGGATGGTCTGCTGGATTGCCCGCACTACACCCGACC
TGAGGTGTATGCGGATCAGCGTGTTCCCGACGTGTTGCTGAGTGGCAATCACGCGCATATCCGGCGTTGGCGTTTACAGC
AGTCCCTTGGTCGGACCTATGAACGACGCGCCGATCTTCTGGAAAGCCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTC
GAGGAATACATCCGCGAGCGGGACGATAGTTAA

Upstream 100 bases:

>100_bases
TGATCGCGAGCGGTTGTTGCCCTATACGGAGCAATGCGTGCTGGCAATCGACCTGGAAGCAGGCGTGATGAGGGTGGAAT
GGGACGCGGACTTCTAAGCA

Downstream 100 bases:

>100_bases
CAACGTATCGATGGTAGATCAGACGATTTACCTTAGGAGCACAGCATGACCAACAAAATCATCCTTGCACTCGAAGCAGA
GCAGATGACCAAAGAAATCC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MASLRVDVITLFPEMFSAISEYGITSRAVKQGLLQLTCWNPRDYTTDRHHTVDDRPFGGGPGMVMKIKPLEDALVQAKAA
AGEAAKVIYLSPQGRQLTQSAVRELAKSDALILIAGRYEGIDERFIEAHVDEEWSIGDYVLSGGELPAMVMIDAVTRLLP
GALGHADSAEEDSFTDGLLDCPHYTRPEVYADQRVPDVLLSGNHAHIRRWRLQQSLGRTYERRADLLESRSLSGEEKKLL
EEYIRERDDS

Sequences:

>Translated_250_residues
MASLRVDVITLFPEMFSAISEYGITSRAVKQGLLQLTCWNPRDYTTDRHHTVDDRPFGGGPGMVMKIKPLEDALVQAKAA
AGEAAKVIYLSPQGRQLTQSAVRELAKSDALILIAGRYEGIDERFIEAHVDEEWSIGDYVLSGGELPAMVMIDAVTRLLP
GALGHADSAEEDSFTDGLLDCPHYTRPEVYADQRVPDVLLSGNHAHIRRWRLQQSLGRTYERRADLLESRSLSGEEKKLL
EEYIRERDDS
>Mature_249_residues
ASLRVDVITLFPEMFSAISEYGITSRAVKQGLLQLTCWNPRDYTTDRHHTVDDRPFGGGPGMVMKIKPLEDALVQAKAAA
GEAAKVIYLSPQGRQLTQSAVRELAKSDALILIAGRYEGIDERFIEAHVDEEWSIGDYVLSGGELPAMVMIDAVTRLLPG
ALGHADSAEEDSFTDGLLDCPHYTRPEVYADQRVPDVLLSGNHAHIRRWRLQQSLGRTYERRADLLESRSLSGEEKKLLE
EYIRERDDS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=66.804979253112, Blast_Score=325, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27941; Mature: 27810

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLRVDVITLFPEMFSAISEYGITSRAVKQGLLQLTCWNPRDYTTDRHHTVDDRPFGGG
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCC
PGMVMKIKPLEDALVQAKAAAGEAAKVIYLSPQGRQLTQSAVRELAKSDALILIAGRYEG
CCEEEEEECHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCC
IDERFIEAHVDEEWSIGDYVLSGGELPAMVMIDAVTRLLPGALGHADSAEEDSFTDGLLD
HHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCHHCCCCCCCCCCCCCCCCCC
CPHYTRPEVYADQRVPDVLLSGNHAHIRRWRLQQSLGRTYERRADLLESRSLSGEEKKLL
CCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
EEYIRERDDS
HHHHHHCCCC
>Mature Secondary Structure 
ASLRVDVITLFPEMFSAISEYGITSRAVKQGLLQLTCWNPRDYTTDRHHTVDDRPFGGG
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCC
PGMVMKIKPLEDALVQAKAAAGEAAKVIYLSPQGRQLTQSAVRELAKSDALILIAGRYEG
CCEEEEEECHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCC
IDERFIEAHVDEEWSIGDYVLSGGELPAMVMIDAVTRLLPGALGHADSAEEDSFTDGLLD
HHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCHHCCCCCCCCCCCCCCCCCC
CPHYTRPEVYADQRVPDVLLSGNHAHIRRWRLQQSLGRTYERRADLLESRSLSGEEKKLL
CCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
EEYIRERDDS
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA