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The map label for this gene is gcvA [H]

Identifier: 77457148

GI number: 77457148

Start: 1071542

End: 1072489

Strand: Direct

Name: gcvA [H]

Synonym: Pfl01_0921

Alternate gene names: 77457148

Gene position: 1071542-1072489 (Clockwise)

Preceding gene: 77457144

Following gene: 77457149

Centisome position: 16.64

GC content: 62.87

Gene sequence:

>948_bases
ATGAGTCGTCAATTGCATGCCCAGACTTACGTCTGGCTGCAGGTGTTTTCCTGTGCCGCGCGGCACCTGTCATTCACCCG
TTGTGCCGAAGAACTGCACATCACGCCGGGGGCGGTGAGTCAGCAAATCCGACAGCTGGAAGAGCGACTGGGGTTTCGCC
TGTTTCATCGCCGTGCGCGAGGCGTGGAGTTGAGCGCGGAAGGGCAGCGACTGGCGATCACGGTCAACGAGGCTTACGGC
AGCATCGACGCGGAATTGCGTCGGCTGGATGCGGGGATGATCAGCGGGATTCTGCGGGTGCGCTCGATTCCATCGTTCCT
CAGCAAATGGCTGACCCCGCGCCTGCCGCGCCTGCAACAGCGTTACCCGGACATCCAGCTGCGGCTGGTGGCCGAGGACA
GCAGCGTGCCGTTGCATGAGGGGGATTTCGACCTGGCGATCGACCTGAACGACGGCAGTTATCCGGGACTGTTATCCACA
GCCTTGCTCGACGAGCAGATATTTCCGGTGTGCGCACCGGGCCTGTTGCGCGGACGGCCGCCGCTGCACGGGCCGGCGGA
TCTGGTGCATTTCCCGCTGTTGCACGACATCACCGCATGGCGCGGCAGTTATGAATACGCGGAGTGGGAGTTTTATCTGA
ATGCCATCGGTTTCGAGGGCGCGGATGTGCGGCGCGGGCACACGTTCAATCGCAACCACCTGACCATCGAAGCGGCGATT
GCCGGCATGGGCGTGGCGATTGCCCGGCGCACGCTGCTCAACGACGAGCTGGAGCGAGGGACGTTGATCGTGCCGTTCGG
GATTTCGGTGCCCAATCACAAGCGCTATGTCTTGCTTTATGCGCCGGGGGCCCTGAGCCATCCGGGGGTGCGGGCGGTGC
ATGACTGGCTGGTGGAAGAGGCGGGGATTTTTCGCAGCCTGCACCCGTTGGGTGATGGCCAATTGTGA

Upstream 100 bases:

>100_bases
GCCATTTTTTGTAGGACAAGCGCAATGTCGGCGATACTGCCTACCTCGCTTCGACGTGACTAACGAAACTTCCTAAGTAA
GCCTTTAGCAGGACTAAACC

Downstream 100 bases:

>100_bases
GCAGATTTTTCGGGGTGGCGGGGCAACCCAACTCCCGACCTTACCAGCGCTTTACTCGGTTGTCCGGCTTTTTTTGCGAA
TGAATATTTATCTTTTTTCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSRQLHAQTYVWLQVFSCAARHLSFTRCAEELHITPGAVSQQIRQLEERLGFRLFHRRARGVELSAEGQRLAITVNEAYG
SIDAELRRLDAGMISGILRVRSIPSFLSKWLTPRLPRLQQRYPDIQLRLVAEDSSVPLHEGDFDLAIDLNDGSYPGLLST
ALLDEQIFPVCAPGLLRGRPPLHGPADLVHFPLLHDITAWRGSYEYAEWEFYLNAIGFEGADVRRGHTFNRNHLTIEAAI
AGMGVAIARRTLLNDELERGTLIVPFGISVPNHKRYVLLYAPGALSHPGVRAVHDWLVEEAGIFRSLHPLGDGQL

Sequences:

>Translated_315_residues
MSRQLHAQTYVWLQVFSCAARHLSFTRCAEELHITPGAVSQQIRQLEERLGFRLFHRRARGVELSAEGQRLAITVNEAYG
SIDAELRRLDAGMISGILRVRSIPSFLSKWLTPRLPRLQQRYPDIQLRLVAEDSSVPLHEGDFDLAIDLNDGSYPGLLST
ALLDEQIFPVCAPGLLRGRPPLHGPADLVHFPLLHDITAWRGSYEYAEWEFYLNAIGFEGADVRRGHTFNRNHLTIEAAI
AGMGVAIARRTLLNDELERGTLIVPFGISVPNHKRYVLLYAPGALSHPGVRAVHDWLVEEAGIFRSLHPLGDGQL
>Mature_314_residues
SRQLHAQTYVWLQVFSCAARHLSFTRCAEELHITPGAVSQQIRQLEERLGFRLFHRRARGVELSAEGQRLAITVNEAYGS
IDAELRRLDAGMISGILRVRSIPSFLSKWLTPRLPRLQQRYPDIQLRLVAEDSSVPLHEGDFDLAIDLNDGSYPGLLSTA
LLDEQIFPVCAPGLLRGRPPLHGPADLVHFPLLHDITAWRGSYEYAEWEFYLNAIGFEGADVRRGHTFNRNHLTIEAAIA
GMGVAIARRTLLNDELERGTLIVPFGISVPNHKRYVLLYAPGALSHPGVRAVHDWLVEEAGIFRSLHPLGDGQL

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=36.551724137931, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=34.9480968858132, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=28.3783783783784, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI157672245, Length=165, Percent_Identity=33.9393939393939, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693193, Length=264, Percent_Identity=28.030303030303, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788748, Length=170, Percent_Identity=30.5882352941176, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=24.2424242424242, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787128, Length=150, Percent_Identity=28.6666666666667, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081978, Length=255, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789440, Length=257, Percent_Identity=26.4591439688716, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1790262, Length=244, Percent_Identity=26.2295081967213, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787806, Length=252, Percent_Identity=26.1904761904762, Blast_Score=61, Evalue=8e-11,
Organism=Escherichia coli, GI1790208, Length=168, Percent_Identity=27.3809523809524, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35314; Mature: 35183

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50931 HTH_LYSR ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQLHAQTYVWLQVFSCAARHLSFTRCAEELHITPGAVSQQIRQLEERLGFRLFHRRAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GVELSAEGQRLAITVNEAYGSIDAELRRLDAGMISGILRVRSIPSFLSKWLTPRLPRLQQ
CCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
RYPDIQLRLVAEDSSVPLHEGDFDLAIDLNDGSYPGLLSTALLDEQIFPVCAPGLLRGRP
HCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCC
PLHGPADLVHFPLLHDITAWRGSYEYAEWEFYLNAIGFEGADVRRGHTFNRNHLTIEAAI
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEHH
AGMGVAIARRTLLNDELERGTLIVPFGISVPNHKRYVLLYAPGALSHPGVRAVHDWLVEE
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHH
AGIFRSLHPLGDGQL
HHHHHHCCCCCCCCC
>Mature Secondary Structure 
SRQLHAQTYVWLQVFSCAARHLSFTRCAEELHITPGAVSQQIRQLEERLGFRLFHRRAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GVELSAEGQRLAITVNEAYGSIDAELRRLDAGMISGILRVRSIPSFLSKWLTPRLPRLQQ
CCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
RYPDIQLRLVAEDSSVPLHEGDFDLAIDLNDGSYPGLLSTALLDEQIFPVCAPGLLRGRP
HCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCC
PLHGPADLVHFPLLHDITAWRGSYEYAEWEFYLNAIGFEGADVRRGHTFNRNHLTIEAAI
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEHH
AGMGVAIARRTLLNDELERGTLIVPFGISVPNHKRYVLLYAPGALSHPGVRAVHDWLVEE
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHH
AGIFRSLHPLGDGQL
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]