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The map label for this gene is sdaA [H]

Identifier: 77457147

GI number: 77457147

Start: 1070030

End: 1071406

Strand: Reverse

Name: sdaA [H]

Synonym: Pfl01_0920

Alternate gene names: 77457147

Gene position: 1071406-1070030 (Counterclockwise)

Preceding gene: 77457150

Following gene: 77457146

Centisome position: 16.64

GC content: 63.33

Gene sequence:

>1377_bases
ATGGCTATCAGTGTTTTCGATCTATTCAAAGTCGGCATCGGCCCGTCCAGTTCCCACACCGTCGGCCCGATGCGCGCCGC
CGCGACCTTCGCCCAGGCGCTGATCGACCAGCATTTGCTGAACGATGTGCAGCGGGTGGAAATCCGTTTGTACGGCTCGC
TTTCGGCCACCGGTGTCGGCCATGCCACTGACCGCGCGACGGTCATGGGTTTGATGGGCGAATGGCCGGACAGCATTGAT
CCGTCGACCATCGAGCCTCGTATCCAGCAACTGCGCGAGACCGGCCAACTGTTTCTGGTCGGTGAACGTTCAATTGCCTT
CAACTGGCAGCACGATCTCCTGCTGCTGGACGAGAGCCTGCCCTACCACCCGAACGCCATGTCGCTGACGGCCTTCGGTG
CATCCGGCCAACTGTTCGAGCAAACGTACTACTCCGTCGGCGGCGGTTTCATCATCGAAGCGGCCGAAGCCGAATCCGGC
GTAGCGCCGGCCGGCGACGTCGAGTTGCCTTACGAATTCTCCAGCGCCGTCGAACTGCTGGCGCTGTGCAAGCAACACAA
CCTGCGGGTGTCCGAACTGATGATGGCCAACGAACGGGCCTGGCGCAGCGACGCCGAGATCCGTCAGGGCCTGCTGCACA
TCTGGTCGGTGATGCGCGAATGCGTCGAGCAAGGCCTGCGTCACGAAGGCATCCTGCCTGGCGGTCTGAATGTTCCCCGC
CGCGCTGCGAAATTGCACCGTAGCCTGCTGGAAATCGGCAAGCCGAACGTCATCAGTTCGACCCTGTCGGCGATGGAGTG
GGTCAACCTGTTCGCCCTCGCCGTCAACGAAGAAAACGCTGCCGGCGGGCGCATGGTGACCGCGCCGACCAACGGCGCTG
CCGGGATCATTCCGGCCGTTCTGCACTACTACATGAAATTCAATCCGGACGCGTCCGACGACGACGTGGTCGCGTTCTTT
CTGGGCGCGGCAGCGGTCGGCATCCTGTGCAAGAAAAACGCCTCGATCTCCGGCGCCGAAGTCGGCTGCCAGGGCGAAGT
CGGCTCTGCCTGCGCCATGGCCGCTGCGGGTCTGGCCGATGTGCTGGGCGCTACGCCCGAGCAACTGGAAAACGCCGCCG
AAATCGGCCTGGAACACAACCTCGGCCTGACCTGCGACCCGGTCGGCGGCCTTGTGCAGGTGCCGTGCATCGAGCGCAAC
GCCATCGCTGCGGTGAAGGCGATCAACGCCACGCAAATGGCCCTGCGCGGCGACGGCAAACACTTCATTTCCCTGGACCG
GGTGATCCGCACCATGCGCGATACCGGCGCCGACATGCATGACAAATACAAAGAGACTTCACGGGGCGGCTTGGCCGTGA
GCTGGGTGGAGTGCTGA

Upstream 100 bases:

>100_bases
CGTTAGTCACGTCGAAGCGAGGTAGGCAGTATCGCCGACATTGCGCTTGTCCTACAAAAAATGGCAGCGATTCCTACTCC
ATTGCTTGTAAGGGTTTAGC

Downstream 100 bases:

>100_bases
GGAGCGCTCCCTGACCGCCCGTAACACGTGAGCCCGAGCAAGAATAATAACGAGGCAAAAACGATGACCGATGTACGCAC
ACCTGCTGCCGAAAATCCCG

Product: L-serine ammonia-lyase

Products: NA

Alternate protein names: SDH; L-serine deaminase; L-SD [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MAISVFDLFKVGIGPSSSHTVGPMRAAATFAQALIDQHLLNDVQRVEIRLYGSLSATGVGHATDRATVMGLMGEWPDSID
PSTIEPRIQQLRETGQLFLVGERSIAFNWQHDLLLLDESLPYHPNAMSLTAFGASGQLFEQTYYSVGGGFIIEAAEAESG
VAPAGDVELPYEFSSAVELLALCKQHNLRVSELMMANERAWRSDAEIRQGLLHIWSVMRECVEQGLRHEGILPGGLNVPR
RAAKLHRSLLEIGKPNVISSTLSAMEWVNLFALAVNEENAAGGRMVTAPTNGAAGIIPAVLHYYMKFNPDASDDDVVAFF
LGAAAVGILCKKNASISGAEVGCQGEVGSACAMAAAGLADVLGATPEQLENAAEIGLEHNLGLTCDPVGGLVQVPCIERN
AIAAVKAINATQMALRGDGKHFISLDRVIRTMRDTGADMHDKYKETSRGGLAVSWVEC

Sequences:

>Translated_458_residues
MAISVFDLFKVGIGPSSSHTVGPMRAAATFAQALIDQHLLNDVQRVEIRLYGSLSATGVGHATDRATVMGLMGEWPDSID
PSTIEPRIQQLRETGQLFLVGERSIAFNWQHDLLLLDESLPYHPNAMSLTAFGASGQLFEQTYYSVGGGFIIEAAEAESG
VAPAGDVELPYEFSSAVELLALCKQHNLRVSELMMANERAWRSDAEIRQGLLHIWSVMRECVEQGLRHEGILPGGLNVPR
RAAKLHRSLLEIGKPNVISSTLSAMEWVNLFALAVNEENAAGGRMVTAPTNGAAGIIPAVLHYYMKFNPDASDDDVVAFF
LGAAAVGILCKKNASISGAEVGCQGEVGSACAMAAAGLADVLGATPEQLENAAEIGLEHNLGLTCDPVGGLVQVPCIERN
AIAAVKAINATQMALRGDGKHFISLDRVIRTMRDTGADMHDKYKETSRGGLAVSWVEC
>Mature_457_residues
AISVFDLFKVGIGPSSSHTVGPMRAAATFAQALIDQHLLNDVQRVEIRLYGSLSATGVGHATDRATVMGLMGEWPDSIDP
STIEPRIQQLRETGQLFLVGERSIAFNWQHDLLLLDESLPYHPNAMSLTAFGASGQLFEQTYYSVGGGFIIEAAEAESGV
APAGDVELPYEFSSAVELLALCKQHNLRVSELMMANERAWRSDAEIRQGLLHIWSVMRECVEQGLRHEGILPGGLNVPRR
AAKLHRSLLEIGKPNVISSTLSAMEWVNLFALAVNEENAAGGRMVTAPTNGAAGIIPAVLHYYMKFNPDASDDDVVAFFL
GAAAVGILCKKNASISGAEVGCQGEVGSACAMAAAGLADVLGATPEQLENAAEIGLEHNLGLTCDPVGGLVQVPCIERNA
IAAVKAINATQMALRGDGKHFISLDRVIRTMRDTGADMHDKYKETSRGGLAVSWVEC

Specific function: Deaminates Also Threonine, Particularly When It Is Present In High Concentration. [C]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI1789161, Length=457, Percent_Identity=54.2669584245077, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1788116, Length=457, Percent_Identity=53.6105032822757, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI48994925, Length=455, Percent_Identity=53.1868131868132, Blast_Score=473, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 48949; Mature: 48818

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISVFDLFKVGIGPSSSHTVGPMRAAATFAQALIDQHLLNDVQRVEIRLYGSLSATGVG
CEEEHEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCC
HATDRATVMGLMGEWPDSIDPSTIEPRIQQLRETGQLFLVGERSIAFNWQHDLLLLDESL
CCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCEEEEECCCCEEEEECCC
PYHPNAMSLTAFGASGQLFEQTYYSVGGGFIIEAAEAESGVAPAGDVELPYEFSSAVELL
CCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
ALCKQHNLRVSELMMANERAWRSDAEIRQGLLHIWSVMRECVEQGLRHEGILPGGLNVPR
HHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
RAAKLHRSLLEIGKPNVISSTLSAMEWVNLFALAVNEENAAGGRMVTAPTNGAAGIIPAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCEEEECCCCCCCHHHHHH
LHYYMKFNPDASDDDVVAFFLGAAAVGILCKKNASISGAEVGCQGEVGSACAMAAAGLAD
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VLGATPEQLENAAEIGLEHNLGLTCDPVGGLVQVPCIERNAIAAVKAINATQMALRGDGK
HHCCCHHHHHHHHHCCCCCCCCEEECCCCCEEECCCCCCCHHHHHHHCCHHHHEEECCCC
HFISLDRVIRTMRDTGADMHDKYKETSRGGLAVSWVEC
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
AISVFDLFKVGIGPSSSHTVGPMRAAATFAQALIDQHLLNDVQRVEIRLYGSLSATGVG
EEEHEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCC
HATDRATVMGLMGEWPDSIDPSTIEPRIQQLRETGQLFLVGERSIAFNWQHDLLLLDESL
CCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCEEEEECCCCEEEEECCC
PYHPNAMSLTAFGASGQLFEQTYYSVGGGFIIEAAEAESGVAPAGDVELPYEFSSAVELL
CCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
ALCKQHNLRVSELMMANERAWRSDAEIRQGLLHIWSVMRECVEQGLRHEGILPGGLNVPR
HHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
RAAKLHRSLLEIGKPNVISSTLSAMEWVNLFALAVNEENAAGGRMVTAPTNGAAGIIPAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCEEEECCCCCCCHHHHHH
LHYYMKFNPDASDDDVVAFFLGAAAVGILCKKNASISGAEVGCQGEVGSACAMAAAGLAD
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VLGATPEQLENAAEIGLEHNLGLTCDPVGGLVQVPCIERNAIAAVKAINATQMALRGDGK
HHCCCHHHHHHHHHCCCCCCCCEEECCCCCEEECCCCCCCHHHHHHHCCHHHHEEECCCC
HFISLDRVIRTMRDTGADMHDKYKETSRGGLAVSWVEC
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]