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The map label for this gene is pys2 [H]

Identifier: 77457131

GI number: 77457131

Start: 1046963

End: 1049158

Strand: Reverse

Name: pys2 [H]

Synonym: Pfl01_0904

Alternate gene names: 77457131

Gene position: 1049158-1046963 (Counterclockwise)

Preceding gene: 77457136

Following gene: 77457125

Centisome position: 16.3

GC content: 56.1

Gene sequence:

>2196_bases
ATGCAAAGGCCTCCAGCATTGGAGCTGACTGAACCTGTCCACACGACAGCGGTAGCGCCTCCCGGCTACAACCCGTTACC
GGCAACTACATTTGATGTCAGCAGCAGCGGCGCCTTTCAAAGGCCGCCTGCCTTTGCGGGCAACGTACATATCGTGAAAG
GCACCGCTCAAGTGCTGGATCAGGAGTTTCAGGCCAGAGCCAGTCAACTTGCACAAACCCTCGAACATGAACTTGCCGCC
GCCAGACTCGAAGTTTCAACTCACCCTCTGCCGCCCGCCGAGGCAATCATTCGTGAACTCGGTGTTCGCAATACGCTGAT
GGCGCGCAAGACTGCCGAGTTTCATCGCCAGACCGCCTTGGGCAACACGTTTTATGGCAGCGATCCCCTGAACCGGCCGT
TGAACGATTTCACCGCGCGAGCGCGAATGATGGAGCGCCCTCTCAAGCCTGATGGCATTGCCTTTCAGACCTGGTCGTTC
TCATACCGGGCCGCTCACGAAGCGAGGCTGTTGTCGCAATCGCTGCAGTTGCTCAACCAGCAGCAAGTCAATGTGCTCAA
CTGGCTGGCGACCGTTCAGGCCAACGATCAAGCGCGAATCGCCGCCGAGCAACAAGCGCAGAGAGCTGCTGCCGAACTGG
CGCGGATCAATGAACAAGCTCGACTTGAAGCGCTGGCACAAGCTGCACGTGTAGTGGCCGAGCGAGAGCGCGTTGCCGCC
GAACAACACCGTCAGCAAGCACTCGCAGAGGCGGCACGGCAGCAAAAGGAACAAGAGGAAATCCAGGCTCGTGAAGAGGC
GCGCCTTGCAGCGCTGGCTGAAGCACAACGGCTTGCTGCTGAGCAGGCGCAAATCGCCGCCGACGCTGCAGCGCAACAGA
TTGCTGCCGAACAGGCACGTCTGCAAGCGCAAGCCGATGCTCAGCGCCGCAATGAAGAGCGCTGGCTGCTGGCTGAAAAA
ACACGGCAGGAAAAACAGCAGAAAGCGCTGCGAAAGGCACAGAAAAAACGCCGTAAAAAAGCGCGCAAGATCGCCAGGGA
ACAGGCCCGACGGCAAGCAGAAAACGCCCTGAGCGGACAATCTCCCGTCTTTGCCAATGCCGGATCGATGGCTGCAATCG
GCCCGACCTTTACCGGGCCTCTCGGTGTTTTTGGCAACAACCCGGCAACCACGCTGGCACTCAGAACATCGCTGCGAACG
GCGGTGTCCCTGGCGCTGGCGGCCATATCCACAGCGGCGGCGCCGGTGATAGTCGGGTTCGCCGCTTTGCTCGTTCCCTC
GGAACTTGGGAACGGCGACCTGTTTGCTGCAAGTGTCCCGCTATCAGAACTTGCGCCTGATTTGGACACCGACCTGTACG
AACTGGCGACGACCGGCGGTGAAGTCGATCTGCCGGTCAGGCTTGGCTCCAGAACGTTCGGTAACACAGTCGAAGTTGTC
GTCGTCAGCACCGATGGCGTGACAGTCCCCAGCAAAGTGCCGGTGGTACTCGCACGCTTTGACGTACAGAAAAACACTTA
CATTTCCACTGCTAATGCAAGCGACTCCAAAGGCCCTGTCGTCACTTGGACGCCGTTGGTAAAACCGCTGAATCCTTCGA
CAGATTTGCCAGTCGCCGACACAGACTTGCCGAACTACGAAGGCGCCGATGTCACGCCTGACAGCGGACGGATCGATCCA
TTTCCTCAGCTTGATCAGTATGGGTTTGATGGGTGGGTTACGGTTTTCCCGATCGACTCTGGAATACCGCCGATTTTCAC
GATGTTTCGGGATCGGCGGCAGGATCCCGGTATCGCCAGTGGGATAGGGCAAACGGTTTCAGGCAATTGGTTGGGAACAG
CTGCGACGCAGCAAGGTGCGCCGATTCCGACGCAAATTGCGGATAAGCTCAGAGGTAGGGAGTTTTCGAGTTTCAGGGCA
TTTCGCAAGGCGTTTTGGAAGGCGGTAGGGGATGATCAAGCGTTGTTTGAACAATTCACCACTTTCAGTAAATTTGACAT
AAAAAAGGGTCTTTCACCAACAGCTCCCAGGTCAGAACAAGTGGGTGGAAAAACAAAACTGGATATACATCATGTCGTAC
CTATCGCTGAAGGTGGTTCGGTTTACGATCTAGACAATCTTCGTGTATTAACTCCAAAACGACACATTGAAACCCATTCC
GAAATGAGAAAACAAAAAAATGAAATTCAAGAATAA

Upstream 100 bases:

>100_bases
CTACAGGACTCTCGGACCCGCGCCCGGCGTTTCACCTGTAACTTTAAATGGCAGAACAACTCATCACTTTTAAACAACGC
ACATTCAAAAGGATATGAAT

Downstream 100 bases:

>100_bases
ATTTGAGGAATATACGGAAGCCGAATATGTAGCACTTATTAATCGGCTGGTCGAAGGTGATTACTCATCAGAAAAAGAGC
ATGACGCTATCGTCGACAAC

Product: S-type Pyocin

Products: NA

Alternate protein names: Killer protein [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MQRPPALELTEPVHTTAVAPPGYNPLPATTFDVSSSGAFQRPPAFAGNVHIVKGTAQVLDQEFQARASQLAQTLEHELAA
ARLEVSTHPLPPAEAIIRELGVRNTLMARKTAEFHRQTALGNTFYGSDPLNRPLNDFTARARMMERPLKPDGIAFQTWSF
SYRAAHEARLLSQSLQLLNQQQVNVLNWLATVQANDQARIAAEQQAQRAAAELARINEQARLEALAQAARVVAERERVAA
EQHRQQALAEAARQQKEQEEIQAREEARLAALAEAQRLAAEQAQIAADAAAQQIAAEQARLQAQADAQRRNEERWLLAEK
TRQEKQQKALRKAQKKRRKKARKIAREQARRQAENALSGQSPVFANAGSMAAIGPTFTGPLGVFGNNPATTLALRTSLRT
AVSLALAAISTAAAPVIVGFAALLVPSELGNGDLFAASVPLSELAPDLDTDLYELATTGGEVDLPVRLGSRTFGNTVEVV
VVSTDGVTVPSKVPVVLARFDVQKNTYISTANASDSKGPVVTWTPLVKPLNPSTDLPVADTDLPNYEGADVTPDSGRIDP
FPQLDQYGFDGWVTVFPIDSGIPPIFTMFRDRRQDPGIASGIGQTVSGNWLGTAATQQGAPIPTQIADKLRGREFSSFRA
FRKAFWKAVGDDQALFEQFTTFSKFDIKKGLSPTAPRSEQVGGKTKLDIHHVVPIAEGGSVYDLDNLRVLTPKRHIETHS
EMRKQKNEIQE

Sequences:

>Translated_731_residues
MQRPPALELTEPVHTTAVAPPGYNPLPATTFDVSSSGAFQRPPAFAGNVHIVKGTAQVLDQEFQARASQLAQTLEHELAA
ARLEVSTHPLPPAEAIIRELGVRNTLMARKTAEFHRQTALGNTFYGSDPLNRPLNDFTARARMMERPLKPDGIAFQTWSF
SYRAAHEARLLSQSLQLLNQQQVNVLNWLATVQANDQARIAAEQQAQRAAAELARINEQARLEALAQAARVVAERERVAA
EQHRQQALAEAARQQKEQEEIQAREEARLAALAEAQRLAAEQAQIAADAAAQQIAAEQARLQAQADAQRRNEERWLLAEK
TRQEKQQKALRKAQKKRRKKARKIAREQARRQAENALSGQSPVFANAGSMAAIGPTFTGPLGVFGNNPATTLALRTSLRT
AVSLALAAISTAAAPVIVGFAALLVPSELGNGDLFAASVPLSELAPDLDTDLYELATTGGEVDLPVRLGSRTFGNTVEVV
VVSTDGVTVPSKVPVVLARFDVQKNTYISTANASDSKGPVVTWTPLVKPLNPSTDLPVADTDLPNYEGADVTPDSGRIDP
FPQLDQYGFDGWVTVFPIDSGIPPIFTMFRDRRQDPGIASGIGQTVSGNWLGTAATQQGAPIPTQIADKLRGREFSSFRA
FRKAFWKAVGDDQALFEQFTTFSKFDIKKGLSPTAPRSEQVGGKTKLDIHHVVPIAEGGSVYDLDNLRVLTPKRHIETHS
EMRKQKNEIQE
>Mature_731_residues
MQRPPALELTEPVHTTAVAPPGYNPLPATTFDVSSSGAFQRPPAFAGNVHIVKGTAQVLDQEFQARASQLAQTLEHELAA
ARLEVSTHPLPPAEAIIRELGVRNTLMARKTAEFHRQTALGNTFYGSDPLNRPLNDFTARARMMERPLKPDGIAFQTWSF
SYRAAHEARLLSQSLQLLNQQQVNVLNWLATVQANDQARIAAEQQAQRAAAELARINEQARLEALAQAARVVAERERVAA
EQHRQQALAEAARQQKEQEEIQAREEARLAALAEAQRLAAEQAQIAADAAAQQIAAEQARLQAQADAQRRNEERWLLAEK
TRQEKQQKALRKAQKKRRKKARKIAREQARRQAENALSGQSPVFANAGSMAAIGPTFTGPLGVFGNNPATTLALRTSLRT
AVSLALAAISTAAAPVIVGFAALLVPSELGNGDLFAASVPLSELAPDLDTDLYELATTGGEVDLPVRLGSRTFGNTVEVV
VVSTDGVTVPSKVPVVLARFDVQKNTYISTANASDSKGPVVTWTPLVKPLNPSTDLPVADTDLPNYEGADVTPDSGRIDP
FPQLDQYGFDGWVTVFPIDSGIPPIFTMFRDRRQDPGIASGIGQTVSGNWLGTAATQQGAPIPTQIADKLRGREFSSFRA
FRKAFWKAVGDDQALFEQFTTFSKFDIKKGLSPTAPRSEQVGGKTKLDIHHVVPIAEGGSVYDLDNLRVLTPKRHIETHS
EMRKQKNEIQE

Specific function: Causes breakdown of chromosomal DNA as well as complete inhibition of lipid synthesis in sensitive cells [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the colicin/pyosin nuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR003060
- InterPro:   IPR010715
- InterPro:   IPR016128 [H]

Pfam domain/function: PF06958 Pyocin_S [H]

EC number: 3.1.21.1

Molecular weight: Translated: 79610; Mature: 79610

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRPPALELTEPVHTTAVAPPGYNPLPATTFDVSSSGAFQRPPAFAGNVHIVKGTAQVLD
CCCCCCCCCCCCCHHCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEECHHHHHH
QEFQARASQLAQTLEHELAAARLEVSTHPLPPAEAIIRELGVRNTLMARKTAEFHRQTAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHC
GNTFYGSDPLNRPLNDFTARARMMERPLKPDGIAFQTWSFSYRAAHEARLLSQSLQLLNQ
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
QQVNVLNWLATVQANDQARIAAEQQAQRAAAELARINEQARLEALAQAARVVAERERVAA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQHRQQALAEAARQQKEQEEIQAREEARLAALAEAQRLAAEQAQIAADAAAQQIAAEQAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQAQADAQRRNEERWLLAEKTRQEKQQKALRKAQKKRRKKARKIAREQARRQAENALSGQ
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPVFANAGSMAAIGPTFTGPLGVFGNNPATTLALRTSLRTAVSLALAAISTAAAPVIVGF
CCEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALLVPSELGNGDLFAASVPLSELAPDLDTDLYELATTGGEVDLPVRLGSRTFGNTVEVV
HHHHHHHCCCCCCEEEECCCHHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCEEEEE
VVSTDGVTVPSKVPVVLARFDVQKNTYISTANASDSKGPVVTWTPLVKPLNPSTDLPVAD
EEECCCEECCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCC
TDLPNYEGADVTPDSGRIDPFPQLDQYGFDGWVTVFPIDSGIPPIFTMFRDRRQDPGIAS
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCHHH
GIGQTVSGNWLGTAATQQGAPIPTQIADKLRGREFSSFRAFRKAFWKAVGDDQALFEQFT
HCCCCCCCCEEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
TFSKFDIKKGLSPTAPRSEQVGGKTKLDIHHVVPIAEGGSVYDLDNLRVLTPKRHIETHS
HHHHHHHHCCCCCCCCCCHHCCCCCEEEEEEEEEEECCCCEEECCCEEEECCHHHHHHHH
EMRKQKNEIQE
HHHHHHHHCCC
>Mature Secondary Structure
MQRPPALELTEPVHTTAVAPPGYNPLPATTFDVSSSGAFQRPPAFAGNVHIVKGTAQVLD
CCCCCCCCCCCCCHHCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEECHHHHHH
QEFQARASQLAQTLEHELAAARLEVSTHPLPPAEAIIRELGVRNTLMARKTAEFHRQTAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHC
GNTFYGSDPLNRPLNDFTARARMMERPLKPDGIAFQTWSFSYRAAHEARLLSQSLQLLNQ
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
QQVNVLNWLATVQANDQARIAAEQQAQRAAAELARINEQARLEALAQAARVVAERERVAA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQHRQQALAEAARQQKEQEEIQAREEARLAALAEAQRLAAEQAQIAADAAAQQIAAEQAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQAQADAQRRNEERWLLAEKTRQEKQQKALRKAQKKRRKKARKIAREQARRQAENALSGQ
HHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPVFANAGSMAAIGPTFTGPLGVFGNNPATTLALRTSLRTAVSLALAAISTAAAPVIVGF
CCEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALLVPSELGNGDLFAASVPLSELAPDLDTDLYELATTGGEVDLPVRLGSRTFGNTVEVV
HHHHHHHCCCCCCEEEECCCHHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCEEEEE
VVSTDGVTVPSKVPVVLARFDVQKNTYISTANASDSKGPVVTWTPLVKPLNPSTDLPVAD
EEECCCEECCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCC
TDLPNYEGADVTPDSGRIDPFPQLDQYGFDGWVTVFPIDSGIPPIFTMFRDRRQDPGIAS
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCHHH
GIGQTVSGNWLGTAATQQGAPIPTQIADKLRGREFSSFRAFRKAFWKAVGDDQALFEQFT
HCCCCCCCCEEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
TFSKFDIKKGLSPTAPRSEQVGGKTKLDIHHVVPIAEGGSVYDLDNLRVLTPKRHIETHS
HHHHHHHHCCCCCCCCCCHHCCCCCEEEEEEEEEEECCCCEEECCCEEEECCHHHHHHHH
EMRKQKNEIQE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491711; 10984043 [H]