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The map label for this gene is gatA [H]

Identifier: 77457063

GI number: 77457063

Start: 984300

End: 985751

Strand: Reverse

Name: gatA [H]

Synonym: Pfl01_0836

Alternate gene names: 77457063

Gene position: 985751-984300 (Counterclockwise)

Preceding gene: 77457064

Following gene: 77457062

Centisome position: 15.31

GC content: 62.95

Gene sequence:

>1452_bases
ATGCATCAATTGACTCTGGCCGAGATCGCCCGCGGTCTCGCCGATAAAAAGTTTTCTTCCGAAGAGCTGACCAAAGTCCT
GCTGGCGCGTATTACTCAGCTCGACCCGCAGCTCAACAGTTTCATCAGCCTCACCGAAGAGCTGGCCCTTGAGCAGGCGA
AAGCCGCCGACGTGCGCCGGGCCAATGGTGAGAGTGGCGCCCTGCTCGGTGCGCCGATCGCCCACAAGGATCTGTTCTGC
ACCCAGGGCATCCGCACCAGCTGCGGCTCGAAGATGCTCGACAACTTCAAGGCTCCATACGACGCCACCGTGGTGTCGAA
GCTGGCCGCCGCCGGCACCGTGACCCTGGGCAAGACCAACATGGACGAATTCGCCATGGGTTCGGCCAACGAGTCGAGCT
GGTACGGCGCGGTGAAAAACCCGTGGAACCTGGAACACGTGCCGGGCGGTTCGTCCGGTGGTTCGGCTGCCGCGGTTGCC
GCTCGTCTGTTGCCTGCCGCAACGGCCACCGACACCGGCGGTTCGATCCGTCAGCCAGCAGCATTCACCAACCTCACCGG
CCTGAAACCGACCTACGGTCGCGTGTCGCGCTGGGGCATGATCGCTTACGCCTCCAGCCTCGATCAGGGCGGCCCGCTGG
CTCGCACCGCCGAAGACTGCGCGATTTTGTTGCAAGGTATGGCCGGCTTCGATCCGAACGACTCCACCAGCATCGATGAG
CCAGTGCCGGACTACGCCGCCGGCCTGAATGGTTCGCTGCAAGGACTGCGTATCGGCGTGCCGAAGGAATACTTCGGTGC
CGGTCTCGACCCGCGCATCGCCGACCTGATCCAGAACAGCATCAAGGAGCTGCAGAAGCTCGGTGCTGTGATCAAGGAAA
TCAGCCTGCCGAACATGCAGCACGCGATCCCTGCGTACTACGTGATCGCCCCGGCAGAAGCCTCCTCCAACCTGTCGCGT
TTCGACGGCGTGCGCTTCGGCCACCGCTGCGCAGACCCGAAGAACCTGGAAGACCTGTACAAGCGCTCCCGTGGCGAAGG
CTTCGGGCCTGAAGTGCAGCGCCGGATCATGGTCGGTGCCTACGCGCTGTCCGCCGGTTACTACGACGCCTACTACCTGA
AAGCGCAGAAGATCCGTCGCCTGGTGAAGAACGACTTCATGGCTGCCTTTAATGAAGTCGACATCATCCTCGGCCCGACC
ACGCCAAACCCGGCCTGGAAACTCGGTGCCAAGAACAGCGACCCGGTCGCCGCCTACCTGGAAGACGTCTACACCATCAC
CGCCAACCTCGCGGGCCTGCCGGGCCTGTCGATGCCGGCCGGTTTCGTCGACGGCCTGCCGGTCGGCGTGCAACTGCTCG
CTCCGTATTTCCAGGAAGGCCGTTTGCTCAATGTTGCGCACCAGTATCAGTTGAACACTGACTGGCACACCCGCACCCCA
ACCGGCTTCTGA

Upstream 100 bases:

>100_bases
GAAAGCAATAACCGCGAGGCTTATCAGTCCATCGCACCAGCGGTCGAAAACGGCCTGTACCTGGTTCCGAAAGTCATCGA
CTAAAGGGAAAGAGCCTGCA

Downstream 100 bases:

>100_bases
GGAGACACACATGCAATGGGAAGTCGTGATCGGGCTGGAGATTCACACCCAGCTCACCACCCGGTCGAAAATCTTTTCCG
GTAGTTCCACCCAGTTCGGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MHQLTLAEIARGLADKKFSSEELTKVLLARITQLDPQLNSFISLTEELALEQAKAADVRRANGESGALLGAPIAHKDLFC
TQGIRTSCGSKMLDNFKAPYDATVVSKLAAAGTVTLGKTNMDEFAMGSANESSWYGAVKNPWNLEHVPGGSSGGSAAAVA
ARLLPAATATDTGGSIRQPAAFTNLTGLKPTYGRVSRWGMIAYASSLDQGGPLARTAEDCAILLQGMAGFDPNDSTSIDE
PVPDYAAGLNGSLQGLRIGVPKEYFGAGLDPRIADLIQNSIKELQKLGAVIKEISLPNMQHAIPAYYVIAPAEASSNLSR
FDGVRFGHRCADPKNLEDLYKRSRGEGFGPEVQRRIMVGAYALSAGYYDAYYLKAQKIRRLVKNDFMAAFNEVDIILGPT
TPNPAWKLGAKNSDPVAAYLEDVYTITANLAGLPGLSMPAGFVDGLPVGVQLLAPYFQEGRLLNVAHQYQLNTDWHTRTP
TGF

Sequences:

>Translated_483_residues
MHQLTLAEIARGLADKKFSSEELTKVLLARITQLDPQLNSFISLTEELALEQAKAADVRRANGESGALLGAPIAHKDLFC
TQGIRTSCGSKMLDNFKAPYDATVVSKLAAAGTVTLGKTNMDEFAMGSANESSWYGAVKNPWNLEHVPGGSSGGSAAAVA
ARLLPAATATDTGGSIRQPAAFTNLTGLKPTYGRVSRWGMIAYASSLDQGGPLARTAEDCAILLQGMAGFDPNDSTSIDE
PVPDYAAGLNGSLQGLRIGVPKEYFGAGLDPRIADLIQNSIKELQKLGAVIKEISLPNMQHAIPAYYVIAPAEASSNLSR
FDGVRFGHRCADPKNLEDLYKRSRGEGFGPEVQRRIMVGAYALSAGYYDAYYLKAQKIRRLVKNDFMAAFNEVDIILGPT
TPNPAWKLGAKNSDPVAAYLEDVYTITANLAGLPGLSMPAGFVDGLPVGVQLLAPYFQEGRLLNVAHQYQLNTDWHTRTP
TGF
>Mature_483_residues
MHQLTLAEIARGLADKKFSSEELTKVLLARITQLDPQLNSFISLTEELALEQAKAADVRRANGESGALLGAPIAHKDLFC
TQGIRTSCGSKMLDNFKAPYDATVVSKLAAAGTVTLGKTNMDEFAMGSANESSWYGAVKNPWNLEHVPGGSSGGSAAAVA
ARLLPAATATDTGGSIRQPAAFTNLTGLKPTYGRVSRWGMIAYASSLDQGGPLARTAEDCAILLQGMAGFDPNDSTSIDE
PVPDYAAGLNGSLQGLRIGVPKEYFGAGLDPRIADLIQNSIKELQKLGAVIKEISLPNMQHAIPAYYVIAPAEASSNLSR
FDGVRFGHRCADPKNLEDLYKRSRGEGFGPEVQRRIMVGAYALSAGYYDAYYLKAQKIRRLVKNDFMAAFNEVDIILGPT
TPNPAWKLGAKNSDPVAAYLEDVYTITANLAGLPGLSMPAGFVDGLPVGVQLLAPYFQEGRLLNVAHQYQLNTDWHTRTP
TGF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=493, Percent_Identity=38.3367139959432, Blast_Score=325, Evalue=7e-89,
Organism=Homo sapiens, GI195972892, Length=488, Percent_Identity=27.0491803278689, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI166795287, Length=210, Percent_Identity=27.1428571428571, Blast_Score=66, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=436, Percent_Identity=40.1376146788991, Blast_Score=291, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI17537465, Length=494, Percent_Identity=25.5060728744939, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=312, Percent_Identity=24.6794871794872, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=315, Percent_Identity=25.7142857142857, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=315, Percent_Identity=25.7142857142857, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=245, Percent_Identity=26.1224489795918, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=463, Percent_Identity=33.0453563714903, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6319685, Length=490, Percent_Identity=31.2244897959184, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320448, Length=529, Percent_Identity=24.007561436673, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648113, Length=482, Percent_Identity=40.4564315352697, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24644968, Length=508, Percent_Identity=25.9842519685039, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078093, Length=504, Percent_Identity=26.3888888888889, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24652985, Length=492, Percent_Identity=23.9837398373984, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922090, Length=492, Percent_Identity=23.9837398373984, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652981, Length=492, Percent_Identity=23.9837398373984, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652983, Length=492, Percent_Identity=23.9837398373984, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648435, Length=254, Percent_Identity=29.9212598425197, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648437, Length=254, Percent_Identity=29.9212598425197, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI45550774, Length=254, Percent_Identity=29.9212598425197, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21356731, Length=230, Percent_Identity=27.3913043478261, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648441, Length=185, Percent_Identity=31.8918918918919, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648439, Length=185, Percent_Identity=31.8918918918919, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51689; Mature: 51689

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQLTLAEIARGLADKKFSSEELTKVLLARITQLDPQLNSFISLTEELALEQAKAADVRR
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ANGESGALLGAPIAHKDLFCTQGIRTSCGSKMLDNFKAPYDATVVSKLAAAGTVTLGKTN
CCCCCCCEECCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCC
MDEFAMGSANESSWYGAVKNPWNLEHVPGGSSGGSAAAVAARLLPAATATDTGGSIRQPA
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
AFTNLTGLKPTYGRVSRWGMIAYASSLDQGGPLARTAEDCAILLQGMAGFDPNDSTSIDE
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
PVPDYAAGLNGSLQGLRIGVPKEYFGAGLDPRIADLIQNSIKELQKLGAVIKEISLPNMQ
CCCHHHHCCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
HAIPAYYVIAPAEASSNLSRFDGVRFGHRCADPKNLEDLYKRSRGEGFGPEVQRRIMVGA
HCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
YALSAGYYDAYYLKAQKIRRLVKNDFMAAFNEVDIILGPTTPNPAWKLGAKNSDPVAAYL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEECCCCCCCHHHHHH
EDVYTITANLAGLPGLSMPAGFVDGLPVGVQLLAPYFQEGRLLNVAHQYQLNTDWHTRTP
HHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCC
TGF
CCC
>Mature Secondary Structure
MHQLTLAEIARGLADKKFSSEELTKVLLARITQLDPQLNSFISLTEELALEQAKAADVRR
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ANGESGALLGAPIAHKDLFCTQGIRTSCGSKMLDNFKAPYDATVVSKLAAAGTVTLGKTN
CCCCCCCEECCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCC
MDEFAMGSANESSWYGAVKNPWNLEHVPGGSSGGSAAAVAARLLPAATATDTGGSIRQPA
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
AFTNLTGLKPTYGRVSRWGMIAYASSLDQGGPLARTAEDCAILLQGMAGFDPNDSTSIDE
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
PVPDYAAGLNGSLQGLRIGVPKEYFGAGLDPRIADLIQNSIKELQKLGAVIKEISLPNMQ
CCCHHHHCCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
HAIPAYYVIAPAEASSNLSRFDGVRFGHRCADPKNLEDLYKRSRGEGFGPEVQRRIMVGA
HCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
YALSAGYYDAYYLKAQKIRRLVKNDFMAAFNEVDIILGPTTPNPAWKLGAKNSDPVAAYL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEECCCCCCCHHHHHH
EDVYTITANLAGLPGLSMPAGFVDGLPVGVQLLAPYFQEGRLLNVAHQYQLNTDWHTRTP
HHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCC
TGF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA