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The map label for this gene is gatB [H]

Identifier: 77457062

GI number: 77457062

Start: 982844

End: 984289

Strand: Reverse

Name: gatB [H]

Synonym: Pfl01_0835

Alternate gene names: 77457062

Gene position: 984289-982844 (Counterclockwise)

Preceding gene: 77457063

Following gene: 77457061

Centisome position: 15.29

GC content: 61.0

Gene sequence:

>1446_bases
ATGCAATGGGAAGTCGTGATCGGGCTGGAGATTCACACCCAGCTCACCACCCGGTCGAAAATCTTTTCCGGTAGTTCCAC
CCAGTTCGGTTCCGAGCCGAACACCCAGGCCAGCCTGATCGACCTGGGCATGCCCGGCGTGCTGCCGGTGCTCAACCAGG
AAGCGGTGCGCATGGCGGTGATGTTCGGTCTGGCGATTGACGCCGAGATCGGTCAGCACAACGTGTTCGCCCGTAAAAAC
TACTTCTACCCGGACCTGCCGAAGGGCTACCAGATCAGCCAGATGGAGTTGCCGATCGTCGGCAAGGGCCACTTGGACAT
CGCTCTGGAAGACGGCACGGTCAAACGCGTCGGCATCACCCGCGCGCACCTGGAAGAAGACGCCGGCAAGAGCCTGCACG
AAGAATTCAGCGGTGCCACCGGCATCGACCTGAACCGTGCCGGCACGCCGCTGCTGGAAATCGTGTCCGAGCCTGACATG
CGCAGCGCCAAGGAAGCCGTGGCCTACGTCAAGGCGATCCACGCGCTGGTACGTTACCTGGGCATCTGCGATGGCAACAT
GGCCGAAGGCTCGCTGCGTTGCGACTGCAACGTGTCGATCCGTCCGAAAGGCCAGGCCGAGTTCGGCACCCGCTGTGAAA
TCAAGAACGTCAACTCGTTCCGCTTCATCGAGAAGGCGATCAACTCCGAGATCCAGCGTCAGATCGACCTGATCGAAGAC
GGCGGCAAAGTGATCCAGCAGACCCGTCTGTACGATCCGAACAAGGACGAAACCCGTCCGATGCGCAGCAAAGAAGAAGC
CAACGACTACCGTTACTTCCCCGATCCGGACCTGCTGCCGGTGGTCATCGAGGACTCGTTCCTCAATGACGTGCGCGCCA
CCCTGCCGGAACTGCCACCGCAGAAGCGCGAGCGCTTCCAGAGTGCGTTCGGTCTGTCGGCCTATGACGCCAACGTGCTG
GCCACCAGCCGTGAGCAGGCGGACTACTTCGAGAAAGTCGCCAGCATCGGCGGCGACGCCAAACTGGCGGCGAACTGGGT
GATGGTCGAGTTGGGCAGCCTGCTCAACAAGCAGAACCTGGACATCGAGGATTCGCCAGTTTCGGCCGAACAACTGGGCG
GCATGCTGCAGCGCATCAAGGACAACACCATCTCCGGCAAGATCGCCAAGGTGGTGTTTGAAGCGATGGCCAACGGCGAA
GGCAGCGCAGACGAGATCATCGAAAAGTGCGGCCTGAAGCAGGTTACCGACACCGGCGCAATCTCGGCGGTGCTGGACGA
AATGCTCGCGGCCAATGCCGAGCAGGTCGAGCAATACCGTGCGGCAGACGAAGCCAAGCGCGGCAAGATGTTCGGCTTCT
TCGTCGGCCAGGCCATGAAAGCCTCCAAAGGCAAGGCCAACCCGCAGCAAGTGAACGAACTGCTGAAAAGCAAGCTCGAA
GGCTGA

Upstream 100 bases:

>100_bases
CCGTATTTCCAGGAAGGCCGTTTGCTCAATGTTGCGCACCAGTATCAGTTGAACACTGACTGGCACACCCGCACCCCAAC
CGGCTTCTGAGGAGACACAC

Downstream 100 bases:

>100_bases
CCACAATGGAGCCAGATCCAAAGCCTGGCTCCATTCCCTGTGGGAGCGAGCATGCTCGCGACAGCGCAATTTCAGACACT
GCAAAAGTGACTGACGGATC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MQWEVVIGLEIHTQLTTRSKIFSGSSTQFGSEPNTQASLIDLGMPGVLPVLNQEAVRMAVMFGLAIDAEIGQHNVFARKN
YFYPDLPKGYQISQMELPIVGKGHLDIALEDGTVKRVGITRAHLEEDAGKSLHEEFSGATGIDLNRAGTPLLEIVSEPDM
RSAKEAVAYVKAIHALVRYLGICDGNMAEGSLRCDCNVSIRPKGQAEFGTRCEIKNVNSFRFIEKAINSEIQRQIDLIED
GGKVIQQTRLYDPNKDETRPMRSKEEANDYRYFPDPDLLPVVIEDSFLNDVRATLPELPPQKRERFQSAFGLSAYDANVL
ATSREQADYFEKVASIGGDAKLAANWVMVELGSLLNKQNLDIEDSPVSAEQLGGMLQRIKDNTISGKIAKVVFEAMANGE
GSADEIIEKCGLKQVTDTGAISAVLDEMLAANAEQVEQYRAADEAKRGKMFGFFVGQAMKASKGKANPQQVNELLKSKLE
G

Sequences:

>Translated_481_residues
MQWEVVIGLEIHTQLTTRSKIFSGSSTQFGSEPNTQASLIDLGMPGVLPVLNQEAVRMAVMFGLAIDAEIGQHNVFARKN
YFYPDLPKGYQISQMELPIVGKGHLDIALEDGTVKRVGITRAHLEEDAGKSLHEEFSGATGIDLNRAGTPLLEIVSEPDM
RSAKEAVAYVKAIHALVRYLGICDGNMAEGSLRCDCNVSIRPKGQAEFGTRCEIKNVNSFRFIEKAINSEIQRQIDLIED
GGKVIQQTRLYDPNKDETRPMRSKEEANDYRYFPDPDLLPVVIEDSFLNDVRATLPELPPQKRERFQSAFGLSAYDANVL
ATSREQADYFEKVASIGGDAKLAANWVMVELGSLLNKQNLDIEDSPVSAEQLGGMLQRIKDNTISGKIAKVVFEAMANGE
GSADEIIEKCGLKQVTDTGAISAVLDEMLAANAEQVEQYRAADEAKRGKMFGFFVGQAMKASKGKANPQQVNELLKSKLE
G
>Mature_481_residues
MQWEVVIGLEIHTQLTTRSKIFSGSSTQFGSEPNTQASLIDLGMPGVLPVLNQEAVRMAVMFGLAIDAEIGQHNVFARKN
YFYPDLPKGYQISQMELPIVGKGHLDIALEDGTVKRVGITRAHLEEDAGKSLHEEFSGATGIDLNRAGTPLLEIVSEPDM
RSAKEAVAYVKAIHALVRYLGICDGNMAEGSLRCDCNVSIRPKGQAEFGTRCEIKNVNSFRFIEKAINSEIQRQIDLIED
GGKVIQQTRLYDPNKDETRPMRSKEEANDYRYFPDPDLLPVVIEDSFLNDVRATLPELPPQKRERFQSAFGLSAYDANVL
ATSREQADYFEKVASIGGDAKLAANWVMVELGSLLNKQNLDIEDSPVSAEQLGGMLQRIKDNTISGKIAKVVFEAMANGE
GSADEIIEKCGLKQVTDTGAISAVLDEMLAANAEQVEQYRAADEAKRGKMFGFFVGQAMKASKGKANPQQVNELLKSKLE
G

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=499, Percent_Identity=37.2745490981964, Blast_Score=329, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI115534094, Length=299, Percent_Identity=39.7993311036789, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=512, Percent_Identity=31.640625, Blast_Score=230, Evalue=5e-61,
Organism=Drosophila melanogaster, GI45550808, Length=498, Percent_Identity=33.7349397590361, Blast_Score=286, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52853; Mature: 52853

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWEVVIGLEIHTQLTTRSKIFSGSSTQFGSEPNTQASLIDLGMPGVLPVLNQEAVRMAV
CCEEEEEEEEEEEHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
MFGLAIDAEIGQHNVFARKNYFYPDLPKGYQISQMELPIVGKGHLDIALEDGTVKRVGIT
HHHHHHCCCCCCCCEEEECCCCCCCCCCCCEECEEECCEEECCCEEEEECCCCEEEECCH
RAHLEEDAGKSLHEEFSGATGIDLNRAGTPLLEIVSEPDMRSAKEAVAYVKAIHALVRYL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GICDGNMAEGSLRCDCNVSIRPKGQAEFGTRCEIKNVNSFRFIEKAINSEIQRQIDLIED
HCCCCCCCCCCEEEEECEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
GGKVIQQTRLYDPNKDETRPMRSKEEANDYRYFPDPDLLPVVIEDSFLNDVRATLPELPP
CHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCH
QKRERFQSAFGLSAYDANVLATSREQADYFEKVASIGGDAKLAANWVMVELGSLLNKQNL
HHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
DIEDSPVSAEQLGGMLQRIKDNTISGKIAKVVFEAMANGEGSADEIIEKCGLKQVTDTGA
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHCCCHH
ISAVLDEMLAANAEQVEQYRAADEAKRGKMFGFFVGQAMKASKGKANPQQVNELLKSKLE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
G
C
>Mature Secondary Structure
MQWEVVIGLEIHTQLTTRSKIFSGSSTQFGSEPNTQASLIDLGMPGVLPVLNQEAVRMAV
CCEEEEEEEEEEEHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
MFGLAIDAEIGQHNVFARKNYFYPDLPKGYQISQMELPIVGKGHLDIALEDGTVKRVGIT
HHHHHHCCCCCCCCEEEECCCCCCCCCCCCEECEEECCEEECCCEEEEECCCCEEEECCH
RAHLEEDAGKSLHEEFSGATGIDLNRAGTPLLEIVSEPDMRSAKEAVAYVKAIHALVRYL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GICDGNMAEGSLRCDCNVSIRPKGQAEFGTRCEIKNVNSFRFIEKAINSEIQRQIDLIED
HCCCCCCCCCCEEEEECEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
GGKVIQQTRLYDPNKDETRPMRSKEEANDYRYFPDPDLLPVVIEDSFLNDVRATLPELPP
CHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCH
QKRERFQSAFGLSAYDANVLATSREQADYFEKVASIGGDAKLAANWVMVELGSLLNKQNL
HHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
DIEDSPVSAEQLGGMLQRIKDNTISGKIAKVVFEAMANGEGSADEIIEKCGLKQVTDTGA
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHCCCHH
ISAVLDEMLAANAEQVEQYRAADEAKRGKMFGFFVGQAMKASKGKANPQQVNELLKSKLE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA