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The map label for this gene is pleD [H]

Identifier: 77456851

GI number: 77456851

Start: 732894

End: 734564

Strand: Reverse

Name: pleD [H]

Synonym: Pfl01_0623

Alternate gene names: 77456851

Gene position: 734564-732894 (Counterclockwise)

Preceding gene: 77456858

Following gene: 77456850

Centisome position: 11.41

GC content: 62.66

Gene sequence:

>1671_bases
ATGACCGAGCCAGAAGACCCCAGCCGTGAGCGCCTCAAGCACCACTTTGCCCAGCGGGTAATTCATCAGGCACGTCAGAT
TCTTGAGATATGGCAGCGCCTGCAACGCAGTGAGTGGTCCACTGCCGATCTCGCCGAACTGAGCGAGGCCAATTTGCGCC
TGCTGCGTTTTGCCGAGCGTTTCGAACAGCCCGAACATACGCAGCTGGCCCGCCACATCAGCCAGTCGCTGGAAGCGGTG
GACGCCAATCGCGGACGCCTGAGCAGCGGCCTGATCACCGATCTCAACCGCCTGATGCAGCGCTTGTCGCGCACCGGCCT
GCGCCATGGCGATCAGCTTGATCAGACCTTCCTGCCGCCCCTGCGCAAACCGATCTACGTGATGCTGCAGGATCACGACC
GCGCCGAGCGGCTGGCCAAGCAGCTGGAGTTTTTCGGTCTGAGCGCCCAGGCGCTGGACAGCGTGTCGGCGTTTCGCTCG
TCGATGGTCGAGCGCCTGCCGGCCGCGATCGTCATGGACGTGGATTTCAGCGGCGCCGGCGTCGGCCTGAAACTCGCTGC
CGAAGCCCAGCAAGGTCTGGAAGAACCACTGCCGCTGCTGTTCTTCAGCCTGCACGAAACCGACACCCCGACCCGCCTAG
CGGCAGTGCGCGCCGGTGGCCAGGAATTCCTCACCGGCACCCTCGAAGCGTCGAGCCTGCTGGAGAAGATCGAAGTCCTG
ACCTGCGTCGCCCAGTACGAACCCTATAAAGTGCTGATCATCGACGACTCCCGCGCCCAGGCCCTGCACACCGAACGCCT
GCTCAACAGCGCCGGGATCGTCACCCGCACCCTGATCGAACCGATTCAAGCGATGGCCGAGCTGGCGGACTTCCAGCCAG
ACCTGATCATCCTCGACATGTACATGCCAGCCTGCACCGGCACCGAGCTGGCCAAGGTCATCCGCCACAACGACCGCTAC
GTCAGCGTGCCGATCATTTACCTGTCGGCCGAAGACGACCTGGACAAGCAGCTCGACGCCATGAGCGAAGGTGGCGACGA
CTTCCTGACCAAACCGATCAAGCCGCGCCACCTGATCACCACCGTGCGCAACCGCGCCGCCCGCGCGCGCAACCTGAAAG
CACGGATGGTCCGCGACAGCCTCACCGGCCTGTACAACCACACGCACATTCTGCAATTGCTCGAAGACTGCTCGTTCCGC
GCCCGCCGTGAAAACAAGCCGTTGAGCTTTGCCATGCTCGACATCGACCACTTCAAACGGGTCAATGATAGCCACGGCCA
CCCCATGGGCGACCGGGTCATCAAGAGCCTGGCGCTGTTCCTCAAGCAGCGTCTGCGCAAGACCGACTTCATCGGCCGTT
ACGGCGGTGAAGAATTCGCCATCGTCATGCCGGACACCGATATAGAAGCGGCGCACAAAGTGCTCGACGAAATCCGCCAG
CGCTTTGCCGAGATCCACTACCCGGCCCAGCCGCAGGATCTGTGGTGCACCTTCAGCGCCGGCGTGGTGGAGATGCACGA
CGACTCCGACAGCCTGATGATGGCCAGCCAGGCCGACGAGGCGCTGTATCGCGCCAAGGGCGAAGGACGCAATCGGGTGC
AGACCGCTCGCGACTCAAAGCAAAGTGCCACTTTTTCATCGGATTCGACCCAATCGGTCATAACCGTGTAA

Upstream 100 bases:

>100_bases
ACCGCAGATTATTGACGTACGACACTCATGTAAGGGAAAAGAGGTGCTAGCATAGAGCCACCAGTCGATCTCAGCATGCA
CCCTAACTAGTAGTCAGGAT

Downstream 100 bases:

>100_bases
CAGAACCGCAATAAATTCAGGCGCTTACCATTCTGCCGTTGGTAGCCGTCATGCGCCTGAAGTTGCTCACCAATCTCAAT
ACTCTGTTGCTGGTCGCCGT

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MTEPEDPSRERLKHHFAQRVIHQARQILEIWQRLQRSEWSTADLAELSEANLRLLRFAERFEQPEHTQLARHISQSLEAV
DANRGRLSSGLITDLNRLMQRLSRTGLRHGDQLDQTFLPPLRKPIYVMLQDHDRAERLAKQLEFFGLSAQALDSVSAFRS
SMVERLPAAIVMDVDFSGAGVGLKLAAEAQQGLEEPLPLLFFSLHETDTPTRLAAVRAGGQEFLTGTLEASSLLEKIEVL
TCVAQYEPYKVLIIDDSRAQALHTERLLNSAGIVTRTLIEPIQAMAELADFQPDLIILDMYMPACTGTELAKVIRHNDRY
VSVPIIYLSAEDDLDKQLDAMSEGGDDFLTKPIKPRHLITTVRNRAARARNLKARMVRDSLTGLYNHTHILQLLEDCSFR
ARRENKPLSFAMLDIDHFKRVNDSHGHPMGDRVIKSLALFLKQRLRKTDFIGRYGGEEFAIVMPDTDIEAAHKVLDEIRQ
RFAEIHYPAQPQDLWCTFSAGVVEMHDDSDSLMMASQADEALYRAKGEGRNRVQTARDSKQSATFSSDSTQSVITV

Sequences:

>Translated_556_residues
MTEPEDPSRERLKHHFAQRVIHQARQILEIWQRLQRSEWSTADLAELSEANLRLLRFAERFEQPEHTQLARHISQSLEAV
DANRGRLSSGLITDLNRLMQRLSRTGLRHGDQLDQTFLPPLRKPIYVMLQDHDRAERLAKQLEFFGLSAQALDSVSAFRS
SMVERLPAAIVMDVDFSGAGVGLKLAAEAQQGLEEPLPLLFFSLHETDTPTRLAAVRAGGQEFLTGTLEASSLLEKIEVL
TCVAQYEPYKVLIIDDSRAQALHTERLLNSAGIVTRTLIEPIQAMAELADFQPDLIILDMYMPACTGTELAKVIRHNDRY
VSVPIIYLSAEDDLDKQLDAMSEGGDDFLTKPIKPRHLITTVRNRAARARNLKARMVRDSLTGLYNHTHILQLLEDCSFR
ARRENKPLSFAMLDIDHFKRVNDSHGHPMGDRVIKSLALFLKQRLRKTDFIGRYGGEEFAIVMPDTDIEAAHKVLDEIRQ
RFAEIHYPAQPQDLWCTFSAGVVEMHDDSDSLMMASQADEALYRAKGEGRNRVQTARDSKQSATFSSDSTQSVITV
>Mature_555_residues
TEPEDPSRERLKHHFAQRVIHQARQILEIWQRLQRSEWSTADLAELSEANLRLLRFAERFEQPEHTQLARHISQSLEAVD
ANRGRLSSGLITDLNRLMQRLSRTGLRHGDQLDQTFLPPLRKPIYVMLQDHDRAERLAKQLEFFGLSAQALDSVSAFRSS
MVERLPAAIVMDVDFSGAGVGLKLAAEAQQGLEEPLPLLFFSLHETDTPTRLAAVRAGGQEFLTGTLEASSLLEKIEVLT
CVAQYEPYKVLIIDDSRAQALHTERLLNSAGIVTRTLIEPIQAMAELADFQPDLIILDMYMPACTGTELAKVIRHNDRYV
SVPIIYLSAEDDLDKQLDAMSEGGDDFLTKPIKPRHLITTVRNRAARARNLKARMVRDSLTGLYNHTHILQLLEDCSFRA
RRENKPLSFAMLDIDHFKRVNDSHGHPMGDRVIKSLALFLKQRLRKTDFIGRYGGEEFAIVMPDTDIEAAHKVLDEIRQR
FAEIHYPAQPQDLWCTFSAGVVEMHDDSDSLMMASQADEALYRAKGEGRNRVQTARDSKQSATFSSDSTQSVITV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=36.1581920903955, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=36.5269461077844, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=35.2201257861635, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1787802, Length=195, Percent_Identity=29.7435897435897, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=32.0987654320988, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=34.2857142857143, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1788381, Length=185, Percent_Identity=30.2702702702703, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87081974, Length=176, Percent_Identity=32.3863636363636, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=201, Percent_Identity=29.8507462686567, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788085, Length=160, Percent_Identity=29.375, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787541, Length=155, Percent_Identity=29.0322580645161, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081977, Length=166, Percent_Identity=31.9277108433735, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 62929; Mature: 62798

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPEDPSRERLKHHFAQRVIHQARQILEIWQRLQRSEWSTADLAELSEANLRLLRFAER
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FEQPEHTQLARHISQSLEAVDANRGRLSSGLITDLNRLMQRLSRTGLRHGDQLDQTFLPP
HCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCH
LRKPIYVMLQDHDRAERLAKQLEFFGLSAQALDSVSAFRSSMVERLPAAIVMDVDFSGAG
HCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
VGLKLAAEAQQGLEEPLPLLFFSLHETDTPTRLAAVRAGGQEFLTGTLEASSLLEKIEVL
CCCEEHHHHHHCCCCCHHHHHEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TCVAQYEPYKVLIIDDSRAQALHTERLLNSAGIVTRTLIEPIQAMAELADFQPDLIILDM
HHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YMPACTGTELAKVIRHNDRYVSVPIIYLSAEDDLDKQLDAMSEGGDDFLTKPIKPRHLIT
CCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCHHHCCCCCCHHHHH
TVRNRAARARNLKARMVRDSLTGLYNHTHILQLLEDCSFRARRENKPLSFAMLDIDHFKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHH
VNDSHGHPMGDRVIKSLALFLKQRLRKTDFIGRYGGEEFAIVMPDTDIEAAHKVLDEIRQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHH
RFAEIHYPAQPQDLWCTFSAGVVEMHDDSDSLMMASQADEALYRAKGEGRNRVQTARDSK
HHHHCCCCCCCCCEEEEECCCEEEEECCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHCCH
QSATFSSDSTQSVITV
HHCCCCCCCCCCEEEC
>Mature Secondary Structure 
TEPEDPSRERLKHHFAQRVIHQARQILEIWQRLQRSEWSTADLAELSEANLRLLRFAER
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FEQPEHTQLARHISQSLEAVDANRGRLSSGLITDLNRLMQRLSRTGLRHGDQLDQTFLPP
HCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCH
LRKPIYVMLQDHDRAERLAKQLEFFGLSAQALDSVSAFRSSMVERLPAAIVMDVDFSGAG
HCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
VGLKLAAEAQQGLEEPLPLLFFSLHETDTPTRLAAVRAGGQEFLTGTLEASSLLEKIEVL
CCCEEHHHHHHCCCCCHHHHHEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TCVAQYEPYKVLIIDDSRAQALHTERLLNSAGIVTRTLIEPIQAMAELADFQPDLIILDM
HHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YMPACTGTELAKVIRHNDRYVSVPIIYLSAEDDLDKQLDAMSEGGDDFLTKPIKPRHLIT
CCCCCCHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCHHHCCCCCCHHHHH
TVRNRAARARNLKARMVRDSLTGLYNHTHILQLLEDCSFRARRENKPLSFAMLDIDHFKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHH
VNDSHGHPMGDRVIKSLALFLKQRLRKTDFIGRYGGEEFAIVMPDTDIEAAHKVLDEIRQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHH
RFAEIHYPAQPQDLWCTFSAGVVEMHDDSDSLMMASQADEALYRAKGEGRNRVQTARDSK
HHHHCCCCCCCCCEEEEECCCEEEEECCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHCCH
QSATFSSDSTQSVITV
HHCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA