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The map label for this gene is rfaC [H]

Identifier: 77456694

GI number: 77456694

Start: 540475

End: 541536

Strand: Direct

Name: rfaC [H]

Synonym: Pfl01_0466

Alternate gene names: 77456694

Gene position: 540475-541536 (Clockwise)

Preceding gene: 77456693

Following gene: 77456695

Centisome position: 8.39

GC content: 63.09

Gene sequence:

>1062_bases
TTGCGGGTATTGCTGATCAAGACTTCATCGCTGGGCGACGTGATTCACGCATTGCCGGCGCTGACCGACGCCGCCCGGGC
GATTCCCGGGATCAGGTTCGACTGGGTGGTGGAAGAAGGCTTCGCCGAGATTCCGACCTGGCACCCGGCCGTAAGCAAAG
TGATTCCGGTGGCGATCCGCCGCTGGCGCAAAAATATCTGGAAAACCATCACCGGCGGCGAATGGAAGCGCTTCAAACAG
TCCGTTCGGGCGAACAAATATGACCTGGTGATCGACGCTCAGGGCTTGCTGAAAAGCGCCTGGCTGACCCGATACGTCAA
AGCCCCGGTGGCCGGTCTCGACAAGGGCTCGGCCCGCGAGCCGATCGCCGCGCGCTTTTATGACCGCAAACTGGCGGTGG
CCCGTGGGCAGCACGCCGTCGAGCGCGTGCGCCAGTTGTTCGCCATTGCTTTGGGTTACGACTTGCCCAAAGGCCTGGGC
GATTACGGCCTCAACGTCGAGCGTCTGGTCGAGCTGCCGCGCAAAAATGCTTACGTGGTGTTCCTGCATGGCACCACTTG
GGACACCAAACACTGGCCGGAAGCCTACTGGCGCGAGCTGACCGAACGGGTCGGCTACCTCGGCGTCGGGATCAAGTTGC
CGTGGGGCAACCCACTGGAGAAGGCCCGGGCCGAGCGCATCGCCGCCGGTTTCAAACACGCCGAAGTGCTGCCGAAACTG
AACCTGGCCGGGGTCGGCAAGGTGCTGGCCGGTGCCCAGGCCTGCGTCGCGGTGGACACCGGTCTTGGTCATCTGGCCGC
GGCACTCGATGTGCCGACGATTTCCCTGTTCGGCCCGACCAATCCCGGCCTGACCGGCGCCTACGGCAAGTTGCAGATTC
ACCTCGGCAGCGATTTCCCGTGCGCGCCGTGCCTGCAAAAGAAATGTACGTATCAACCGACCGCGCAGGATGCCGGTCAG
TTTGACCTGAAACGCGAGTGGCCTCTGTGCTTCACGCGTCTGAATCCCGAGCGTGTTGCCAGCCGACTGAGCACGTTGTT
GATGGCTGAGGAGCTGCGCTGA

Upstream 100 bases:

>100_bases
TGCCGTTTCGGCCATTACAACTGCCTGCGCCAGCTGATGCCGGACGCGGTCAACGATGCCTTGCAGCGTTTGCAGGGCTC
TGTGGTCGAGGTTCATTAAC

Downstream 100 bases:

>100_bases
TGCAATTGGCTTTTGTCCTGTACAAGTATTTCCCGTTCGGCGGTTTGCAGCGCGACTTCATGCGCATCGCCCTCGAATGC
CAGAAGCGCGGCCATCAAAT

Product: lipopolysaccharide heptosyltransferase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MRVLLIKTSSLGDVIHALPALTDAARAIPGIRFDWVVEEGFAEIPTWHPAVSKVIPVAIRRWRKNIWKTITGGEWKRFKQ
SVRANKYDLVIDAQGLLKSAWLTRYVKAPVAGLDKGSAREPIAARFYDRKLAVARGQHAVERVRQLFAIALGYDLPKGLG
DYGLNVERLVELPRKNAYVVFLHGTTWDTKHWPEAYWRELTERVGYLGVGIKLPWGNPLEKARAERIAAGFKHAEVLPKL
NLAGVGKVLAGAQACVAVDTGLGHLAAALDVPTISLFGPTNPGLTGAYGKLQIHLGSDFPCAPCLQKKCTYQPTAQDAGQ
FDLKREWPLCFTRLNPERVASRLSTLLMAEELR

Sequences:

>Translated_353_residues
MRVLLIKTSSLGDVIHALPALTDAARAIPGIRFDWVVEEGFAEIPTWHPAVSKVIPVAIRRWRKNIWKTITGGEWKRFKQ
SVRANKYDLVIDAQGLLKSAWLTRYVKAPVAGLDKGSAREPIAARFYDRKLAVARGQHAVERVRQLFAIALGYDLPKGLG
DYGLNVERLVELPRKNAYVVFLHGTTWDTKHWPEAYWRELTERVGYLGVGIKLPWGNPLEKARAERIAAGFKHAEVLPKL
NLAGVGKVLAGAQACVAVDTGLGHLAAALDVPTISLFGPTNPGLTGAYGKLQIHLGSDFPCAPCLQKKCTYQPTAQDAGQ
FDLKREWPLCFTRLNPERVASRLSTLLMAEELR
>Mature_353_residues
MRVLLIKTSSLGDVIHALPALTDAARAIPGIRFDWVVEEGFAEIPTWHPAVSKVIPVAIRRWRKNIWKTITGGEWKRFKQ
SVRANKYDLVIDAQGLLKSAWLTRYVKAPVAGLDKGSAREPIAARFYDRKLAVARGQHAVERVRQLFAIALGYDLPKGLG
DYGLNVERLVELPRKNAYVVFLHGTTWDTKHWPEAYWRELTERVGYLGVGIKLPWGNPLEKARAERIAAGFKHAEVLPKL
NLAGVGKVLAGAQACVAVDTGLGHLAAALDVPTISLFGPTNPGLTGAYGKLQIHLGSDFPCAPCLQKKCTYQPTAQDAGQ
FDLKREWPLCFTRLNPERVASRLSTLLMAEELR

Specific function: Heptose transfer to the lipopolysaccharide core. It transfers the innnermost heptose to [4'-P](3-deoxy-D-manno- octulosonic acid)2-IVA [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790051, Length=295, Percent_Identity=58.3050847457627, Blast_Score=345, Evalue=3e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011908 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 39113; Mature: 39113

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLIKTSSLGDVIHALPALTDAARAIPGIRFDWVVEEGFAEIPTWHPAVSKVIPVAIR
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCCHHHHHHHHHHHH
RWRKNIWKTITGGEWKRFKQSVRANKYDLVIDAQGLLKSAWLTRYVKAPVAGLDKGSARE
HHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PIAARFYDRKLAVARGQHAVERVRQLFAIALGYDLPKGLGDYGLNVERLVELPRKNAYVV
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEEEE
FLHGTTWDTKHWPEAYWRELTERVGYLGVGIKLPWGNPLEKARAERIAAGFKHAEVLPKL
EEECCCCCCCCCHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHCCCC
NLAGVGKVLAGAQACVAVDTGLGHLAAALDVPTISLFGPTNPGLTGAYGKLQIHLGSDFP
CHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEECCCCC
CAPCLQKKCTYQPTAQDAGQFDLKREWPLCFTRLNPERVASRLSTLLMAEELR
CCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVLLIKTSSLGDVIHALPALTDAARAIPGIRFDWVVEEGFAEIPTWHPAVSKVIPVAIR
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCCCCHHHHHHHHHHHH
RWRKNIWKTITGGEWKRFKQSVRANKYDLVIDAQGLLKSAWLTRYVKAPVAGLDKGSARE
HHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PIAARFYDRKLAVARGQHAVERVRQLFAIALGYDLPKGLGDYGLNVERLVELPRKNAYVV
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEEEE
FLHGTTWDTKHWPEAYWRELTERVGYLGVGIKLPWGNPLEKARAERIAAGFKHAEVLPKL
EEECCCCCCCCCHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHCCCC
NLAGVGKVLAGAQACVAVDTGLGHLAAALDVPTISLFGPTNPGLTGAYGKLQIHLGSDFP
CHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEECCCCC
CAPCLQKKCTYQPTAQDAGQFDLKREWPLCFTRLNPERVASRLSTLLMAEELR
CCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8478319; 8041620; 9278503; 1624462 [H]