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The map label for this gene is rfaF [H]

Identifier: 77456693

GI number: 77456693

Start: 539439

End: 540473

Strand: Direct

Name: rfaF [H]

Synonym: Pfl01_0465

Alternate gene names: 77456693

Gene position: 539439-540473 (Clockwise)

Preceding gene: 77456692

Following gene: 77456694

Centisome position: 8.38

GC content: 63.77

Gene sequence:

>1035_bases
ATGAATATTCTGATCGTTGGGCCCAGTTGGGTCGGTGACATGGTGATGGCGCAGACACTGTTTCAGTGTCTCAAGCAGCG
CCACCCGCAGTGCGAAATCGACGTGCTGGCCCCGGAGTGGAGCCGGCCGATCCTCGAACGCATGCCCGAAGTGCGCAAGG
CCTTGAGCTTCCCGCTCGGCCACGGCGCGCTGGAGCTGGCGACCCGTCGGCGGATCGGCAAATCCCTGGCCGGCCAGTAC
GACCAGGCGATCCTGCTGCCGAATTCGTTGAAGTCGGCACTGGTGCCGTTCTTCGCCGGCATTCCGAAACGCACCGGCTG
GCGCGGCGAATTCCGCTACGGCCTGCTCAATGACGTGCGCACGCTGGATAAAGAACGTTATCCGCTGATGATCGAGCGTT
TCATGGCGCTGGCGTTTGAACCGAACGCCGAACTGCCGAAACCCTATCCGCGCCCGAGCCTGCAAATCGACCCGGTGACC
CGCGAGGCCGCATTGGCCAAGTTCGGCCTGACCCTCGACCGTCCGGTGCTGGTGTTGTGCCCCGGCGCCGAATTCGGTGA
AGCCAAGCGCTGGCCGTCTGAGCATTATGCCAAGGTCGCCGAGGCGCGCATCCGTGAAGGCTGGCAGGTCTGGCTGTTCG
GTTCGAAGAACGATCACGCGGTCGGCGAAGACATCCGCGCGCGGCTGATTCCGGGTCTGCGTGAAGAGTCGGTGAATCTG
AGCGGCGGCACTTCGCTGGCCGAGGCCATCGACCTGATGTCCTGCGCCGATTCGGTCGTCTCCAACGATTCCGGCCTGAT
GCACGTGGCCGCTGCGCTCAATCGCCCGTTGGTGGCGGTCTACGGTTCGACCTCGCCGGGTTTCACCCCGCCGCTGGCCG
AACACGTGGAAATCGTCCGTCTGGGCCTCGATTGCAGCCCTTGCTTCGACCGCACCTGCCGTTTCGGCCATTACAACTGC
CTGCGCCAGCTGATGCCGGACGCGGTCAACGATGCCTTGCAGCGTTTGCAGGGCTCTGTGGTCGAGGTTCATTAA

Upstream 100 bases:

>100_bases
CGAACGGCGGCAGGTGATGCGGATCTGGCGTGAGCTGGGGCTAAGCTGATTCTCGAGACGGGGAGGCGTAAAGCCTCCCC
GGTTCGTTTATGGAAACCAC

Downstream 100 bases:

>100_bases
CTTGCGGGTATTGCTGATCAAGACTTCATCGCTGGGCGACGTGATTCACGCATTGCCGGCGCTGACCGACGCCGCCCGGG
CGATTCCCGGGATCAGGTTC

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNILIVGPSWVGDMVMAQTLFQCLKQRHPQCEIDVLAPEWSRPILERMPEVRKALSFPLGHGALELATRRRIGKSLAGQY
DQAILLPNSLKSALVPFFAGIPKRTGWRGEFRYGLLNDVRTLDKERYPLMIERFMALAFEPNAELPKPYPRPSLQIDPVT
REAALAKFGLTLDRPVLVLCPGAEFGEAKRWPSEHYAKVAEARIREGWQVWLFGSKNDHAVGEDIRARLIPGLREESVNL
SGGTSLAEAIDLMSCADSVVSNDSGLMHVAAALNRPLVAVYGSTSPGFTPPLAEHVEIVRLGLDCSPCFDRTCRFGHYNC
LRQLMPDAVNDALQRLQGSVVEVH

Sequences:

>Translated_344_residues
MNILIVGPSWVGDMVMAQTLFQCLKQRHPQCEIDVLAPEWSRPILERMPEVRKALSFPLGHGALELATRRRIGKSLAGQY
DQAILLPNSLKSALVPFFAGIPKRTGWRGEFRYGLLNDVRTLDKERYPLMIERFMALAFEPNAELPKPYPRPSLQIDPVT
REAALAKFGLTLDRPVLVLCPGAEFGEAKRWPSEHYAKVAEARIREGWQVWLFGSKNDHAVGEDIRARLIPGLREESVNL
SGGTSLAEAIDLMSCADSVVSNDSGLMHVAAALNRPLVAVYGSTSPGFTPPLAEHVEIVRLGLDCSPCFDRTCRFGHYNC
LRQLMPDAVNDALQRLQGSVVEVH
>Mature_344_residues
MNILIVGPSWVGDMVMAQTLFQCLKQRHPQCEIDVLAPEWSRPILERMPEVRKALSFPLGHGALELATRRRIGKSLAGQY
DQAILLPNSLKSALVPFFAGIPKRTGWRGEFRYGLLNDVRTLDKERYPLMIERFMALAFEPNAELPKPYPRPSLQIDPVT
REAALAKFGLTLDRPVLVLCPGAEFGEAKRWPSEHYAKVAEARIREGWQVWLFGSKNDHAVGEDIRARLIPGLREESVNL
SGGTSLAEAIDLMSCADSVVSNDSGLMHVAAALNRPLVAVYGSTSPGFTPPLAEHVEIVRLGLDCSPCFDRTCRFGHYNC
LRQLMPDAVNDALQRLQGSVVEVH

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=342, Percent_Identity=51.7543859649123, Blast_Score=355, Evalue=3e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 38233; Mature: 38233

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIVGPSWVGDMVMAQTLFQCLKQRHPQCEIDVLAPEWSRPILERMPEVRKALSFPLG
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
HGALELATRRRIGKSLAGQYDQAILLPNSLKSALVPFFAGIPKRTGWRGEFRYGLLNDVR
CCHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
TLDKERYPLMIERFMALAFEPNAELPKPYPRPSLQIDPVTREAALAKFGLTLDRPVLVLC
HHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCCCEEEEE
PGAEFGEAKRWPSEHYAKVAEARIREGWQVWLFGSKNDHAVGEDIRARLIPGLREESVNL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCCHHCCCCC
SGGTSLAEAIDLMSCADSVVSNDSGLMHVAAALNRPLVAVYGSTSPGFTPPLAEHVEIVR
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHH
LGLDCSPCFDRTCRFGHYNCLRQLMPDAVNDALQRLQGSVVEVH
CCCCCCHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCEECC
>Mature Secondary Structure
MNILIVGPSWVGDMVMAQTLFQCLKQRHPQCEIDVLAPEWSRPILERMPEVRKALSFPLG
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
HGALELATRRRIGKSLAGQYDQAILLPNSLKSALVPFFAGIPKRTGWRGEFRYGLLNDVR
CCHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
TLDKERYPLMIERFMALAFEPNAELPKPYPRPSLQIDPVTREAALAKFGLTLDRPVLVLC
HHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCCCEEEEE
PGAEFGEAKRWPSEHYAKVAEARIREGWQVWLFGSKNDHAVGEDIRARLIPGLREESVNL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCCHHCCCCC
SGGTSLAEAIDLMSCADSVVSNDSGLMHVAAALNRPLVAVYGSTSPGFTPPLAEHVEIVR
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHH
LGLDCSPCFDRTCRFGHYNCLRQLMPDAVNDALQRLQGSVVEVH
CCCCCCHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]