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The map label for this gene is lip2 [H]

Identifier: 77456598

GI number: 77456598

Start: 422929

End: 423846

Strand: Direct

Name: lip2 [H]

Synonym: Pfl01_0370

Alternate gene names: 77456598

Gene position: 422929-423846 (Clockwise)

Preceding gene: 77456597

Following gene: 77456599

Centisome position: 6.57

GC content: 66.88

Gene sequence:

>918_bases
ATGGGCACTTATCCACTCTCGCCGTCGATGGCGGCGTTCGTCGCCAGAACCGAAAGCTTCGCCAGCGATGACAGCAGCCT
CGGCGGATTGCGCAAATCCTATGACGACATGTGCCGCGCCTTCACGCCCGAGCGGCCGGCGGGTCTGGAGGTGGTGGATT
TCCAGTTGAGCGGGGTGGCGGTGCGCTCGTATCGCCCGCCTGTCCGGCCATCGGCCAGTGGCTGGCCGTGCGTGTTGTAT
CTGCATGGCGGCGGTTGGGTGGTCGGCGGGCTGGATTCCCATGACTTCATCTGCTGCGAGCTGGCCATGGCGCTCGGGGC
GATGGTGGTGGTGGTGGATTACCGGCTGGCGCCGGAGCACCCGTTCCCGGCCGGTTTCGAGGATTGCCTGAGCGTCTGGC
GCGCGTTGCGCAGCGGACCGTTCTGGTTCGATCCCGGACGGATGCTGGTGGCGGGCGACAGCGCCGGCGGCAATCTGGCG
GCGGCGCTGTGCCTGGCGCTACGCGATGCCGGCGAGCCGCTGCCGGGCGCGCAGGTCTTGATCTATCCGGGGCTGGGTGG
CGACGCACAGTTACCGTCGCGCAGCGAATGCGCCGATGCGCCGTTGCTCGCCAGCAGCGATGTCGATTGTTATCACGCCT
TGTACCTGCGCGGCACCGCGAAGCCGAACGCCTATGCGATGCCATTGCTGGCCGGGGACTTCAGCGGTTTGCCACCGGCC
TGGATCGCCGTGGCGCAGTTCGATCCGCTGCGTGACGACGGCGTGTGCTATGCCGAACGGCTTGACGCAGCGGGCGTCGA
CGCGGCGCTGTATTACGGCGAAGGGCTGGTTCACGGCTGCCTGCGGGCGCGACATCAGGTCGCCGAGGTCGATCGTCTCT
TTGAGAACCTGCTGGGGTTCATGGCTGACAAAATGTGA

Upstream 100 bases:

>100_bases
TGCGCGAGCATCCCGAAGACCTGCAACGCTGGCTGGCGGGTGTCAGCAGTTTCGATGGCAAGGACGGCGTGGCCACGGTT
CAGGCCAGCCTGAAAAACTG

Downstream 100 bases:

>100_bases
CAGCGCACGGGCATGCTCATTGACGTAAATCGGGTTTATGATGCCGAACGGCAGAATAATAGAAGTCCCCCCAGGGATGA
CCTCGACCCCTTACGGAGCG

Product: lipolytic protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MGTYPLSPSMAAFVARTESFASDDSSLGGLRKSYDDMCRAFTPERPAGLEVVDFQLSGVAVRSYRPPVRPSASGWPCVLY
LHGGGWVVGGLDSHDFICCELAMALGAMVVVVDYRLAPEHPFPAGFEDCLSVWRALRSGPFWFDPGRMLVAGDSAGGNLA
AALCLALRDAGEPLPGAQVLIYPGLGGDAQLPSRSECADAPLLASSDVDCYHALYLRGTAKPNAYAMPLLAGDFSGLPPA
WIAVAQFDPLRDDGVCYAERLDAAGVDAALYYGEGLVHGCLRARHQVAEVDRLFENLLGFMADKM

Sequences:

>Translated_305_residues
MGTYPLSPSMAAFVARTESFASDDSSLGGLRKSYDDMCRAFTPERPAGLEVVDFQLSGVAVRSYRPPVRPSASGWPCVLY
LHGGGWVVGGLDSHDFICCELAMALGAMVVVVDYRLAPEHPFPAGFEDCLSVWRALRSGPFWFDPGRMLVAGDSAGGNLA
AALCLALRDAGEPLPGAQVLIYPGLGGDAQLPSRSECADAPLLASSDVDCYHALYLRGTAKPNAYAMPLLAGDFSGLPPA
WIAVAQFDPLRDDGVCYAERLDAAGVDAALYYGEGLVHGCLRARHQVAEVDRLFENLLGFMADKM
>Mature_304_residues
GTYPLSPSMAAFVARTESFASDDSSLGGLRKSYDDMCRAFTPERPAGLEVVDFQLSGVAVRSYRPPVRPSASGWPCVLYL
HGGGWVVGGLDSHDFICCELAMALGAMVVVVDYRLAPEHPFPAGFEDCLSVWRALRSGPFWFDPGRMLVAGDSAGGNLAA
ALCLALRDAGEPLPGAQVLIYPGLGGDAQLPSRSECADAPLLASSDVDCYHALYLRGTAKPNAYAMPLLAGDFSGLPPAW
IAVAQFDPLRDDGVCYAERLDAAGVDAALYYGEGLVHGCLRARHQVAEVDRLFENLLGFMADKM

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=297, Percent_Identity=30.3030303030303, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI68299767, Length=335, Percent_Identity=28.955223880597, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI68051721, Length=182, Percent_Identity=33.5164835164835, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI157041239, Length=165, Percent_Identity=37.5757575757576, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI226423947, Length=190, Percent_Identity=32.1052631578947, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI61966717, Length=166, Percent_Identity=34.3373493975904, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI157041237, Length=125, Percent_Identity=38.4, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786682, Length=262, Percent_Identity=28.6259541984733, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17567059, Length=287, Percent_Identity=28.9198606271777, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI72001146, Length=330, Percent_Identity=26.0606060606061, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71996133, Length=187, Percent_Identity=30.4812834224599, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540028, Length=178, Percent_Identity=31.4606741573034, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24656084, Length=145, Percent_Identity=33.1034482758621, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24656076, Length=145, Percent_Identity=33.1034482758621, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20130169, Length=145, Percent_Identity=33.1034482758621, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 32499; Mature: 32368

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTYPLSPSMAAFVARTESFASDDSSLGGLRKSYDDMCRAFTPERPAGLEVVDFQLSGVA
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEE
VRSYRPPVRPSASGWPCVLYLHGGGWVVGGLDSHDFICCELAMALGAMVVVVDYRLAPEH
EECCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCHHHHHHHHHHHHEEEEEEEEECCCC
PFPAGFEDCLSVWRALRSGPFWFDPGRMLVAGDSAGGNLAAALCLALRDAGEPLPGAQVL
CCCCCHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEE
IYPGLGGDAQLPSRSECADAPLLASSDVDCYHALYLRGTAKPNAYAMPLLAGDFSGLPPA
EECCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHH
WIAVAQFDPLRDDGVCYAERLDAAGVDAALYYGEGLVHGCLRARHQVAEVDRLFENLLGF
HEEEEECCCCCCCCCHHHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MADKM
HHCCC
>Mature Secondary Structure 
GTYPLSPSMAAFVARTESFASDDSSLGGLRKSYDDMCRAFTPERPAGLEVVDFQLSGVA
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEE
VRSYRPPVRPSASGWPCVLYLHGGGWVVGGLDSHDFICCELAMALGAMVVVVDYRLAPEH
EECCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCHHHHHHHHHHHHEEEEEEEEECCCC
PFPAGFEDCLSVWRALRSGPFWFDPGRMLVAGDSAGGNLAAALCLALRDAGEPLPGAQVL
CCCCCHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEE
IYPGLGGDAQLPSRSECADAPLLASSDVDCYHALYLRGTAKPNAYAMPLLAGDFSGLPPA
EECCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHH
WIAVAQFDPLRDDGVCYAERLDAAGVDAALYYGEGLVHGCLRARHQVAEVDRLFENLLGF
HEEEEECCCCCCCCCHHHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MADKM
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]