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The map label for this gene is tnpB1 [H]

Identifier: 77454861

GI number: 77454861

Start: 265858

End: 267693

Strand: Direct

Name: tnpB1 [H]

Synonym: pREL1_0294

Alternate gene names: 77454861

Gene position: 265858-267693 (Clockwise)

Preceding gene: 77454860

Following gene: NA

Centisome position: 97.89

GC content: 63.34

Gene sequence:

>1836_bases
ATGAGCGTTCCGACAGACTCTTTGAGAAAACCTGAGTCAACCGCGGCCCGCACATACGTTCACGCGGTCACCAATGCACA
TGCCCACCGAACCCGACTCCGGGCTGTCCTGCGGTTCGAGGACAGGTTCGGTGATCTCGGCGCATGGAACGCGCTACCGG
TGTCGGCGCGCATGGCAGCTCCCGACATCGTGCGCGCGTTCGTTGCCTTCGCGGCCGTCTTCGAGTCGGTGGCCGTCGAT
GCGATCTATGTTGTGCAATCATCATCGAAATGGGGCGCCCATCTGGCCGAACGTGAACCCGTAGCTGCTTCCACATTCCG
CACGCAGGCAACAAGTTTGGGATTCTGCGAGCGCGAGGCAGGGAAGATGTGGTCCAAACTCGCGCAGATCTGTGTCATCG
CCGGCGCCACGACCGAGGCGCTCACCTCGGCGGAGTATGTCGCGGCCCGGGCCCAGTTTCATTCCGCCGTAGTCGCCAAG
GTCGGGCGTGCACCGAAATCGTTGAACGCGCCACTCTTTGGGCTCGATGCGCTCATGTTCCATCGCGGCCAAGGACCCCG
GCCACATCCGCGCAAACGCTGGGCAGCACGATCGGTGCCCGAGATCGGTTGGGACCGCGCCGAGGAGTGCGCGCCCGTCA
TGGTCGCTACGATGCGCCGTTACCTCGACCAGATCGGCATCAGCCTGCGGGTCTCGTCGGTGGACTGCATCGAGACCACA
CTGCGCCAGTTCGCCGGCCATCTGGCCACCACCAGCGACGTAGTCTGCGTCGCCGACATCGACCGCACCCACATCGAGGC
CTACAAGACCTGGTTGGCGGCACGGCCGGGGTATCGCAAGAACACGAACCTGTCCAAGACCACCATCGGCATGCGCATGG
GGCACCTGAGCGCGTTCTATCAACGCATCATCGAATGGGACTATCCTGATATCCCTGCCCGAGCGCCGGTCTACTCCTCG
GACCGGCCGATCAAGGACAAGCCGCTACCGCGATTCCTCGACGACGGCGCCGCCGCCAAGTTCTTGACCGCAGCCCGCAA
CCTGCCCGACGAGTTCGGTCGCCTCGCCATCGAAATGCTCTCACGTACCGGCATGCGCAAAGGCGAACTCCTCGACCTGA
CAATCGATTCGGTCGTCCAGATCGGTTCCGCCTACTGGCTCCGCATCCCCGTCGGCAAGCTCCACAACGACCGGTACGTC
CCCTTGCATCCCCAACTCAAAACAATGATCGACACCTGGCTCGAGCACCGACCGGACTGGCAGGACAGTCCGCTGCTCTT
CACCGACCGCGGTCGTCCGATCCCGGGCACCCGAATCGACAAGGCAGTCCAATCCGCTGCTGCCGCAGCGGGAATCGGCC
ACGTCCACCCTCATCAGCTGCGGCACACACTCGCGACGCAGGCCATCAACCGGGGTATGAGCCTCGAGGCGATCGCCGCA
TTGCTCGGGCACAAGACAATGGAGATGACGATGGTCTATGCCCGAATCGCGGACCGGACCGTTGCCGACCAATACTTCTC
CGTCACAGAGAAAGTCGAAGCCCTCTACAGTAAACACGAACCCGCCGTCCTGCCTGCCGAGGACGAGCCCGCCGCGATGC
GCAAACTCCGCGCCGAAGCCCACCGGCGGATGCTCGGTAACGGCTACTGCGCACGCCCAGTCGAACTCGACTGCCACTTC
GAGTCGATCTGCGAGTCATGCACGTTCTTCGTCACCACCATCGAGTTTCTCCCCACCCTCACCCGTCAGCGAGACGACGC
CGCAGACAAGGGCCAGATCGGGCGGCAGAAGGTCTTCGACGGACTTATCCAGCGACTCGACGACACGGGCACTTGA

Upstream 100 bases:

>100_bases
GCATCTATCTGCATCTGGCCAACGACTGGCTGATCGGCGAGTATTCCAAGGCGATGGACATTCTCGACGGCATCGCCGGT
GACATCCCTGGAGATGCGAA

Downstream 100 bases:

>100_bases
CATCGATCACCCACATAAGTTGTCGCAGCTTTGAGGATGGCGACGTCCTCACGCAGCTGCTTGTTCTCGGCCTTGAGCTT
TTTGATCTCCGCCGATTCCT

Product: putative transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MSVPTDSLRKPESTAARTYVHAVTNAHAHRTRLRAVLRFEDRFGDLGAWNALPVSARMAAPDIVRAFVAFAAVFESVAVD
AIYVVQSSSKWGAHLAEREPVAASTFRTQATSLGFCEREAGKMWSKLAQICVIAGATTEALTSAEYVAARAQFHSAVVAK
VGRAPKSLNAPLFGLDALMFHRGQGPRPHPRKRWAARSVPEIGWDRAEECAPVMVATMRRYLDQIGISLRVSSVDCIETT
LRQFAGHLATTSDVVCVADIDRTHIEAYKTWLAARPGYRKNTNLSKTTIGMRMGHLSAFYQRIIEWDYPDIPARAPVYSS
DRPIKDKPLPRFLDDGAAAKFLTAARNLPDEFGRLAIEMLSRTGMRKGELLDLTIDSVVQIGSAYWLRIPVGKLHNDRYV
PLHPQLKTMIDTWLEHRPDWQDSPLLFTDRGRPIPGTRIDKAVQSAAAAAGIGHVHPHQLRHTLATQAINRGMSLEAIAA
LLGHKTMEMTMVYARIADRTVADQYFSVTEKVEALYSKHEPAVLPAEDEPAAMRKLRAEAHRRMLGNGYCARPVELDCHF
ESICESCTFFVTTIEFLPTLTRQRDDAADKGQIGRQKVFDGLIQRLDDTGT

Sequences:

>Translated_611_residues
MSVPTDSLRKPESTAARTYVHAVTNAHAHRTRLRAVLRFEDRFGDLGAWNALPVSARMAAPDIVRAFVAFAAVFESVAVD
AIYVVQSSSKWGAHLAEREPVAASTFRTQATSLGFCEREAGKMWSKLAQICVIAGATTEALTSAEYVAARAQFHSAVVAK
VGRAPKSLNAPLFGLDALMFHRGQGPRPHPRKRWAARSVPEIGWDRAEECAPVMVATMRRYLDQIGISLRVSSVDCIETT
LRQFAGHLATTSDVVCVADIDRTHIEAYKTWLAARPGYRKNTNLSKTTIGMRMGHLSAFYQRIIEWDYPDIPARAPVYSS
DRPIKDKPLPRFLDDGAAAKFLTAARNLPDEFGRLAIEMLSRTGMRKGELLDLTIDSVVQIGSAYWLRIPVGKLHNDRYV
PLHPQLKTMIDTWLEHRPDWQDSPLLFTDRGRPIPGTRIDKAVQSAAAAAGIGHVHPHQLRHTLATQAINRGMSLEAIAA
LLGHKTMEMTMVYARIADRTVADQYFSVTEKVEALYSKHEPAVLPAEDEPAAMRKLRAEAHRRMLGNGYCARPVELDCHF
ESICESCTFFVTTIEFLPTLTRQRDDAADKGQIGRQKVFDGLIQRLDDTGT
>Mature_610_residues
SVPTDSLRKPESTAARTYVHAVTNAHAHRTRLRAVLRFEDRFGDLGAWNALPVSARMAAPDIVRAFVAFAAVFESVAVDA
IYVVQSSSKWGAHLAEREPVAASTFRTQATSLGFCEREAGKMWSKLAQICVIAGATTEALTSAEYVAARAQFHSAVVAKV
GRAPKSLNAPLFGLDALMFHRGQGPRPHPRKRWAARSVPEIGWDRAEECAPVMVATMRRYLDQIGISLRVSSVDCIETTL
RQFAGHLATTSDVVCVADIDRTHIEAYKTWLAARPGYRKNTNLSKTTIGMRMGHLSAFYQRIIEWDYPDIPARAPVYSSD
RPIKDKPLPRFLDDGAAAKFLTAARNLPDEFGRLAIEMLSRTGMRKGELLDLTIDSVVQIGSAYWLRIPVGKLHNDRYVP
LHPQLKTMIDTWLEHRPDWQDSPLLFTDRGRPIPGTRIDKAVQSAAAAAGIGHVHPHQLRHTLATQAINRGMSLEAIAAL
LGHKTMEMTMVYARIADRTVADQYFSVTEKVEALYSKHEPAVLPAEDEPAAMRKLRAEAHRRMLGNGYCARPVELDCHFE
SICESCTFFVTTIEFLPTLTRQRDDAADKGQIGRQKVFDGLIQRLDDTGT

Specific function: One of three proteins encoded by transposon Tn554 required for its transposition [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1790244, Length=226, Percent_Identity=24.3362831858407, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789261, Length=203, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109
- InterPro:   IPR004107 [H]

Pfam domain/function: PF02899 Phage_integr_N; PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 67904; Mature: 67772

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPTDSLRKPESTAARTYVHAVTNAHAHRTRLRAVLRFEDRFGDLGAWNALPVSARMAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCC
PDIVRAFVAFAAVFESVAVDAIYVVQSSSKWGAHLAEREPVAASTFRTQATSLGFCEREA
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHCCCCCHHHHHHHHHHHCCHHHHHH
GKMWSKLAQICVIAGATTEALTSAEYVAARAQFHSAVVAKVGRAPKSLNAPLFGLDALMF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
HRGQGPRPHPRKRWAARSVPEIGWDRAEECAPVMVATMRRYLDQIGISLRVSSVDCIETT
HCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHH
LRQFAGHLATTSDVVCVADIDRTHIEAYKTWLAARPGYRKNTNLSKTTIGMRMGHLSAFY
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
QRIIEWDYPDIPARAPVYSSDRPIKDKPLPRFLDDGAAAKFLTAARNLPDEFGRLAIEML
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCHHHHHHHHHHH
SRTGMRKGELLDLTIDSVVQIGSAYWLRIPVGKLHNDRYVPLHPQLKTMIDTWLEHRPDW
HHCCCCCCCEEEEEHHHHHHHCCEEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHCCCCC
QDSPLLFTDRGRPIPGTRIDKAVQSAAAAAGIGHVHPHQLRHTLATQAINRGMSLEAIAA
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
LLGHKTMEMTMVYARIADRTVADQYFSVTEKVEALYSKHEPAVLPAEDEPAAMRKLRAEA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
HRRMLGNGYCARPVELDCHFESICESCTFFVTTIEFLPTLTRQRDDAADKGQIGRQKVFD
HHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
GLIQRLDDTGT
HHHHHHCCCCC
>Mature Secondary Structure 
SVPTDSLRKPESTAARTYVHAVTNAHAHRTRLRAVLRFEDRFGDLGAWNALPVSARMAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCC
PDIVRAFVAFAAVFESVAVDAIYVVQSSSKWGAHLAEREPVAASTFRTQATSLGFCEREA
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHCCCCCHHHHHHHHHHHCCHHHHHH
GKMWSKLAQICVIAGATTEALTSAEYVAARAQFHSAVVAKVGRAPKSLNAPLFGLDALMF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
HRGQGPRPHPRKRWAARSVPEIGWDRAEECAPVMVATMRRYLDQIGISLRVSSVDCIETT
HCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHH
LRQFAGHLATTSDVVCVADIDRTHIEAYKTWLAARPGYRKNTNLSKTTIGMRMGHLSAFY
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
QRIIEWDYPDIPARAPVYSSDRPIKDKPLPRFLDDGAAAKFLTAARNLPDEFGRLAIEML
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCHHHHHHHHHHH
SRTGMRKGELLDLTIDSVVQIGSAYWLRIPVGKLHNDRYVPLHPQLKTMIDTWLEHRPDW
HHCCCCCCCEEEEEHHHHHHHCCEEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHCCCCC
QDSPLLFTDRGRPIPGTRIDKAVQSAAAAAGIGHVHPHQLRHTLATQAINRGMSLEAIAA
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
LLGHKTMEMTMVYARIADRTVADQYFSVTEKVEALYSKHEPAVLPAEDEPAAMRKLRAEA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
HRRMLGNGYCARPVELDCHFESICESCTFFVTTIEFLPTLTRQRDDAADKGQIGRQKVFD
HHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
GLIQRLDDTGT
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]