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The map label for this gene is insF1 [C]

Identifier: 77454836

GI number: 77454836

Start: 246283

End: 247110

Strand: Direct

Name: insF1 [C]

Synonym: pREL1_0269

Alternate gene names: 77454836

Gene position: 246283-247110 (Clockwise)

Preceding gene: 77454834

Following gene: 77454837

Centisome position: 90.69

GC content: 58.21

Gene sequence:

>828_bases
GTGCAGACGGCATGCCGGGTTCTGGAGGTGTCCGAGTCCGGTTTCTACGAGTGGCGAAACCGGCCACCCTCGGAACGCTC
ACTGAGACATGCCTGGCTGACACAGCTGATCACCGAGGTTCACACCGCCTCCCATGGCACCTACGGCGCCCGGCGTGTTC
ATGCCGAACTTACCCTTGGTCGCGGCGTTGCTGTTGGGCACGGCTGTGTCGAACTCTTGATGAGAACTGCTGGTGTGAAG
GGACTTCCGGGAAACAAACGTGCGCGTTCCAAGCATCAGACCCCGACCGCGAGCGATCTCGTGGACCGCAAATTCACCCG
CGACGAGCCGAACAAACTGTGGGTCACGGACATTACGGAACATCCCACGCGCGAAGGAAAAATCTACTGCGCTGTTGTCT
TGGACGCGTTTTCGAGGCGGGTAGTCGGTTGGTCGATTGACAGTTCTCAGACGGCGACGTTGGTGACGAACGCTCTGGGA
ATGGCTATTTCGAATCGGGGTCCGACGCCCGGTACGTTGATACATTCTGACCACGGAGTTCAGTTCATATCCTGGGCGTT
CACCCGCCGGGCGCACGACTCGGGCCTGGTCCCGTCGATGGGGTCGATCGGCGATTGTTATGACAATGCCGTCATCGAAT
CTTTCTGGGGCAGAATGCAAACCGAACTTCTGAACAGGAAACGATGGAAAACGAGGATCGAACTGGCAAACGCGATGTTC
GAGTACCTTGAGATCTTCCACAACCGGCAGCGTCGGCACAGCGCCCTCGGGATGCTGACCCCGATCGAGTTCGAGAACGT
ACACTTCACACGTCAACCTGTTGCCTGA

Upstream 100 bases:

>100_bases
ACCCTGGAGTCCGCCGAGCAGTGCACCCGCAGAAACACCAGACCAGCGTGAGCGTTGCCGCCCTTCGGTCCGTGCTTCTA
CACACCAATTAGGACTACCC

Downstream 100 bases:

>100_bases
AGTCAAATATCGCGACTACACGAAACTCGGGGCACATCACCACCGAACCCAGGGCGATTCACATGATGGAGGAATGAGGG
GATGCGCCCGAACAGGTACC

Product: putative transposase

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MQTACRVLEVSESGFYEWRNRPPSERSLRHAWLTQLITEVHTASHGTYGARRVHAELTLGRGVAVGHGCVELLMRTAGVK
GLPGNKRARSKHQTPTASDLVDRKFTRDEPNKLWVTDITEHPTREGKIYCAVVLDAFSRRVVGWSIDSSQTATLVTNALG
MAISNRGPTPGTLIHSDHGVQFISWAFTRRAHDSGLVPSMGSIGDCYDNAVIESFWGRMQTELLNRKRWKTRIELANAMF
EYLEIFHNRQRRHSALGMLTPIEFENVHFTRQPVA

Sequences:

>Translated_275_residues
MQTACRVLEVSESGFYEWRNRPPSERSLRHAWLTQLITEVHTASHGTYGARRVHAELTLGRGVAVGHGCVELLMRTAGVK
GLPGNKRARSKHQTPTASDLVDRKFTRDEPNKLWVTDITEHPTREGKIYCAVVLDAFSRRVVGWSIDSSQTATLVTNALG
MAISNRGPTPGTLIHSDHGVQFISWAFTRRAHDSGLVPSMGSIGDCYDNAVIESFWGRMQTELLNRKRWKTRIELANAMF
EYLEIFHNRQRRHSALGMLTPIEFENVHFTRQPVA
>Mature_275_residues
MQTACRVLEVSESGFYEWRNRPPSERSLRHAWLTQLITEVHTASHGTYGARRVHAELTLGRGVAVGHGCVELLMRTAGVK
GLPGNKRARSKHQTPTASDLVDRKFTRDEPNKLWVTDITEHPTREGKIYCAVVLDAFSRRVVGWSIDSSQTATLVTNALG
MAISNRGPTPGTLIHSDHGVQFISWAFTRRAHDSGLVPSMGSIGDCYDNAVIESFWGRMQTELLNRKRWKTRIELANAMF
EYLEIFHNRQRRHSALGMLTPIEFENVHFTRQPVA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=232, Percent_Identity=33.1896551724138, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI1788405, Length=272, Percent_Identity=33.4558823529412, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1787263, Length=272, Percent_Identity=33.4558823529412, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1786753, Length=272, Percent_Identity=33.4558823529412, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1786570, Length=272, Percent_Identity=33.4558823529412, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1786490, Length=272, Percent_Identity=33.4558823529412, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1789981, Length=272, Percent_Identity=31.6176470588235, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31047; Mature: 31047

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTACRVLEVSESGFYEWRNRPPSERSLRHAWLTQLITEVHTASHGTYGARRVHAELTLG
CCCHHHEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEEEEC
RGVAVGHGCVELLMRTAGVKGLPGNKRARSKHQTPTASDLVDRKFTRDEPNKLWVTDITE
CCEEHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEECCC
HPTREGKIYCAVVLDAFSRRVVGWSIDSSQTATLVTNALGMAISNRGPTPGTLIHSDHGV
CCCCCCCEEEEEEHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
QFISWAFTRRAHDSGLVPSMGSIGDCYDNAVIESFWGRMQTELLNRKRWKTRIELANAMF
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYLEIFHNRQRRHSALGMLTPIEFENVHFTRQPVA
HHHHHHHHHHHHHHHHCCCCCCEECCEEECCCCCC
>Mature Secondary Structure
MQTACRVLEVSESGFYEWRNRPPSERSLRHAWLTQLITEVHTASHGTYGARRVHAELTLG
CCCHHHEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEEEEC
RGVAVGHGCVELLMRTAGVKGLPGNKRARSKHQTPTASDLVDRKFTRDEPNKLWVTDITE
CCEEHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEECCC
HPTREGKIYCAVVLDAFSRRVVGWSIDSSQTATLVTNALGMAISNRGPTPGTLIHSDHGV
CCCCCCCEEEEEEHHHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
QFISWAFTRRAHDSGLVPSMGSIGDCYDNAVIESFWGRMQTELLNRKRWKTRIELANAMF
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYLEIFHNRQRRHSALGMLTPIEFENVHFTRQPVA
HHHHHHHHHHHHHHHHCCCCCCEECCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]