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The map label for this gene is pinE [H]

Identifier: 77454837

GI number: 77454837

Start: 247726

End: 248064

Strand: Direct

Name: pinE [H]

Synonym: pREL1_0270

Alternate gene names: 77454837

Gene position: 247726-248064 (Clockwise)

Preceding gene: 77454836

Following gene: 77454838

Centisome position: 91.22

GC content: 66.96

Gene sequence:

>339_bases
ATGGACGCCCTGCGCCCGGGAGACACGCTCGTCGTCTGGCGACTTGATCATCTCGGCCGCTCGCTGCCACACCTGATCGG
GACCGTCGCAGAGCTGGAGGCCCGCGGAGTGGCGTTCAAATCCCTGACCGAAGCGATCGACACGACCACGCCGGGCGGCA
AGCTGATCTTCCACATCTTCGGCGCCCTAGCGGAATTCGAGCGCAACCTCATCCGCGAACGCACCATCGCCGGGCTGTCG
GCGGCTCGTGATCGCGGCCGGGTCGGTGGCCGACCGTCTTCGCTGACGGCCGCGAAAAAGCGGCAGGCGAAAAAGATGCG
CGGCGAGGGCGTACCATGA

Upstream 100 bases:

>100_bases
GAATTACGGCTGGACGCACTCAATGCGGCCGGCTGTTTTCGCGTCTGGACAGACAAGGCATCAGGCGCGAAGACGGATCG
CCCTGAATTAGCGGCGTTGA

Downstream 100 bases:

>100_bases
CCGAAATCGCAGAAGTGTTGAACATTGCTCGCAGCACCCTGTACCGAAACCGGCCCTTCCGGATCCGGAGTGCGTAGCTG
GGGTTCGTCGGTGATGCGGT

Product: putative transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MDALRPGDTLVVWRLDHLGRSLPHLIGTVAELEARGVAFKSLTEAIDTTTPGGKLIFHIFGALAEFERNLIRERTIAGLS
AARDRGRVGGRPSSLTAAKKRQAKKMRGEGVP

Sequences:

>Translated_112_residues
MDALRPGDTLVVWRLDHLGRSLPHLIGTVAELEARGVAFKSLTEAIDTTTPGGKLIFHIFGALAEFERNLIRERTIAGLS
AARDRGRVGGRPSSLTAAKKRQAKKMRGEGVP
>Mature_112_residues
MDALRPGDTLVVWRLDHLGRSLPHLIGTVAELEARGVAFKSLTEAIDTTTPGGKLIFHIFGALAEFERNLIRERTIAGLS
AARDRGRVGGRPSSLTAAKKRQAKKMRGEGVP

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=109, Percent_Identity=55.9633027522936, Blast_Score=122, Evalue=7e-30,
Organism=Escherichia coli, GI1787827, Length=99, Percent_Identity=39.3939393939394, Blast_Score=69, Evalue=7e-14,
Organism=Escherichia coli, GI1787638, Length=99, Percent_Identity=39.3939393939394, Blast_Score=69, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 12128; Mature: 12128

Theoretical pI: Translated: 11.46; Mature: 11.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALRPGDTLVVWRLDHLGRSLPHLIGTVAELEARGVAFKSLTEAIDTTTPGGKLIFHIF
CCCCCCCCEEEEEEHHHHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHH
GALAEFERNLIRERTIAGLSAARDRGRVGGRPSSLTAAKKRQAKKMRGEGVP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDALRPGDTLVVWRLDHLGRSLPHLIGTVAELEARGVAFKSLTEAIDTTTPGGKLIFHIF
CCCCCCCCEEEEEEHHHHCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHH
GALAEFERNLIRERTIAGLSAARDRGRVGGRPSSLTAAKKRQAKKMRGEGVP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]