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The map label for this gene is istA [H]

Identifier: 77454775

GI number: 77454775

Start: 190964

End: 192505

Strand: Direct

Name: istA [H]

Synonym: pREL1_0208

Alternate gene names: 77454775

Gene position: 190964-192505 (Clockwise)

Preceding gene: 77454769

Following gene: 77454776

Centisome position: 70.32

GC content: 66.02

Gene sequence:

>1542_bases
ATGAAATCGAGCAGGGAGATCATGGAAATTTTGGAGGCGTACGACCTCACGGGTAGTTATCGGGCGGCGGCGGAGCTGGC
TGGGTGTGATCACCACACGGTGGCCCGGTATGTGCAGATGCGCGCTGCGGGGCAGCCGCCGGACCGCCGGCGGCATCGAG
CGCGGGCGATCGACGACTTTCTGCCGAAGATCGAGGAGTTGGTGGTCCGCTCGCAGGGCAAGGTCCGCGCGGATGTCATC
CACGAACGGATCGTCGCGCTCGGGTTCACCGGCGGTGAGCGGACCACGCGCCGGACGGTCGCGGAGGCGAAGGCCCAGTT
TCGGGCTGGTCGGCGGCGAGTGTATCGGCCGTGGGTAACCGAACCTGGGCTGTGGTTACAGTACGACTTCGGTGACGGGC
CAACGATTTCCGGCCGGAAGACGACGTTGTTCTGCGCATGGTTGGCGTGGTCGCGATTTCGGGTGGTGATCCCGATCTGG
GACAAGACATTGCCGACGGTGGCGGCGTGTTTGGATGCGACATTCCGCCGCTTGGGCGGGGTTCCGGTCTATGTCCTCAC
CGACAACGAGAAGACCGCGACGATCGATCACGTGGCTGGGATCGCGGTCCGCAATCCGGAGATCGTGGAGGTGGCACGGC
ATTACGGCACGACGCTGCGGACGTGTTTACCTGCTGATCCGGAGTCGAAGGGAGGAAGTGAGGCGACGGTGCGGATCGCC
AAAGCCGACCTGTTGCCGAAGGAGGTGAACCTGCGCGAGCAGTATCATTCTTTCGGTGAGCTCGAGTCGGCTTGCCGGCA
GTTCTGCACCGACGTCAACACCCGCGTCCACAGCTCTACTCGGCGGCGGCCGGTCGAGCGGCTGGCCGAAGAGTTGCAGC
GGCTGCATCCGCTGCCGAAATCTCCGTTCACCGCGGTGTTCGGCACCACCCGGCGGGTGAACTGGGAGTCGACGATCTCG
GTGGAAGGGGTGCGGTATTCGGTGCCGCACACGTTGATCGACACTCGGGTCTGGGCCCGCTTCCATGGTGAGGAGCTGAT
CGTGACCGCCGTCGACAATGATGGCGGTGCAGTCGAAGTCGCGCGGCACCGCCGCGGCCAACCCGGTTCACCGGTCCTCG
ACAACGACCACTACCCACCCCGCCCAGACCGCGACGCCGCCGACCGGGTGCCCAAGCCTCGTACCGCCGCCGAGCTCGCC
TTCCTACAGTTGGGCCAGGGTGCCAACAGCTGGCTGGTCGAGGCTGCCGCCGCGGGAGCGCGCCGCATCAGGGCGAAGAT
GGCCGAGGCCGTGGACCTTTCGAAGCTGCACTCCCCGGACGCAGTCGACAGAGCGTTGGGGACGGCGGCGATGACCGGCC
GGTTCGCCGATCGGGACCTGATCTCGATCCTCGACTACCAAGTCACCCACGAACACGCTGATCCGGTCCGTCGCAGCGAG
GACCACACTCTGCAACCGGGCACCGCCGCCTGGTCCACCTTCGGCCAGACACCAGCGCCGGCCAGCCCTGCCACTACTGA
CGATGAAAGCGATGACGCCTGA

Upstream 100 bases:

>100_bases
CCATTTATGGCGCATTTTGTGGCCATGGTTTCTCCCCGCGTACGGCCAGATAGTTCCCCACCTGCTGTGAGTCAGATTCG
GTCCCGGCAACGCTGGGTGG

Downstream 100 bases:

>100_bases
TGAGCACCCCTTTGCGCACTGTTACCAGCGCTAACGGCGACCCGTTGGCCGAGGCGATCGACCTGACTAAACGCCTGAAA
CTGCCCCATATCCGCAGGTC

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MKSSREIMEILEAYDLTGSYRAAAELAGCDHHTVARYVQMRAAGQPPDRRRHRARAIDDFLPKIEELVVRSQGKVRADVI
HERIVALGFTGGERTTRRTVAEAKAQFRAGRRRVYRPWVTEPGLWLQYDFGDGPTISGRKTTLFCAWLAWSRFRVVIPIW
DKTLPTVAACLDATFRRLGGVPVYVLTDNEKTATIDHVAGIAVRNPEIVEVARHYGTTLRTCLPADPESKGGSEATVRIA
KADLLPKEVNLREQYHSFGELESACRQFCTDVNTRVHSSTRRRPVERLAEELQRLHPLPKSPFTAVFGTTRRVNWESTIS
VEGVRYSVPHTLIDTRVWARFHGEELIVTAVDNDGGAVEVARHRRGQPGSPVLDNDHYPPRPDRDAADRVPKPRTAAELA
FLQLGQGANSWLVEAAAAGARRIRAKMAEAVDLSKLHSPDAVDRALGTAAMTGRFADRDLISILDYQVTHEHADPVRRSE
DHTLQPGTAAWSTFGQTPAPASPATTDDESDDA

Sequences:

>Translated_513_residues
MKSSREIMEILEAYDLTGSYRAAAELAGCDHHTVARYVQMRAAGQPPDRRRHRARAIDDFLPKIEELVVRSQGKVRADVI
HERIVALGFTGGERTTRRTVAEAKAQFRAGRRRVYRPWVTEPGLWLQYDFGDGPTISGRKTTLFCAWLAWSRFRVVIPIW
DKTLPTVAACLDATFRRLGGVPVYVLTDNEKTATIDHVAGIAVRNPEIVEVARHYGTTLRTCLPADPESKGGSEATVRIA
KADLLPKEVNLREQYHSFGELESACRQFCTDVNTRVHSSTRRRPVERLAEELQRLHPLPKSPFTAVFGTTRRVNWESTIS
VEGVRYSVPHTLIDTRVWARFHGEELIVTAVDNDGGAVEVARHRRGQPGSPVLDNDHYPPRPDRDAADRVPKPRTAAELA
FLQLGQGANSWLVEAAAAGARRIRAKMAEAVDLSKLHSPDAVDRALGTAAMTGRFADRDLISILDYQVTHEHADPVRRSE
DHTLQPGTAAWSTFGQTPAPASPATTDDESDDA
>Mature_513_residues
MKSSREIMEILEAYDLTGSYRAAAELAGCDHHTVARYVQMRAAGQPPDRRRHRARAIDDFLPKIEELVVRSQGKVRADVI
HERIVALGFTGGERTTRRTVAEAKAQFRAGRRRVYRPWVTEPGLWLQYDFGDGPTISGRKTTLFCAWLAWSRFRVVIPIW
DKTLPTVAACLDATFRRLGGVPVYVLTDNEKTATIDHVAGIAVRNPEIVEVARHYGTTLRTCLPADPESKGGSEATVRIA
KADLLPKEVNLREQYHSFGELESACRQFCTDVNTRVHSSTRRRPVERLAEELQRLHPLPKSPFTAVFGTTRRVNWESTIS
VEGVRYSVPHTLIDTRVWARFHGEELIVTAVDNDGGAVEVARHRRGQPGSPVLDNDHYPPRPDRDAADRVPKPRTAAELA
FLQLGQGANSWLVEAAAAGARRIRAKMAEAVDLSKLHSPDAVDRALGTAAMTGRFADRDLISILDYQVTHEHADPVRRSE
DHTLQPGTAAWSTFGQTPAPASPATTDDESDDA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017894
- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 57018; Mature: 57018

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSREIMEILEAYDLTGSYRAAAELAGCDHHTVARYVQMRAAGQPPDRRRHRARAIDDF
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LPKIEELVVRSQGKVRADVIHERIVALGFTGGERTTRRTVAEAKAQFRAGRRRVYRPWVT
HHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
EPGLWLQYDFGDGPTISGRKTTLFCAWLAWSRFRVVIPIWDKTLPTVAACLDATFRRLGG
CCCCEEEEECCCCCCCCCCCCEEEEEEEHHCCCEEEEEECCCHHHHHHHHHHHHHHHHCC
VPVYVLTDNEKTATIDHVAGIAVRNPEIVEVARHYGTTLRTCLPADPESKGGSEATVRIA
CEEEEEECCCCEEEHHHHHCEEECCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCEEEEEE
KADLLPKEVNLREQYHSFGELESACRQFCTDVNTRVHSSTRRRPVERLAEELQRLHPLPK
HHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
SPFTAVFGTTRRVNWESTISVEGVRYSVPHTLIDTRVWARFHGEELIVTAVDNDGGAVEV
CCCHHHHCCCEEECCCCEEEECCEEEECCHHHHHHHHHHEECCCEEEEEEECCCCCEEEE
ARHRRGQPGSPVLDNDHYPPRPDRDAADRVPKPRTAAELAFLQLGQGANSWLVEAAAAGA
HHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
RRIRAKMAEAVDLSKLHSPDAVDRALGTAAMTGRFADRDLISILDYQVTHEHADPVRRSE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
DHTLQPGTAAWSTFGQTPAPASPATTDDESDDA
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSSREIMEILEAYDLTGSYRAAAELAGCDHHTVARYVQMRAAGQPPDRRRHRARAIDDF
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LPKIEELVVRSQGKVRADVIHERIVALGFTGGERTTRRTVAEAKAQFRAGRRRVYRPWVT
HHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
EPGLWLQYDFGDGPTISGRKTTLFCAWLAWSRFRVVIPIWDKTLPTVAACLDATFRRLGG
CCCCEEEEECCCCCCCCCCCCEEEEEEEHHCCCEEEEEECCCHHHHHHHHHHHHHHHHCC
VPVYVLTDNEKTATIDHVAGIAVRNPEIVEVARHYGTTLRTCLPADPESKGGSEATVRIA
CEEEEEECCCCEEEHHHHHCEEECCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCEEEEEE
KADLLPKEVNLREQYHSFGELESACRQFCTDVNTRVHSSTRRRPVERLAEELQRLHPLPK
HHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
SPFTAVFGTTRRVNWESTISVEGVRYSVPHTLIDTRVWARFHGEELIVTAVDNDGGAVEV
CCCHHHHCCCEEECCCCEEEECCEEEECCHHHHHHHHHHEECCCEEEEEEECCCCCEEEE
ARHRRGQPGSPVLDNDHYPPRPDRDAADRVPKPRTAAELAFLQLGQGANSWLVEAAAAGA
HHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
RRIRAKMAEAVDLSKLHSPDAVDRALGTAAMTGRFADRDLISILDYQVTHEHADPVRRSE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
DHTLQPGTAAWSTFGQTPAPASPATTDDESDDA
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]