Click here to switch to the map view.

The map label for this gene is istB [H]

Identifier: 77454776

GI number: 77454776

Start: 192505

End: 193296

Strand: Direct

Name: istB [H]

Synonym: pREL1_0209

Alternate gene names: 77454776

Gene position: 192505-193296 (Clockwise)

Preceding gene: 77454775

Following gene: 77454783

Centisome position: 70.88

GC content: 64.77

Gene sequence:

>792_bases
ATGAGCACCCCTTTGCGCACTGTTACCAGCGCTAACGGCGACCCGTTGGCCGAGGCGATCGACCTGACTAAACGCCTGAA
ACTGCCCCATATCCGCAGGTCCTTGAGCGATATCATCCCGACCGCGAAAGCCCAACGCTGGGACCCCGCCGAAGTCGTGC
GGGTTCTCCTCGCTGAGGAAGCCGCCGGCCGGGACCGGGCGAACCTGCACACTCGCCGCAAACGCGCGGGTTTCCCTACC
GGCAAGACCTTCGGCGACTGGGACGAAACGAAATCGTCGATTCCCCGCGCCACCCAGGACGCGCTCCGCACCCTGGAGTG
GGTTGGGCGGCGTGAATGTTTCTGCGTCTGCGGGCCTTCGGGAACTGGCAAGTCTCACTTCACCGAGGCACTCGGGCAGG
CCGCAGTCGAAGCTGGGCTGGCGGTGTCTTGGTTCACCATCGAAGACCTCGGTGCCCTGGTCCGCCGGCACCGTGCCGAC
GACTCCATCGCCCGAGCCCTGACGAGGATCATCCGCTCCGACCTGATCATCGTCGACGACATCGGTCTGCTCCCGGTCTC
TGAAGACGCAGCCGAAGGACTCTATCGCCTCGTCGACGCTGCTTACGAACGCCGAGCGATTGCCGTCTCGAGCAACCTGC
ACCCCTCCGGCTTCGACGAGATCATGCCCAAAACATTGGCCACCGCCACCGTTGACCGGCTCCTGCACCATGCGCACGTC
GTCGTCACCGACGGCGACTCCTTCCGCCTCACCGAAGCCACCACCGGCAAGGGGGTGAAGCCCTTCAGCTAA

Upstream 100 bases:

>100_bases
ACCACACTCTGCAACCGGGCACCGCCGCCTGGTCCACCTTCGGCCAGACACCAGCGCCGGCCAGCCCTGCCACTACTGAC
GATGAAAGCGATGACGCCTG

Downstream 100 bases:

>100_bases
CCCAAATCAGAAAGCGGGTGGGGAAAACAACTGGCCGCCACCGGGGAGAACCCGTGGCCACCAGCGGGGAGAACTACTGG
CCGCCTACGGGGAGAACTTC

Product: transposition helper protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSTPLRTVTSANGDPLAEAIDLTKRLKLPHIRRSLSDIIPTAKAQRWDPAEVVRVLLAEEAAGRDRANLHTRRKRAGFPT
GKTFGDWDETKSSIPRATQDALRTLEWVGRRECFCVCGPSGTGKSHFTEALGQAAVEAGLAVSWFTIEDLGALVRRHRAD
DSIARALTRIIRSDLIIVDDIGLLPVSEDAAEGLYRLVDAAYERRAIAVSSNLHPSGFDEIMPKTLATATVDRLLHHAHV
VVTDGDSFRLTEATTGKGVKPFS

Sequences:

>Translated_263_residues
MSTPLRTVTSANGDPLAEAIDLTKRLKLPHIRRSLSDIIPTAKAQRWDPAEVVRVLLAEEAAGRDRANLHTRRKRAGFPT
GKTFGDWDETKSSIPRATQDALRTLEWVGRRECFCVCGPSGTGKSHFTEALGQAAVEAGLAVSWFTIEDLGALVRRHRAD
DSIARALTRIIRSDLIIVDDIGLLPVSEDAAEGLYRLVDAAYERRAIAVSSNLHPSGFDEIMPKTLATATVDRLLHHAHV
VVTDGDSFRLTEATTGKGVKPFS
>Mature_262_residues
STPLRTVTSANGDPLAEAIDLTKRLKLPHIRRSLSDIIPTAKAQRWDPAEVVRVLLAEEAAGRDRANLHTRRKRAGFPTG
KTFGDWDETKSSIPRATQDALRTLEWVGRRECFCVCGPSGTGKSHFTEALGQAAVEAGLAVSWFTIEDLGALVRRHRADD
SIARALTRIIRSDLIIVDDIGLLPVSEDAAEGLYRLVDAAYERRAIAVSSNLHPSGFDEIMPKTLATATVDRLLHHAHVV
VTDGDSFRLTEATTGKGVKPFS

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 28726; Mature: 28595

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPLRTVTSANGDPLAEAIDLTKRLKLPHIRRSLSDIIPTAKAQRWDPAEVVRVLLAEE
CCCCCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
AAGRDRANLHTRRKRAGFPTGKTFGDWDETKSSIPRATQDALRTLEWVGRRECFCVCGPS
HCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCC
GTGKSHFTEALGQAAVEAGLAVSWFTIEDLGALVRRHRADDSIARALTRIIRSDLIIVDD
CCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEC
IGLLPVSEDAAEGLYRLVDAAYERRAIAVSSNLHPSGFDEIMPKTLATATVDRLLHHAHV
CCCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCEE
VVTDGDSFRLTEATTGKGVKPFS
EEECCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
STPLRTVTSANGDPLAEAIDLTKRLKLPHIRRSLSDIIPTAKAQRWDPAEVVRVLLAEE
CCCCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
AAGRDRANLHTRRKRAGFPTGKTFGDWDETKSSIPRATQDALRTLEWVGRRECFCVCGPS
HCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCC
GTGKSHFTEALGQAAVEAGLAVSWFTIEDLGALVRRHRADDSIARALTRIIRSDLIIVDD
CCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEC
IGLLPVSEDAAEGLYRLVDAAYERRAIAVSSNLHPSGFDEIMPKTLATATVDRLLHHAHV
CCCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCEE
VVTDGDSFRLTEATTGKGVKPFS
EEECCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]