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The map label for this gene is nth [H]

Identifier: 75674404

GI number: 75674404

Start: 235058

End: 235816

Strand: Direct

Name: nth [H]

Synonym: Nwi_0205

Alternate gene names: 75674404

Gene position: 235058-235816 (Clockwise)

Preceding gene: 75674401

Following gene: 75674409

Centisome position: 6.91

GC content: 65.88

Gene sequence:

>759_bases
GTGAAGAAGAGAACCGGAAAGCCGCCGGCACGTCAGGCGGAGCCGGTCTCCGGGGTCAGGCGCGCCGTCAAGCCGGCGCG
AGGACCAGCGGAATTGCCGCCATCTCTCAGGCCATGGACGCCGGCTGAAGTCCACGAGGCCTTCACGCGCTTTCGCAGAG
CCAATCCCGAGCCGAAAGGCGAGCTCGAACACCTCAATCCCTTCACGTTGCTGGTCGCGGTGGTGCTGTCGGCCCAGGCG
ACCGACGCCGGCGTCAACAAGGCCACGCGCGCCTTGTTCGCTGTCGCCGACACGCCCGCCAGAATGCTGGCGCTCGGGGA
GGAGAAGGTCCGCGACTACATCAAGACCATCGGACTGTATCGCACCAAGGCGCGGAACATCATCGCGCTTTCGGAAAAAC
TGCTCGCGGAGTTTGACGGCGCGGTTCCACCCAGCAGGGCGGGGATCGAATCGCTGCCCGGCGCGGGACGCAAGACCGCC
AACGTCGTGCTCAACATGGCGTTCGGGGAGCGCACCATGGCGGTTGACACGCATGTGTTCCGGGTCGCCAACCGCACCGG
AATGGCGCCCGGCAAGACACCGCTCGAGGTCGAGCTCGGGCTTGAGCGCGTGATCCCCAACCAATTCATGCTGCATGCGC
ATCACTGGCTGATCCTGCACGGACGCTACACCTGCCTGGCGCGATCGCCGCGCTGCAAAGTGTGCCTGATCAACGACCTG
TGCCGCTGGCCCGAAAAGACCACAGAGGGCGCGGGGTGA

Upstream 100 bases:

>100_bases
GCCCGCCGCAGCCGGGACCGGCAAATCACTCGGGAAAGGCCCTGTAGCGGACCCTCGGCAGCAGGAACCCGCTGCGGCGA
CGGCAGGACACGCCAAGGGA

Downstream 100 bases:

>100_bases
TGATCGTCTCGTCCCGTTCGTACGCAGATACCTGCACCTGACCAGAACGGCGATCGCCGCGTTAAGTTCCGGCCGATTGA
ATCGCAAATCCTCGTTCGGG

Product: endonuclease III/Nth

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKKRTGKPPARQAEPVSGVRRAVKPARGPAELPPSLRPWTPAEVHEAFTRFRRANPEPKGELEHLNPFTLLVAVVLSAQA
TDAGVNKATRALFAVADTPARMLALGEEKVRDYIKTIGLYRTKARNIIALSEKLLAEFDGAVPPSRAGIESLPGAGRKTA
NVVLNMAFGERTMAVDTHVFRVANRTGMAPGKTPLEVELGLERVIPNQFMLHAHHWLILHGRYTCLARSPRCKVCLINDL
CRWPEKTTEGAG

Sequences:

>Translated_252_residues
MKKRTGKPPARQAEPVSGVRRAVKPARGPAELPPSLRPWTPAEVHEAFTRFRRANPEPKGELEHLNPFTLLVAVVLSAQA
TDAGVNKATRALFAVADTPARMLALGEEKVRDYIKTIGLYRTKARNIIALSEKLLAEFDGAVPPSRAGIESLPGAGRKTA
NVVLNMAFGERTMAVDTHVFRVANRTGMAPGKTPLEVELGLERVIPNQFMLHAHHWLILHGRYTCLARSPRCKVCLINDL
CRWPEKTTEGAG
>Mature_252_residues
MKKRTGKPPARQAEPVSGVRRAVKPARGPAELPPSLRPWTPAEVHEAFTRFRRANPEPKGELEHLNPFTLLVAVVLSAQA
TDAGVNKATRALFAVADTPARMLALGEEKVRDYIKTIGLYRTKARNIIALSEKLLAEFDGAVPPSRAGIESLPGAGRKTA
NVVLNMAFGERTMAVDTHVFRVANRTGMAPGKTPLEVELGLERVIPNQFMLHAHHWLILHGRYTCLARSPRCKVCLINDL
CRWPEKTTEGAG

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=220, Percent_Identity=28.1818181818182, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1787920, Length=201, Percent_Identity=60.6965174129353, Blast_Score=250, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=32, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=28.8770053475936, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=27.683615819209, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 27660; Mature: 27660

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRTGKPPARQAEPVSGVRRAVKPARGPAELPPSLRPWTPAEVHEAFTRFRRANPEPKG
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
ELEHLNPFTLLVAVVLSAQATDAGVNKATRALFAVADTPARMLALGEEKVRDYIKTIGLY
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHH
RTKARNIIALSEKLLAEFDGAVPPSRAGIESLPGAGRKTANVVLNMAFGERTMAVDTHVF
HHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCEEEEECCCCEEEHHHHHHH
RVANRTGMAPGKTPLEVELGLERVIPNQFMLHAHHWLILHGRYTCLARSPRCKVCLINDL
HHHHHCCCCCCCCCEEEECCHHHHCCHHHEEEECEEEEEECCEEEEECCCCCCEEEHHHH
CRWPEKTTEGAG
HCCCCCCCCCCC
>Mature Secondary Structure
MKKRTGKPPARQAEPVSGVRRAVKPARGPAELPPSLRPWTPAEVHEAFTRFRRANPEPKG
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
ELEHLNPFTLLVAVVLSAQATDAGVNKATRALFAVADTPARMLALGEEKVRDYIKTIGLY
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHH
RTKARNIIALSEKLLAEFDGAVPPSRAGIESLPGAGRKTANVVLNMAFGERTMAVDTHVF
HHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCEEEEECCCCEEEHHHHHHH
RVANRTGMAPGKTPLEVELGLERVIPNQFMLHAHHWLILHGRYTCLARSPRCKVCLINDL
HHHHHCCCCCCCCCEEEECCHHHHCCHHHEEEECEEEEEECCEEEEECCCCCCEEEHHHH
CRWPEKTTEGAG
HCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]